Gymnema Sylvestre Research Articles

Genetic variability and heritability of growth, yield and quality traits in Gymnema sylvestre: an anti-diabetic medicinal herb

Genetic variability and heritability of growth, yield and quality traits in <i>Gymnema sylvestre</i>: an anti-diabetic medicinal herb

A Comprehensive Review on the Role of &lt;i&gt;Gymenma sylvestre&lt;/i&gt; in Combating Diabetes-2

The current review focuses on a detailed comprehensive role of Gymnema sylvestre (GS) and other herbs for their Ayurvedic impact in controlling blood sugar by releasing insulin from the pancreas in diabetic Type 2. G. sylvestre (GS) supplementation has also been seen to leave an impact on various cardiovascular risk factors and improves the lipid profile, blood pressure, anthropometric indices, and control of glycemia. Additionally, extracts of Gymnema have been shown to have an inhibitory impact on triglyceride accumulation in muscles and the liver, while reducing the buildup of fatty acids in the bloodstream, thus also reducing HB1C parameters. In the current review, we have also discussed information about Gymnemic acid structure isolation their sources, their clinical effects in diabetic control in Type 1 and Type 2, and clinical effects over the humans and mice signalling as immunomodulators.

Ethnopharmacological Importance of Gymnema sylvestre

Gymnema sylvestre is one of the valuable medicinally important herbs that belong to family Apocynaceae. G. Sylvester is a woody climber mostly found in India, Srilanka, China, Indonesia, Malaysia and Tropical Africa. Traditionally the leaf of the herb is used as antidiabetic, antihelmentic, antiobesity, anti-inflammatory, hypolipidaemic, antivenom and antimicrobial. The herb is used by some ethnic community in constipation, haemorrhoids, jaundice, leucoderma, asthma, bronchitis, cardiac problems and dyspepsia. Most important phytochemicals of the plant are gymnemic acid, deacyl gymnemic acid and gymnemagenol which help in the controlling of blood cholesterol, triglyceride levels, inflammation and body weight. The present review aims to document the traditional and modern pharmacological uses of the plant to provide better scope for further experiments and future application. The botanical description, cultivation practices, phytochemical constituents and safety issues of the plant has also been described in the chapter.

An ethnobotanical survey of medicinal plants used by diabetes patients attending the Teaching Hospital, Jaffna, Sri Lanka

The scope of this study is to describe the ethnobotany of medicinal plants used in the management of diabetes mellitus (DM) among diabetic patients attending the Teaching Hospital, Jaffna. A cross-sectional descriptive study was conducted among 388 patients attending the medical clinic of Teaching Hospital, Jaffna, using systematic sampling technique. Data were collected using a validated structured interviewer-administered questionnaire and were analysed to generate the summary of the percentages using descriptive statistics. Family Important Value (FIV) and Related Frequency Citation (RFC) were calculated to quantitatively determine the common consent between the informants on the use of endemic medicinal plants in the division. A total of 59 species belonging to 33 families were reported by 329 diabetic patients with the response rate of 100%. Among them Moringa oleifera Lamk. (142) was stated as the species with the highest RFC value, followed by Gymnema sylvestre (105), Momordica charantia Linn. (104). Moringaceae was the most dominant family with FIV 43.16. The value of RFC of the plants mentioned in this survey varied from 0.003 to 0.432. Moringa oleifera Lamk. showed the highest RFC value (0.432). In this study, 29 plant species used for DM management were reported for the first time in Sri Lanka. Further, antidiabetic property of Achyranthus polygonoides, Argyreia pomacea Wall. ex Choisy and Cassia tomentosa are yet to be investigated. These findings facilitate the documentation and the conservation of medicinal plants used for DM by the patients in Teaching Hospital, Jaffna, Sri Lanka. Moreover this study open doors for phytochemical screening and identification of new antidiabetic active compounds.

A Review on Endangered Medicinal Plants of Melghat Forest in Amravati District

The Melghat Forest, located in the Western Ghats region of Maharashtra, is known for its rich biodiversity including a variety of plant and animal species. However, like many other forested area, the Melghat Forest is facing threats from various anthropogenic activities, such as deforestation, habitat destruction, and the introduction of exotic species. (Sharma, 2015) To address these challenges, the state government has implemented various conservation measures, including the establishment of a threatened fauna management framework. Some of these endangered medicinal plants are Saussaureea lappa, Picorrhiza kurroa, Ginkgo Biloba, Swertia chirata,Gymnema sylvestre,Tinospora cordifolia,Salaca oblonga,Holostemma,Celastrus paniculata,Oroxylum indicum, Glycyrrhiza glabra,Typophora indica,Bacopa monnieri,Rauwolfia serpentine. The most important species in these forests is Teak(Tectona Grandis).Teak forests occupy over 11500 sq.km.area which is about 1/5 of the area under forest department.The present investigation of some of the plants which are more important to human healthcare and may be extinct in future.

Enhanced Antidiabetic Effects of Gymnema sylvestre and Probiotics in Alloxan-Induced Diabetic Rats

Abstract Introduction/Objective Introduction: Gymnema sylvestre is a well-known herbal remedy for diabetes, with its anti-diabetic properties attributed to gymnemic acids isolated from its leaves. Probiotics, such as Lactobacillus acidophilus and Lactobacillus casei, have also shown potential in managing diabetes. This study aims to evaluate the combined antidiabetic effects of G. sylvestre and probiotics in alloxan-induced diabetic rats. Methods/Case Report Methods: Experimental rats were divided into seven groups: negative control, untreated diabetic control, L. acidophilus-treated, L. casei-treated, G. sylvestre-treated, a combination of L. casei and G. sylvestre-treated, and a combination of L. acidophilus and G. sylvestre-treated. Blood glucose levels, lipid profiles, and reduced glutathione levels were measured, and histopathological examination of pancreas and kidney tissues was performed. Results (if a Case Study enter NA) Results: In the third week of the experiment, the combined administration of G. sylvestre and probiotics showed the most significant reductions in glucose levels (250 mg/dL), alkaline phosphatase (ALP) (613.3 U/L), urea (31 U/L), serum creatinine (0.77 U/L), and superoxide dismutase (SOD) (854.7 UMOL). G. sylvestre alone exhibited the lowest levels of glutamate pyruvate transaminase (GPT) (30 U/L), cholesterol (41 mg/dL), triglycerides (101.3 mg/dL), very-low-density lipoprotein (VLDL) (22.9 mg/dL), and malondialdehyde (MDA) (119.3 U MOL/SEC). Probiotic treatment alone resulted in the lowest levels of uric acid (UA) (4.4 U/L) and low-density lipoprotein (LDL) (95.06 mg/dL). G. sylvestre demonstrated the highest level of high-density lipoprotein (HDL) (44 mg/dL), while probiotics exhibited the highest catalase (CAT) activity (92 UMOL/SEC). Histopathological examination revealed the preservation of normal pancreatic and renal tissue morphology with G. sylvestre treatment. Conclusion Conclusion: The combination of G. sylvestre and probiotics enhances the antidiabetic activity compared to G. sylvestre alone in alloxan-induced diabetic rats. These findings suggest a potential synergistic effect of combining herbal and probiotic therapy for diabetes management.

&lt;em&gt;In Vitro&lt;/em&gt; antifungal activity of selected plant extracts against mango stem end rot pathogen: &lt;em&gt;Lasiodiplodia theobromae&lt;/em&gt;

Phytopathogens are primarily controlled through the application of synthetic products, which harm human and environmental health. The use of plant-derived extracts as ecofriendly bioagents has become increasingly popular due to the biologically active compounds present in the plants. Thus, a study was conducted to determine the antifungal activity of the ethanol and acetone leaf extracts of Argyreia pomacea, Coccinia grandis, Erythrina subumbrans, and Gymnema sylvestre against the causal pathogen of mango stem-end rot. Pathogen was isolated from diseased mangoes and identified as Lasiodiplodia theobromae. The pathogenicity of the isolated L. theobromae was confirmed on healthy samples of mangoes. The antifungal activity of leaf extracts was determined by the food poison technique. The leaf extracts were tested at different concentrations of 50, 25, and 12.5 mg/ml. Results indicated that the ethanol extract of E. subumbrans (29.21 ± 0.65 %) at the concentration of 50 mg/ml showed the highest percentage of mycelial growth inhibition followed by the acetone extract of G. sylvestre (28.97 ± 0.79 %) at the concentration of 25 mg/ml against the L. theobromae. The percentage of mycelial growth inhibition was significantly different among all treatments (P &lt; 0.05). This might be due to the different kinds of phytochemicals in the plant leaves. Further studies are required to isolate and identify the specific bioactive compounds from the leaves, responsible for these antifungal activities.

Comparative Effects of Gymnema sylvestre and Berberine on Adipokines, Body Composition, and Metabolic Parameters in Obese Patients: A Randomized Study.

We performed a comparative study in 50 adult Mexican patients with obesity treated with GS or BBR for 3 months. The baseline and final biochemical parameters, body composition, blood pressure, gene expression of Res, Ome, Vis, and Ap, and safety parameters were evaluated. BBR significantly decreased (p < 0.05) body weight, blood pressure and Vis and Ap gene expression and increased Ome, while GS decreased fasting glucose and Res gene expression (p < 0.05). A comparative analysis of the final measurements revealed a lower gene expression of Ap and Vis (p < 0.05) in patients treated with BBR than in those treated with GS. The most frequent adverse effects in both groups were gastrointestinal symptoms, which attenuated during the first month of treatment. In patients with obesity, BBR has a better effect on body composition, blood pressure, and the gene expression of adipokines related to metabolic risk, while GS has a better effect on fasting glucose and adipokines related to insulin resistance, with minimal side effects.

Preliminary studies of antivenom and antioxidant activities of Gymnema sylvestre R.Br. leaf extracts

Preliminary studies of antivenom and antioxidant activities of Gymnema sylvestre R.Br. leaf extracts

In vitro and in silico assessment of antidiabetic and antioxidant potencies of secondary metabolites from Gymnema sylvestre

This study investigated the chemical constituents, antioxidant potential, and in vitro and in silico antidiabetic activity of Gymnema sylvestre. Column chromatography and spectroscopic techniques identified twelve compounds from the methanol extract, including 4 sterols (1–4), 5 triterpenoids (5–9), and 3 flavonoids (10–12). The chemophenetic significance of all compounds was also investigated. The antioxidant capacity of the extract and compounds (1–4) was evaluated using FRAP and DPPH assays. The extract exhibited strong free radical scavenging activity (IC50 = 48.34 µg/mL), while compounds (1–4) displayed varying degrees of efficacy (IC50 = 98.30–286.13 µg/mL). The FRAP assay indicated significant reducing power for both extract and compounds (58.54, 47.61, 56.61, and 49.11 mg Eq.VitC/g for extract and compounds 1 &2, 3, and 4, respectively). The antidiabetic potential was assessed through α-amylase and α-glucosidase enzyme inhibition assays. The crude extract demonstrated the most potent inhibition (IC50 = 218.46 and 57.42 µg/mL for α-glucosidase and α-amylase respectively) suggesting its potential for managing postprandial hyperglycaemia. In silico studies employed molecular docking and dynamics simulations to elucidate the interactions between identified compounds and α-amylase/α-glucosidase enzymes. The results revealed promising binding affinities between the compounds and target enzymes, with compound 6 demonstrating the highest predicted inhibitory activity with −10 kcal/mol and −9.1 kcal/mol for α-amylase and α-glucosidase, respectively. This study highlights the presence of diverse bioactive compounds in Gymnema sylvestre. The extract exhibits antioxidant properties and inhibits carbohydrate-digesting enzymes, suggesting its potential as a complementary therapeutic approach for managing hyperglycaemia associated with type 2 diabetes.

Exploring the anti-diabetic mechanism of selective phytochemicals identified from Gymnema sylvestre using TLC-UPLC-MS, complemented by in silico studies

BackgroundDiabetes is a serious metabolic condition characterized by high blood glucose levels due to inadequate insulin action or insulin resistance. It can impair various metabolic pathways in cellular metabolism, leading to significant long term effects. In the Ayurvedic system of medicine, leaves of Gymnema sylvestre (GS) have been traditionally utilized to manage diabetes. Aim of the studyThis study aims is aimed to investigate the mechanism of action driven by the chemical constituents in potentially treating diabetes. MethodsMetabolite profiling of the methanolic extract from Gymnema sylvestre leaves (MLGS) was conducted using thin-layer chromatography (TLC), and ultraperformance-liquid chromatography-mass spectroscopy (UP-LCMS). Molecular Docking and MD simulations were carried out to investigate the interaction between selective phytoconstituents and the antidiabetic target, peroxisome proliferator-activated receptor (PPARγ). ResultsMLGS demonstrated significant antioxidant activity, estimated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), and with an IC50 of 103.73 μg/mL. Aditionally, MLGS also exhibited substantial inhibition of α-amylase and α-glucosidase, with an IC50 of 113.49 μg/mL and 127.40 μg/mL, respectively. Using TLC-MS-bioautography, two antioxidant metabolites and three α-amylase inhibitory metabolites were identified Quercetin was identified in both α-amylase and antioxidant prominent bands. Identified phytoconstituents, namely rutin and lupeol, exhibited favorable docking interactions with PPARγ. MD simulations results indicate that rutin creates a well-built protein-ligand complex, showed minor structural modifications, and could exhibit agonistic activity with PPARγ. This suggesting the potential of rutin as a druggable compound to treat diabetes. ConclusionThese findings suggest that MLGS is enriched with antidiabetic phytoconstituents, warranting further exploration through the isolation of antidiabetic metabolites and their testing in animal models.

Insight into the morphological and bioactive variability in the ex-situ genepool of Gymnema sylvestre accessions collected from Peninsular India

Insight into the morphological and bioactive variability in the ex-situ genepool of Gymnema sylvestre accessions collected from Peninsular India

Phytofabrication of biocompatible zinc oxide nanoparticle using Gymnema sylvestre and its potent in vitro antibacterial, antibiofilm, and cytotoxicity against human breast cancer cells (MDA-MB-231).

The increasing incidence of breast cancer and bacterial biofilm in medical devices significantly heightens global mortality and morbidity, challenging synthetic drugs. Consequently, greener-synthesized nanomaterials have emerged as a versatile alternative for various biomedical applications, offering new therapeutic avenues. This study explores the synthesis of biocompatible zinc oxide (ZnONPs) nanoparticles using Gymnema sylvestre and its antibacterial, antibiofilm, and cytotoxic properties. Characterization of ZnONPs inferred that UV-Vis spectra exhibited a sharp peak at 370nm. Fourier transform infrared spectroscopical analysis revealed the presence of active functional groups such as aldehyde, alkyne, cyclic alkene, sulfate, alkyl aryl ether, and Zn-O bonds. X-ray diffraction analysis results confirmed the crystalline nature of the nanoparticle. Scanning electron microscope analysis evidenced hexagonal morphology, and energy-dispersive X-ray analysis confirmed zinc content. High-resolution transmission electron microscope analysis showed hexagonal and rod-shaped ZnONPs with a size of 5nm. Zeta potential results affirmed the stability of nanoparticles. The ZnONPs effectively inhibited gram-positive (18-20mm) than gram-negative (12-18mm) bacterial pathogens with lower bacteriostatic and higher bactericidal values. Biofilm inhibitory property inferred ZnONPs were more effective against gram-positive (38-94%) than gram-negative bacteria (27-86%). The concentration of ZnONPs to exert 50% biofilm-inhibitory is lower against gram-positive bacteria (179.26-203.95μg/mL) than gram-negative bacteria (201.46-236.19μg/mL). Microscopic visualization inferred that at 250μg/mL, ZnONPs strongly disrupted biofilm formation, as evidenced by decreased biofilm density and altered architecture. The cytotoxicity of ZnONPs against breast cancer cells showed a dose-dependent reduction in cell viability with an IC50 value of 19.4µg/mL. AO/EB staining indicated early and late apoptotic cell death of breast cancer cells under fluorescence microscopy. The results of hemolytic activity validated the biocompatibility of the ZnONPs. Thus, the unique properties of the green-synthesized ZnONPs suggest their potential as effective drug carriers for targeted delivery in cancer therapy and the treatment of biofilm-related infections.

Formulation and Evaluation of Anti-diabetic Herbal Tablet containing Syzygium cuminii, Swertia chirata and Gymnema sylvestre

In a developing nation like India, diabetes has imposed an immense burden on the health care system. There is additionally an alpha-amylase of blood sugar levels in diabetes patients in the numerous treatment approaches. Therefore, it is unquestionably crucial to scientifically screen medicinal plants for their use in treatments to ensure that they are both safe and effective. Our present research investigates the alpha-amylase inhibitory action of methanolic extracts from the Syzygium cuminii seeds, Gymnema sylvestre leaves, and Swertia chirata leaves three indigenous medicinal herbs. With the use of the alpha-amylase model and the 3, 5-dinitro salicylic acid (DNSA) approach, the anti-diabetic action was carried out. The fundamental concept of the DNSA approach is the color-change principle, which is further validated by the colorimeter. The findings demonstrated that the alpha-amylase inhibitory action is present in all three methanolic extracts of Syzygium cuminii seeds, Gymnema sylvestre leaves, and Swertia chirata leaves. Furthermore, we concluded that Syzygium cuminii seed extract is more effective than the other two at inhibiting alpha-amylase activity. Based on the findings of this study, we endorse the use of all three methanolic extracts in the management of diabetes mellitus.

Further Studies in the Pollination of some Indian Asclepiads

Mechanism of pollination, attachment of pollinia with various body parts of insect visitors of order hymenoptera and lepidoptera and their role in pollination of a few asclepiads, viz. Asclepias curassavica L., Calotropis procera (Ait.) R. Br, Wattakaka volubilis (L.) Stapf. Leptadenia reticulata R.Br., Sarcostemma (Oxystelma) secamone (L.) Bennett. and Pergularia daemia (Forsk) Blatt. has been discussed. However, autophily by insu germination of Pollinia in three genera, viz., Hemidesmus indicus Br. Gymnema sylvestre Br. and Tylophora hirsuta (Burm f.) Merr. has been reported for the first time in family Asclepiadaceae.

A Review on “The In-vitro Study of Anticoagulant Activity of Different Plant Extract”

The aim of this review is to evaluate the plant extract which show highest anticoagulant activity. There are different plant extract to show the anticoagulant activity. On the basis of this study to know about which plant show maximum anticoagulant activity and which plant has minimum anticoagulant activity. The plants used are naturally obtained. They have other pharmacological properties but some plant have significant anticoagulant activity. The plant used for studied are Myrciaria plinioides, Terminalia bellerica, Gymnema sylvestre, Jatropa gossypiifolia, Allium sativum , Curcuma longa,Rubus ulmifolius, Gymnema sylvestre. Among these plant the Gymnema sylvestre has maximum anticoagulant activity, and the other plant show a significant anticoagulant activity. The prothrombin test, activated prothrombin test , thrombin test, recalcification method were used for the in vitro test of anticoagulant activity. The extract of plant were obtained by various extraction process such as maceration, soxhlet extraction, decoction method etc.

Exploring non-cytotoxic, antioxidant, and anti-inflammatory properties of selenium nanoparticles synthesized from Gymnema sylvestre and Cinnamon cassia extracts for herbal nanomedicine

The increasing need for pharmaceutical agents that possess attributes such as safety, cost-effectiveness, environmental sustainability, and absence of side effects has driven the advancement of nanomedicine research, which lies at the convergence of nanotechnology and medicine. Aims and objectivesThe study aimed to synthesize non-toxic selenium nanoparticles (SeNPs) using Gymnema sylvestre (G. sylvestre) and Cinnamon cassia (C. cassia) extracts. It also sought to develop and evaluate versatile nanomedicine formulations i.e. selenium nanoparticles of G. sylvestre and C. cassia (SeNPs), drug (lupeol) loaded SeNPs (DLSeNPs), drug-loaded and coated (PEG) SeNPs (DLCSeNPs) without side effects. MethodsThe SeNPs formulations were hydrothermally synthesized, loaded with lupeol to improve efficacy, coated with polyethylene glycol (PEG) for targeted delivery, and characterized using UV–Vis spectrophotometry, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), zeta potential analysis, size distribution analysis, and X-ray diffraction (XRD). Hemolytic cytotoxicity, 2,2-Diphenyl-1-picrylhydzayl (DPPH), total Reducing power, and total antioxidant capacity (TAC) antioxidant assays, carrageenan-induced paw edema, and histological studies were used to estimate the acute anti-inflammatory activity of the synthesized SeNPs. ResultsThe final form of PEGylated and drug (lupeol)-loaded selenium nanoparticles (DLCSeNPs) exhibited an average particle size ranging from 100 to 500 nm as evidenced by SEM, and Zeta potential results. These nanoparticles demonstrated no cytotoxic effects and displayed remarkable antioxidant (IC50 values 19.29) and anti-inflammatory capabilities. These results were fed into Graph-pad Prism 5 software and analyzed by one-way ANOVA, followed by Tukey's post hoc test (p < 0.001). All nano-formulations exhibited significant overall antioxidant activity, with IC50 values ≤ 386 (p < 0.05) as analyzed by ANOVA. The study's results suggest that G. sylvestre outperformed C. cassia in terms of reducing 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical, potassium ferricyanide, and ammonium molybdate in respective antioxidant assays. As far as anti-inflammatory activities are concerned drug (lupeol)-loaded and PEG-coated G. sylvestre SeNPs exhibited the highest anti-inflammatory potential from all other nano-formulations including drug (lupeol)-loaded and PEG-coated C. cassia SeNPs, as exhibited to reduce the release of pro-inflammatory signals i.e. cytokines and NF-kB, making them innovative anti-inflammatory nanomedicine. ConclusionThe study synthesized lupeol-loaded and PEG-coated SeNPs, showcasing the potential for biocompatible, cost-effective anti-inflammatory nanomedicines. G. Sylvester's superior antioxidant and anti-inflammatory performance than Cinnamon cassia emphasizes medicinal plant versatility.

In silico modelling of proteins encoded by important genes of flavonoid biosynthesis pathway in Gymnema sylvestre R. Br.

Understanding the proteins and metabolites produced by plants aids in developing superior planting materials. Gymnema sylvestre, a significant medicinal plant containing beneficial compounds such as oleanane, dammarane, flavonoids and gymnemic acids, exhibiting antioxidant and antidiabetic properties, making it a promising alternative treatment for Type 2 diabetes with minimal side effects. Transcriptomics in G. sylvestre yielded Coding DNA Sequences (CDS) for 12 key genes of flavonoid biosynthesis. Translated protein sequences of those 12 genes underwent characterization using protPARAM and i-TASSER. In silico protein modelling found similarities to validated enzymes, suggesting functional potential of the studied proteins. The study enlightens the first comprehensive insight into key proteins involved in G. sylvestre's flavonoid biosynthesis pathway. GsCHS (G. sylvestre chalcone synthase), a key enzyme in flavonoid biosynthesis, corroborated its phylogenetic proximity to Vincetoxicum mongolicum's CHS. Protein docking analysis delineated the distinctive features of GsCHS from cpCHS1 of Cyclosorus parasiticus, particularly in its substrate binding pockets and interactive sites crucial for synthesizing essential flavonoids.

Effect of Integrated Nutrient Management on Growth and Yield of Gymnema sylvestre (R.Br.), in Jharkhand (India)

Gymnema sylvestre (R.Br.) is a climber plant commonly called Gudmar belongs to family Apocynaceae is deciduous seed plant. A field experiment was conducted during 2017-2019 at the experimental field of Birsa Agricultural University, Ranchi, Jharkhand (India) to find out the integrated nutrient management technique for increasing the yield of the leaf of Gymnema sylvestre (R.Br.). The experiment was laid out in a Randomized Block Design with 12 treatments and one control replicated thrice. Nine plants were transplanted in each treatment at 80 cm x 80 cm spacing level. It is an important medicinal plant used as an herbal remedy for high blood sugar (type-II diabetes). The optimum doses of organic and inorganic fertilizer combination should be 10t ha-1 VC+10t ha-1 NC+5t ha-1 KC +30:40:40 kg NPK ha-1 for the maximum productivity of leaves and stem of Gudmar. The combination of organic and inorganic is superior to the application of fertilizer alone and controls for achieving higher growth and yield.

In vitro Inhibitory effects of Polyherbal Formulation on Biofilm-forming bacteria in Chronic wounds

Biofilm is a complex microbial community highly resistant to antimicrobials. The formation of biofilms in biotic and abiotic surfaces is associated with high rates of morbidity and mortality in hospitalized patients. The aim of the present study was to develop a polyherbal formulation for antibacterial activity and inhibition of biofilm formation. Andrographis paniculata (leaves), Cassia fistula (flower), Gymnema sylvestre (leaves), Ocimum tenuiflorforum (leaves), Cinnamomum verum (bark)were the herbs chosen for the study. The extraction of the herbal samples was carried out using the cold extraction method and their phytochemical characterization was carried out by GC-MS analysis. The anti-microbial activity and antibiofilm activity of polyherbal formulation was carried out using the agar well diffusion method and microtitre plate assay. The phytochemical results showed the presence of resins, carbohydrates, proteins, saponin and phenol. The agar well diffusion assay results and microtitre plate assay showed the highest activity exhibited with 500µg/ml of polyherbal extract against Pseudomonas aeruginosa and lowest against Escherichia coli. The results confirm the differences in antibacterial and antibiofilm activity of polyherbal formulation of various concentration. Larger concentration of formulation showed to be more effective against biofilm cells. The purpose of this study was to investigate the anti-biofilm properties and inhibitory impact of ACGOC extracts against the most prevalent clinical isolates such as S. aureus,B.pumilusand P. aeruginosa and so the hypothesis of synergistic interaction of biologically active compounds from five different herbal extracts was proved for its antibiofilm assays.