Hair Growth Length Research Articles

FORMULATION AND PHYSICAL QUALITY TESTS HAIR FERTILIZER PREPARATIONS FROM LEMONGRASS (Chrysopogon zizanioides L.) AND VCO (VIRGIN COCONUT OIL)

Hair loss is the shedding of hair from the skin. The factor that causes hair loss is a lack of nutrition for hair growth. Pure coconut oil (VCO) and citronella oil contain saturated fatty acids with short and medium chains of carbon atoms, especially octanoic acid and decanoic acid and essential oils which can be used to nourish hair. The aim of this research is to determine the effectiveness of the combination of citronella oil and VCO oil as a hair-fertilizing oil. This research was carried out using experimental methods including collecting and identifying samples, evaluating oil preparations, then testing hair-fertilizing oil preparations on 3 rabbits which were divided into 6 groups, namely positive control, negative control, formula 1, formula 2, formula 3 and formula 4. Data on the percentage of hair growth length were analyzed using the ANOVA (Analysis of Variance) method using the SPSS (Statistical Program for the Social Sciences) program. The results of the preparation evaluation showed that the oil preparation was stable during storage. The results showed that hair growth on day 28 with the positive control was 8.3 mm, negative control 4.17 mm, F1 4.83 mm, F2 5.17 mm, F3 6.33 mm and F4 7.83 mm. Duncan's test on day 28 showed that the positive control was not significantly different from formula 4, but was significantly different from formula 1, formula 2 and formula 3.

Estimation of the Frequency and Time of Human Exposure to Arsenic by Single Hair Analysis.

Arsenic (As) and its compounds are widely used in many applications. Long-term exposure to As can cause acute and chronic poisoning. In severe cases, it can lead to adverse effects, such as gene mutation, cell cancer and fetal malformation. The objective of this study was to accurately estimate As exposure frequency and time. Quantitative analysis of As in single hairs obtained from APL (acute promyelocytic leukemia) patients treated with As2O3 was performed by LA-ICP-MS. An informative As concentration distribution profile of single hair was applied to estimate the As exposure frequency and time. As exposure frequency was estimated according to the number of As concentration peaks. As exposure time was estimated according to the hair growth length in combination with the hair growth rate. The validation results demonstrate that this method was more efficient than the traditional method; compared with the traditional method, which provides estimates in months, our model shortened the As exposure time estimate to the range of a few days, which considerably improved the inference accuracy. Therefore, these results can be used for forensic toxicology studies, environmental exposure monitoring, etc.

Effect of Topical Grape Sap on Apoptosis in Hair Follicle Among Male Rats

Background: Hair loss is an emotional and stressful condition with an unpredictable profound impact on the social interactions of patients. Objectives: This study aimed to evaluate the effect of grape sap on apoptosis in hair follicles. Methods: This experimental study was performed on 126 male Wistar rats within a weight range of 30 ± 250 g. The rats were assigned into seven groups, namely bleomycin group, normal saline group, grape sap group (1 mg/kg), grape sap group (10 mg/kg), grape sap group (100 mg/kg), minoxidil group, and minoxidil plus grape sap group (100 mg/kg). The rats received bleomycin (1.7 mL/kg, four times with the interval of 5 days) and then were treated with grape sap for 21 days. The skin samples were taken from rats on days 7, 14, and 21 (i.e., the last day of the treatment). Results: The results showed a significant increase in the groups treated with grape sap, compared to the bleomycin-treated group in terms of the number of follicles, sebaceous glands, and blood vessels at the base of every follicle, hair growth length, total antioxidant capacity, and BCL2 gene expression. The use of grape sap showed beneficial effects on the reduction of hair fall. Conclusions: According to the results, it seems that grape sap can be employed as a non-chemical drug due to its rich compounds, especially antioxidants, and decreases apoptosis in hair follicle cells through increasing the expression ratio of BCL2/BAX, thereby stimulating hair growth.

Autologous activated platelet-rich plasma in hair growth: A pilot study in male androgenetic alopecia with in vitro bioactivity investigation.

Platelet-rich plasma (PRP) is effective in the treatment of androgenetic alopecia (AGA). The purpose of this study is to assess the effect of PRP on the proliferation of human follicle dermal papilla cells (HFDPCs), to observe the effect of PRP on the growth of hair follicles and hair shaft in vitro, to measure growth factors, and to evaluate the efficacy and safety of PRP injection. The effect of PRP on the proliferation of HFDPCs was observed. The length of hair follicle and hair shaft in vitro was measured. Then, the concentration of growth factors (EGF, FGF-2, FGF-7, IGF-1, HGF, PDGF-BB, and VEGF-A) was evaluated. Half-head injection of PRP was conducted to 10 males. Three treatments were conducted at 30-day intervals. Digital photographs were taken; hair diameter, hair density, unit density of hair follicles, and terminal hair/ vellus hair were analyzed. Platelet-rich plasma significantly promoted the proliferation of HFDPCs. Under the PRP culture, the hair follicle and hair shaft were grown, and the hair growth length on the 3rd and 6th days was greater than that of the control. PRP contained growth factors such as EGF, FGF-2, FGF-7, IGF-1, HGF, PDGF-B, and VEGF-A. Hair diameter, hair density, and unit hair follicle density on the PRP injection side peaked in the 6th month. The terminal hair/ vellus hair of the PRP injection side reached a peak in the 4th month. The average patient satisfaction during the entire treatment was 5.4 points (0-10 points). Platelet-rich plasma can promote hair growth. PRP injection is safe and effective for the treatment of AGA.

Molecular Pathways Involved in Promoting Activity of Timosaponin BII on Hair Growth in C57BL/6 Mice.

Hair loss is a common disease in dermatology, while the approved drugs may have unpredictable side effects. In this study, the effect of timosaponin BII extracted from Anemarrhena asphodeloides on hair growth of C57BL/6 mice was investigated by measuring the hair follicle morphology, hair growth length and area in C57BL/6 male mice, and the immunohistochemical analysis of β-catenin, Wnt3a, and Wnt10b in the dorsal skins of mice after topical application with minoxidil and timosaponin BII for 15 days. The decrease in skin brightness, the increase in the regrowing area of hair and hair follicles numbers, and the improvement of hair follicle morphology in the group applied with 0.5% timosaponin BII indicated an induction of the anagen phase in telogenic mice skin, which were comparative to the 2% minoxidil treatment. The immunohistochemical analysis detected an increase in the expression of β-catenin and Wnt10b, supporting the theory of the activation of the β-catenin/Wnt pathway was one of the pathways that are related to anagen phase induction. Anemarrhena asphodeloides is a herb commonly used for metabolic disorders in China. The present study is the first to show that the timosaponin BII, which is present at a high concentration in A. asphodeloides, promotes hair growth in C57BL/6 male mice. The results indicate that timosaponin BII may be a potential promoting agent for hair growth.

Mechanisms underlying the action of self-assembling short-peptide nano-fiber gel scaffold materials in the aesthetic repair of burn wounds

To study the mechanism of self-assembling short-peptide nano-fiber gel scaffolds on the aesthetic repair of burns. RADA16-1 assembled short-peptide nano-fiber gel scaffold was prepared using the ultrasonic method. Twenty female SD rats were randomly divided into two groups. Burn model was established using the electrical machinery method. The wound surface of the rats in the experimental group was coated with RADA16-1 and that of the rats in the control group was coated with NaCl. The degree of wound healing, hair follicle growth, hair growth length, and expression of bFGF and EGF were compared between the two groups. RADA16-1 fibers were broken into short fibers under the ultrasonic environment and then reorganized into gels. The degree of wound healing, hair follicle growth, hair growth length, and expression of bFGF and EGF in the experimental group were better than those in the control group. The assembly of short peptide nano-fiber gel scaffolds can effectively repair burn wounds.

Investigation of grape sap on wingless/integrated and β-catenin genes expression with histological factors on the hair follicle in rat.

Background and purpose:Natural products are used to improve the damage caused by harmful reagents in various pathological situations. This study investigated the effect of grape sap as a natural product with antioxidant properties on follicle cell proliferation in bleomycin (as a chemotherapy agent with toxic effects on hair growth) treated rats skin.Experimental approach:The bleomycin treated rats were administrated grape sap. Wingless/integrated (wnt) and β-catenin gene expression as follicle proliferative markers were evaluated using real-time polymerase chain reaction. Furthermore, histological factors and total antioxidant capacity were evaluated.Findings / Results:The data showed that, grape sap increased the number of anagenic hair follicle in grape sap (100 mg/kg) group (P < 0.001), sebaceous glands (P < 0.001), blood vessel density (P < 0.001), and hair growth length (P < 0.001). Also, wnt and β-catenin gene expression was elevated. The data showed that wnt and β-catenin gene expression were elevated in grape sap treated animals versus bleomycin group (P < 0.01 and 0.001, respectively).Conclusion and implications:Our finding showed that grape sap can be effective in increasing hair growth a gains bleomycin toxic effects on skin hair growth.

Accurate Estimation of Drug Intake Day by Microsegmental Analysis of a Strand of Hair by Use of Internal Temporal Markers.

Segmental hair analysis can be useful for estimating the time of drug intake. However, this estimation is currently only accurate to within several months. We previously conducted microsegmental analysis of a strand of hair to visualize drug distribution at a spatial resolution of 0.4 mm, which corresponds to daily hair-growth length. Herein, we describe a procedure for accurately estimating the day of drug intake by using internal temporal markers (ITMs) to mark a timescale in the analyzed strand of hair. Five drugs were administered in a single dose to the subjects, and then administration was stopped for several weeks. Two subsequent cycles of drug administration and similar withdrawal were performed. For analysis, a strand of hair was plucked from the subject's scalp. The first intake day was considered as the unknown and the drugs administered second and third were regarded as the ITMs. The first intake day was estimated based on the distance from hair root end to 3 drug peaks and 3 known days (hair sampling and 2 ITM cycles). The drug concentration-hair segment curve had 3 peaks, which reflected the 3 drug cycles. The use of ITMs reduced the error of the true intake day to within 2 days, because the growth rate of the analyzed strand of hair was accounted for by the 2 ITMs. The estimated accuracy showed little dependency on drug and individual variation. This procedure for estimating the time of drug intake down to a particular day can be used in drug-related crimes, drug abuse and compliance, and for medical diagnosis.

Microemulsion Formulation of Combination of Virgin Coconut Oil and Rice Bran Oil for Hair Growth

Research on micro emulsion formulation of combination of virgin coconut oil (VCO) and rice bran oil (RBO) as a hair growth promoter has been done. The purpose of this study was to formulate and produce the best formula that is physically stable and optimally promoting the hair growth by varying the content of VCO and RBO in the form of micro emulsions. These dosage formulations were prepared in three formulas using 45% tween 80 as surfactants and 20% glycerin as co surfactant i.e.: F,I containing a combination of VCO and RBO 1:1; FII (1:2) and FIII (2:1). Physical evaluation of the preparation was carried out for 8 weeks of storage which included organoleptic examination, pH, homogeneity, density, globule particle size, phase freeze thaw, and rabbit hair growth activity test for 18 days. Based on the results of the evaluation, it was found that the FI, FII and FIII micro emulsion formulations were physically stable for 8 weeks of storage with a pH ranging from 6.7 to 7.0, density of 1.085-1.088 g/mL, average globul particle size of FI (34.6 nm ), FII (54 nm) and FIII (68.8 nm). The best formulas is FIII for hair growth length of 1.26 cm for 18 days.

Effects of Sex Steroid Deprivation/Administration on Hair Growth and Skin Sebum Production in Transsexual Males and Females

To investigate androgen effects on the skin pilosebaceous unit, we studied 21 male-to-female transsexuals and 17 female-to-male transsexuals receiving cross-sex hormones. At baseline and after 4, 8, and 12 months, hair growth was evaluated by the Ferriman-Gallwey score; acne by the Leeds classification; hair growth rate, density, and shaft diameter by image analysis; and sebum production by Sebutape. In males, estrogens and antiandrogens reduced plasma testosterone to below 1.0 nmol/L. Though all parameters of hair growth and sebum production declined, facial hair growth continued. After 4 months, the decrease in shaft diameter had reached its maximum and seemed inversely associated with changes in hair growth length and density. In females, testosterone increased hair growth rate and sebum production. After 12 months, hairs on the cheek and abdomen had not yet reached diameters found in males. 5α-Androstane-3α,17β-diol glucuronide levels were only weakly associated with hair growth and sebum production. In conclusion, administration of estrogens and antiandrogens affects length and diameter of hairs at different rates. In the virtual absence of androgens, hair growth continues but at a slower rate. In women, after 12 months of androgen administration, hair diameters have not reached values of adult men.

Effects of sex steroid deprivation/administration on hair growth and skin sebum production in transsexual males and females.

To investigate androgen effects on the skin pilosebaceous unit, we studied 21 male-to-female transsexuals and 17 female-to-male transsexuals receiving cross-sex hormones. At baseline and after 4, 8, and 12 months, hair growth was evaluated by the Ferriman-Gallwey score; acne by the Leeds classification; hair growth rate, density, and shaft diameter by image analysis; and sebum production by Sebutape. In males, estrogens and antiandrogens reduced plasma testosterone to below 1.0 nmol/L. Though all parameters of hair growth and sebum production declined, facial hair growth continued. After 4 months, the decrease in shaft diameter had reached its maximum and seemed inversely associated with changes in hair growth length and density. In females, testosterone increased hair growth rate and sebum production. After 12 months, hairs on the cheek and abdomen had not yet reached diameters found in males. 5alpha-Androstane-3alpha,17beta-diol glucuronide levels were only weakly associated with hair growth and sebum production. In conclusion, administration of estrogens and antiandrogens affects length and diameter of hairs at different rates. In the virtual absence of androgens, hair growth continues but at a slower rate. In women, after 12 months of androgen administration, hair diameters have not reached values of adult men.