Hydroxyapatite Nanorods Research Articles

Hydroxyapatite Nanorods Function as Safe and Effective Growth Factors Regulating Neural Differentiation and Neuron Development.

Neural stem cell (NSC) transplantation is one of the most promising therapeutic strategies for neurodegenerative diseases. However, the slow spontaneous differentiation of NSCs often hampers their application in neural repair. Although some biological growth factors accelerate the differentiation of NSCs, their high cost, short half-life, and unpredictable behavior in vivo, as well as the complexity of the operation, hinder their clinical use. In this study, it is demonstrated that hydroxyapatite (HAp), the main component of bone, in the form of nanorods, can regulate the neural differentiation of NSCs and maturation of the newly differentiated cells. Culturing NSCs with HAp nanorods leads to the differentiation of NSCs into mature neurons that exhibit well-defined electrophysiological behavior within 5 days. The state of these neurons is much better than when culturing the cells without HAp nanorods, which undergo a 2-week differentiation process. Furthermore, RNA-sequencing data reveal that the neuroactive ligand-receptor interaction pathway is dominant in the enriched differentiated neuronal population. Hence, inorganic growth factors like HAp act as a feasible, effective, safe, and practical tool for regulating the differentiation of NSCs and can potentially be used in the treatment of neurodegenerative diseases.

Nano-rod hydroxyapatite for the uptake of nickel ions: Effect of sintering behaviour on adsorption parameters

Sorption studies in batch approach were carried out to investigate the elimination of Ni(II) ions from aqueous solution by fish scale derived nano-rod hydroxyapatite (FSDNHA) obtained via thermal decomposition at 800 and 900 oC respectively. The effects of Ni2+ concentration, contact time, pH, FSDNHA dose and temperature on elimination process were examined. Adsorption studies showed that maximum uptake amount of Ni2+ was attained at initial Ni2+concentration of 240 mg L-1, sorption time of 120 min, FSDNHA dose of 40 mg and solution pH of 6.0. The highest value of R2 obtained for Langmuir, Temkin, and Freundlich isotherms models are (0.996, 0.994, and 0.990) for FSDNHA-800 and (0.998, 0.986, and 0.992) for FSDNHA-900 respectively. The values of the maximum monolayer adsorption capacities obtained from Langmuir isotherm for FSDNHA-800 and FSDNHA-900 are 114.151 and 183.321 mg g-1 respectively. Kinetic modeling of the adsorption data clearly revealed that the sorption of Ni2+ onto the active sites of the FSDNHA surface was governed by Pseudo-first-order kinetic model. Thermodynamics parameters of Gibbs free energy (ΔG°), enthalpy (ΔH°) and entropy change (ΔS°) revealed that the sorption of Ni2+on FSDNHA was spontaneous, feasible and exothermic in nature. Well resolved peaks of a characteristic pure hydroxyapatite (HA) containing hydroxyl, phosphate and carbonate groups were obtained as the major functional groups responsible for the uptake of Ni2+, while the morphological analysis by FE-SEM and TEM revealed a nano-rod shape. This current study showed that Ni2+ removal by FSDNHA compared favorable with other adsorbents and is feasible.

De Novo Design of Functional Coassembling Organic-Inorganic Hydrogels for Hierarchical Mineralization and Neovascularization.

Synthetic nanostructured materials incorporating both organic and inorganic components offer a unique, powerful, and versatile class of materials for widespread applications due to the distinct, yet complementary, nature of the intrinsic properties of the different constituents. We report a supramolecular system based on synthetic nanoclay (Laponite, Lap) and peptide amphiphiles (PAs, PAH3) rationally designed to coassemble into nanostructured hydrogels with high structural integrity and a spectrum of bioactivities. Spectroscopic and scattering techniques and molecular dynamic simulation approaches were harnessed to confirm that PAH3 nanofibers electrostatically adsorbed and conformed to the surface of Lap nanodisks. Electron and atomic force microscopies also confirmed an increase in diameter and surface area of PAH3 nanofibers after coassembly with Lap. Dynamic oscillatory rheology revealed that the coassembled PAH3-Lap hydrogels displayed high stiffness and robust self-healing behavior while gas adsorption analysis confirmed a hierarchical and heterogeneous porosity. Furthermore, this distinctive structure within the three-dimensional (3D) matrix provided spatial confinement for the nucleation and hierarchical organization of high-aspect ratio hydroxyapatite nanorods into well-defined spherical clusters within the 3D matrix. Applicability of the organic–inorganic PAH3-Lap hydrogels was assessed in vitro using human bone marrow-derived stromal cells (hBMSCs) and ex vivo using a chick chorioallantoic membrane (CAM) assay. The results demonstrated that the organic–inorganic PAH3-Lap hydrogels promote human skeletal cell proliferation and, upon mineralization, integrate with the CAM, are infiltrated by blood vessels, stimulate extracellular matrix production, and facilitate extensive mineral deposition relative to the controls.

Improved Biocompatible, Flexible Mesh Composites for Implant Applications via Hydroxyapatite Coating with Potential for 3-Dimensional Extracellular Matrix Network and Bone Regeneration.

Hydroxyapatite (HA)-coated metals are biocompatible composites, which have potential for various applications for bone replacement and regeneration in the human body. In this study, we proposed the design of biocompatible, flexible composite implants by using a metal mesh as substrate and HA coating as bone regenerative stimulant derived from a simple sol–gel method. Experiments were performed to understand the effect of coating method (dip-coating and drop casting), substrate material (titanium and stainless steel) and substrate mesh characteristics (mesh size, weave pattern) on implant’s performance. HA-coated samples were characterized by X-ray diffractometer, transmission electron microscope, field-emission scanning electron microscope, nanoindenter, polarization and electrochemical impedance spectroscopy, and biocompatibility test. Pure or biphasic nanorod HA coating was obtained on mesh substrates with thicknesses varying from 4.0 to 7.9 μm. Different coating procedures and number of layers did not affect crystal structure, shape, or most intense plane reflections of the HA coating. Moduli of elasticity below 18.5 GPa were reported for HA-coated samples, falling within the range of natural skull bone. Coated samples led to at least 90% cell viability and up to 99.5% extracellular matrix coverage into a 3-dimensional network (16.4% to 76.5% higher than bare substrates). Fluorescent imaging showed no antagonistic effect of the coatings on osteogenic differentiation. Finer mesh size enhanced coating coverage and adhesion, but a low number of HA layers was preferable to maintain open mesh areas promoting extracellular matrix formation. Finally, electrochemical behavior studies revealed that, although corrosion protection for HA-coated samples was generally higher than bare samples, galvanic corrosion occurred on some samples. Overall, the results indicated that while HA-coated titanium grade 1 showed the best performance as a potential implant, HA-coated stainless steel 316 with the finest mesh size constitutes an adequate, lower cost alternative.

The structural and surface properties of Al-doped hydroxyapatite (Ca5(PO4)3OH) nanorods and their applications for pH-induced drug delivery

In this paper, we reported Al-doped hydroxyapatite (Al-HAp) nanorods with surface functionalization of hyaluronic acid (HA) for pH-responsive delivery of doxorubicin (DOX) for cancer treatment. The specific surface area and the crystal structure of both HAp and Al-HAp are studied. It is learned that the HAp and Al-HAp have similar crystal structures, but Al-HAp shows 10% larger specific surface area than HAp, which results in 30% higher DOX loading capacity than HAp nanorods, due to the increased surface area and enlarged pore size by Al doping. Moreover, the DOX loaded Al-HAp/HA was highly pH-responsive and exhibited outstanding biocompatibility and significantly enhanced anti-cancer killing effects.

Bioprocess-Inspired Room-Temperature Synthesis of Enamel-like Fluorapatite/Polymer Nanocomposites Controlled by Magnesium Ions.

Tooth enamel is composed of arrayed fluorapatite (FAP) or hydroxyapatite nanorods modified with Mg-rich amorphous layers. Although it is known that Mg2+ plays an important role in the formation of enamel, there is limited research on the regulatory role of Mg2+ in the synthesis of enamel-like materials. Therefore, we focus on the regulatory behavior of Mg2+ in the fabrication of biomimetic mineralized enamel-like structural materials. In the present study, we adopt a bioprocess-inspired room-temperature mineralization technique to synthesize a multilayered array of enamel-like columnar FAP/polymer nanocomposites controlled by Mg2+ (FPN-M). The results reveal that the presence of Mg2+ induced the compaction of the array and the formation of a unique Mg-rich amorphous-reinforced architecture. Therefore, the FPN-M array exhibits excellent mechanical properties. The hardness (2.42 ± 0.01 GPa) and Young's modulus (81.5 ± 0.6 GPa) of the as-prepared FPN-M array are comparable to those of its biological counterparts; furthermore, the enamel-like FPN-M array is translucent. The hardness and Young's modulus of the synthetic array of FAP/polymer nanocomposites without Mg2+ control (FPN) are 0.51 ± 0.04 and 43.5 ± 1.6 GPa, respectively. The present study demonstrates a reliable bioprocess-inspired room-temperature fabrication technique for the development of advanced high-performance composite materials.

Mesoporous Mn-doped hydroxyapatite nanorods obtained via pyridinium chloride enabled microwave-assisted synthesis by utilizing Donax variabilis seashells for implant applications

Manganese-doped mesoporous hydroxyapatite (MnHAp) nanorods, a bio-apatite were synthesized via pyridinium chloride mediated microwave approach using bio-waste Donax variabilis seashells to treat orthopedic infections. This is the first report on using pyridinium chloride mediated mesoporous MnHAp nanorods synthesis. Pure and Mn doped HAp samples were examined using Raman spectroscopy, X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) studies to confirm the prepared HAp nanorods. Furthermore, the fabrication of manganese-doped HAp was successful with the formation of a hexagonal crystal lattice without disturbing the HAp phase. It is because, at the time of synthesis, PO43− ions form an electrostatic interaction with the Mn ions. Furthermore, Mn-doped HAp samples showed a reduction in their sizes of 15, 10–15, 5–10 nm width, and 80–100, 10–15, 20–30 nm length with varied pore diameters and surface area. The pure HAp, MnHAp-1, MnHAp-2, and MnHAp-3 nanorods disclose the surface area of 39.4, 18.0, 49.2, and 80.4 m2 g−1, with a pore volume of 0.0102, 0.0047, 0.0143, and 0.0447 cm3 g−1, the corresponding pore diameter was estimated to be 6, 7, 6, and 4 nm, respectively. Moreover, antibacterial activity reveals effective bactericidal action against infections causing pathogens whereas cytotoxicity examination (MTT assay), and zebrafish results reveal their non-toxic behavior. Therefore, it is evident from the study, that rapid fabrication of mesoporous and diverse structured MnHAp nanorods could be convenient with pyridinium chloride enabled microwave-assisted method as a bactericidal biomaterial for implant applications.

Polymer brush grafted antimicrobial peptide on hydroxyapatite nanorods for highly effective antibacterial performance

Serious bacterial infection induces the failure of artificial implants. Covalent bonding of antimicrobial peptides (AMPs) on implant surfaces can avoid the main shortcoming (blast releasing) by physisorption, but the low grafting yield, mobility, and stability of bonded AMPs limit their antibacterial efficiency. In this study, hydroxyapatite (HA) nanorods co-doped with Fe and Si were fabricated on Ti based on previous work, and the antimicrobial peptide HHC-36 was chemically bonded on the nanorods with and without polymer brushes as a spacer. The microstructures and physicochemical properties of coating surfaces were observed; Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and hFOB1.19 behaviours in the presence of in vitro antibacterial activity, and the immunization response in vivo on various surfaces were evaluated. The results show that the grafting of polymer brushes and HHC-36 did not substantially change the microstructure of the nanorods, but the brushes efficiently increased the load and stability of the HHC-36. The HA nanorods did not kill bacteria, but with the assistance of HHC-36, physical puncturing by nanorods worked efficiently in killing S. aureus. In contrast with Ti, in phosphate-buffered saline and suppressed biofilm formation in a nutrient-rich medium, HA nanorods with polymer-brush-grafted HHC-36 killed 99.5% of S. aureus and 99.9% of E. coli. This outstanding antibacterial activity was attributed to the synergistic effect of AMP-derived destruction and the physical puncturing by HA nanorods. Also, HA nanorods with polymer-brush-grafted HHC-36 showed cytocompatibility in vitro, and in vivo they inhibited bacterial infection and reduced the inflammatory response, indicating that polymer-brush-grafted HHC-36 on HA nanorods has great potential application in surface modification of Ti implants, especially in infected cases.

Size Analysis Based on Sorption Study Data for Hydroxyapatite Nanoparticles

This work is devoted to the study on the possibility of applying the method of analyzing the particle size of hydroxyapatite at various stages of synthesis. A series of hydroxyapatite powders obtained by chemical deposition using microwave radiation is considered. Determination of the size of hydroxyapatite nanorods is carried out on the basis of data on the density and specific surface area. The specific surface area is analyzed by the sorption method. Determination of the ratio "length / diameter" of nanorods is based on the analysis of images obtained by scanning electron microscopy.

Repair calvarial defect of osteoporotic rats by berberine functionalized porous calcium phosphate scaffold.

In this article, we propose a simple scheme of using berberine (BBR) to modify porous calcium phosphate ceramics (named PCPC). These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC. We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images. The microenvironment of PCPC surface is essential for promoting BMSCs’ proliferation and differentiation. These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats. Moreover, PCPC/BBR had significantly increased the expression levels of ALP, osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats.

Preparation of ROS active and photothermal responsive hydroxyapatite nanoplatforms for anticancer therapy

Photothermal responsive nanoplatforms are attracting for photothermal therapy (PTT) of cancer. Herein, we propose a strategy to prepare IR-780 modified hydroxyapatite (HAP) nanorods as photothermic agents (HAP@IR-780). The results demonstrated that the obtained HAP@IR-780 was photothermal responsive under near-infrared laser irradiation the photothermal conversion efficiency was 69.3%, and it remained photostability after 4 cycles of irradiation. This advantage overcomes the optical instability of IR780. MTT and cellular uptake research proved that HAP@IR-780 was biocompatible in appropriate concentration range (0–20 μg/mL) without laser irradiation. Concentration-dependent internalization and reactive oxygen species (ROS) related apoptosis of HAP@IR-780 for MCF-7 cells were observed. Animal experiments showed that the gathered HAP@IR-780 at the tumor site reached a photothermal responsive temperature up to 57.9 °C, which could almost ablate the tumor with volumes as large as 1500 mm3. In general, our photothermal material has good photothermal conversion characteristics, and may have the least safety problems while showing excellent therapeutic effects. Therefore, HAP@IR-780 has a brilliant prospect in the field of tumor photothermal therapy.

In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

IntroductionIt is urgently needed to develop composite bone scaffold with excellent mechanical properties and bioactivity in bone tissue engineering. Combining graphene oxide (GO) and hydroxyapatite (HAP) for the reinforcement of biopolymer bone scaffold has emerged as a promising strategy. However, the dispersion of GO and HAP remains to be a big challenge. ObjectivesIn this present work, the mechanical properties of GO and the bioactivity of and HAP were combined respectively via in situ synthesis for reinforcing biopolymer bone scaffold. MethodsGO nanosheets were employed to in situ synthesize GO-HAP nanocomposite via hydrothermal reaction, in which their abundant oxygen-containing groups served as anchor sites for the chelation of Ca2+ and then Ca2+ absorbed HPO42- via electrovalent bonding to form homogeneously dispersed HAP nanorods. Thereby, the GO-HAP nanocomposite was blended with biopolymer poly-L-lactic acid (PLLA) for fabricating biopolymer scaffold by selective laser sintering (SLS). ResultsGO nanosheets were uniformly decorated with HAP nanorods, which were about 60 nm in length and 5 nm in diameter. The compressive strength and modulus of PLLA/12%GO-HAP were significantly increased by 53.71% and 98.80% compared to the pure PLLA scaffold, respectively, explained on the base of pull out, crack bridging, deflection and pinning mechanisms. Meanwhile, the mineralization experiments indicated the PLLA/GO-HAP scaffold displayed good bioactivity by inducing the formation of apatite layer. Besides, cell culturing experiments demonstrated the favorable cytocompatibility of scaffold by promoting cell adhesion and proliferation. ConclusionsThe present findings show the potential of PLLA/GO-HAP composite scaffold via in situ synthesis in bone tissue engineering.

Microwave-assisted synthesis of nanorod hydroxyapatite from eggshells

Microwave-assisted synthesis of nanorod hydroxyapatite from eggshells

Investigation on the Effect of Hydroxyapatite Nanorod on Cement Hydration and Strength Development.

This work investigated the effect of hydroxyapatite (HA) nanorods on the strength development and hydration of cement. Undispersed HA nanorods (HA-UD) and dispersed HA nanorods (HA-DN) were prepared by atom-efficient neutralization. The strength of mortars modified by HA nanorods was tested, as well as their compatibility with supplementary cementitious material. The hydration of HA-modified cement pastes was characterized via in situ X-ray diffraction, isothermal calorimetry and scanning electron microscopy. As the results suggest, the undispersed HA-DN caused a considerable increase in superplasticizer demand to achieve the same level of flow. Both HA nanorods showed a significant accelerating effect on early hydration, with approximately 100% strength enhancement at 12 h at 2.0% dosage. The effect on early strength of the nanorods is retained in systems with up to 30% fly ash in the binder mass. According to the characterizations, the rate of the hydration reaction in the acceleration period was enhanced by HA nanorods, and C3S consumption was also increased. In all of the testing situations, HA-DN showed superior performance, likely due to improved spatial distribution of the hydroxyapatites. The results suggest that proper dispersion of the nanorods is necessary to optimize its performance.

RETRACTED: Synthesis of strontium (Sr) doped hydroxyapatite (HAp) nanorods for enhanced adsorption of Cr (VI) ions from wastewater

The development of high-efficiency adsorbents for heavy metal ion removal from wastewater is highly desirable and challenging due to their synthesis complexity and low adsorption capacities. Herein, we reported the synthesis of strontium (Sr) doped hydroxyapatite (HAp) for the increased Cr (VI) adsorption. The effects of pH, temperature, and time on adsorption performances were studied. As a result, the Sr-HAp nanorods can achieve a Cr (VI) adsorption capacity of 443 mg/g, which is significantly higher than that of HAp nanorods (318 mg/g). To better understand the adsorption mechanism, the Langmuir isotherm model was established. The modeling results indicated that Langmuir monolayer chemical adsorption contributed to the efficient Cr (VI) ion removal for Sr-HAp nanorods adsorbents. The surface area and surface functional groups (O–H) contributed to the different Cr (VI) adsorption capacities between HAp and Sr-HAp.

Incorporation of 5-Nitroisatin for Tailored Hydroxyapatite Nanorods and its Effect on Cervical Cancer Cells: A Nanoarchitectonics Approach

Hydroxyapatite (HAP) have been widely used as bone implants for its biocompatibility, and bioactivity. Other approaches like scaffold development, drug delivery and nanomaterials preparation are explored recently. Herein, hydroxyapatite incorporated with 5-nitroisatin (HAP-5 N) were prepared and tested for its anticancer potential. The prepared HAP-5 N were found to have rod like structures as seen using scanning electron microscopic analysis. The structural elucidation of 5-nitroisatin were confirmed by performing 2D heteronuclear single quantum correlation experiment. The synthesized HAP-5 N were assessed for its possible role as an anticancer agent against cervical cancer (SiHa) cells. From the study, it was found that HAP-5 N is efficient in inhibiting the growth of cervical cancer cell lines.

The effect of chemical structure of carboxylate molecules on hydroxyapatite nanoparticles. A structural and morphological study

Being the most abundant non-macromolecular organic component of bone, the role of citrate (Cit) in hydroxyapatite (HA) crystallization is of high relevance. In this work we have investigated the influence of hydroxycitrate (CitOH) and glutarate (Glr) on HA crystallization in terms of particle growth, composition, and morphology in comparison to Cit. CitOH and Glr have been selected for this work because they share the same backbone structure of Cit but bear different functional groups in the central region. Our data has revealed that CitOH strongly inhibits HA crystallization more efficiently than Cit. CitOH-HA nanoparticles are composed of platy, elongated particles similar to those of Cit-HA but they are ca. twice smaller and have a lower crystal order. On the other hand, Glr does not inhibit HA crystallization as Cit, but leads to the formation of OCP platelets that convert with maturation time to HA nanorods with larger aspect ratio than Cit-HA. In comparison to Cit-HA samples, Glr-HA nanoparticles have bigger dimensions, and higher structural order. Overall, our data reveal that the central carboxyl group of Cit is involved in the selective binding with HA crystal surface and in regulating HA crystal growth. The results of this work highlight new possibilities to control the formation of HA for designing advanced bioactive materials and give new insights on the role of the structure of Cit in regulating the HA morphology.

Functionalized multi-walled carbon nanotubes and hydroxyapatite nanorods reinforced with polypropylene for biomedical application

Modified multi-walled carbon nanotubes (f-MWCNTs) and hydroxyapatite nanorods (n-HA) were reinforced into polypropylene (PP) with the support of a melt compounding approach. Varying composition of f-MWCNTs (0.1–0.3 wt.%) and nHA (15–20 wt.%) were reinforced into PP, to obtain biocomposites of different compositions. The morphology, thermal and mechanical characteristics of PP/n-HA/f-MWCNTs were observed. Tensile studies reflected that the addition of f-MWCNTs is advantageous in improving the tensile strength of PP/n-HA nanocomposites but decreases its Young’s modulus significantly. Based on the thermal study, the f-MWCNTs and n-HA were known to be adequate to enhance PP’s thermal and dimensional stability. Furthermore, MTT studies proved that PP/n-HA/f-MWCNTs are biocompatible. Consequently, f-MWCNTs and n-HA reinforced into PP may be a promising nanocomposite in orthopedics industry applications such as the human subchondral bone i.e. patella and cartilage and fabricating certain light-loaded implants.

Luminescent net-like inorganic scaffolds with europium-doped hydroxyapatite for enhanced bone reconstruction.

Bone reconstruction is an urgent problem during clinical treatment. In the past few decades, the construction of composite scaffolds has been a hot spot in the research field of bone tissue engineering (BTE). However, the disadvantages of composite materials raise our awareness to explore the potential application of hydroxyapatite (HAp) in bone substitutes due to the closest properties of HAp to natural bone tissue. In our study, we synthesized Eu3+-doped HAp (HAp:Eu3+) ultralong nanowires, which can be transformed to hydrophilic net-like scaffolds via a thiol-ene click reaction. The property of luminescence of HAp from Eu3+ is beneficial for identifying the relative position of materials and bone marrow mesenchymal stem cells (BMSCs). HAp:Eu3+ scaffolds with excellent cell biocompatibility could promote the expression of early bone formation markers (ALP and ARS) and enhance the expression of genes and proteins associated with osteogenesis (Runx 2, OCN, and OPN). In the end, the results of the in vivo osteogenesis experiment showed that pure HAp scaffolds presented different effects of bone tissue reconstruction compared with the composite scaffolds with HAp nanorods and polymer materials. The superior osteogenic effect could be observed in net-like pure HAp scaffold groups. Furthermore, the absorption of HAp:Eu3+ scaffolds could be monitored due to the luminescence property of Eu3+. This strategy based on ultralong HAp nanowires proved to be a new method for the construction of simple reticular scaffolds for potential osteogenic applications.

Rapid structural regulation, apatite-inducing mechanism and in vivo investigation of microwave-assisted hydrothermally treated titania coating.

Owing to the poor bioactivity of microarc oxidation (MAO) coating and the rapid activation ability of the microwave hydrothermal (MH) technique, MH treatment was applied to optimize the in vivo interface status between MAO-treated titanium and bone. In this study, consequently, new outermost layers were prepared using hydroxyapatite (HA) nanorods, HA submicron pillars or sodium titanate nanosheets. The results revealed that the NaOH concentration significantly influenced the surface structure and phase constitution of the MAO samples. Moreover, on enhancing the NaOH concentration, the number of HA phases was decreased. Further, the influence of the NaOH concentration on the interfacial bonding strength was insignificant for concentrations ≤0.5 mol L−1. Transmission electron microscopy (TEM) analysis showed that the induction of apatite was accompanied by the dissolution of the HA crystals and there was excellent crystallographic matching with the HA crystals. The in vitro and in vivo analyses revealed that the MH-treated MAO sample with the HA nanorods possessed superior apatite-formation ability and osseointegration, including a small amount of soft tissue and optimal bone–implant interfacial bonding force, thus signifying strong potential for the optimization of the bone–implant interfacial status.