Growth Hormone Gene Research Articles

Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail.

This experiment was conducted to evaluate the effects of dietary supplementation of whey powder (WP), Bacillus subtilis (BAS), and their combination (MIX) on growth performance, intestinal morphology, caecal microflora, hepatic gene expression, blood metabolites, and skeletal muscle antioxidant capacity in Japanese quails. A total of 400 one-day-old Japanese quails were randomly distributed to 20-floor pens (4 dietary treatments, 5 replications per treatment, 20 birds per pen). The birds were fed a basal diet (control, CON) or the basal diet supplemented with 40g/kg WP, 1g/kg BAS probiotic or 40g/kg WP plus 1g/kg BAS probiotic for five weeks. Feed intake was not affected by the treatments at any stage of the trial. However, the WP, BAS, and MIX feed had better weight gain and feed conversion ratio compared to the CON during the entire production period (day 1-35; p<.05). Feeding the WP, BAS, and MIX diets caused no significant difference in morphometric measures in the duodenum, jejunum, and ileum other than the villus height to crypt depth ratio in the ileum (p<.05). The expression of insulin-like growth factor-1 (IGF-1) and growth hormone genes was highly upregulated in the liver of the birds fed the MIX diet (p<.05). Feeding birds with the diets containing WP, BAS, and MIX increased the population of caecal lactic acid bacteria and reduced serum cholesterol concentration compared to the CON diet (p<.05). Likewise, the tested feed additives increased superoxide dismutase and glutathione peroxidase enzyme activities in the thigh muscle (p<.05). No synergistic effect was found between WP and BAS in studied parameters other than IGF-1 gene expression. Improved growth performance of Japanese quails by feeding the WP, BAS, and the MIX feed could be linked to improved absorptive capacity of the small intestine as well as over-expression of anabolic growth factors. In conclusion, WP with or without BAS could be considered as a beneficial dietary supplement to enhance productive performance, gut functionality, and antioxidant capacity of Japanese quail.

Effect of growth hormone gene polymorphism on growth traits in migratory Gaddi goats of Western Himalayas, India.

Goat production under migratory system is foremost meat resource in Western Himalayan region of India. Thus, selection of goats for superior growth rate is rewarding. Growth hormone (GH) gene is identified as main regulator of post-natal growth and development. The objective of this study was to identify GH gene variants in Gaddi goats reared under migratory system via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-strand conformation polymorphism (SSCP). Blood samples from 63 animals from different migratory flocks registered under All India Coordinated Research Project, Himachal Pradesh Agricultural University (HPAU), Palampur, were subjected to DNA isolation. A total of 422, 116, 389 and 181-bp amplicons were generated on amplification of four targeted regions of GH gene. GH1 and GH2 fragments were analysed using PCR-RFLP (HaeIII RE) that revealed three variants (AA, AB and BB) for GH1 having frequency as 0.27, 0.52 and 0.31, respectively whereas, two variants (AB and BB) were revealed for GH2 fragment having frequency of 0.24 and 076, respectively. GH3 and GH4 fragments were subjected to PCR-SSCP that detected three genotypes (AB, BB and AA) for GH3 with respective genotype frequency as 0.57, 0.21 and 0.22 respectively; however, GH4 was found to be monomorphic. The polymorphism information content values for GH1, GH2 and GH3 were 0.37, 0.36 and 0.34, respectively, which suggested the median level of polymorphism at studied loci and also indicated the effectiveness of the studied marker for population genetic studies. Significant associations (P ≤ 0.05) were detected for GH1 with 9-month body weight, GH2 with 9 and 12-month heart girth and GH3 with 6-month body weight, body height and body length, respectively. From the present study, it was concluded that SNPs and their association with some body measurements may be employed as useful markers for ongoing phenotypic selection programme.

Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle

Abstract. Hartatik T., Fathoni A, Bintara S., Ismaya, Panjono, Widyobroto B.P, Agus A, Budisatria I.G.S, Leroy P. 2020. Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle. Biodiversitas 21: 941-945. Growth Hormone gene has been proposed to direct search for quantitative trait loci, and polymorphisms at these loci have been associated with several production traits in bovine. This research aimed to investigate the association of the genotype of partial growth hormone gene with birth weight and average daily gain in crossbred beef cattle. The materials of this research consist of 47 samples from four different breeds. Birth weight was measured just after calving delivery from the mother. The average daily gain was calculated from the period since birth weight to the second weight (90 days). Genotyping of the growth hormone gene was determined by the sequencing and PCR-RFLP method with the AluI restriction enzyme. The association between genotype and growth traits data was analyzed using analysis of variance (ANOVA) by RStudio version 1.1.383. The results showed the significantly different (P&lt;0.001) in birth weight and average daily gain according to the different breed. Friesian Holstein breed showed the highest birth weight, followed by Belgian Blue Bull cross and others. The genotype valine/valine only occurs in Belgian blue bull cross and Wagyu bull cross cattle and showed moderate birth weight. Genotype affects birth weight significantly (P&lt;0.001). The birth weight for genotype leucine/leucine, leucine/valine, and valine/valine was 34.82±18.13, 25.31±13.10, and 29.35±13.65, respectively. There was no significant difference in average daily genes according to the different genotypes. In conclusion, the growth hormone gene was probably one of the molecular genetics markers for excellent growth traits in different crossbred beef cattle.

Genotype-environment interactions for survival and growth rate at varying levels of sodium chloride for growth hormone transgenic channel catfish (Ictalurus punctatus), channel catfish, and albino channel catfish

Lack of freshwater is emerging as the most critical natural resource issue facing humanity. Ongoing climate change will reduce freshwater supplies, and demand for food continues to expand rapidly. Swim-up channel catfish, Ictalurus punctatus, fry transgenic for the channel catfish growth hormone (GH) gene driven by the rainbow trout Oncorhynchus mykiss metallothionein promoter (rtMT-ccGH), or by the ocean pout Zoarces americanus antifreeze protein promoter (opAFP-ccGH), channel catfish, and albino channel catfish were grown at 0, 2.5, 5, and 7.5 parts per thousand (ppt) salinity. Survival was 100% for all genetic groups at 0 and 2.5 ppt. Increasing salinity to 5 ppt decreased overall survival as survival rates of rtMT-ccGH transgenic (T), rtMT-ccGH control (C), opAFP-ccGH transgenic (T), opAFP-ccGH control (C), channel catfish, and albino channel catfish were 83, 82, 83, 77, 89 and 40%, respectively. Increasing salinity to 7.5 ppt had a strong negative impact on survival as means for rtMT-ccGH (T), rtMT-ccGH (C), opAFP-ccGH (T), opAFP-ccGH (C), channel catfish, and albino channel catfish were 67, 13, 67, 10, 18, and 7%, respectively with the albino channel catfish having the lowest survival followed by opAFP-ccGH (C) (P = .002). Raising salinity to 2.5 ppt greatly increased the growth rate of GH transgenic channel catfish (11–33%), channel catfish (56%), and albino channel catfish (124%). NaCl had a negative effect on survival and growth rate for swim-up fry at 5 ppt. Significant differences were observed at varying salinity (P < .0001) for final body weights among genetic groups. Condition factor, growth and survival were not perfectly correlated, and genotype-environment interactions occurred for the overall phenotype. Apparently, GH can play a critical positive role for channel catfish under osmotic stress, which has relevance for aquaculture management under future global climate change. Optimum performance for all genotypes was observed with slightly elevated, 2.5 ppt. Although, GH transgenic catfish were less affected than non-transgenic channel catfish at 5–7.5 ppt, their performance was sub-optimal. GH transgenic fish are beneficial for use in culture environments that have slight shifts in salinity in the future, but they are only a partial solution for future environments at 5 ppt and higher.

Evidence for divergence between sympatric stone charr and Dolly Varden along unique environmental gradients in Kamchatka

Here, we describe the biological and ecological differences between partially anadromous Dolly Varden and riverine stone charr distributed in the Kamchatka River middle course. Endemic stone charr, being the ambush predator, is defined by the accelerated growth and prolonged lifespan, robust body, and large mouth, as well as specific marble coloration since an early age. The significant restriction in gene flow between the stone charr and benthos-eating Dolly Varden was supported by an allelic distribution of eight microsatellite loci. Herewith, the identity of mitochondrial DNA control region and intron sequences of growth hormone and metallothionein genes confirms a relatively recent diversification of the groups within the single basin. We suggest the key role of the specific environment for reproduction isolation of stone charr. It occupies coniferous taiga zone with dark-colored water, whereas Dolly Varden spawns uppercourse in the zone of stunted, windblown forest. Conifer litter decomposition products could drive natural selection of stone charr via metabolism acceleration and resistance to toxicants. The metabolic difference could promote piscivory and development of the specific morphological peculiarities of the stone charr. Therefore, we present the first evidence in favor of trophic-based sympatric diversification in a river basin at high latitudes.

Identifikasi Keragaman Gen TGF-β2 dan Asosiasinya dengan Sifat Pertumbuhan pada Ayam Tolaki

The transforming growth factor-beta 2 (TGF-β2) gene is a group of growth hormone (GH) genes that control the nature of growth and belongs to the cytokine group. This study aimed to identify Single Nucleotide Polymorphism (SNP) g.640 T&gt;C from the TGF-β2 gene then associated with the growth properties of tolaki chickens. Chicken blood samples used in this study amounted to 70 birds. Data on the nature of growth observed include; hatched weight, feed consumption, body weight, body weight gain and feed conversion. The TGF-β2 gene polymorphism was identified using the PCR-RFLP method. SNP g.640 T&gt;C in the TGF-β2 gene was polymorphic and wasin Hardy-Weinberg balance. Identification results found 2 alleles, namely T and C and 3 genotype namely TT, TC, and CC. The association of TGF-β2 gene diversity in tolaki chicken generally showed a difference (P&lt;0.05) on feed consumption, body weight and body weight gain. CC genotype has higher feed consumption, body weight, weight gain and more efficient feed conversion than TC and TT genotypes.

English

Many factors contribute to the underdevelopment of oceanic aquaculture. The most significant factor of the development of hybrid grouper culture is the supply of satisfactory quantities of fast growing juveniles to stock grow-out systems at a minimized cost. In order to evaluate the growth hormone status of hybrid grouper (Epinephelus fuscoguttatus ♀&times; Epinephelus polyphekadion ♂), cDNA encoding growth hormone from the liver of juvenile fish was cloned and characterized. qPCR method was used to determine the expression of the growth hormone in the tissues. Relative expression of growth hormone in the brain was found to be highest (290.91 folds) and lowest in the stomach (1.35 folds). Bioinformatics tools were used to analyze the growth hormone proteins and prediction of its physicochemical properties, and amino acid compositions, as well as Gene Ontology, GO term predictions. The cloned hybrid grouper growth hormone gene sequence was found to be clustered monophyletically with solid bootstrap backing. It was found to be close to the Cromileptes altivelis growth hormone gene sequence and is positioned in the identical clade and consequential from the identical family. The expression pattern is comparable to that comprehended in other fishes and provides extra data for molecular biological studies on hybrid grouper fish. Key words: Growth hormone, bioinformatics analysis, tissue expression, gene ontology, hybrid grouper &nbsp;

Meta Analysis of Allele and Genotype Frequency of Growth Hormone (bGH) Gene AluI Polymorphism, Which is Effective on Milk Yield in Holstein Cattle

Meta Analysis of Allele and Genotype Frequency of Growth Hormone (bGH) Gene AluI Polymorphism, Which is Effective on Milk Yield in Holstein Cattle

ПОЛИМОРФИЗМ ГЕНА СОМАТОТРОПНОГО ГОРМОНА В СВЯЗИ С КАЧЕСТВОМ ТУШ МЯСНОГО СКОТА

Abstract—Meat productivity and meat quality are determined by both paratypical and genetic factors. In this regard, investigating the genetic material for the presence of desirable allele combinations of genes associated with growth and development traits, as well as meat qualities of animals, has scientific and practical significance. The aim of this study was to determine the relationship between growth hormone gene polymorphism and beef cattle carcass quality. A scientific-economic experiment was carried out in the period from 2014 to 2017. Fattening bull-calves of the Hereford (115 animals) and Limousin (114 animals) breeds were genotyped using a polymerase chain reaction followed by subsequent analysis of restriction fragment length polymorphism (SNP GH-L127V). It was revealed that the studied bull-calves have a similar distribution of genotypes; the homozygous genotype GHLL (47.83 and 52.63%) and allele GHL (0.69 and 0.71%) were the most common. It was found that, compared to GHVV bull-calves, GHLL bull-calves had statistically significantly higher indicators of preslaughter live weight, carcass meat weight, slaughter weight, and loin-eye area; in Hereford cattle, slaughter yield was also higher. The results of examining the morphological composition of half-carcasses proved the high impact of SNP GH-L127V on the chilled carcass weight and the meat content. These figures were statistically significantly higher for half-carcasses of GHLL and GHLV bull-calves compared to GHVV animals. Analysis of the weight and yield of natural anatomical parts of half-carcasses obtained from Hereford and Limousin bull-calves with different genotypes did not reveal a significant difference between the indicators. However, for both breeds, animals with genotype GHLL had higher chilled half-carcass weight and weight of their natural anatomical parts than animals with genotype GHVV. Thus, SNP GH-L127V genotyping can be used as an additional criterion in breeding and selection of animals to improve meat qualities of cattle.

Prolactin gene polymorphisms and associations with reproductive traits in Indonesian local ducks.

Background and Aim:Reproductive traits play an important role in population increases and the egg production (EP) abilities of Indonesian local ducks (ILD). The prolactin (PRL) gene is a single chain polypeptide hormone belonging to a family of growth hormone genes that are mainly synthesized in the anterior pituitary gland in all vertebrates. It has a significant effect on reproductive traits and EP. Single nucleotide polymorphisms (SNPs) present in PRL are a useful molecular marker for EP. This study aimed to identify the PRL polymorphisms based on these SNPs and to uncover the associations with reproductive traits in ILD.Materials and Methods:A total of 280 ILDs consisting of Tegal and Magelang (F0) ducks and their reciprocal crosses, namely, Gallang (F1) and Maggal (F1), were maintained and specific variables were recorded, that is, age at first egg, body weight at first egg, first egg weight, and EP, for 90 days. Allele and genotype frequencies were used to determine the Hardy-Weinberg (H-W) equilibrium. The association between the SNP genotypes of PRL and reproductive traits was analyzed using one-way analysis of variance, following the GLM procedure of SAS. The genotypic effects on the reproductive traits were determined using regression analysis.Results:This study successfully amplified a polymerase chain reaction product of 190 bp, which was used to identify the SNP. Results indicated that PRL in ILDs is polymorphic. A SNP was found at position 164 nt (c.164G >A), consisting of three different genotypes, namely, GG, GA, and AA. The genotypes of Tegal and Magelang (F0), and Gallang (F1) populations were not in H-W equilibrium. The Maggal population (F1) was in H-W equilibrium. Significant associations were detected between the genotypes and EP in all ILDs (p<0.01), following a regression line of y=2.337x+64.605, with a determination coefficient of 0.0188 (r=0.14).Conclusion:PRL can be recommended as a candidate gene for reproductive traits in ILD, especially EP.

Exon and Intron Variability in Salmonidae Growth Hormone Genes

Gene duplication is one of the main mechanisms of formation of new genetic material in evolution. The occurrence of a gene duplication is believed to relax selection pressure on one of the copies. Consequently, this gene accumulates mutations at a higher rate, and over time it acquires a new function. As a result of several rounds of polyploidization, many genes in salmon are duplicated, including the growth hormone gene. The analysis of nucleotide diversity in the paralogous genes of growth hormone, gh1 and gh2, demonstrated that the level of variability in their introns was higher than in the exons. In addition, the variability of each exon weakly correlated with its length, and seems to be determined by the functional significance of the protein region encoded. The level of variability in the exons of the gh2 gene was higher than that in the gh1 one, which was probably due to the current process of gene subfunctionalization.

Межпородный анализ генетической структуры по генам гормона роста и инсулиноподобного фактора роста-1 крупного рогатого скота, разводимого на территории Республики Казахстан

Межпородный анализ генетической структуры по генам гормона роста и инсулиноподобного фактора роста-1 крупного рогатого скота, разводимого на территории Республики Казахстан

434 Late-Breaking: Expression of Growth hormone Gene (GH) and Insulin-like growth factor (IGF-2) in metabolic tissues of pigs are affected by unconventional dietary feed sources

Abstract This study was conducted to examine the growth performance and expression patterns of growth hormone gene and insulin-like growth factor in metabolic tissues of pigs fed different unconventional feed sources. A total of 45 growing pigs were randomly assigned to 5 dietary treatments at 9 animals per treatment and 3 animals per replicate. Treatment one (T1) comprise of whole maize (WM) which served as the control, T2: brewers dried grain (BDG), T3: cassava peel meal (CPM), T4: plantain peel meal (PPM) and T5: corn husk meal (CHM) at 35% inclusion in the diet. Data collected and analyzed includes; initial body weight (IBW), final body weight (FBW), average daily feed intake (ADFI), average daily weight gain (ADG), feed conversion ratio (FCR) and feed efficiency (FE). At the end of the feeding trial, three animals per treatment (one animal per replicate) were slaughtered; the duodenum and liver were collected and stored using RNALater prior to RNA extraction. Real-time qPCR was performed in 40cycles using the PowerUp SYBR Green reagent and analysed using the Delta-Delta Ct method. All data were subjected to analysis of variance in a completely randomized design. The results showed that the treatment diets had effect on growth performance with significant differences (P &amp;lt; 0.05) observed for average final weight, average total weight gain, average daily feed intake, feed conversion ratio and feed efficiency while the initial weight were not significantly different (P &amp;gt; 0.05). IGF-2 was differentially expressed in the liver and duodenum across treatments while significant differences were not observed in the expression pattern of the growth hormone gene in both tissues. This study showed promise for the use of unconventional feeding stuffs in pig feeding and reveals that genes influencing performance characters are differentially expressed in relation to the source of unconventional feeds used in compounding pig diet.

Knocking out histone methyltransferase PRMT1 leads to stalled tadpole development and lethality in Xenopus tropicalis

BackgroundAsymmetric arginine dimethylation of histone H4R3 to H4R3me2a by protein arginine methyltransferase 1 (PRMT1) has been implicated to play a key role in gene activation throughout vertebrates. PRMT1 knockout in mouse leads to embryonic lethality. This and the uterus-enclosed nature of the mouse embryo make it difficult to determine the development role of PRMT1 in mammals. MethodsWe took advantage of the external development of the diploid anuran Xenopus tropicalis and adapted the TALEN genome editing technology to knock out PRMT1 in order to investigate how PRMT1 participates in vertebrate development. ResultsWe observed that PRMT1 knockout had no apparent effect on embryogenesis because normally feeding tadpoles were formed, despite the reduced asymmetric H4R3 di-methylation (H4R3me2a) due to the knockout. However, PRMT1 knockout tadpoles had severely reduced growth even with normal growth hormone gene expression. These tadpoles were also stalled in development shortly after feeding began at stages 44/45 and died within 2 weeks, well before the onset of metamorphosis. In situ analyses revealed broad cessation or drastic reduction in cell proliferation in diverse organs including the eye, brain, spinal cord, liver, and intestine. ConclusionsOur findings suggest that PRMT1 is not required for embryogenesis but is a key regulator for normal progression of vertebrate development and growth. General significanceThe similarities and differences between PRMT1 knockout Xenopus tropicalis and mouse suggest that two distinct phases of vertebrate development: early embryogenesis and subsequent growth/organ maturation, have different but evolutionally conserved requirement for epigenetic modifications.

Identification of GH Gene Polymorphisms and Their Association with Body Weight in Bayang Duck, Local Duck from West Sumatra, Indonesia

Polymorphisms in the growth hormone (GH) gene that is associated with the growth rate of duck have been the target of many breeding programmes. Molecular market selection has been an acceptable tool in the acceleration of the genetic response of desired to improve production performance in livestock. Growth hormone (GH) has been considered as a candidate gene for growth traits. In this study, polymorphisms of the GH gene were evaluated for associations with body weight in 210 Bayang duck. The objective of this study was to assess the association of polymorphisms in Growth Hormone (GH) genes with growth in Bayang ducks. The polymorphism of the ducks GH gene from promoter region into exon 3 was researched by polymerase chain reaction and DNA sequencing methods. Fifty duck were genotyped by sequencing twenty mutations were detected in the Bayang duck GH gene. Mutation G→A were detected at position -142, 1155, 1117, 1423, and 1786. Mutation C→T were detected at position -62, 506, and 308. Mutation A→G were detected at position 160, 264, 293, 1245, and 2542. Mutation T→G detected at position 250 and mutation TT→AA detected at position 350. Mutation G→T detected at position 775 and mutation T→C were detected at position 1353, 1424, and 2973. All polymorphism were polymorphics except polymorphism T→G at position 250 was monomorphics. Mutation G→A in position 1117 and 1786 were restriction with enzyme Eco721 and TscaI. In the case GH-Eco721 three genotypes were observed, GG, GA, and AA with frequency 0.041, 0.59 and 0.369 respectively. For GH-TscaI locus the frequency were 0.034, 0.782, and 0.184 respectively. The effect of GH-TscaI polymorphism were observed on body weight in 4 to 8 week of Bayang duck.

Investigation of GH and GHRAlu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR. PCR products were digested by Alu I (GH, n: 167) and Alu I (GHR, n: 192) restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL (78.44%) and LV (21.56%) genotypes and L (0.89) and V (0.11) alleles for the GH gene and the AA (7.81%) and AG (92.19%) genotypes and A (0.54) and G (0.46) alleles for the GHR gene were obtained. Gene polymorphisms were not detected (P > 0.05) on the GH gene, but a significant difference was found for the GHR gene (P < 0.001). Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.

ХАРАКТЕРИСТИКА МОЛОЧНОЙ ПРОДУКТИВНОСТИ КОРОВ-ПЕРВОТЕЛОК С РАЗНЫМИ ГЕНОТИПАМИ СОМАТОТРОПИНА (GH)

. Polymorphisms of genes may be useful as genetic markers for additional selection criteria in dairy cattle breeding for economically advantageous traits. The presented study is dedicated to the influence of gene polymorphism, responsible for milk quality and milk productivity was carried out among Holstein cow-heifers in Integrated Agricultural Production Centre “Stud farm named after Lenin” of Atninsky district of the Republic of Tatarstan. DNA samples were isolated from blood samples of 130 cows for genotyping according to gene of growth hormone (GH) by PCR-RFLP analysis method. The genotypes distribution of the somatotropin gene were as follows: LL – 63.8% (83 animals), LV – 30.0% (39 animals), VV – 6.2% (8 animals), the distribution in frequency of occurrence of alleles L - 0.788 and V - 0.212. When considering the gene polymorphism association with the signs of milk production and the qualitative composition of milk showed prominent results cow-heifers with a LL genotype. The identified relationships indicate the possible genetic improvement of the investigated herds using marker-assisted selection.

Mutations in growth hormone gene affect stability of protein structure leading to reduced growth, reproduction, and milk production in crossbred cattle—an insight

The GH has a multifaceted role in growth, reproduction, and milk production. Nonsynonymous mutations identified as L153V were observed from GH1 (L) to GH2 (V) with higher genotypic frequency for GH1 being 0.87. GH2 (V) allele was identified as a rare allele and the population followed Hardy–Weinberg equilibrium. LL homozygote variant had significantly better growth, reproduction, and expected milk production at different ages in crossbred (CB) males. Reports are scanty explaining the molecular mechanism of how individuals with LV genotype were phenotypically inferior to that of wild-type LL. In the present study, it was explored that GH peptide with LV heterozygotes of GH gene, were observed to have reduced structural stability thermodynamically and thus functionally leads to reduced economic traits in CB animals. The fact was first time reported and confirmed through genomic analysis, bioinformatics, and later confirmed through immunohistochemistry. Differential expression analysis of the GH gene with respect to other genes in the hypothalamus-pituitary growth axis of CB cattle was also studied to have a complete insight of the GH gene.

Variation Amount of Sperm Cells in the First Generation of Nontransgenic and Transgenic Mutiara Catfish Hybrid

One of the factors that influence the success of catfish spawning, including male broodstock gonad fertility determine differences in the number of the sperm produced. The use of transgenesis technology (insertion of the exogenous growth hormone gene) in catfish shows an influence especially multiplication of the sperm counts. The purpose of this study was to compare the variations in the number of sperm cells of the male transgenic Mutiara catfish (comprising the African catfish growth hormone gene) with nontransgenic Mutiara. These research was conducted at the Fish Hatchery, Fisheries Biotechnology Laboratory, and Central Laboratory of Padjadjaran University. The experimental method was used completely randomized design and analyzed quantitatively and qualitatively. The male catfish broodstock were used as treatments consists of the first generation transgenic Mutiara hybrids with nontransgenic Mutiara (G1_MT-MNT), transgenic Mutiara hybrids with Sangkuriang (G1_MTS), and Sangkuriang (G1_S). The sperm from testes of each broodstock were used as replications for sperm count test parameters and motility (n = 5). Qualitative analysis was used for the identification parameters of the transgenic male broodstock using the Polymerase Chain Reaction (PCR) and sperm volume. Quantitative analysis uses the Sigma Plot 12.0 program for parameters for sperm cell count and sperm motility. The results showed that the highest number of sperm (6.06 x 106 cells) and the highest motility (score 4.2) were found in the broodstock of the G1_MT-MNT male catfish.

Identification of Bovine Growth Hormone (BGH) Gene Polymorphism Using PCR-RFLP Method in Buffalo Bulls

Growth Hormone (GH) is a single polypeptide chain synthesised and secreted from anterior pituitary gland by somatroph cells. The product of GH gene hastens metabolism and promotes the growth of many organs and tissues especially bone, muscle and visceral organs. It also regulates growth, mammary gland development and lactation. Polymorphism in this gene is associated with increase in growth and development of many tissues in the body.&#x0D; Aim: The objective of this study was to investigate the polymorphism of bovine growth hormone (bGH) gene in buffalo bulls (Bubalus bubalis) using the PCR-RFLP (polymerase chain reaction–restriction fragment length polymorphism) technique.&#x0D; Design: Genomic DNA was extracted from a total of 10 bulls, consisting of Murrah – Swamp crossbred and pure Swamp buffalo bulls. A The 446 segment of the bGH gene was amplified. The DNA amplicons were detected in 2% agarose gel following 45 minutes of electrophoresis. They were thereafter digesting with AluI endonuclease restriction enzyme, and the digested DNA were detected in 2% agarose gel following electrophoresis for about 45minutes in all samples&#x0D; Results: Similar bands of approximately 300 and 146-bp each, with no variation, were detected in 2% agarose gel following electrophoresis in all the animals tested.&#x0D; Conclusion: Based on the Alu1 digestion result, all samples produced the same allele of the gene, with no polymorphism detected.