Gutta-percha Research Articles

Comparison of Apical Sealing Capacity of ActiV GP/Glass Ionomer Sealer Versus Resilon/RealSeal and Gutta Percha/AH plus Sealers.

In recent years, concerns have arisen regarding the sealing efficacy of traditional root canal obturation materials such as gutta-percha (GP) and various sealers. The resin-based obturation complexes like RealSealTM (SybronEndo, Orange, CA) and ResilonTM(ResilonResearch LLC, Madison, CT) had been developed as replacements for conventional obturation materials, gutta-percha, and various types of sealers. Additionally, ActiV GPTM (Brasseler USA, Savannah, GA), a glass-ionomer-based obturation system, has gained attention. This in vitro study's objective was to evaluate ActiV GPTM/GI (glass ionomer) sealer's sealing capacity and compare it to that of ResilonTM/RealSealTM as well as gutta-percha/AH PlusTM (Dentsply International Inc, York, PA) takenas a control. In this in vitro investigation, 90 freshly excised single-rooted humanpremolars of the mandible were chosen. According to the substance used to seal the root canals, the teethwere randomly separated into three separate groups (n=30). Group 1: The ActiV GPTM obturation system was used to seal and obturate the canals. Group 2: ResilonTM/RealSealTMwas used to obturate and block the canals. Group 3: GPpoints and AH PlusTM were used to seal the canals employing the lateral condensation methodology. All of the root surfaces-aside from the last 2 mm of the root-were painted with two coats of nail polish before being submerged in 2%methylene blue for a duration of 24 hours at a temperature of37°C. The highest amount of dye leakage was determined using a stereomicroscope at 20X magnification. The extent of dye penetration at the apical region in specimens of the ActiV GPTM category was 4.93±1.48 mm. The depth of dye penetration at the apical region in specimens of the ResilonTM category was 2.78 ±1.62 mm. The extension of penetration of dye was 0.48± 0.46 mm in specimens of the GP/AH PlusTM category. The degree of microlikeage was maximum in ActiV GPTM specimens and it was minimum in GP/AH PlusTM specimens. The microlikeage in specimens of the Resilon category was greater than GP/AH plusTM while it was lower than ActiV GPTM specimens. The observations were statistically meaningful (p<0.001). Based on the results of the dye penetration examination, it can be concluded that the root canal wall sealing capabilities of ActiV GPTM with GI Sealer were inferior to those of GP/AH PlusTM and ResilonTM with RealSealTM.

Effect of Different Obturation Techniques on the Amount of Debris Extrusion During Endodontic Retreatment Using XP Endo Retreatment Set Files (In vitro Study)

Objective: This study aimed to assess the effect of the cold and warm obturation techniques on the amount of apically extruded debris (AED) and time needed for the removal of filling material during endodontic retreatment using the XP endo retreatment files.&#x0D; Methods: Sixty single-rooted lower premolars with round-shaped canals were selected for the study. The canals were prepared and obturated with one of four obturation techniques (N=15): cold lateral compaction (CLC), single-cone (SC), thermoplasticized gutta percha injection (TGP), and gutta core (GC) obturation techniques. All the root canal fillings were removed using XP endodontic retreatment files. The mean weight of the AED was estimated with an analytical balance to an accuracy of 10-4 g. AED data were subjected to Kruskal-Wallis and a one-way ANOVA, post hoc Tukey's test for the time factor, with a significance level ≤ 0.05.&#x0D; Results: AED data revealed significant differences among groups (p=0.039). The CLC technique was significant compared to TGP, with a significance level of p=0.018, and significant compared to the SC technique, with a significance level of p=0.034. Also, there were highly significant differences between groups regarding the time needed for retreatment (p=0.000).&#x0D; Conclusions: The obturation technique influences AED, and time for root canal retreatment. CLC resulted in less debris extrusion, while SC resulted in higher debris extrusion and TGP required the least time for root canal retreatment.

Influence of bioceramic cones on the quality of root canal filling relative to bond strength and adaptation of the adhesive interface.

To evaluate the bond strength (BS) and analysis of the adhesive interface in root canals filled with bioceramic gutta percha sealers and cones. Ninety-six maxillary canines were divided into eight groups according to the endodontic sealer (AH Plus, AH Plus Bioceramic, Bio-C Sealer or Bio-C Sealer Ion+ and gutta percha cones (conventional or bioceramic) tested. They were analyzed using the BS test, failure pattern, analysis of the adhesive interface by scanning electron microscopy and confocal laser scanning microscopy. The BS data were compared between groups using the analysis of variance test with the Turkey post-test. The chi-square test was used to assess the type of failure and the non-parametric Mann-Whitney and Kruse-Wallis tests (P < 0.05). Analysis of variance showed higher BS values for the groups of bioceramic gutta percha cones in Bio-C Sealer Ion+ (8.38 ± 4.27), AH Plus Bioceramic (6.19 ± 3.28), Bio-C Sealer (5.70 ± 3.18), AH Plus (4.61 ± 2.11) and for conventional gutta percha cones in AH Plus sealers (4.26 ± 2.35), Bio-C Sealer Ion + (3.63 ± 2.29), Bio-C Sealer (2.94 ± 2.32) and AH Plus Bioceramic (1.19 ± 0.89) (P < 0.05). Relative to the type of failure and adaptation of the types of filling material, a higher percentage of mixed failures was observed (gaps between 1µm-10µm) for the group with bioceramic gutta percha cones (P < 0.001). The bond between sealers and bioceramic gutta percha cones showed higher bond strength values and greater penetration into the dentin tubules. The filling the root canal system with bioceramic sealers should be associated with bioceramic gutta percha cones.

Aloe vera in dentistry: current status and future prospects

This review declares the chemical composition, pharmacological actions, clinical applications, adverse reactions and future prospects of Aloe vera (AV) in the field of dentistry. Literature pertinent to Aloe vera in dental practice was explored in databases like PubMed, Scopus and Google Scholar. The relevant literature was reviewed and critically appraised in this review. Aloe vera is a miracle plant that has been applied as herbal therapy for thousands of years due to its multiple health benefits. In dental practice, AV has different applications and it attracts the attention of researchers due to its natural origin, low cytotoxicity, and various biological actions like anti-microbial, immunomodulatory, anti-inflammatory, antioxidant, wound healing, and antineoplastic actions. Therefore, it has been used for treatment of oral lesions, endodontic lesions, periodontal disease, oral wounds, and alveolar osteitis as well as in tissue engineering, reduction of plaque formation, decontamination of Gutta percha, around dental implants and others. AV has been used in treatment and prevention of several other oral and dental problems. Nevertheless, it has a lot to offer in the dental practice and future studies are recommended to maximize its beneficial role in the field of dentistry.

Quality of post and core placement by final year undergraduate dental students.

The success of endodontic restoration of badly compromised teeth depends on the quality of post and core placement and the extra-coronal restoration. Ensuring that students place posts to acceptable quality standards is therefore essential. The aim of this study was to radiographically evaluate post placement by final year undergraduate dental students and to identify any predictors of performance. Two hundred retrospectively and randomly selected posts placed by sixth year students were evaluated radiographically. Data on student gender; number and quality of radiographs; periapical pathology; tooth location; root canal treatment quality; amount of remaining gutta percha; gap between gutta percha and post; post-to-root width; crown-to-root ratio; and types of core material and crowns were recorded. Four criteria were used to grade post placement quality: (i) amount of remaining gutta percha; (ii) gap between gutta percha and post; (iii) post width to root ratio; and (iv) crown-to-root ratio. Assessments were scored to produce final grades. Data are presented using descriptive statistics and the chi-squared test was used to investigate whether student gender or tooth location were associated with final grade. Post and core quality was acceptable in most cases (97.5% were graded as adequate), with no differences in quality between male and female students nor according to tooth location (anterior, premolar, and molar). Just over half of radiographs were adequate quality (53.5%), while just under half were assessed as less than adequate (46.5%) due to cone cutting, overlap, shortening or elongation, although this did not affect formal assessment of post quality. General outcomes of post and core placement by undergraduate students were good, with few errors that might affect the prognosis and long-term survival of treated teeth. Providing undergraduate dental students with clear guidelines on when and how to take radiographs throughout the procedure may improve the quality of post and core treatment and reduce the risk of multiple unnecessary radiographic exposures. From the clinical perspective, although dental students generally place high quality posts and cores, it remains important to monitor the quality and performance of post placement as this determines the survival of compromised teeth.

Comparing the softening effect of three gutta-percha solvents on different types of gutta-percha with different application durations

IntroductionDuring non-surgical endodontic retreatment, gutta-percha (GP) solvents are indispensable in difficult cases when used with mechanical removal, however studies comparing their efficacy against different types of GP are limited. The purpose of this study was to investigate the softening effect of three solvents on the conventional (CGP), cross-linked carrier-based (CLGP), and thermoplasticized (TGP) and compare the effect of time on the softening effect of the solvents. MethodsTested GP were embedded in cuboidal blocks of stone with their upper surfaces exposed (1 mm diameter). Three commercial GP solvents based on D-Limonene (DL), Eucalyptol oil (EO), and orange oil (OO) were added to the exposed GP before an indenter (weight = 1Kg) was applied. Using a digital camera, the indentation depth was measured (mm) directly after applying the solvent and indenter (T = 0), and after 1, 2, and 3 min of application (T = 1,2,3). The means of indentation depth were calculated and compared using a two-way analysis of variance and Tukey’s post-hoc test to assess the effect of the types of solvent, GP for each application duration, and Friedman’s test to evaluate the effect of application duration on the softening effect of solvents. ResultsThe type of GP (F = 261.43, p < 0.001), type of solvent (F = 3.57, p = 0.015), and application duration (F = 53.088, p < 0.001) were all found to significantly affect GP softening. DL exhibited the highest and only significant effect on CGP after 1 min (p < 0.05), while OO had the only significant effect against CLGP when applied for at least 2 min (p < 0.05). Both OO and EO had significant softening effects on TGP instantly or after 1 min of application, respectively. ConclusionsThe results of this study revealed that the softening effect of GP solvents varies depending on their type, their application duration, and the type of GP.

Evaluation of the efficacy of the Lentulo spiral filler operated at four different speeds and with two different techniques in cadaveric canine teeth of dogs.

This study evaluated the effect of filling technique and rotational speed on the efficacy of sealer cement placement using the Lentulo spiral filler. Cadaveric maxillary and mandibular canine teeth (n = 74) from age and breed matched fresh-frozen adult canine cadaver heads were utilized. Following routine mechano-chemical preparation, teeth were randomly divided into 8 treatment groups with varying rotational speeds [250-2,000 revolutions per minute (RPM)] and fill techniques (backfill technique versus pumping technique). The quality of the sealer cement fill was evaluated radiographically before and after master gutta percha cone placement. Percentage of voids present in the apical, midbody, and coronal portions of the tooth were subjectively scored as: no voids present, voids present in less than 25% of the area, voids present in 25-50% of the area, voids present in greater than 50% of the area. The apex was also scored as underfilled, adequately filled, or overfilled. Operating the Lentulo spiral filler at 2,000 RPM resulted in the best quality fill regardless of filling technique. The pumping technique with placement of the master gutta percha cone had a higher probability of success as compared to the backfill technique, but this finding lacked significance. Regardless of speed or technique, the apical region had the highest quality of fill. The addition of the master gutta percha cone improved the quality of fill. Therefore, we recommend using the Lentulo spiral filler at higher speeds with a pumping technique (followed by addition of a master gutta percha cone) to improve the quality of epoxy resin-based sealer cement placement.

Evaluation of Different Techniques and Materials for Filling in 3-dimensional Printed Teeth Replicas with Perforating Internal Resorption by Means of Micro–Computed Tomography

IntroductionThe aim of this study was to evaluate the filling ability of 2 obturation techniques in 3-dimensional (3D) printed teeth with perforating internal resorption (PIR). MethodsA maxillary central incisor was instrumented and scanned by micro–computed tomographic (micro-CT) imaging. The 3D model was exported in the stereolithographic format and, with the aid of OrtogOnBlender software (Cícero Moraes, Sinop, SP, Brazil), a PIR in the middle third of the root canal was designed. Thirty-two replicas were printed in surgical resin and distributed into 4 groups (n = 8) according to the obturation technique and the material used: 2 groups used the hybrid technique, 1 with Bio-C Sealer (BCS; Angelus, Londrina, PR, Brazil)/gutta-percha (GP; VDW GmbH, Munich, Germany) + Bio-C Repair (BCR; Angelus, Londrina, PR, Brazil) and the other with BioRoot (BR; Septodont, Saint Maur des Fosses, France)/gutta-percha (GP) + Biodentine (BD; Septodont, Saint Maur des Fosses, France), and 2 groups used the incremental technique, 1 with BCR and the other with BD. Postobturation micro-CT imaging was performed to measure the percentage volume of voids and laser confocal microscopy to measure the surface roughness (μm) of the repair cements. Data were compared using analysis of variance and Kruskal-Wallis tests. ResultsRegarding the filling volume in the apical third, the BCS/GP + BCR (89.70 ± 5.15), BR/GP + BD (87.70 ± 8.43), and BCR (84.20 ± 9.00) groups showed the highest percentages compared with the BD group (69.70 ± 6.88) (P < .05). In the area of internal resorption, the BCS/GP + BCR (96.00 ± 2.64) and BCR (95.30 ± 2.93) groups showed the highest percentages compared with the BR/GP + BD group (91.50 ± 1.35) (P < .05). The BD group showed intermediate values that were sometimes similar to the BCS/GP + BCR and BCR groups and similar to the BR/GP + BD group (P > .05). Regarding the quality of the filling in the perforation area, the BCR group showed better results compared with the BD group (P < .001). Regarding roughness, the BCR group (1.66 ± 0.65) showed lower surface roughness compared with the BD group (2.51 ± 0.89) (P < .05). ConclusionsThe capacity and quality of the filling in teeth with PIR were superior with the incremental technique with BCR and the hybrid technique with BCS/GP + BCR.

Influence of heat treated files on conserving the remaining dentin thickness during endodontic retreatment – An invitro CBCT study

ObjectivesNon-surgical endodontic retreatment is to be a conservative option over endodontic surgery in case of recurrent infections. Root canal retreatment procedures needs to be initiated faster and proficiently with proper root canal retreatment files. The main aim of this study is to evaluate the remaining root dentin thickness post Gutta Percha (GP) retrieval from the root canal after using two different rotary instruments. Materials and methodsA total of sixty extracted single rooted teeth were chosen. Shaping and Cleaning was done Step Back (Telescopic) technique with apical preparation ofsize 40 k File. Irrigation was carried out using NaOCl (3 %)and Ethyelenediamine tetraacetic acid (EDTA) to clear the smear layer. The canals were dried using paper points and obturation was completed using lateral compaction technique with AH plus resin sealer. Upon completing the obturation, it was categorized into two groups: Group I- ProTaper retreatment files (PTR) and Group II- Solite RS3(S-RS3) Retreatment files. Cone Beam Computed Tomography (CBCT) was used to assess the remaining dentin thickness post gutta-percha retrieval at 3,5 and 7 mm respectively. To test the significance between groups independent t test was used. ResultsIt can be noted from the results that more amount of remaining dentin thickness was seen after using Solite RS3 files at 3 mm, 5 mm and 7 mm on the mesial side compared to ProTaper retreatment files (p < 0.05). There is no statistically significant difference seen between the files at three levels on the distal side (p > 0.05). ConclusionWithin the limitations of the study, it can be concluded that Solite RS3 files have the potential to preserve the remaining dentin thickness. However, more studies including various other parameters should be performed to arrive at a definitive conclusion.

Comparative evaluation of the effectiveness of various solvents on dissolving efficacy of gutta percha- An in vitro- study

This study's objective was to compare how well xylene, chloroform, turpentine oil, halothane, and orange oil dissolved materials at three different immersion time intervals.For the investigation. An ISO no. 40 cone and gutta-percha cones with a 0.06 taper were gathered. In each of the tested solvents, five batches of samples were made and submerged for 6, 12, and 18 minutes. To ascertain the process of gutta-percha dissolution in the solvents, the weight before immersion and the weight after immersion in the solvents were measured on a digital analytical scale. Analysis of Variance (ANOVA) and multiple comparisons with Scheffe's test (p 0.05) were used to statistically analyse the data. With xylene, the best solvency capacity was attained. Halothane, orange oil, and chloroform all had comparable dissolving abilities.

Effect of silver diamine fluoride activation on bond strength to root dentin

BackgroundTo investigate the effect of silver diamine fluoride (SDF) application and activation on the bond strength of gutta-percha to dentin and resin bonded post to dentin.MethodsThirty-six human premolar teeth were used. The coronal part tooth was removed, and endodontic mechanical preparation was performed for all the teeth. The teeth were divided according to final rinse protocol (n = 9) as follows: Control group; no SDF application, SDF/NA; 38% SDF as a final rinse with no activation, SDF/MDA; 38% SDF as a final rinse with manual dynamic activation and SDF/US; 38% SDF as a final rinse with ultrasonic activation. Root canal obturation using lateral condensation technique followed by fiber post insertion after 48 h in the root canal after corresponding preparations. The roots were cut with a low-speed precision saw creating 2 mm thickness sections. A total of 4 sections were obtained from each tooth, 2 coronal specimens (with post) and 2 apical specimens (with Gutta percha). Each specimen was subjected to push-out bond strength test with a universal testing machine. Data were analyzed using two-way ANOVA.ResultsThe Push-out bond strength at the apical root section was significantly higher in SDF/MDA and SDF/US groups compared to control group. While for the coronal part, all SDF treated specimen showed reduced Push-out bond strength.ConclusionSDF application as final rinse may reduce the bonding performance between fiber post and dentine. Activation with manual and ultrasonic methods improved the bond strength at the apical root section.

Comparison of root end sealing ability of three retrograde filling materials in teeth with root apices resected at 900 using dye penetration method under fluorescent microscope

Background: The main factor contributing to surgical endodontic dissatisfaction is an inadequate seal at the apex. The material used for retrograde filling should prevent contaminants from penetrating periradicular tissue. So the objective of this study was to evaluate and compare the ability of Mineral Trioxide Aggregate (MTA), Zirconomer and bioactive bone cement to seal the root end as retrograde filling material with apices resected at 900 angles using dye penetration method under fluorescent microscope. Methods: 36 extracted teeth samples were cut at Cementoenamel junction horizontally. Following the customary protocols of, cleaning, shaping and obturation with gutta percha and AH Plus sealer, resection of samples was done 3mm at the apical end at 90 degree angle along the long axis of the tooth with the help of diamond disc. A depth of 3mm of root end cavity was made with diamond coated ultrasonic surgical tip. The samples were randomly allocated in 3 groups: Group I: MTA ; Group II : Zirconomer; Group III: Bioactive Bone Cement . Coating of nail varnish was done with the roots except at the tip. Each material was compacted in the retrocavity using small pluggers. All the retrofilled samples were kept in acridine orange for 24 hours, following which cleaning &amp; bucco-lingual sectioning (vertical) was carried out. Confocal laser scanning fluorescent microscope was used for checking the dye penetration in these samples thereby evaluating sealing ability. Results: Group III (Bioactive Bone Cement) had the highest root end sealing ability with mean (1.4 ± 0.51) followed by Group I (MTA) (2.3 ± 0.65) and lastly Group II (Zirconomer) (4.1 ± 0.57) as root end filling material with apices resected at 900 angle using dye penetration method under fluorescent microscopy which were statistically significant. Conclusions: Bioactive Bone cement can be recommended as an acceptable retrograde filling material.

External Root Surface Temperature Control with 1,1,1,2-Tetrafluoroethane Intracanal Cryotherapy during Thermoplastic Obturation: An In Vitro Study.

The aim of this in vitro study was to determine the effectiveness of 1,1,1,2-tetrafluoroethane (TFE) intracanal cryotherapy for external root surface temperature control during thermoplastic obturation. Thirty extracted adult single-rooted mandibular incisors were selected for this study. Endodontic shaping was performed until size X3 Protaper Next Rotary endodontic file. The teeth were divided into three groups: Group I-Control group wherein conventional irrigation was done using physiologic saline stored at room temperature, Group II-Irrigational Cryotherapy group using physiologic saline at 2.5°C, and Group III-Intracanal TFE cryotherapy group with intracanal refrigerant TFE application. Temperatures were recorded in the apical 3 mm before and after completion of each intervention and post thermoplastic obturation using a noncontact digital laser infrared thermometer. Intracanal cryotherapy with TFE resulted in a mean decrease of 9.27°C compared with conventional irrigation that exhibited in a mean decrease of 2.13°C. Also, in intracanal cryotherapy group with TFE application, compared with the baseline (24.50°C), no significant differences were observed post obturation (24.61°C) with high-temperature-injectable gutta percha technique indicating good control of temperature rise on the external root surface. Intracanal cryotherapy with refrigerant TFE was highly effective in controlling temperature rise on the external root surface during injectable thermoplastic obturation technique. Minimizing deleterious effects due to high temperatures generated during the thermoplastic obturation is critical. Clinically feasible measures to reduce the transmission of heat generated during thermoplastic obturation have been searched since long. In this regard, intracanal cryotherapy with TFE can be effectively used to control the rise of temperature on the external root surface when employing thermoplastic obturation technique.

The Effect of Patency Files on Apical Canal Anatomy Using SEM Imaging.

There are many reasons to maintain apical patency during routine endodontic treatment. Thousands of canals are treated utilizing a patency file every year all around the world. The effect patency has on the apical anatomy of the root has been controversial for generations. This ex vivo descriptive study was created to show the effect patency files actually have on the apical root canal anatomy using visually detailed SEM images supported by dental radiographs. Three extracted maxillary anterior teeth that represent the multitude of canals in vivo were instrumented utilizing patency files. Two of the three maxillary anterior teeth were instrumented with hand files, the other maxillary anterior tooth with a .06 taper rotary file. The teeth were then scanned with an electron microscope to view the effect that the instruments had on the apical canal anatomy. A fourth tooth, a maxillary second molar, was shaped with an .06 taper rotary file and cone fitted. This tooth was radiographed with a gutta percha cone fitted to reveal the position of the narrowest constriction after patency was achieved. The patency files, both hand files and rotary, were shown not to adversely affect the apical canal anatomy. Additionally, the SEM's revealed a precise demarcation of cementum to dentin which was at the root surface after patency was achieved. The patent use of greater tapered rotary files provides a clear demarcation of the CDJ which allows a precise acquisition of the narrowest constriction of the canal with the use of an electronic apex locator for establishing the ideal working length and precision placement of a gutta percha cone.

Radiographical assessment of post and core placement errors encountered by Saudi dental students at different educational levels

Background: Dental post and core is one of the common procedures performed after endodontic treatment. The aim of this study was to radiographically assess the quality of post and core procedures performed by dental students at different education levels in addition to determining the most critical errors encountered during their clinical practice. Methods: A retrospective cross-sectional study design was conducted in the College of Dentistry, Imam Abdulrahman Bin Faisal University. A total of 550 periapical radiographs (PAs) of cemented posts were retrieved from the records of patients treated by dental students. Parameters and guidelines for assessing the quality of post treatment have been determined and statistically analyzed. A P value &lt;0.05 was considered statistically significant. Results: The study included 502 students and most of them were females (66.5%). Data were obtained from 502 patients (62% females) with fiber posts used in 98.2% of the cases. About 50% of the posts were inserted in premolars, 62.9% in the upper arch, and 66.7% were restored with crowns as a final restoration. Regarding the quality of posts, 98.4% showed good preparation quality and 98% showed good radiographic quality. The post diameter was equal to 1/3 of the root diameter in 31.9% of the cases; post length was equal to 2/3 of root length in 5% of the cases and equal to or more than crown height in all cases (100%). Length of the remaining gutta percha (GP) was between 3–5 mm in 38.8%, and there was no gap between the post and remaining GP in 95.6% of the cases. There were no statistically significant differences between dental students at different clinical educational levels regarding the quality of post placement. Conclusions: The quality of post and core procedures performed by students showed acceptable radiographic quality and were within the recommended standards.

Novel Electrospun Polymeric Nanofibers Loaded Different Medicaments as Drug Delivery Systems for Regenerative Endodontics.

A combination of antibiotics, including metronidazole (MET), ciprofloxacin (CIP), and minocycline (MINO), has been demonstrated to disinfect bacteria in necrotic teeth before regenerative processes. It has been presented clinically that antibiotic pastes may drive to possible stem cell death, creating difficulties in removing from the canal system, which can limit the regenerative procedure. This study was designed to (1) synthesize nanofibrous webs containing various concentrations of different medicaments (triple, double, and calcium hydroxide, Ca(OH)2), and (2) coat the electrospun fibrous gutta-percha (GP) cones. Poly(vinylpyrrolidone) (PVP)-based electrospun fibrous webs were processed with low medicament concentrations. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS) were carried out to investigate fiber morphology and antibiotic incorporation, and characterize GP-coated fibrous webs, respectively. The chemical and physical properties of dentine were determined via fourier transform infrared spectroscopy (FTIR) and Nano-SEM, respectively. The antimicrobial properties of the different fibrous webs were assessed against various bacteria by direct nanofiber/bacteria contact. Cytocompatibility was measured by applying the MTT method. The mean fiber diameter of the experimental groups of medicament-containing fibers ranged in the nm scale and was significantly smaller than PVP fibers. EDX analysis confirmed the presence of medicaments in the nanofibers. XPS analysis presented a complete coating of the fibers with GPs; FTIR and Nano-SEM showed no chemical and physical configuration of intracanal medicaments on the dentine surface. Meanwhile, nanofibrous webs led to a significant reduction in the percentage of viable bacteria compared to the negative control and PVP. Our findings suggest that TA-NFs, DA-NFs, and Ca(OH)2)-NFs coated GP cones have significant potential in eliminating intracanal bacteria, having cell-friendly behavior and clinical usage features.

Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay.

The practice of removing root canal fillings with solvent materials is frequently required to help an irrigation solution enter the tubules. The current research was aimed at assessing the antifungal properties specifically the candida albicans of the various solvent materials used for the gutta-percha (GP) material. Current research was aimed at as a lab method using the disk diffusion technique where the zone of inhibition (ZOI) was calculated. The materials that were analyzed were: orange oil, xylene, turpentine oil, chloroform, and eucalyptus oil. Candida albicans was the test organism employed in the investigation. The agar plates were covered with approximately 500 μL of the suspension. The sterile and empty disks were impregnated with 10 μL of pure GP solvents. These plates were incubated for one day at room temperature. The ZOI's mean diameters were calculated for all five materials and quantified each solvent's fungicidal activity. For intergroup comparison, ANOVA was utilized. P values < 0.05 were deemed substantial. The maximum inhibition exhibited by the Eucalyptus Oil it was 19.01 ± 1.02 mm. This was followed by Xylene. The other three solvents Chloroform, Orange Oil, and Turpentine Oil exhibited a similar ZOI. When all the solvents were compared there was a significant variance of P < 0.001. However, there were significant variances for the Eucalyptus Oil and the Xylene to all the other solvents P < 0.001. This investigation showed that, in comparison to other solvents, the use of eucalyptus oil considerably reduced the levels of Candida Albicans.

Analysis of porosity, sealer dissolution and apical extrusion of endodontic sealers: A micro computed tomography study.

The aim of the study was to determine the porosity, dissolution and apical extrusion of AH Plus, MTA Fillapex and EndoSequence BC sealer after immersion in phosphate buffered saline (PBS) using microcomputed tomography (micro-CT). Forty-eight single-rooted teeth were selected. Gutta percha and one of the aforementioned root canal sealers was used for obturation using continuous wave technique. The specimens were scanned after obturation and after seven days of immersion in PBS, by using micro-computed tomography. Porosity, sealer dissolution and apical extrusion were calculated. Statistical analysis was done by using paired t-test, post hoc tukey test and Fischer exact test. Significantly higher percentage of porosity and dissolution of sealer in the apical 4mm was found for MTA Fillapex and EndoSequence BC sealer than for AH Plus. The incidence of apical extrusion was significantly more in MTA Fillapex (56.25%), followed by EndoSequence BC (31.25%) and AH Plus (0%). None of the three root canal sealers exhibited perfect three-dimensional obturation. The sealers manifested porosity, dissolution and apical extrusion to varying degrees both after obturation and after storage in PBS for 7 days.

Potensi Aloe vera sebagai Bahan Disinfeksi Kon Gutaperca sebelum Pengisian Saluran Akar

Background: Obturation of the root canal aims to close access from the coronal and from the apical into the root canal. Currently the most widely used root canal filling material is guttapercha, due to its plastic properties, easy to manipulate, and easy to remove if necessary. Before use, guttapercha should be disinfected first to avoid re-infection of the root canal. One of the herbal plants that can be used as a guttaperca disinfectant is the Aloe vera plant because it contains phytochemical compounds that are antibacterial. Objective: To explain the potency of the herbal plant Aloe vera as a guttaperca disinfectant before root canal filling. Method: Collecting references in the form of research reports and literature studies as well as textbooks containing theories related to writing titles accessed through the Google Scholar, Research Gate, and NCBI databases published from 2012–2021. Conclusion: Aloe vera has the potential as an alternative material for disinfecting gutta percha because it has a fairly good antibacterial effect with very minimal side effects, so that Aloe vera can be a promising disinfectant in the future.

Quantitative Assessment of Dimensional Evaluation and Artefacts from Filling Materials with CBCT Using Standard Phantom Roots.

To investigate the accuracy of dimensional evaluation and representation of artefacts generated by different gutta-percha (GP) cones with or without sealer with CBCT using a reproducible, standardised phantom root methodology. The reproducible artificial phantom roots with six root canal sizes from #25 to #50 and 0.04 taper were aligned according to the jaw curvature in a stone model for dimensional measurements. Each root was scanned while empty and filled with four types of filling materials. The specimens were scanned using the CS 9300 3D (Carestream Dental, Rochester, NY, USA) (at two different resolutions), 3D Accuitomo (J Morita, Kyoto, Japan) and NewTom VGi (Verona, Italy) CBCT systems. The hyperdense and hypodense axial slice artefacts from root canal sizes #40, #45 and #50 were recorded. Dimensions were significantly smaller and more accurate with CS 9300/0.09 mm voxel size than with other protocols. The hypodense band was found mostly in the CS 9300 3D system with 0.18 mm voxel size, especially in the buccal-lingual (95%) and coronal (64%) sections. The 3D Accuitomo CBCT system showed the lowest presence of the hypodense band. Areas of both light and dark artefacts were significantly larger in the coronal third than in the apical and middle thirds. Artefacts in the coronal locations and in buccal-lingual sections were more evident in the CS 9300 3D system with a 0.18-mm voxel size.