One of the key conditions for achieving superior regional growth is ensuring the harmonious development of both the layout and functions of territorial space. Territorial space, which includes production space, living space, and ecological space, serves as a critical system and venue for economic, cultural, and social activities in a region. The harmonized growth of production–living–ecological space (PLES) is essential for attaining sustainable development goals. Research on PLES offers a fresh perspective on promoting sustainable development of the spatial domain and the sustainable use of resources. However, studies on PLE functions in ecologically fragile areas are lacking. Therefore, in this study, which adopts a PLES perspective, land-use data are used to classify land according to the dominant functions of production, ecology, and living. Integration of point-of-interest (POI) data with socio-economic data was established to spatially describe indicators at the grid level and create a scoring system for PLES indicators in ecologically fragile areas. Finally, the entropy weight method, holistic assessment methods, coupling coordination degree model (CCDM), and geodetector were employed to explore the coupling coordination relationships and factors influencing PLESs in the Turpan–Hami Basin from 2010 to 2020. The results indicate that the Turpan–Hami Basin consists predominantly of potential ecological space, mainly in the central and northern regions, which are characterized by the Gobi Desert and bare rock landforms. Over the past decade, the PLES framework has seen a notable rise in the allocation of residential and ecological areas, whereas the portion dedicated to production spaces has diminished. The overall coupling coordination degree (CCD) of PLES in the Turpan–Hami Basin is at a coordinated level and gradually increasing. The most significant impact on the degree of PLES coupling coordination is exerted by population and natural factors. The research findings provide theoretical support for the sustainable utilization of resources in the Turpan–Hami Basin and other ecologically fragile areas while also offering scientific evidence to promote the coupling coordination of PLES, thereby contributing to high-quality regional development.