This paper describes an experimental apparatus and procedure for the calibration of the ultrasonic lubricant-film thickness measurement technique. It also presents a study of the accuracy of the technique. The calibration apparatus is demonstrated on a three layer steel–mineral oil–steel system. This was chosen to be representative of a typical bearing system which is the industrial application of the technique. In such bearing systems the lubricant-film thickness typically ranges from 0.1 to 100 µm. The calibration apparatus uses a high precision piezoelectric displacement translator to controllably displace one of the steel surfaces relative to the other and hence alter the lubricant-film thickness by a known amount. Through-thickness resonant frequency measurements are then used to accurately measure a thick lubricant film (h > 10 µm). These resonant frequency measurements form the starting point of the calibration. The displacement translator is then used to reduce the lubricant-film thickness into the, more practically interesting, low micron range. In this range the amplitude of the measured reflection coefficient is used via a spring interface model to calculate the lubricant-film thickness. Issues of ultrasonic beam alignment and frequency of operation are discussed. A detailed study of the effect of reflection-coefficient errors on the resultant thickness measurement is presented. Practical guidelines for use of the calibration are then defined and calibration is demonstrated experimentally over the range 0.5–1.3 µm.