Mushroom Growth Research Articles

Calcium chloride treatment delays the quality deterioration of king oyster mushroom (Pleurotus eryngii) through positive regulation of antioxidants by PeCaMK1

Calcium (Ca2+) signaling plays a crucial role in the growth, development, and stress responses of mushrooms. However, its specific mechanisms in regulating postharvest quality are not fully understood. This study aimed to investigate the underlying mechanism of CaCl2 treatment on the quality of postharvest Pleurotus eryngii and provide theoretical support for the development of future preservation technology. The study found calcium chloride (CaCl2) treatment to be effective in delaying the browning of P. eryngii, reducing weight loss, and mitigating cell membrane damage. The antioxidant levels, and Ca2+ fluorescence signal was measured, to further examine the physiological effects of CaCl2 treatment. The study found that CaCl2 treatment stimulated the activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POD), ultimately reducing reactive oxygen species (ROS) accumulation. CaCl2 also increased cytolytic Ca2+ levels and the expression levels of Ca2+ signaling genes. The overexpression and silencing transformants were constructed to further analyze the role of PeCaMK1, a gene encoding calcium/calmodulin-dependent protein kinase in Ca2+ signaling. The overexpression of PeCaMK1 increased Ca2+ levels, stimulated the activities of antioxidant enzymes, and inhibited ROS accumulation. The reduced expression of PeCaMK1 by RNAi-mediated gene silencing increased ROS level, demonstrating that PeCaMK1 positively regulated the antioxidant effect in response to Ca2+ signaling. In summary, the results suggested that exogenous CaCl2 treatment transmitted Ca2+ signaling through PeCaMK1 to enhance antioxidant activity, reduce ROS accumulation, and ultimately delay the quality deterioration of P. eryngii.

Altering Microbial Communities in Substrate to Stimulate the Growth of Healthy Button Mushrooms

Green mould, caused by Trichoderma aggressivum, is one of the major fungal diseases of button mushrooms. The main problems in chemical disease control include a lack of effective agents, the occurrence of pathogen resistance to pesticides, and the harmful impact on the environment. In an attempt to find a solution, the interaction between two beneficial microorganisms, Bacillus amyloliquefaciens B-241 (an antifungal agent) and Streptomyces flavovirens A06 (a yield stimulant), was investigated in vivo. The synergy factor (SF) was calculated as a ratio between the observed and expected impact on the yield or efficacy of disease suppression after artificial inoculation with T. aggressivum. The highest control of T. aggressivum was achieved by joint application of the two beneficial microorganisms. The additive interaction between microorganisms in efficacy against the pathogen was revealed. The largest yield was obtained in mushroom beds sprayed with the two beneficial microorganisms combined (B-241 80% and A06 20%). Regarding the impact on the yield, synergistic interaction between the two microorganisms was confirmed (SFs were 1.62 or 1.52). The introduction of optimized microbial combinations could create new possibilities for biorational edible mushroom protection, with improved yield and quality and reduced risks to human health and the environment.

Effect of Differences in the Composition of Growing Media on the Growth of White Oyster Mushroom Mycelium (Pleurotus ostreatus)

The use of the main growth medium in the form of wood sawdust in mushroom domestication is still higher than that of other organic materials, even though its availability is limited, so efforts need to be made to find a growth medium composition that minimizes the percentage of sawdust use. The purpose of this study was to determine the comparison of treatment 1 (80% teak sawdust, 10% bran, 5% tapioca flour, and 5% agricultural lime) with treatment 2 (70% teak sawdust, 15% bran, 10% tapioca flour, and 5% agricultural lime) on the percentage of growth and mycelium growth of white oyster mushrooms. Composting of growing media was carried out for 5 days, then baglog making was carried out, after which the baglog was sterilized for 4 hours. After sterilization, the baglog was cooled and inoculated. After inoculation, the baglog is incubated to grow mycelium. Then, after 15 days, data collection and calculation were carried out. The parameters measured were the percentage of growth and mycelium growth of white oyster mushrooms in baglogs. Results showed that the percentage of growth and mycelial growth of white oyster mushrooms in treatment 1 is better than in treatment 2. Reducing the percentage of the main growing medium of teak sawdust and adding the percentage of additional growing media of bran and tapioca flour has a negative impact on the number of baglogs that grow mycelium (percentage of growth) and the growth of white oyster mushroom mycelium. This means that reducing the main media of teak sawdust and adding additional media is not the right growing media composition for domestication of white oyster mushrooms, so it cannot be used as an alternative growing media composition to reduce the use of wood sawdust.

In vitro Assessment of Botanical Compatibility with Agaricus bisporus for Sustainable Disease Management

Button mushroom (Agaricus bisporus) cultivation is a significant aspect of the global mushroom industry, the production of fruiting bodies is severely affected by fungal, bacterial and viral pathogens that can have an effect on yield and quality. Botanicals, such as plant extracts, have been reported to possess various bioactive compounds with potential benefits for mushroom growth and disease control. A lab experiment was carried out in the Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, to evaluate the compatibility of various botanicals viz., Allium sativum (garlic), Coriandrum sativum (coriander), Cuminum cyminum (jeera), Mentha spicata (pudina) with Agaricus bisporus in a Completely Randomised Design. In vitro evaluation was carried out using poison food technique at 10, 20 and 30% concentrations and 24, 48 & 72 hours of incubation. The maximum radial growth of Agaricus bisporus was observed in T0-Control (Agaricus bisporus) (17.86 mm), (16.82 mm) and (16.53 mm) and minimum in T3- Cuminum cyminum (jeera) (2.23 mm), (0.98 mm) and (0.39 mm) at 10, 20 and 30 percent concentration respectively. Maximum percentage of inhibition was observed in T3 - Cuminum cyminum (jeera) (87.51 %), (94.11 %) and (98.24 %) at 10, 20 and 30 percent concentration respectively.

Mushrooms grown in high-altitude soil exhibiting distinct alterations in growth, biochemical composition and antioxidant potential

Functional foods like mushrooms are high in secondary metabolites and bioactive chemicals with anti-inflammatory, antibacterial, antidiabetic, immune-stimulating and other health-promoting qualities. This study investigates four mushroom varieties including Cordyceps militaris, Pleurotus ostreatus, Pleurotus djamor and Agaricus bisporus, grown on Indian soil and natural environment for measurement of phenol and flavonoid content, along with their antioxidant properties and biochemical makeup. They had a large amount of phenolic and flavonoid content and high DPPH, H2O2 and NO scavenging inhibition percentages, demonstrating strong antioxidant potential. These mushroom extracts also contained a sizable amount of proline and nutritional content like carbohydrate, protein, lipids and fiber. Among all the mushrooms, the biochemical profile was significantly highest. Significant variations were also seen in the amounts of moisture and ash in each sample. This research aimed to determine how mixing soil with compost substrate affected the yield and growth of mushrooms. The soil had a massive impact on forming more mycelial colonies which were necessary for the entire spawn run and spawning to pin head production and for the maturation of pins. It was discovered through this research that combining soil and compost substrate can aid in the growth of good-quality mushrooms and improve their productivity.

Changes of Active Substances in Ganoderma lucidum during Different Growth Periods and Analysis of Their Molecular Mechanism.

Ganoderma lucidum, renowned as an essential edible and medicinal mushroom in China, remains shrouded in limited understanding concerning the intrinsic mechanisms governing the accumulation of active components and potential protein expression across its diverse developmental stages. Accordingly, this study employed a meticulous integration of metabolomics and proteomics techniques to scrutinize the dynamic alterations in metabolite accumulation and protein expression in G. lucidum throughout its growth phases. The metabolomics analysis unveiled elevated levels of triterpenoids, steroids, and polyphenolic compounds during the budding stage (BS) of mushroom growth, with prominent compounds including Diplazium and Ganoderenic acids E, H, and I, alongside key steroids such as cholesterol and 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol. Additionally, nutrients such as polysaccharides, flavonoids, and purines exhibited heightened presence during the maturation stage (FS) of ascospores. Proteomic scrutiny demonstrated the modulation of triterpenoid synthesis by the CYP450, HMGR, HMGS, and ERG protein families, all exhibiting a decline as G. lucidum progressed, except for the ARE family, which displayed an upward trajectory. Therefore, BS is recommended as the best harvesting period for G. lucidum. This investigation contributes novel insights into the holistic exploitation of G. lucidum.

Optimalisasi Inovasi Produk Makanan Berbahan Dasar Jamur sebagai Upaya Penguatan Wanita Tani Jamur melalui Pelatihan dan Pendampingan

Genting Village, Semarang, has great potential for mushroom cultivation thanks to geographical and climatic conditions that favour healthy mushroom growth. Although farm women have been actively involved in mushroom cultivation in this village, they often face obstacles in terms of access to resources, knowledge, and marketing of mushroom-based processed food products. To overcome this problem, we, UKM Hizbul Wathan, in the PPK Ormawa 2023 grant activity, propose training and mentoring in the manufacture and marketing of mushroom-based food in Genting Village. The method we use is SWOT analysis, used to identify strengths, weaknesses, threats, and development strategies in overcoming the problems of the mushroom farmer women's group in Genting Village. Strengths include favourable geographical conditions for mushroom cultivation, participation of women farmers, and unique local wisdom. Meanwhile, the main weaknesses are limited access to resources and knowledge. Opportunities in diversification of mushroom-based food products and increasing consumer awareness about healthy food should be utilised. Threats include market competition and climate change. The discussion focuses on strategies for developing training programmes to improve farm women's knowledge and skills, effective marketing efforts, and partnership opportunities with external parties. The results of this study show that mentoring and training in mushroom-based food processing can create new economic opportunities, increase the income of farm women, and support sustainable development in Genting Village.

The Effect of Adding Tea Dregs on The Growth of Brown Oyster Fungus (Pleurotus cystidiosus) on Sawdust Planting Media

The aim of the research was to determine the effect of adding tea dregs on the growth and the effective composition of tea dregs on the growth of brown oyster mushrooms (Pleurotus cystidiosus) on sawdust planting media. The type of research is quantitative with an experimental method using a Non-Factoral Completely Randomized Design (CRD) with 5 treatments and 4 replications. Data analysis in the study used parametric tests, namely the Anova test and LSD test as well as non-parametric tests, namely the Kruskal Wallis test and the Mann-Whitney test with SPSS. The results showed a significant effect on mycelium growth (Sig 0.014), number of fruit bodies (Sig 0.016), fruit stalk length (Sig 0.017), wet weight of harvest (Sig 0.000) and a significant effect on the age at which fruit bodies emerged (Sig 0.045) and had no significant on the diameter of the fruit cap (Sig 0.088) of brown oyster mushroom (Pleurotus cystidiosus). The best treatment for each observation parameter was mycelium growth in treatment P4 (19.3938 cm), age of emergence fruit bodies in treatment P0 (34.5 days), number of fruit bodies in treatment P4 (5.375), diameter of fruit cap in treatment P0 (12.3 cm), fruit stalk length in treatment P4 (10 cm), and wet harvest weight in treatment P4 (91 grams). The most effective treatment for the growth of brown oyster mushrooms ((Pleurotus cystidiosus) is P4 treatment with 25% (375 grams of Tea Dregs + 75% (1,125 grams of Swadust).

Pemberdayaan Kelompok Tani Kana Melalui Pelatihan Budidaya Jamur Tiram Berbasis Pemanfaatan Limbah Furniture

This community service activity aims to increase the knowledge and skills of the Kana farmer group in cultivating oyster mushrooms by utilizing furniture waste. The method of implementing this service uses participatory action research with the stages of planning, implementation, monitoring, and evaluation. The evaluation instrument for this activity uses a questionnaire sheet, and then the data obtained is analyzed descriptively. The results of this service show that there is an increase in knowledge about the utilization of furniture waste in oyster mushroom growth media and provide skill experience to farmer groups in cultivating oyster mushrooms.

Pre- and Postharvest Strategies for Pleurotus ostreatus Mushroom in a Circular Economy Approach.

Mushroom cultivation presents a viable solution for utilizing agro-industrial byproducts as substrates for growth. This process enables the transformation of low-economic-value waste into nutritional foods. Enhancing the yield and quality of preharvest edible mushrooms, along with effectively preserving postharvest mushrooms, stands as a significant challenge in advancing the industry. Implementing pre- and postharvest strategies for Pleurotus ostreatus (Jacq.) P. Kumm (oyster mushroom) within a circular economy framework involves optimizing resource use, minimizing waste, and creating a sustainable and environmentally friendly production system. This review aimed to analyze the development and innovation of the different themes and trends by bibliometric analysis with a critical literature review. Furthermore, this review outlines the cultivation techniques for Pleurotus ostreatus, encompassing preharvest steps such as spawn production, substrate preparation, and the entire mushroom growth process, which includes substrate colonization, fruiting, harvesting, and, finally, the postharvest. While novel methodologies are being explored for maintaining quality and extending shelf-life, the evaluation of the environmental impact of the entire mushroom production to identify areas for improvement is needed. By integrating this knowledge, strategies can be developed for a more sustainable and circular approach to Pleurotus ostreatus mushroom cultivation, promoting environmental stewardship and long-term viability in this industry.

Assessment of Various Substrates for Shiitake Mushroom (Lentinula edodes) Cultivation in the Agro-Climatic Conditions of West Bengal

Aim: This study aimed to evaluate the impact of different substrates on various stages of shiitake mushroom (Lentinula edodes) cultivation to optimize growth and yield. Study Design: A Completely Randomized Design with three replications for each treatment was employed to ensure statistical reliability. Duncan's multiple range tests were utilized at a significance level of 5% to evaluate differences between treatments. Place and Duration of Study: The research was conducted at the Mushroom Research Laboratory, Department of Plant Pathology, Palli Siksha Bhavana (Institute of Agriculture), located in Sriniketan, Birbhum district, West Bengal, throughout the year 2023. Methodology: Different substrates including lentil husks, rice straw, wheat straw, sawdust, and mustard pods were evaluated for their impact on spawn run completion, mycelial bump formation, and sporocarp formation durations. Each substrate's performance was assessed in terms of sporocarp production and biological efficiency. Results: Across the three strains (LE-01, LE-02, and LE-03), lentil husks consistently exhibited the shortest spawn run duration, averaging 34.59 to 37.00 days. Following closely, wheat straw displayed favorable performance with durations ranging from 39.20 to 41.23 days. Sawdust also proved efficient, albeit slightly longer than lentil husks and wheat straw. Conversely, maize cobs and sugarcane bagasse exhibited longer spawn run durations, averaging from 57.80 to 64.90 days. For mycelial bump formation, lentil husks again showed the shortest duration, averaging 42.67 to 45.77 days across the strains. Wheat straw followed suit with durations ranging from 48.93 to 50.20 days, while sawdust demonstrated favorable times albeit slightly longer. Conversely, maize cobs and sugarcane bagasse exhibited longer mycelial bump formation times, ranging from 68.11 to 74.40 days. For sporocarp formation, lentil husks and sawdust emerged as top performers with the shortest durations, averaging 76.66 to 81.52 days. Conversely, maize cobs exhibited the longest sporocarp formation time, averaging 97.43 days. In terms of sporocarp yield, rice straw yielded the highest, averaging 27.67 sporocarps per bag, followed by wheat straw with 24.33 sporocarps per bag. Lentil husks, while efficient in other aspects, produced an average of 21.78 sporocarps per bag, trailing slightly behind rice straw and wheat straw. Regarding yield and biological efficiency, sawdust displayed superior performance, yielding an average of 384.57 grams per 750 grams of substrate with a biological efficiency of 51.18%. Lentil husks and rice straw exhibited comparable performance in yield and biological efficiency, with lentil husks yielding 367.81 grams and rice straw yielding 333.09 grams per 750 grams of substrate, both with biological efficiencies around 47-48%. Conversely, mustard pods exhibited the lowest yield and biological efficiency, averaging 269.02 grams per 750 grams of substrate with a biological efficiency of 35.91%. Conclusion: The study underscores the importance of substrate selection in optimizing various stages of L. edodes cultivation. Lentil husks emerged as a promising substrate choice for rapid mycelial growth, but its sporocarp production lagged behind other substrates. Sawdust proved to be the top-performing substrate in terms of yield and biological efficiency, indicating its efficacy in supporting robust mushroom growth.

Time Aware Long Short-Term Memory and Kronecker Gated Intelligent Transportation for Smart Car Parking

Technology desires to improve quality of life and impart citizen’s health as well as happiness. The concept of Internet of Things (IoT) refers to smart world where prevailing objects are said to be embedded and hence interact with each other (i.e., between objects and human beings) to achieve an objective. In the period of IoT as well as smart city, there is requirement for Intelligent Transport System-based (ITS) ingenious smart parking or car parking space prediction (CPSP) for more feasible cities. With the increase in population and mushroom growth in vehicles are bringing about several distinct economic as well as environmental issues. One of pivotal ones is optimal parking space identification. To address on this problem, in this work, Time-aware Long Short-Term Memory and Kronecker product Gated Recurrent Unit (TLSTM-KGRU) for smart parking in internet of transportation things is proposed. The TLSTM-KGRU method is split into two sections. In the first section, smart parking occupancy is derived using Time-aware Long Short-Term Memory (Time-aware LSTM) for Kuala Lumpur Convention Centre car parking sensor dataset. Following which the resultant smart car occupancy results are subjected to Linear Interpolations and Kronecker product Gated Recurrent Unit for predicting smart parking. When compared against other predictive methods such as SGRU-LSTM and CPSP using DELM, our experimental outcomes denote that TLSTM-KGRU method has improved performance for smart parking occupancy forecast as it not only enhances sensitivity and specificity but also reduces prediction time with minimum delay.

Detection and Measurement System for Button Mushrooms Using Convolutional Neural Network

In Malaysia, the button mushroom is recognized as a vegetable with high nutritional value and is easy to cultivate. Monitoring mushroom growth requires farmers to regularly inspect their crops, which is time-consuming and inefficient. Hence, an automated detection and measurement system for button mushrooms has been developed using image processing techniques based on convolutional neural network (CNN) algorithm model known as YOLOv4. The algorithm was utilized to train the system using button mushroom images to create training models. The performance of the YOLOv4 models was evaluated across various iterations ranging from 1000 to 6000 iterations. The model with 2000 iterations demonstrated the most effective performance based on Recall, Precision, F1-score, Time and Mean Average Precision metrics. The model was used in a small-scale experimental setup to evaluate the button mushroom detection and measurement system’s performance. Based on the results obtained from the experiments, the detection and measurement system demonstrated high accuracy in locating the position of each button mushroom with only a 5% deviation error in predicting the size of each button mushroom.

Harnessing Essential Oils for in-vitro Control of Trichoderma sp. in Mushroom Cultivation

Mushrooms are highly nutritious dietary items that are rich in vitamins, minerals, antioxidants, and high-quality protein. Oyster and button mushrooms are highly significant species, cultivated in diverse farms and locations. Various fungal contaminants, including Trichoderma sp. hindered the mycelium growth of button mushroom, hence reduces its yield. In this study, bio-management of Trichoderma horzianum which is a major causative agent of mushroom mold was investigated by using different essential oils viz., cinnamon, coconut, neem and rose oil with increasing concentration of 4%, 8%, 12% and 16% against Trichoderma sp. All of the essential oils showed significant impact, but rose oil with concentration of 16% demonstrated the highest efficacy in controlling Trichoderma sp against mushroom mold. Thus, it is suggested that increasing concentration of rose oil would be more effective under in-vitro conditions in controlling Trichoderma horzianum

Optimization of Growth and Production of Brown Oyster Mushrooms (Pleurotus cystidiosus) through Additional Nutrients of Rice Washing Water and Rice Groats

This study aims to determine the optimization of growth and production of brown oyster mushroom (P. cystidionsus) plants with additional nutrients of rice washing water and to determine the optimization of growth and production of chocolate oyster mushroom (P. cystidionsus) plants through additional nutrients of rice groats. This study used a factorial Complete Randomized Design (RAL) method consisting of two factors, namely, the first factor is rice washing water / leri water (L) and the second factor is rice groats (G). Each treatment is repeated 3 times. Leri water factor consists of 4 levels, namely: A0=0 ml, A1= 30 ml, A2= 50 ml, A3=70 ml. Rice groats factor consists of 4 levels, namely: M0=0 g, M1=60 g, M2= 80 g, M3= 100 g. Data was analyzed using variance analysis (Anova) at 5%. The results showed no effect of rice washing water and rice groats on the growth rate of brown oyster mushroom mycelim, will be at a rice washing water concentration of 70ml / baglog + rice groats 100g / baglog affects the parameters of mushroom stalk height (cm), mushroom hood diameter (cm), mushroom hood thickness (cm), number of mushrooms (clump), and fresh weight of mushrooms / baglog (g).

Growth and Production Stimulation of Young Pink Oyster Mushrooms (Pleurotus flabellatus) through CaCO3 Supplementation from Shellfish Shells and Coconut Water

Pink oyster mushroom (Pleurotus flabellatus) is one of the oyster mushroom varieties that has a distinctive aroma similar to lingzhi mushroom, pink color, numerous clusters, and a slightly firm texture. The aim of this research was to determine the growth and production stimulation of pink oyster mushrooms (P. flabellatus) through supplementation of CaCO3 from shellfish shells and coconut water. This study utilized a Completely Randomized Design (CRD) arranged in factorial with two factors, consisting of four levels with three replications. The first factor was CaCO3 supplementation from shellfish shells with four levels: K0: 0 gr/baglog, K1: 5 gr CaCO3 from shellfish shells/baglog, K2: 10 gr CaCO3 from shellfish shells/baglog, K3: 15 gr CaCO3 from shellfish shells/baglog. The second factor was coconut water supplementation with four levels: A0: 0 ml/baglog, A1: 20 ml coconut water/baglog, A2: 40 ml coconut water/baglog, A3: 60 ml coconut water/baglog. The results showed significant differences in mycelium growth rate and highly significant differences in pileus number, pileus width, pileus thickness, stalk height, and mushroom fresh weight. Supplementation of 10 gr CaCO3 from shellfish shells/baglog and 40 ml coconut water/baglog showed the best interaction for all parameters.

THE EFFECT OF USING SAGU DRUGS (Metroxylon sagu) AND DRIED BANANA LEAVES (Musa paradisiaca) ON THE GROWTH OF WHITE OYSTER MUSHROOM (Pleurotus ostreatus)

Oyster mushrooms (Pleurotus ostreatus) are wood fungi that grow sideways on rotting wood stems. Mushrooms take food that has been made by other dead organisms (saprophyte), because they do not have chlorophyll, all types of saprophyte, especially those that grow on wood, can be easily cultivated. White oyster mushroom cultivation is easy to do because it does not require expensive maintenance costs and does not require spacious land. The aim of this research was to test the effect of using sago dregs and dried banana leaves on the growth of white oyster mushrooms. The results of this research indicate that there are differences in the growth of white oyster mushrooms based on different concentrations of growing media. The average mycelium growth, stem height, pileus diameter, number of fruit bodies, wet weight and dry weight of the best white oyster mushrooms were found in media with a concentration of 100% sawdust, while pind heads appeared in a concentration of 100% sago dregs

Effect of Different LED Light Wavelengths on Production and Quality of Pleurotus ostreatus Grown on Different Commercial Substrates

Artificial lighting, primarily employed in crop production, can also be applied to the cultivation of edible mushrooms to enhance productivity and quality. While UV radiation has predominantly been investigated in post-harvest treatments for edible mushrooms, the utilization of different light wavelengths during the cultivation phase remains largely unexplored for many mushroom species. This study aimed to assess the impact of three different light wavelengths 450 nm (B), 610 nm (R), and a combination of these two wavelengths (R + B) on the productive characteristics and quality of Pleurotus ostreatus, cultivated using three straw-based commercial substrates. It was observed that, except for yield, artificial light influenced mushroom growth. Specifically, the application of R light appeared to promote mycelium growth, whereas B light contributed to increase the diameter of fruiting bodies. Additionally, the concentration of vitamin D2 was higher under both B and R+B light treatments. Interestingly, the light treatments did not affect yield but impacted diameter and various chemical attributes such as EC, total soluble solids, and titratable acidity. In conclusion, exposure to different lighting affected Pleurotus ostreatus physiology and nutritional content.

Effect of Bacillus aryabhattai extract addition on growth and productivity of Pleurotus ostreatus ((Jacq ex Fr.) P. Kumm)

Pleurotus ostreatus (White oyster mushroom) is one of the most widely cultivated mushrooms in the world. Nutrients provided in the media are one of the most important factors affecting the growth of mushrooms. The present study mainly focuses on the isolation of mushroom growth-promoting (MGP) bacteria and studies its effects on the growth of mushroom fruiting bodies. Bacillus aryabhattai was isolated from the growth media of Pleurotus ostreatus, identified based on 16S rDNA sequencing and phylogenetic tree construction by the neighbor-joining method using MEGA version 6.06 and its effect on fruiting body growth of the P. ostreatus was investigated. The results of this research strongly suggest that the addition of specific bacteria supernatant into the mushroom growth media has beneficial applications for mushroom production.

EFFICACY OF ESSENTIAL OILS AGAINST Penecillium sp. CAUSING MUSHROOM MOLD UNDER IN VITRO CONDITION

Mushrooms are nutritive and medicinal foods; Oyster and button are the very important mushrooms being grown at various farms, localities etc. Varieties of fungal contaminants limit the mycelium growth of the button mushroom as well as others in the substrate and affecting its yield. In this study, it was determined the Penecillium sp is the major mold affecting the yield of the Oyster mushroom. Furthermore In-Vitro experiment was carried out for management of mushroom against Penecillium sp. Essential oils viz., cinnamon oil, coconut oil, Neem oil and rose oil were used at various concentrations (4%, 8%, 12% and 16%) against Penecillium sp. All the essential oils showed impressive results, among all treatments Neem oil showed high reduction of colony growth of Penecillium sp. followed by coconut oil, Rose oil and Cinnamon oil. All the data was statically analyzed.