Growth Of Streptococcus Mutans Research Articles

Effect of Chitosan and Acrylic Acid Addition to Acrylic Resin on Porosity and Streptococcus mutans Growth in Denture Base.

This work aimed to determine the effect of adding chitosan and acrylic acid to acrylic resin denture base on the porosity of the material and the growth of Streptococcus mutans. This study is an experimental laboratory research. Samples were divided into the following three groups (n = 10): group 1 was the control group, group 2 was the acrylic resin mixture with 1% chitosan and acrylate acid, and group 3 was the acrylic resin mixture with 2% chitosan and acrylate acid. S. mutans growth was tested using the dilution method, and porosity was examined using an optical microscope. Data were calculated by one-way analysis of variance (p < 0.05) and correlation analysis. The acrylic resin added with 2% chitosan and acrylic acid showed pores with an almost spherical shape and the smallest size. Significant difference (p < 0.05) was observed among all the groups. A positive and extremely strong correlation was found between porosity and S. mutans growth. Chitosan and acrylic acid at 1 and 2% concentrations can be added to acrylic resin to minimize the porosity of the denture base and reduce the growth of S. mutans. A less porous denture is associated with a low S. mutans growth rate.

Effects of a Novel, Intelligent, pH-Responsive Resin Adhesive on Cariogenic Biofilms In Vitro.

Background: Secondary caries often result in a high failure rate of resin composite restoration. Herein, we studied the dodecylmethylaminoethyl methacrylate–modified resin adhesive (DMAEM@RA) to investigate its pH-responsive antimicrobial effect on Streptococcus mutans and Candida albicans dual-species biofilms and on secondary caries. Methods: Firstly, the pH-responsive antimicrobial experiments including colony-forming units, scanning electron microscopy and exopoly-saccharide staining were measured. Secondly, lactic acid measurement and transverse microradiography analysis were performed to determine the preventive effect of DMAEM@RA on secondary caries. Lastly, quantitative real-time PCR was applied to investigate the antimicrobial effect of DMAEM@RA on cariogenic virulence genes. Results: DMAEM@RA significantly inhibited the growth, EPS, and acid production of Streptococcus mutans and Candida albicans dual-species biofilms under acidic environments (p < 0.05). Moreover, at pH 5 and 5.5, DMAEM@RA remarkably decreased the mineral loss and lesion depth of tooth hard tissue (p < 0.05) and down-regulated the expression of cariogenic genes, virulence-associated genes, and pH-regulated genes of dual-species biofilms (p < 0.05). Conclusions: DMAEM@RA played an antibiofilm role on Streptococcus mutans and Candida albicans dual-species biofilms, prevented the demineralization process, and attenuated cariogenic virulence in a pH-dependent manner.

Effect of Nutmeg (Myristica Fragrans) Methanolic Extract to the Growth of Dental Plaque Bacteria

Background: One of the causes of dental caries is Streptococcus mutant bacteria which has the ability to change the sugar content of food waste into lactic acid as the initial process of caries. One of the efforts to overcome dental caries is to use natural ingredients such as nutmeg. Every part of nutmeg has active substances that are efficacious as antimicrobial, antibacterial, antioxidant, antifungal and anti-inflammatory. Pulpam, seeds and fuli of nutmeg showed as potential extracts in inhibiting the growth of Streptococcus mutans. Objective: This study aimed to analyze methanol extracts from pericarpium, pulpam, fuli and nutmeg seeds against the growth of Streptococcus mutans bacteria that causes dental plaque. Methods: The research was conducted by diffusion method using BHI agar media and incubated anaerobically at 37°C for 24 hours. Results: The results of the calculation of the average diameter of the inhibition zones from the extract of fuli, seeds, pulpam and pericarpium of the nutmeg plant were 19.00 mm, 25.33 mm, 15.66 mm, 22.66 mm, and 21.83 mm, respectively. Data analysis using ANOVA (one way) showed that there were significant differences in all groups at p&lt;0.05. The results of the LSD test showed that there were significant differences in all treatment groups. Conclusion: Methanol extract from fruit and fruit parts of nutmeg showed the ability to inhibit the growth of bacteria that cause dental plaque.

The effect of using Laban leaf (Vitex pinnata) methanol extract toothpaste on saliva pH in Students of SD Negeri I Pagar AIR

Background: Maintaining the pH balance of saliva is one way to prevent dental caries. The pH balance of saliva is influenced by the rate of salivary secretion. The rate of salivary secretion can be stimulated chemically and mechanically. Mechanical stimulation by brushing teeth and chemically by gargling. Indonesia is currently developing natural ingredients as medicinal ingredients that have been scientifically tested for their benefits and safety. Laban leaf (Vitex pinnata) is one of the many medicinal plants found in Indonesia. Laban leaves contain alkaloids, flavonoids, saponins, and tannins that can inhibit the growth of Streptococcus mutans so that it can remove dental plaque naturally. Some researchers state that laban leaf extract can affect the pH of saliva. This study aims to see the effect of the use of laban leaf methanol extract toothpaste on salivary pH.Methods: Experimental research method with pre and post-tests design with proposing sampling technique where 26 people were taken as samples. The sample was divided into 2 groups which received different treatment. Group 1 was instructed to brush their teeth with laban leaf extract toothpaste and group 2 with toothpaste circulating in the market tied their teeth for 3 minutes.Results: Results based on the comparison of salivary pH before and after brushing teeth, it was found that brushing teeth with methanol extract toothpaste of laban leaves was more effective in increasing salivary pH. Based on the use of toothpaste with laban leaf methanol extract, the significant value is 0.000&lt;(0.05), so it can be concluded that reject H0. This means that there is an average difference before and after using laban leaf methanol extract toothpaste. In conclusion, the use of laban leaf methanol extract toothpaste has more effect on increasing the pH of saliva.Conclusions: brushing teeth with laban leaf methanol extract toothpaste is more effective in increasing the pH of saliva. We recommend recommending further research to determine the potential of laban leaves to be used as raw material for toothpaste.

Development and evaluation of a chewing gum containing antimicrobial peptide GH12 for caries prevention.

The purpose of this study was to develop a chewing gum containing a novel antimicrobial peptide GH12 and evaluate its biocompatibility, antimicrobial activity, and caries-preventive effects in vivo and in vitro. GH12 chewing gum was developed using a conventional method and its extracts were prepared in artificial saliva. GH12 concentration in the extracts was determined by high-performance liquid chromatography; extracts were used for growth curve assay, time-kill assay, crystal violet staining assay, scanning electron microscopy, and Cell Counting Kit-8 assay. A rat caries model was established, and molars were treated topically with extracts for 5weeks. Weight gain monitoring, hematoxylin-eosin staining, micro-computed tomography, and Keyes scoring were conducted. Significant inhibition of Streptococcus mutans growth and biofilm formation was observed. Extracts displayed low cytotoxicity against human gingival epithelial cells. No significant differences in weight gain or signs of harm to the mucosal tissues in any of the rats were observed. Keyes scores of caries lesions in the GH12 chewing gum group were lower than those of the negative control group.It was concluded that GH12 chewing gum showed good biocompatibility, antimicrobial activity, and caries-preventive effects, exhibiting great potential to prevent dental caries as an adjuvant to regular oral hygiene.

Local anaesthetic inhibition of lidocaine on the growth of streptococcus mutans in vitro

Objective: This study aims to determine the effectiveness of lidocaine of local anaesthetic agent in inhibiting the growth of streptococcus mutans bacteria.&#x0D; Material and Methods: This type of research is a laboratory experimental with a post test only control group design. Microorganism used was streptococcus mutans and cultured at the Microbiology Laboratory of the Pharmacy, Universitas Sumatera Utara. The local anaesthetic tested was a solution of lidocaine diluted with NaCl 0.9% to 0.5%, 1%, and 2%. The antibacterial activity test was carried out using diffusion method by dripping 25 µl of lidocaine (0.5%, 1%, 2%), positive control (amoxicillin), and negative control (NaCl 0.9%) on disc paper, then placed on nutrient agar, and incubated. After 24 hours, the inhibition zone formed around the disc paper was observed.&#x0D; Results: Lidocaine showed antibacterial activity with the inhibition zone of 0.5%, 1%, 2% concentration was 6.98 mm, 7.66 mm, 8.36 mm. Then the data was analyzed using the One-way ANOVA test. From these results, there was a significant difference in the mean of the inhibitin zone (p &lt; 0.05) between 0.5%, 1%, and 2% lidocaine, positive control, and negative control.&#x0D; Conclusion: Lidocaine has antibacterial activity against Streptococcus mutans bacteria with an effective concentration of 2%.

Polyphenol-Enriched Extract of Lacquer Sap Used as a Dentine Primer with Benefits of Improving Collagen Cross-Linking and Antibacterial Functions.

Commercial dentin adhesive systems are applied to restorations due to their resistant bonding properties, but they suffer from the lack of bioactivity and are prone to hydrolysis. Therefore, to overcome these limitations, an eco-friendly natural monomer, urushiol, was adopted to be a primer in dentin bonding due to its interaction with collagen and antibacterial activity, preventing further hydrolysis development. First, urushiol was determined to be capable of improving the biological stability of dentin collagen through cross-linking. Using high-fidelity analytical chemistry techniques, such as Fourier transform infrared spectroscopy, we quantified the effects of urushiol on collagen molecules. It could also effectively decrease weight loss after collagenase ingestion by improving the stability of dentin. Moreover, urushiol inhibited Streptococcus mutans growth as well as its biofilm formation. Finally, we demonstrated that the urushiol primer could improve the bonding strength, particularly after aging. The cross-linking and antibacterial functions of urushiol have provided promising developmental prospects for biomaterials in dentin adhesion.

Antibacterial Activity of Edible Film with the Addition of Betel Leaf Extract (Piper betle) againts Streptococcus mutans

Edible films are generally used as packaging materials to protect food and can be eaten with the packaged product. In addition, the edible film can be affixed to the tongue, so that it immediately melts in the mouth. Products like this can be added to betel leaf extract which can function as an oral antiseptic, that can inhibit the growth of Streptococcus mutans. The purpose of this study was to obtain the results of the inhibition of edible film added with water extract of betel leaf with concentrations of 30%, 50%, and 70%. The research was conducted through experiments in the laboratory by measuring the diameter of the inhibition zone that occurred. The diameter of the average inhibition zone against Streptococcus mutans produced by edible film added 30%, 50%, and 70% aqueous extract of betel leaf, respectively, was 14.36 mm, 18.17 mm, and 24.74 mm. An edible film added water extract of betel leaf can inhibit Streptococcus mutans.

ANTIBACTERIAL POTENTIAL OF LIME (Citrus aurantifolia) ON THE GROWTH OF Streptococcus mutans

Streptococci have a variety of solid virulence factors that allow various infections to occur. Streptococcus mutans is an obligate biofilm organism and is considered the primary etiology of dental caries. Lime (Citrus aurantifolia) is widely consumed because of its natural safety factors and is a vital ingredient in most herbal ingredients with antibacterial properties. Prevention in dentistry needs to be improved, one of which is through the potential of herbal plants to reduce world health problems, especially in Indonesia. Objective To determine the possibility of lime antibacterial compounds inhibiting the growth of Streptococcus mutans. Streptococcal groups, especially S. mutans, are aciduric and acidogenic organisms and can create environmental conditions that lethal to other bacteria. Streptococcus mutans produces several extracellular sucrose metabolite enzymes that contribute to the organism's ability to cause dental caries. Streptococcal virulence factors are usually due to adesin attachment to cell surface proteins, glucosyltransferase (GTF) production, acid production, and acid tolerance. Oranges are one of the largest plant species distributed throughout the world. Plants of the citrus genus are mainly valued for their edible fruit and traditional medicinal value and have an important role in health. Lime is a herbal plant that has the potential as an antibacterial against streptococci because it contains beneficial active compounds such as saponins, flavonoids, citric acid, amino acids, essential oils, resins, glycosides, vitamin B1, vitamin C, citric acid, fats, iron, calcium, sulfur, phosphorus, and is expected to be a choice of chemical drugs that have many side effects. Conclusion Lime is a herbal plant that contains antibacterial compounds that show various biological activities, are proteolytic and lipolytic, and contain active antibacterial compounds that are expected to reduce the incidence of dental and oral diseases.

The Effect Of Lemongrass Stem Infusion (Cymbopogon citratus) On Growth Of Streptococcus mutans

Objectives: To determine the effect of lemongrass infusion on the growth of Streptococcus mutans at various concentrations. Methods: This study used post test only control group design with total sample is 9 samples, concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, positive control, and negative control with 3 replications. The research uses dilution method by looking at the turbidity which can be marked as MIC, for MBC used pour plate method was determined by looking at the presence or absence of bacterial growth on the plate. Data analysis was performed using the spss application using Kruskal-Wallis test with significance level if the result of p&lt;0,05. Results of Study: The results of the sample group were 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56% in the MIC test showed turbidity in all samples, then the MBC test showed growth bacteria in all samples. The assessment of the probability test p value of 1,000 (&gt;0,05) indicates that there is no significant difference between research groups. Conclusion: There was no effect of lemongrass stem infusion with various concentrations on the growth of Streptococcus mutans.

Antibacterial activity of Solanum macrocarpon against clinically derived isolates of Streptococcus mutans from patients attending university of Maiduguri teaching hospital: Implantation of correlation analysis prior to mathematical and computational appro

Plants have been employed as herbal medicines in many parts of the world, and this tend to gain attention as complementary or alternative to modern medicines. This study is aimed to assess the antibacterial activity ofSolanum macrocarpon against Streptococcus mutans. Standard microbiological and biochemical techniques were used to isolates and identify Streptococcus mutans. The Distilled water, Ethanol, Methanol extraction were performed on different part of Solanum macrocarpon and tested for antibacterial activity using the Agar well diffusion method. All the extracts inhibit the growth of Streptococcus mutans except distilled water extract with no or little effect. The results also showed an increase in antibacterial activity with increase in concentration21 mm at 100% concentration to 4 mm of the crude extracts. This study therefore revealed that Solanum macrocarponextract demonstrated greet inhibitory effect on the test organism. Thus, the result provides a basis for the traditional use of Solanum macrocarpon in cleaning teeth and toothache treatment.

TESTING THE RESPONSIBILITY OF EGGPLANT THORN (SOLANUM CAROLINENSE) ETHANOL EXTRACT ON THE GROWTH OF BACTERIA (STREPTOCOCCUS MUTANS) IN VITRO

Streptococcus mutans is the main pathogenic bacteria causing caries and tooth enamel damage. Eggplant spines (Solanum carolinense) contains active substances that can inhibit the growth of Streptococcus mutans bacteria on teeth. The purpose of this study was to determine the inhibitory effect of the antibacterial activity of ethanol extract of thorn eggplant on the growth of Streptococcus mutans bacteria and to determine the optimal concentration of thorn eggplant extract on the growth of Streptococcus mutans bacteria in vitro. The type of method used in this study is an experimental study, with the dependent variable being the zone of inhibition of the growth of Streptococcus mutans, the independent variable being differences in the concentration of ethanol extract of thorn eggplant (Solanum carolinense) at concentrations of 20%, 40%, 60%, 80% and 96 ethanol. % as negative control and chloramphenicol as positive control, control variables for the presence of other microbial contamination, media thickness, turbidity of bacterial suspension, incubation temperature, disc distance, incubation time, sterilization of equipment, media and room. The results of this study were the effect of the ethanol extract of thorn eggplant on Streptococcus mutans and the optimal concentration of the ethanolic extract of thorn eggplant was 80% with an inhibition zone diameter of 11 mm (strong) which was indicated by the appearance of the diameter of the bacterial inhibition zone at each treatment

Calcium Phosphate Cement Promotes Odontoblastic Differentiation of Dental Pulp Cells In Vitro and In Vivo

In the case of pulp injury, odontoblastic differentiation of dental pulp cells (DPCs) at the site of the exposed pulp is necessary for a successful direct pulp capping treatment. Calcium phosphate cement (CPC), a kind of hydroxyapatite-like bone cement, exhibits therapeutic potential in osteogenesis by regulating cell cycle progression and promoting osteoblastic differentiation. Based on the similar biological process of osteo/odontoblastic differentiation, the present study evaluated the effects of CPC on odontoblastic differentiation of DPCs in vitro and in vivo, respectively. The morphology of CPC was observed by scanning electron microscopy. Colony-forming units were used to assess the antibacterial activity. The effects of CPC on cell proliferation and odontoblastic differentiation of human dental pulp cells (hDPCs) were also measured. Histological staining was performed to observe the reparative dentin formation in rat molars. In vitro, results of antibacterial studies showed that CPC significantly inhibited the growth of Streptococcus mutans. The appropriate concentration of CPC extract showed low cytotoxicity on hDPCs. Furthermore, CPC extract also promoted odontoblastic differentiation and mineralization compared with the control group, as shown by a dynamic increase in the expression of odontogenic marker genes and the increased number of mineralized nodules at 21 days. The pulpotomy models with CPC facilitated the formation of dentin bridge with the highly expressed dentin matrix protein 1 (DMP1) in odontoblast-like cells. In conclusion, the favorable biocompatibility, antibacterial property and bio-inductivity of CPC suggest that CPC can be used as a promising direct pulp capping material.

Development of an eco-sustainable formulation against Streptococcus mutans and Candida albicans

The acidic niche of dental plaque can lead to caries expression over time. In this study, we attempted to design an eco-sustainable oral care formulation, and to determine its effect on caries-related pathogens in the presence and absence of lactic acid. We investigated the inhibitory effect of slightly acidic electrolyzed water (SAEW) combined with sodium benzoate (NaB) on the growth of caries-related microorganisms (both bacteria and fungi). SAEW’s antimicrobial activity was significantly increased in the presence of lactic acid (4.0 log CFU/mL for Streptococcus mutans, and 5.31 log CFU/mL for Candida albicans). In the presence of lactic acid, 0.1% of NaB completely inhibited the growth of Streptococcus mutans and Candida albicans, while there was no significant inhibitory effect in the absence of lactic acid. The results showed that SAEW combined with NaB had high antimicrobial activity in the acidic environment but low efficacy in the normal niche. Thus, this combination may have fewer negative effects on oral microbiota diversity. This is the first study to investigate the possibility of SAEW as alternatives to alcohol-based oral hygiene products.

The antimicrobial and antibiofilm effects of three herbal extracts on Streptococcus mutans compared with Chlorhexidine 0.2% (in vitro study).

There is a special focus on using natural materials and herbal plants to prevent dental caries. Previous studies showed that some herbal plants have antimicrobial effects on oral pathogens. Thus we investigated the antimicrobial effects of three herbal extracts (Carum copticum, Phlomis bruguieri, and Marrubium parviflorum) on the growth of Streptococcus mutans, as the most important bacteria causing dental caries. First, plant methanolic extracts were prepared. Then, to evaluate the antimicrobial activity of the three herbal extracts, the agar well diffusion method and MIC were performed. The biofilm formation was carried out using a broth dilution method with 2% glucose-supplemented BHIS in sterile 96-well microplates. Serial dilutions (50, 25, 12.5, 6.25, 3.12 mg/ml) of extracts were prepared. Next, a 0.5 McFarland Suspension of S. mutans was added to wells. The inhibitory effect on biofilm formation was measured by the ELISA reader apparatus. The assay was repeated three times, and the average was calculated as 3. The results were compared with those of Chlorhexidine 0.2%. Carum copticum showed a better effect in the agar well diffusion method than others. MIC of the extracts of Carum coptimum, Phlomis bruguieri, and Marrubium parviflorum were 3.12, 6.25, and 12.5 mg/ml, respectively. Overall, the highest activity belonged to Carum copticum extract. For the anti-biofilm effect, the OD values of Carum copticum and Marrubium parviflorum were significantly different from that of Phlomis bruguieri. Although all of the methanolic herbal extracts can inhibit S. mutans growth and remove the biofilm, the effect of Carum copticum was better than Phlomis bruguieri and Marrubium parviflorum. Further studies are recommended to indicate how these extracts perform against the bacteria.

Inhibitory activity of the ethanolic extract of Cyperus Rotundus from the Cajamarca region (Contumazá province) in a standardize strain of Streptococcus mutans (ATCC ® 25175 TM )

The purpose of this study was to determine in vitro the inhibitory activity of the ethanolic extract of Cyperus rotundus (Cajamarca - Contumazá) against a standardize strain of Streptococcus mutans (ATCC®25175™). This study was an experimental in vitro study, which consisted of determining the inhibitory effect of three concentrations of the ethanolic extract of Cyperus rotundus: 250 mg/ml, 500 mg/ml, and 1000 mg/ml against a strains of Streptococcus mutans (ATCC®25175™). Ten tests were performed for each concentration, having 0.12% chlorhexidine as a positive control for Streptococcus mutans plates and 10% DMSO as a negative control. To evaluate the inhibitory effect, the disk diffusion method or Kirby-Bauer test was used, reading the results at 48 hours after initial sowing. None of the three concentrations of the ethanolic extract of Cyperus rotundus demosntrated inhibitory effects on the Streptococcus mutans strain; however, the positive control, chlorhexidine, clearly showed inhibition halos of 14.43 mm ± 1.23 mm after 48 hours of incubation. The ethanolic extract of Cyperus rotundus did not inhibit the growth of Streptococcus mutans. It is recommended to deepen the chemical analysis of the components of this plant and explore other extraction methods to verify its bacteriostatic action versus other oral and non-oral microorganisms.

Benefits of freeze dried arabica coffee peels (Coffea arabica) as an oral antimicrobial

Background: Oral infections are currently treated with synthetic drugs, namely antimicrobials (antibiotics and antifungals) that can cause resistant bacteria, so alternative antimicrobials from herbal groups are needed. Arabica coffee peel contains phytoche-mical compounds (polyphenols, alkaloids, saponins) which have potential as natural antibacterial. Purpose:To examine the benefits of waste of arabica coffee rind in inhibiting growth of Streptococcus mutans, Porphyromonas gingivalis and Candida albicans. Methods: This experimental laboratory with the post test only group design was started with making of freeze dried coffee peels 25%, 50% and 75%; positive control used chlorhexidine and nystatin. The antimicrobial test method was performed by the diffusion method and the growth of microbial colonies was calculated using a colony counter. Result: Antimicrobial effect increased at all concentration of test solutions to the three microbes. This shows that there is a strong positive relationship between concentration and the number of microbial colonies. The higher concentration of dried arabica coffee peels, the greater the effect of microbial killing power. The biggest effect is on C.albicans, P.gingivalis, S.mutans, consecutively. Conclusion: arabica coffee fruit skin can be used as an antimicrobial oral cavity against the growth of S.mutans, P.gingivalis and C.albicans.

Streptococcus mutans and Candida albicans Biofilm Inhibitors Produced by Lactiplantibacillus plantarum CCFM8724.

Lactiplantibacillus plantarum CCFM8724 inhibits the growth of Streptococcus mutans and Candida albicans in mixed-species biofilm formation. In this study, bioactive compound including cyclo (leu-pro), cyclo (phe-pro), and some organic acids, such as 3-phenyllactic acid, hydrocinnamic acid, and palmitic acid, were identified through GC-MS analysis. At 50μg·mL-1, cyclo (leu-pro) reduced biofilm mass (OD600) from 3.00 to 2.00, and hydrocinnamic acid at 25μg·mL-1 reduced biofilm mass (OD600) from 3.00 to 1.00. The expression of ALS3 and HWP1 was downregulated by cyclo (leu-pro). Furthermore, a mixture of cyclo (leu-pro), cyclo (phe-pro), 3-phenyllactic acid, hydrocinnamic acid, and palmitic acid, had anti-biofilm activity. Overall, the results provide promising baseline information for the potential use of this probiotic and its components in preventing biofilm formation.

Nicotinamide could reduce growth and cariogenic virulence of Streptococcus mutans

ABSTRACT Dental caries is among the most prevalent chronic oral infectious diseases. Streptococcus mutans, a major cariogenic bacterial species, possesses several cariogenicity-associated characteristics, including exopolysaccharides (EPS) synthesis, biofilm formation, acidogenicity, and aciduricity. Nicotinamide (NAM), a form of vitamin B3, is a non-toxic, orally available, and inexpensive compound. The present study investigated the inhibitory effects of NAM on the cariogenic virulence factors of S. mutans in vitro and in vivo. NAM inhibited the growth of S. mutans UA159 and the clinical isolates. In addition, there was a decrease in the acid production and acid tolerance ability, as well as biofilm formation and EPS production of S. mutans after NAM treatment. Global gene expression profiling showed that 128 and 58 genes were significantly downregulated and upregulated, respectively, in NAM-treated S. mutans strains. The differentially expressed genes were mainly associated with carbohydrate transport and metabolism, glycolysis, acid tolerance. Moreover, in a rat caries model, NAM significantly reduced the incidence and severity of smooth and sulcal-surface caries in vivo. NAM exhibited good antimicrobial properties against S. mutans, indicating its potential value for antibiofilm and anti-caries applications.

Antibacterial Effects of Steeped White Tea, Black Tea, and Green Tea against Streptococcus mutans and Plaque Accumulation

BACKGROUND: Tea is the most consumed beverage in the world after water. Tea (Camellia sinensis) is native to Southeast Asia and is now available in more than 30 countries. Tea has various health properties. Tea has an active component, namely catechins, which can inhibit the growth of Streptococcus mutans as a component of dental plaque formation. AIM: This study aims to determine the antibacterial effect of steeped white tea, black tea, and green tea on S. mutans and plaque accumulation. METHODS: The research used a quasi-experimental design with pre-test and post-test group design. The population was students of the Department of Dental Nursing. Purposive sampling technique is used with a sample size of 48 students who were divided into three parts of the sample group for the 2.5% concentration of tea mouthwash treatment. The analysis used paired t-test to see the antibacterial effect and differences in three-variable test followed by Kruskal–Wallis test. RESULTS: The results showed that the mouth rinses of steeped white tea, black tea, and green tea were effective in reducing S. mutans and plaque accumulation on teeth. There is a significant difference in the inhibition zone of S. mutans against the three types of tea, and white has the largest inhibition zone. As for plaque accumulation, there is no difference between the three types of tea. CONCLUSIONS: It can be concluded that mouth rinsing with steeping white tea, black tea, and green tea with a concentration of 2.5% has antibacterial properties that can inhibit S. mutans and plaque accumulation.