Ontogeny Research Articles

Cottonseed meal protein hydrolysate influences growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology in yellow-feather broilers.

This study investigated the effects of cottonseed meal protein hydrolysate (CPH) on the growth performance, carcass characteristics, serum biochemical indices, intestinal morphology, and enzyme activities of yellow-feather broilers. We randomly divided 240 chicks into four groups, each with six replicates: a basal diet with 0% (CON), 1% (LCPH), 3% (MCPH), or 5% (HCPH) CPH. The trail spanned 63 days and included three phases: Days 1-21, 22-42, and 43-63. Increased average daily gain (ADG) and decreased ratio of feed to gain (F/G) with LCPH were observed in 21-day-old broilers (P < 0.05). MCPH led to higher ADG and average daily feed intake (ADFI) in 42-day-old broilers (P < 0.05). Additionally, CPH supplementation resulted in increased dressing percentage, percentage of half-eviscerated yield, percentage of eviscerated yield, breast muscle rate, and leg muscle rate were observed (P < 0.05) with diet. The serum levels of total protein (TP), high-density lipoprotein cholesterol (HDL-C), calcium (Ca), and phosphorus (P) were enhanced, and blood urea nitrogen (BUN) and triglyceride (TG) levels decreased with diet and CPH (P < 0.05). CPH increased the length of the jejunum and ileum and the weight of the duodenum, jejunum, and ileum in 21-day-old broilers (P < 0.05). Alterations in the duodenal villus structure in broilers occurred on Days 21 and 42, and the CPH groups performed better; however, a similar change occurred in the jejunum on Days 42 and 63 (P < 0.05). MCPH and HCPH enhanced trypsin activity in the duodenum of 21-day-old and 63-day-old broilers (p < 0.05). Chymotrypsin activity increased (P > 0.05) in the duodenum of 63-day-old broilers fed MCPH. Lipase activity increased (P < 0.05) in the jejuna of 21-day-old broilers treated with HCPH. CPH increased trypsin activity in the ilea of 21-day-old broilers (P < 0.05). These results showed that CPH influenced the growth performance, carcass characteristics, serum biochemical indices, and intestinal morphology of yellow-feather broilers, which are related to growth stage. The recommended CPH level in broilers is 1% before 21 days of age and 3% after 21 days of age.

Microsatellite marker-assisted backcross breeding for improvement of wheat salt tolerance using Kharchia 65

BackgroundSalinity is a significant abiotic stress that affects plants from germination through all growth stages. This study was aimed to determine the morpho-physiological and genetic variations in BC1F2, BC2F1 and F3 generations resulting from the cross combination WH1105 × Kharchia 65.ResultsA significant reduction in germination percentage was observed under salt stress in BC1F2 and F3 seeds. Correlation, heritability in the broad sense, phenotypic coefficient of variability (PCV) and genotypic coefficient of variability (GCV) were measured for all traits. The presence of both Nax1 and Nax2 loci was confirmed in twenty-nine plants using the marker-assisted selection technique. Genetic relationships among the populations were assessed using twenty-four polymorphic SSR markers.ConclusionCluster analysis along with two and three-dimensional PCA scaling (Principal Component Analysis) revealed the distinct nature of WH 1105 and Kharchia 65. Six plants closer to the recurrent parent (WH1105) selected through this study can serve as valuable genetic material for salt-tolerant wheat improvement programs.

Straw Returning Proves Advantageous for Regulating Water and Salt Levels, Facilitating Nutrient Accumulation, and Promoting Crop Growth in Coastal Saline Soils

Saline soils limit plant growth due to high salinity. Straw returning has proven effective in enhancing soil adaptability and agricultural stability on saline lands. This study evaluates the effects of different straw-returning methods—straw mulching (SM), straw incorporation (SI), and straw biochar (BC)—on soil nutrients, water dynamics, and salinity in a barley–cotton rotation system using field box experiments. SM improved soil water retention during barley’s jointing and heading stages, while SI was more effective in its filling and maturation stages. BC showed lesser water storage capacity. During cotton’s growth, SI enhanced early-stage water retention, and SM benefited the flowering and boll opening stages. Grey relational analysis pinpointed significant water relationships at 10 cm and 20 cm soil depths, with SM regulating water across layers. SM and BC notably reduced soil conductivity, primarily within the top 20 cm, and their effectiveness decreased with depth. SI significantly lowered soil conductivity at barley’s jointing stage. SM effectively reduced salinity at 10 cm and 20 cm soil depths, whereas BC decreased soil conductivity throughout barley’s jointing, filling, and heading stages. For cotton, SI lowered soil conductivity at the seedling and boll opening stages. SM consistently reduced salinity across all stages, and BC decreased conductivity in the top 30 cm of soil during all growth stages. Both SM and BC significantly enhanced the total nutrient availability for barley and cotton, especially improving soil organic carbon and available potassium, with BC showing notable improvements. At barley’s heading stage, SI maximized dry matter accumulation, while SM boosted accumulation in leaves, stems, and spikes during the filling and maturation stages. Straw returning increased barley yield, particularly with SM and BC, and improved water use efficiency by 11.60% and 5.74%, respectively. For cotton, straw returning significantly boosted yield and water use efficiency, especially with SI and SM treatments, enhancing the total bolls and yield. In conclusion, straw returning effectively improves saline soils, enhances fertility, boosts crop yields, and supports sustainable agriculture. These results provide a robust scientific foundation for adopting efficient soil improvement strategies on saline lands, with significant theoretical and practical implications for increasing agricultural productivity and crop resilience to salt stress.

Identification of New Isolates of Phytophthora sojae and Selection of Resistant Soybean Genotypes.

Phytophthora root and stem rot (PRR), caused by Phytophthora sojae, can occur at any growth stage under poorly drained and humid conditions. The expansion of soybean cultivation in South Korean paddy fields has increased the frequency of PRR outbreaks. This study aimed to identify four P. sojae isolates newly collected from domestic fields and evaluate race-specific resistance using the hypocotyl inoculation technique. The four isolates exhibited various pathotypes, with GJ3053 exhibiting the highest virulence complexity. Two isolates, GJ3053 and AD3617, were screened from 205 soybeans, and 182 and 190 genotypes (88.8 and 92.7%, respectively) were susceptible to each isolate. Among these accessions, five genotypes resistant to both isolates were selected. These promising genotypes are candidates for the development of resistant soybean cultivars that can effectively control PRR through gene stacking.

The accumulation of cadmium and lead in wheat grains is primarily determined by the soil-reducible cadmium level during wheat tillering

The significant increase in cadmium (Cd) and lead (Pb) pollution in agricultural soil has greatly heightened environmental contamination issues and the risk of human diseases. However, the mechanisms underlying the transformation of Cd and Pb in soil as well as the influencing factors during their accumulation in crop grains remain unclear. Based on the analysis of the distribution trend of Cd and Pb in soil during the growth and development stages of wheat (tillering, filling, and maturity) in alkaline heavy metal-polluted farmland in northern China, this study investigated the response mechanism of soil heavy metal form transformation to soil physicochemical properties, and elucidated the main determining periods and influencing factors for Cd and Pb enrichment in wheat grains. The results showed that an increase in CEC and SOM levels, along with a decrease in pH level, contributed to enhancing the bioavailability of Cd in the soil. This effect was particularly evident during the tillering stage and grain filling stage of wheat. Nevertheless, the effects of soil physicochemical properties on bioavailable Pb was opposite to that on bioavailable Cd. The enrichment of Cd and Pb in grain was significantly influenced by soil pH (r = −0.786, p < 0.01), SOM (r = 0.807, p < 0.01), K (r = −0.730, p < 0.01), AK (r = 0.474, p = 0.019), and AP (r = −0.487, p = 0.016). The reducible form of Cd in soil during the wheat tillering stage was identified as the primary factor contributing to the accumulation of Cd and Pb in wheat grains, with a significant contribution rate of 84.5%. This study provides a greater scientific evidence for the management and risk control of heavy metal pollution in alkaline farmland.

Robust soybean leaf agroinfiltration.

A robust agroinfiltration-mediated transient gene expression method for soybean leaves was developed. Plant genotype, developmental stage and leaf age, surfactant, and Agrobacterium culture conditions are important for successful agroinfiltration. Agroinfiltration of Nicotiana benthamiana has emerged as a workhorse transient assay for plant biotechnology and synthetic biology to test the performance of gene constructs in dicot leaves. While effective, it is nonetheless often desirable to assay transgene constructs directly in crop species. To that end, we innovated a substantially robust agroinfiltration method for Glycine max (soybean), the most widely grown dicot crop plant in the world. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium strain and culture medium. Our optimized protocol involved a multi-step Agrobacterium culturing process with appropriate expression vectors, Silwet L-77 as the surfactant, selection of fully expanded leaves in the VC or V1 stage of growth, and 5min of vacuum at -85kPa followed by a dark incubation period before plants were returned to normal growth conditions. Using this method, young soybean leaves of two lines-V17-0799DT, and TN16-5004-were high expressors for GUS, two co-expressed fluorescent protein genes, and the RUBY reporter product, betalain. This work not only represents a new research tool for soybean biotechnology, but also indicates critical parameters for guiding agroinfiltration optimization for other crop species.We speculate that leaf developmental stage might be the most critical factor for successful agroinfiltration.

Screening of lentil genotypes during germination and early growth stages under PEG-induced drought stress

Screening of lentil genotypes during germination and early growth stages under PEG-induced drought stress

Analytical strategies to identify multicomponent quality markers for commercial Hua-ju-hong using multidimensional chemical analysis.

Hua-ju-hong (HJH) is a Chinese medicinal material obtained from Citrus grandis 'Tomentosa' (CGT) and Citrus grandis (L.) Osbeck (CG) with various commercial specifications. It is known for relieving cough and dispelling phlegm. To reveal the quality marker for distinguishing the various HJH, 215 batches of commercial HJH were studied systematically using multidimensional chemical analysis. Ten major components were identified by high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and quantified via high-performance liquid chromatography coupled with diode array detection. In this study, a rapid, efficient, and low-cost chromatographic method was established. Total coumarin-hemiterpene and total coumarin-monoterpene were first classified and analyzed in HJH. The result indicated that the main component, naringin, was not the quality marker for differentiating CGT from CG. For reflecting the unique medicinal and food value of HJH, coumarins should be the more potential quality markers. Flavonoids were the possible quality markers for distinguishing two growth stages of fruit-exocarp and young fruit. For the first time, two chemotypes of HJH were identified in CG. This study provides a convenient yet reliant chromatographic method and novel yet systematic strategies for overall quality control of commercial HJH.

Evaluation of nutritional composition, biochemical, and quality attributes of different varieties of tomato (Solanum lycopersicum L.)

Due to the wide variety and great quality differences, it is very confusing for consumers and growers to choose the best variety. For this purpose, we conducted a greenhouse test to focus on the nutrients and bioactive substances of diverse varieties of tomato. Using high-performance liquid chromatography (HPLC), we analyzed 28 quality traits across the nine tomato varieties. The results revealed significant variations in quality attributes of nine varieties, such as naringenin and cinnamic acid content, ranging from 0 to 2.02 and 0.97 μg/g, respectively. Similarly, sucrose, malic acid, lycopene, β-carotene, and gentisic acid exhibited high coefficients of variation of 31.60 %, 74.82 %, 30.94 %, 39.59 %, and 86.32 %, respectively. For the comprehensive evaluation of quality and difference among tomato varieties, we used Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) and Principal Component Analysis (PCA) combined with membership function methods. Notably, strawberry tomato (S2) and Yuanwei No.1 (S1) exhibited the highest quality, and Pink Belle No.2 (S7) showed lowest. The above metabolites show different accumulations potential among different varieties. In addition, we explored the nutritional variations in tomatoes different parts at various growth stages of two varieties. Overall, study result investigated metabolic differences of nutrients at different growth stages, especially ripening stage affecting fruit quality. Thus, these useful findings will provide useful information for consumers and researchers.

Calliobothrium Narsisae n. sp. (Tetraphyllidea: Calliobothriidae) from the Triakid Shark Mustelus mosis Hemprich and Ehrenberg from the Persian Gulf, with a Note on Its Specific Proglottisation Process and a Key to the Species of the Genus.

The members of the tetraphyllidean genus Calliobothrium exhibit a high degree of host specificity in the shark genus Mustelus. In the Indian Ocean, where M. mosis occurs dominantly, there is no information on Calliobothrium. The purpose of this study was the evaluation of the Calliobothrium species in M. mosis in the Persian Gulf, northwestern Indian Ocean. Nine specimens of Mustelus mosis were caught from the Persian Gulf. The tapeworms isolated were stained, measured, and the line drawings were made. Calliobothrium narsisae n. sp. is described and differs from C. creeveyae in the intact, rather than the three-lobed, posterior bothridial margins, from C. tylotocephalum in possessing three, rather than one, suckers per bothridial muscular pad, from C. nodosum in possessing the elongated, rather than robust and thorn-like, hooks, from C. shirozame in a maximum number of four, rather than three, dorsal and four, rather than three, ventral laciniations along the strobila, and from its other congeners in possessing three, rather than two, dorsal and three, rather than two, ventral laciniations in the anteriormost immature proglottids. A key to the species of the genus is presented and the proglottisation process, by the germinative zone placed in the terminal part of the worm at least in some stages of growth to maturity, is discussed. The description of C. narsisae n. sp., which brings the total number of valid species of Calliobothrium to 10, also indicates that the germinative zone is not always at the anterior to strobila and in this new species it is placed in the terminal part of the worm at least in some stages of growth to maturity.

Effect of Herbal Kunapajala and Different Organic Manures on Plant Growth, Quality and Yield of Withania Somnifera L. Dunal

Effect of Herbal Kunapajala and Different Organic Manures on Plant Growth, Quality and Yield of Withania Somnifera L. Dunal

Variability of Root and Shoot Traits under PEG-Induced Drought Stress at an Early Vegetative Growth Stage of Soybean

Variability of Root and Shoot Traits under PEG-Induced Drought Stress at an Early Vegetative Growth Stage of Soybean

Full-Length Transcriptome Analysis of Skeletal Muscle of Jiangquan Black Pig at Different Developmental Stages.

Skeletal muscle grows in response to a combination of genetic and environmental factors, and its growth and development influence the quality of pork. Elucidating the molecular mechanisms regulating the growth and development of skeletal muscle is of great significance to both animal husbandry and farm management. The Jiangquan black pig is an excellent pig breed based on the original Yimeng black pig, importing the genes of the Duroc pig for meat traits, and cultivated through years of scientific selection and breeding. In this study, full-length transcriptome sequencing was performed on three growth stages of Jiangquan black pigs, aiming to study the developmental changes in Jiangquan black pigs at different developmental stages at the molecular level and to screen the key genes affecting the growth of skeletal muscle in Jiangquan black pigs. We performed an enrichment analysis of genes showing differential expression and constructed a protein-protein interaction network with the aim of identifying core genes involved in the development of Jiangquan black pigs. Notably, genes such as TNNI2, TMOD4, PLDIM3, MYOZ1, and MYH1 may be potential regulators of muscle development in Jiangquan black pigs. Our results contribute to the understanding of the molecular mechanisms of skeletal muscle development in this pig breed, which will facilitate molecular breeding efforts and the development of pig breeds to meet the needs of the livestock industry.

Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta)

Taro is a widely utilized starch resource plant. It is essential to quantify the expression levels of functional genes associated with taro growth using real-time quantitative polymerase chain reaction (RT-qPCR). However, to obtain reliable RT-qPCR results, appropriate reference genes (RGs) are required for data normalization. In this study, we screened seven novel candidate RGs using transcriptome datasets from taro, encompassing data from growth corms and various tissues. The expression stability of these seven new RGs, along with the commonly used RGs Actin, EF1-α, and β-tubulin, was assessed using Delta Ct, BestKeeper, geNorm, and NormFinder algorithms. Furthermore, we conducted a comprehensive analysis using the RefFinder program and validated the results using the target gene, CeAGPL1. The findings revealed that ACY-1 and PIA2 were the optimal multiple RGs for normalization during corm growth, while COX10 and Armc8 were suitable for samples including various types of tissues. Furthermore, we found three RGs, Armc8, COX10 and CCX4L, were the optimal RGs for drought stress. This study assessed the suitability of RGs in taro for the first time. The identified RGs provide valuable resources for studying corm growth, diverse tissues, and drought stress. This study contributes to the advancement of our understanding of the underlying mechanisms that govern the growth of taro.

Flexible Resource Allocation-Efficient Water Use Strategies Facilitate Invasion of Invasive Vine Sicyos angulatus L.

The invasive vine Sicyos angulatus L. destroys the natural ecosystem of invaded areas. Understanding the differences in growth and development between S. angulatus and other plants is necessary to explore the invasion mechanisms of S. angulatus and implement appropriate prevention and control measures. Thus, this study compared the growth, photosynthesis, and root characteristics of invasive liana S. angulatus and other three vine plants, Ipomoea nil (L.) Roth, Ipomoea purpurea (L.), and Thladiantha dubia Bunge, at different growth stages: seedling, flowering, and fruiting. The results showed that the total biomass of S. angulatus in the fruiting stage was 3-6 times that of the other three plants, and the root biomass ratio and root-shoot ratio decreased throughout the growth stage. Throughout the growth stage, the total leaf area of S. angulatus was significantly higher than that of the other three plant types, and the specific leaf area of S. angulatus at the seedling and flowering stages was 2.5-3 and 1.4-3 times that of the other three plants, respectively. The photosynthetic rate, stomatal conductance, and transpiration rate of S. angulatus at the fruiting stage were significantly higher than those of the other three plants, and its water use efficiency was higher than that of the other three plants at the three growth stages, indicating its strong photosynthetic capacity. The root activity and root pressure of S. angulatus were also significantly higher than those of the other three plants at the seedling and flowering stages. These results show that S. angulatus flexibly allocates resources to its aboveground parts during the growth stage to ensure that the plant obtains the space necessary for its growth and development and that with the help of higher root pressure and root activity, S. angulatus can maintain higher photosynthesis and water use efficiency with fewer resources. Therefore, the prevention and control of S. angulatus requires a combination of aboveground and underground measures. Spraying conventional weedicide/herbicide and manually removing aboveground plants may lead to its resurgence.

Unraveling the reasons for failure to control Amaranthus albus: insights into herbicide application at different growth stages, temperature effect, and herbicide resistance on a regional scale.

This study investigates factors contributing Amaranthus albus control failure in processing tomato fields in northern Israel. The study region is characterized by a significant climate gradient from east to west, providing the opportunity to investigate the effect of critical elements of the agricultural environment, e.g., temperature. Eight populations were collected from commercial fields in this region. Post-emergence herbicide efficacy of metribuzin, a photosystem II inhibitor, and rimsulfuron, an acetolactate synthase (ALS) inhibitor, was assessed through dose-response analyses at various growth stages. Temperature effects on control efficacy and resistance mechanisms were also explored. Standard metribuzin dose (X) was ineffective on A. albus plants with more than six true-leaves, whereas 2X dose proved effective. Rimsulfuron at 16X dose was ineffective on plants with more than four true-leaves. We report here the first case of target site resistance to ALS inhibitors in A. albus, due to point mutation in the ALS gene (Pro197 to Leu). Furthermore, our findings suggest potential involvement of CYT P450 enzymes in enhanced metabolizing of rimsulfuron. An overall decrease in dry weight was observed in response to both herbicides at 16/22 °C (P < 0.0001). Rimsulfuron was effective against only one population when applied at 28/34 °C. A possible fitness cost associated with target site-resistant biotypes was observed under low temperature conditions, leading to effective control. This regional-scale study highlights the challenges faced by growers, emphasizes the need for adapting management practices to the local climatic conditions and lays the groundwork for implementing location-specific weed management strategies in commercial fields. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Response Surface Methodology for Development of Nutrient Solution Formula for Hydroponic Lettuce Based on the Micro-Elements Fertilizer Requirements at Different Growth Stages

In order to precisely obtain the impact of nutritional elements on lettuce yield and quality, in the present study, a nutrient solution formula suitable for lettuce hydroponic production was development using response surface methodology based on the determination for micro-elements in three growth stages and taking the interaction between elements into account. Then, the formula was optimized and validated, aiming for the goal of improving lettuce yield and quality. The results showed that 200 healthy lettuce leaves contained similar amounts of macro-elements, and there was no significant difference in the unit content of micro-elements among the seedling, rosette, and harvest stages. Quadratic regression models between shoot fresh weight, SPAD value, soluble sugar content, Vc content, and nutrient content were established (R2 = 0.91, 0.95, 0.98, and 0.81, respectively). The optimal concentrations of P, K, Ca, and Mg obtained by multi-objective optimization of the quadratic regression models for fresh weight, SPAD value, soluble sugar content, and Vc content were 2.71 mmol·L−1, 6.42 mmol·L−1, 5.58 mmol·L−1, and 7.11 mmol·L−1, respectively. The nutrient solution formula (T1) was found to be the optimal nutrient solution formula for improving lettuce growth and quality. Overall, we developed a specific and targeted nutrient solution formulation for lettuce; this formulation not only meets lettuce’s demand for nutrients, but also improves lettuce yield and quality, providing more choices for lettuce production in a region with high salts and high pH in the irrigation water.

Calamitean Cones and Their In Situ Spores from the Pennsylvanian Limnic Basins of the Czech Republic.

This paper describes the in situ spores of the Calamospora type, macerated from sixty-one specimens of calamitean cones belonging to sixteen species of genera, such as the Palaeostachya, Macrostachya, Calamostachys, and Huttonia from the Pennsylvanian Czech Republic period, specifically from the Moscovian/Kasimovian ages (i.e., Duckmantian-Stephanian). The in situ spores were compared to twenty dispersed species of Calamospora. The majority of spores were microspores; however, some cones yielded both micro- and megaspores. Morphological variations of the in situ spores, including the diameter, labrum, contact area, ontogenetic stages, and secondary folds of the exine, are described, including their importance for the classification of calamospores. The relationships of Elaterites, Pteroretis, Vestispora, and some monopseudosaccate spores are discussed. All Paleozoic Calamospora-producing parent plants are summarized.

Inversion of Glycyrrhiza Chlorophyll Content Based on Hyperspectral Imagery

Glycyrrhiza is an important medicinal crop that has been extensively utilized in the food and medical sectors, yet studies on hyperspectral remote sensing monitoring of glycyrrhiza are currently scarce. This study analyzes glycyrrhiza hyperspectral images, extracts characteristic bands and vegetation indices, and constructs inversion models using different input features. The study obtained ground and unmanned aerial vehicle (UAV) hyperspectral images and chlorophyll content (called Soil and Plant Analyzer Development (SPAD) values) from sampling sites at three growth stages of glycyrrhiza (regreening, flowering, and maturity). Hyperspectral data were smoothed using the Savitzky–Golay filter, and the feature vegetation index was selected using the Pearson Correlation Coefficient (PCC) and Recursive Feature Elimination (RFE). Feature extraction was performed using Competitive Adaptive Reweighted Sampling (CARS), Genetic Algorithm (GA), and Successive Projections Algorithm (SPA). The SPAD values were then inverted using Partial Least Squares Regression (PLSR), Support Vector Regression (SVR), Random Forest (RF), and Extreme Gradient Boosting (XGBoost), and the results were analyzed visually. The results indicate that in the ground glycyrrhiza inversion model, the GA-XGBoost model combination performed best during the regreening period, with R2, RMSE, and MAE values of 0.95, 0.967, and 0.825, respectively, showing improved model accuracy compared to full-spectrum methods. In the UAV glycyrrhiza inversion model, the CARS-PLSR combination algorithm yielded the best results during the maturity stage, with R2, RMSE, and MAE values of 0.83, 1.279, and 1.215, respectively. This study proposes a method combining feature selection techniques and machine learning algorithms that can provide a reference for rapid, nondestructive inversion of glycyrrhiza SPAD at different growth stages using hyperspectral sensors. This is significant for monitoring the growth of glycyrrhiza, managing fertilization, and advancing precision agriculture.

Recent progress in the role of seed endophytic bacteria as plant growth-promoting microorganisms and biocontrol agents.

The importance of microorganisms residing within the host plant for their growth and health is increasingly acknowledged, yet the significance of microbes associated with seeds, particularly seed endophytic bacteria, remains underestimated. Seeds harbor a wide range of bacteria that can boost the growth and resilience of their host plants against environmental challenges. These endophytic associations also offer advantages for germination and seedling establishment, as seed endophytic bacteria are present during the initial stages of plant growth and development. Furthermore, plants can selectively choose bacteria possessing beneficial traits, which are subsequently transmitted through seeds to confer benefits to future generations. Interestingly, even with the ongoing discovery of endophytes in seeds through high-throughput sequencing methods, certain endophytes remain challenging to isolate and culture from seeds, despite their high abundance. These challenges pose difficulties in studying seed endophytes, making many of their effects on plants unclear. In this article, a framework for understanding the assembly and function of seed endophytes, including their sources and colonization processes was outlined in detail and available research on bacterial endophytes discovered within the seeds of various plant species has also been explored. Thus, this current review aims to provide valuable insights into the mechanism of underlying seed endophytic bacteria-host plant interactions and offers significant recommendations for utilizing the seed endophytic bacteria in sustainable agriculture as plant growth promoters and enhancers of environmental stress tolerance.