Gregarious Locusts Research Articles

Gregarious desert locusts have substantially larger brains with altered proportions compared with the solitarious phase

The behavioural demands of group living and foraging have been implicated in both evolutionary and plastic changes in brain size. Desert locusts show extreme phenotypic plasticity, allowing brain morphology to be related to very different lifestyles in one species. At low population densities, locusts occur in a solitarious phase that avoids other locusts and is cryptic in appearance and behaviour. Crowding triggers the transformation into the highly active gregarious phase, which aggregates into dense migratory swarms. We found that the brains of gregarious locusts have very different proportions and are also 30 per cent larger overall than in solitarious locusts. To address whether brain proportions change with size through nonlinear scaling (allometry), we conducted the first comprehensive major axis regression analysis of scaling relations in an insect brain. This revealed that phase differences in brain proportions arise from a combination of allometric effects and deviations from the allometric expectation (grade shifts). In consequence, gregarious locusts had a larger midbrainoptic lobe ratio, a larger central complex and a 50 per cent larger ratio of the olfactory primary calyx to the first olfactory neuropile. Solitarious locusts invest more in low-level sensory processing, having disproportionally larger primary visual and olfactory neuropiles, possibly to gain sensitivity. The larger brains of gregarious locusts prioritize higher integration, which may support the behavioural demands of generalist foraging and living in dense and highly mobile swarms dominated by intense intraspecific competition.

Molecular cloning and temporal–spatial expression of I element in gregarious and solitary locusts

It has been reported that many genes and small RNAs are associated with density-dependent polyphenism in locusts. However, the regulatory mechanism underlying gene transcription is still unknown. Here, by analysis of transcriptome database of the migratory locust, we identified abundant transcripts of transposable elements, which are mediators of genetic variation and gene transcriptional regulation, mainly including CR1, I, L2 and RTE-BovB. We cloned one I element, which represents the most abundant transcripts in all transposable elements, and investigated its developmental and tissue-specific expression in gregarious and solitary locusts. Although there are no significant differences of I element expression in whole bodies between gregarious and solitary locusts at various developmental stages, this I element exhibits high expression level and differential expression pattern between gregarious and solitary locusts in central and peripheral nervous tissues, such as brain, antenna and labial palps. These results suggest that I element is potentially involved in the response of neural systems to social environmental changes in locusts.

Motor neurone responses during a postural reflex in solitarious and gregarious desert locusts

Desert locusts show extreme phenotypic plasticity and can change reversibly between two phases that differ radically in morphology, physiology and behaviour. Solitarious locusts are cryptic in appearance and behaviour, walking slowly with the body held close to the ground. Gregarious locusts are conspicuous in appearance and much more active, walking rapidly with the body held well above the ground. During walking, the excursion of the femoro-tibial (F-T) joint of the hind leg is smaller in solitarious locusts, and the joint is kept more flexed throughout an entire step. Under open loop conditions, the slow extensor tibiae (SETi) motor neurone of solitarious locusts shows strong tonic activity that increases at more extended F-T angles. SETi of gregarious locusts by contrast showed little tonic activity. Simulated flexion of the F-T joint elicits resistance reflexes in SETi in both phases, but regardless of the initial and final position of the leg, the spiking rate of SETi during these reflexes was twice as great in solitarious compared to gregarious locusts. This increased sensory-motor gain in the neuronal networks controlling postural reflexes in solitarious locusts may be linked to the occurrence of pronounced behavioural catalepsy in this phase similar to other cryptic insects such as stick insects.

The cloning, phylogenetic relationship and distribution pattern of two new putative GPCR-type octopamine receptors in the desert locust ( Schistocerca gregaria)

The biogenic amine octopamine functions as a neuromodulator, neurotransmitter and neurohormone in insect nervous systems. It plays a prominent role in modulating multiple physiological and behavioural processes in invertebrates. Octopamine exerts its effects by binding to specific receptor proteins that belong to the superfamily of G protein-coupled receptors. We found two partial sequences of putative octopamine receptors in the desert locust Schistocerca gregaria (SgOctαR and SgOctβR) and investigated their transcript levels in males and females of both phases and during the transition between long-term solitarious and gregarious locusts. The transcript levels of SgOctαR are the highest in the central nervous system, whereas those of SgOctβR are the highest in the flight muscles, followed by the central nervous system. Both SgOctαR and SgOctβR show higher transcript levels in long-term gregarious locusts as compared to solitarious ones. The rise of SgOctβR transcript levels already appears during the first 4 h of gregarisation, during which also the behavioural changes take place.

Effect of Paranosema (Nosema) locustae (Microsporidia) on morphological phase transformation of Locusta migratoria manilensis (Orthoptera: Acrididae)

Field and laboratory studies demonstrated that Paranosema (Nosema) locustae had significant effects on the morphological phase transformation of Locusta migratoria manilensis (Meyen 1835). In the field, spraying P. locustae on gregarious locusts caused a substantial population reduction by 16 days after treatment, with most of the surviving locusts being phase solitaria. However, the effects of P. locustae on locust phase transformation began before direct mortality had caused a substantial reduction in locust density: locust numbers were still high at day 10, but locusts had already transformed to phase transiens. Laboratory assays showed that while a low dose of P. locustae had no effect on phase transformation, at a higher dose of 1×105 spores/mL, locusts had F/C ratios that were significantly (P<0.05) more solitaria than untreated locusts, with locusts having ratios that were either phase solitaria or on the solitaria side of phase transiens. In a second laboratory experiment that analysed the effects of locust density on phase transformation by P. locustae, there was no obvious effect of density on female locusts 10 days later as all were solitaria at all locust densities. At day 16, female locusts were transiens at higher densities, but were solitaria at 4/cage. With males there were lesser effects. These results provide new evidence for P. locustae having sub-lethal effects on locust phase transformation at a wide range of locust densities.

The role of octopamine in insects and the cloning, phylogenetic relationship and distribution pattern of three new putative octopamine GPCR’s in the desert locust

Event Abstract Back to Event The role of octopamine in insects and the cloning, phylogenetic relationship and distribution pattern of three new putative octopamine GPCR’s in the desert locust Heleen Verlinden1*, Elisabeth Marchal1, Wolfgang Blenau2, Hans-Joachim Pflüger3 and Jozef V. Broeck1 1 Catholic University of Leuven, Molecular Developmantal Physiology and Signal Transduction, Animal Physiology and Neurobiology, Zoological Institute, Belgium 2 Universität Potsdam, Institute of Biochemistry and Biology, Animal Physiology, Germany 3 Freie Universität Berlin, Institute of biology, Neurobiology, Germany The biogenic amine octopamine functions as a neuromodulator, neurotransmitter and neurohormone in insect nervous systems. It plays a prominent role in modulating multiple physiological and behavioural processes in insects. Octopamine exerts its effects by binding to specific proteins that belong to the superfamily of GPCR's. We picked up three partial sequences of putative octopamine receptors in S. gregaria (SgOctαR1, SgOctαR2 and SgOctβR) using degenerated primers and investigated their transcript levels in males and females of both phases and during the transition between long-term solitarious and gregarious locusts. The transcript levels of the SgOctαR's are the highest in the CNS, whereas those of SgOctβR are the highest in the flight muscles, followed by the CNS. Both SgOctαR1 and SgOctβR show higher transcript levels in long-term gregarious locusts compared to solitarious ones. The rise of SgOctβR transcript levels already appears during the first four hours of gregarisation, in which also the behavioural changes take place. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Verlinden H, Marchal E, Blenau W, Pflüger H and Broeck JV (2010). The role of octopamine in insects and the cloning, phylogenetic relationship and distribution pattern of three new putative octopamine GPCR’s in the desert locust. Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00055 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Heleen Verlinden, Catholic University of Leuven, Molecular Developmantal Physiology and Signal Transduction, Animal Physiology and Neurobiology, Zoological Institute, Leuven, Belgium, heleen.verlinden@bio.kuleuven.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Heleen Verlinden Elisabeth Marchal Wolfgang Blenau Hans-Joachim Pflüger Jozef V Broeck Google Heleen Verlinden Elisabeth Marchal Wolfgang Blenau Hans-Joachim Pflüger Jozef V Broeck Google Scholar Heleen Verlinden Elisabeth Marchal Wolfgang Blenau Hans-Joachim Pflüger Jozef V Broeck PubMed Heleen Verlinden Elisabeth Marchal Wolfgang Blenau Hans-Joachim Pflüger Jozef V Broeck Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Spatiotemporal receptive field properties of a looming-sensitive neuron in solitarious and gregarious phases of the desert locust.

Desert locusts (Schistocerca gregaria) can transform reversibly between the swarming gregarious phase and a solitarious phase, which avoids other locusts. This transformation entails dramatic changes in morphology, physiology, and behavior. We have used the lobula giant movement detector (LGMD) and its postsynaptic target, the descending contralateral movement detector (DCMD), which are visual interneurons that detect looming objects, to analyze how differences in the visual ecology of the two phases are served by altered neuronal function. Solitarious locusts had larger eyes and a greater degree of binocular overlap than those of gregarious locusts. The receptive field to looming stimuli had a large central region of nearly equal response spanning 120 degrees x 60 degrees in both phases. The DCMDs of gregarious locusts responded more strongly than solitarious locusts and had a small caudolateral focus of even further sensitivity. More peripherally, the response was reduced in both phases, particularly ventrally, with gregarious locusts showing greater proportional decrease. Gregarious locusts showed less habituation to repeated looming stimuli along the eye equator than did solitarious locusts. By contrast, in other parts of the receptive field the degree of habituation was similar in both phases. The receptive field organization to looming stimuli contrasts strongly with the receptive field organization of the same neurons to nonlooming local-motion stimuli, which show much more pronounced regional variation. The DCMDs of both gregarious and solitarious locusts are able to detect approaching objects from across a wide expanse of visual space, but phase-specific changes in the spatiotemporal receptive field are linked to lifestyle changes.

The role of egg pod foam and rearing conditions of the phase state of the Asian migratory locust Locusta migratoria migratoria ( Orthoptera, Acrididae)

Coloration phase state, morphometrical ratios and the numbers of mature oocytes of Locusta migratoria migratoria were examined in a series of experiments to determine the means by which phase characteristics are passed to the next generation. Washing with distilled water of eggs from egg pods laid by gregarious crowd-reared females resulted in solitarization of the hatchlings after their isolation, indicating that a factor present in eggs encapsulated in foam is causal to gregarization. Such locusts showed a significant shift towards the typical solitarious body coloration, morphometry and number of mature oocytes as compared to locusts resulting from unwashed eggs. Gregarious coloration, morphometrical ratios and oocyte numbers could be partially restored when hatchlings from washed eggs were regrouped. When gregarious locusts were reared in isolation, they showed a solitary body color, whereas, morphometry and oocyte numbers were not affected by isolation.

Serotonin Mediates Behavioral Gregarization Underlying Swarm Formation in Desert Locusts

Desert locusts, Schistocerca gregaria, show extreme phenotypic plasticity, transforming between a little-seen solitarious phase and the notorious swarming gregarious phase depending on population density. An essential tipping point in the process of swarm formation is the initial switch from strong mutual aversion in solitarious locusts to coherent group formation and greater activity in gregarious locusts. We show here that serotonin, an evolutionarily conserved mediator of neuronal plasticity, is responsible for this behavioral transformation, being both necessary if behavioral gregarization is to occur and sufficient to induce it. Our data demonstrate a neurochemical mechanism linking interactions between individuals to large-scale changes in population structure and the onset of mass migration.

Endocrinology of reproduction and phase transition in locusts

In the last decade, important progress has been made in the experimental analysis of the endocrine mechanisms controlling reproduction and phase transition in locusts. Phase transition is a very fascinating, but complex, phenomenon of phenotypic plasticity that is triggered by changes in population density and can lead to the formation of extremely devastating hopper bands and adult gregarious locust swarms. While some phase characteristics change within hours, others appear more gradually in the next stage(s), or even in the next generation(s). In adults, the phase status also has a major influence on the process of reproduction. A better understanding of how solitarious locusts become gregarious and how this switch affects reproductive physiology may result in novel strategies to fight locust plagues. In this paper, we will review the current knowledge concerning this close interaction between locust phase polyphenism and reproduction.

Phylogeny of bird-grasshopper subfamily Cyrtacanthacridinae (Orthoptera: Acrididae) and the evolution of locust phase polyphenism.

Locust phase polyphenism is an extreme form of density-dependent phenotypic plasticity in which solitary and cryptic grasshoppers can transform into gregarious and conspicuous locusts in response to an increase in local population density. We investigated the evolution of this complex phenotypic plasticity in a phylogenetic framework using a morphological phylogeny of Cyrtacanthacridinae, which contains some of the most important locust species, and a comprehensive literature review on the biology and ecology of all known members of the subfamily. A phylogenetic analysis based on 71 morphological characters yielded a well-resolved tree and found that locust phase polyphenism evolved multiple times within the subfamily. The literature review demonstrated that many cyrtacanthacridine species, both locust and sedentary, are capable of expressing density-dependent color plasticity. When this color plasticity was divided into two smaller components, background coloration and development of black pigmentation, and when these plastic traits were optimized on to the phylogeny, we found that the physiological mechanisms underlying this plasticity were plesiomorphic for the subfamily. We also found that different locust species in Cyrtacanthacridinae express both similarities and differences in their locust phase polyphenism. Because locust phase polyphenism is a complex syndrome consisting of numerous plastic traits, we treat it as a composite character and dissected it into smaller components. The similarities among locust species could be attributed to shared ancestry and the differences could be attributed to the certain components of locust phase polyphenism evolving at different rates. © The Willi Hennig Society 2007.

Arousal Facilitates Collision Avoidance Mediated by a Looming Sensitive Visual Neuron in a Flying Locust

Locusts have two large collision-detecting neurons, the descending contralateral movement detectors (DCMDs) that signal object approach and trigger evasive glides during flight. We sought to investigate whether vision for action, when the locust is in an aroused state rather than a passive viewer, significantly alters visual processing in this collision-detecting pathway. To do this we used two different approaches to determine how the arousal state of a locust affects the prolonged periods of high-frequency spikes typical of the DCMD response to approaching objects that trigger evasive glides. First, we manipulated arousal state in the locust by applying a brief mechanical stimulation to the hind leg; this type of change of state occurs when gregarious locusts accumulate in high-density swarms. Second, we examined DCMD responses during flight because flight produces a heightened physiological state of arousal in locusts. When arousal was induced by either method we found that the DCMD response recovered from a previously habituated state; that it followed object motion throughout approach; and--most important--that it was significantly more likely to generate the maintained spike frequencies capable of evoking gliding dives even with extremely short intervals (1.8 s) between approaches. Overall, tethered flying locusts responded to 41% of simulated approaching objects (sets of 6 with 1.8 s ISI). When we injected epinastine, the neuronal octopamine receptor antagonist, into the hemolymph responsiveness declined to 12%, suggesting that octopamine plays a significant role in maintaining responsiveness of the DCMD and the locust to visual stimuli during flight.

Quantitative RT‐PCR analysis of pacifastin‐related precursor transcripts during the reproductive cycle of solitarious and gregarious desert locusts

In locusts, little is known about the physiological and biochemical mechanisms regulating complex processes, such as reproduction and phase transition. The pacifastin family constitutes a family of peptidic inhibitors of serine proteases that are considered to be important regulators of several physiological processes in arthropods. We have performed a detailed transcript profiling analysis of two pacifastin-related peptide precursors, SGPP-2 and SGPP-4, during the reproductive cycle of adult desert locusts (Schistocerca gregaria). This quantitative real-time (RT)-PCR analysis revealed a temporal regulation of both transcripts, which is paralleled by several events that occur during the reproductive cycle of adult locusts. The observed temporal transcript profiles display a strong tissue-, gender- and phase-dependence. In addition, a partial regregarization experiment suggests that both transcript levels are regulated during phase transition and can be employed as molecular markers of the gregarization process.

Swarm formation in the desert locust Schistocerca gregaria:isolation and NMR analysis of the primary maternal gregarizing agent

Depending on their rearing density, female desert locusts Schistocerca gregaria epigenetically endow their offspring with differing phenotypes. To identify the chemical basis for such maternal transmission, we compared solitarious and gregarious locust egg pod foam using high performance liquid chromatography (HPLC). We revealed causal relationships between foam chemistry and hatchling phenotype (phase state) by iteratively applying HPLC fractions from gregarious locust egg foam extracts to solitarious eggs and assessing resulting hatchlings with a behavioural bioassay involving logistic regression. Selection and application of increasingly specific HPLC fractions allowed us to isolate compounds with gregarizing properties. Hatchling gregarization was triggered only by certain fractions and was dose dependent. In a final series of experiments, we characterized the most specific gregarizing fraction by nuclear magnetic resonance (NMR) spectroscopy. Here we present tentative structural features of the primary locust maternal gregarizing agent, which appears to be an alkylated l-dopa analogue. In addition, we propose a mechanism for phase-dependent regulation of this compound's activity.

Nutritional ecology of Schistocerca piceifrons piceifrons adults from Socorro Island, Mexico

Nutritional ecology of the Central American locust Schistocerca piceifrons piceifrons was studied using specimens from Socorro Island, Mexico. They were fed in the laboratory with common plants obtained from the island: Ficus cotinifolia (Moraceae), Cordia curassavica (Boraginaceae), Nicotiana stocktonii (Solanaceae), and Psidium sartorianum (Myrtaceae). We measured nutritional and growth rates in dry weights: intake, gain in weight, as well as some Waldbauer indices equivalents: efficiency of conversion of ingested food (ECI), efficiency of conversion of digested (absorbed) food ( ECD), and approximate digestibility (AD). We compared nutritional indices equivalents between female and male, between gregarious and solitary phases and between diets, employing ANCOVAs. We found no differences in metabolic efficiencies between female and male locusts, no differences between gregarious and solitary locusts. We found that F. cotinifolia is a food plant that favors the intake, nutritional efficiencies and steady growth of the Central American locust, in comparison to other food plants.

Compensatory Plasticity at an Identified Synapse Tunes a Visuomotor Pathway

We characterized homeostatic plasticity at an identified sensory-motor synapse in an insect, which maintains constant levels of motor drive as locusts transform from their solitarious phase to their gregarious swarming phase. The same mechanism produces behaviorally relevant changes in response timing that can be understood in the context of an animal's altered behavioral state. For individual animals of either phase, different looming objects elicited different spiking responses in a visual looming detector interneuron, descending contralateral movement detector (DCMD), yet its synaptic drive to a leg motoneuron, fast extensor tibiae (FETi), always had the same maximum amplitude. Gregarious locust DCMDs produced more action potentials and had higher firing frequencies, but individual postsynaptic potentials (PSPs) elicited in FETi were half the amplitude of those in solitarious locusts. A model suggested that this alone could not explain the similarity in overall amplitude, and we show that facilitation increased the maximum compound PSP amplitude in gregarious animals. There was the same linear relationship between times of peak DCMD firing before collision and the size/velocity of looming objects in both phases. The DCMD-FETi synapse transformed this relationship nonlinearly, such that peak amplitudes of compound PSPs occurred disproportionately earlier for smaller/faster objects. Furthermore, the peak PSP amplitude occurred earlier in gregarious than in solitarious locusts, indicating a differential tuning. Homeostatic modulation of the amplitude, together with a nonlinear synaptic transformation of timing, acted together to tune the DCMD-FETi system so that swarming gregarious locusts respond earlier to small moving objects, such as conspecifics, than solitarious locusts.

CDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust

The high population density of insects is often a stress factor. Insects synthesize heat shock proteins (Hsps) in response to the impacts of stress through molecular chaperone activity. Locust solitary and gregarious phases occur at low and high population density, respectively. In this study, we compare the expression profiles of the Hsp genes in the two phases of the migratory locust in an attempt to examine the role of Hsps in adaptation to high density populations. The full length cDNAs of Hsp20.5, 20.6, 20.7, 40, 70 and Hsp90 of the migratory locust were cloned and sequenced. The expressional differentiation of the six Hsps in mRNA levels between solitary and gregarious locusts was observed. Results from real-time PCR indicate that the six Hsps are expressed throughout all developmental stages except in the early stage embryo. The expression levels of the six Hsps were significantly upregulated in gregarious locusts. The expressional variations among certain organs, such as the head, thorax and leg of fifth instar nymphs in gregarious locusts were also higher than those in solitary ones. These observations suggest that population density may be an important factor in determining Hsp expression in the locust.

A miniaturized assay to quantify effects of chemicals or physical stimuli upon locust activity

Abstract Solitary and gregarious locusts differ in many traits, such as body color, morphometrics and behavior. With respect to behavior, solitary animals shun each other, while gregarious animals seek each other's company, hence their crowding behavior. General activity, depending on the temperature, occurs throughout the day but is much lower in solitary locusts. Solitary locusts occasionally fly by night while gregarious locusts fly regularly during daytime (swarming). In search of new assays to identify substances that control or modify aspects of (phase) behavior, we designed a simple activity assay, meant to complement existing behavioral measurement tools. The general activity is reflected in the number of wall hits, that is, the number of contacts between the locust and the vertical walls of a small arena. Using this single parameter we were able to quantify differences in total activity of both nymphs and adults of isolation‐reared (solitary), regrouped‐ and crowd‐reared (gregarious) locusts under different conditions. Furthermore, we demonstrate that there are inter‐ and intra‐phase dependent differences in activities of 5th instar nymphs after injections of the three different adipokinetic hormones.

Regulation of Schistocerca gregaria neuroparsin transcript levels by juvenile hormone and 20‐hydroxyecdysone

Neuroparsins (NPs) are small proteins that were originally discovered in the pars intercerebralis-corpus cardiacum neurosecretory complex of the migratory locust brain. From the desert locust, Schistocerca gregaria, we recently cloned four different transcripts, each coding for a distinct NP-related peptide. In addition to the brain, some NP-like precursor (Scg-NPP) transcripts also occur in a number of peripheral tissues, and their expression levels are controlled in a gender- and stage-dependent manner. Previous studies revealed a close correlation between Scg-NPP transcript levels and the gonotrophic cycle. In the present report, we demonstrate that certain Scg-NPP transcript levels are significantly altered upon injection of juvenile hormone (JH) or 20-hydroxyecdysone (20E) in adult gregarious desert locusts (five days after final ecdysis). While Scg-NPP1 transcript levels did not significantly change as a result of hormone treatment (animals were analyzed 24 h after injection), Scg-NPP2, Scg-NPP3, and Scg-NPP4 displayed hormone-dependent regulation in various tissues. Scg-NPP2 and Scg-NPP3 transcript levels significantly increased in the brain of JH-treated locusts. In addition, JH induction of Scg-NPP3 and Scg-NPP4 transcripts was observed in male fat body and in male and female gonads. Furthermore, 20E injection also induced Scg-NPP2, Scg-NPP3, and Scg-NPP4 transcripts in desert locust gonads. This is the first report showing NP-like precursor gene expression in insect ovaries. Our study indicates that the expression levels of some Scg-NPP transcripts are regulated by developmental hormones, suggesting a close correlation between NP expression and the endocrine control of the reproductive cycle.

Neuroparsin transcripts as molecular markers in the process of desert locust ( Schistocerca gregaria) phase transition

Desert locust swarms occasionally cause severe ecological and economic damage, particularly in countries of northwest Africa. However, the physiological mechanisms underlying locust phase transition, the switch of the solitarious to the gregarious phase, remain elusive. Therefore, identification of molecular changes linked to this phenomenon represents a primary requirement to start unraveling this enigma. The present paper provides novel information on phase-related molecular markers for locust phase transition. We present a detailed quantitative real-time RT-PCR analysis of two distinct neuroparsin precursor transcripts ( Scg-NPP3 and Scg-NPP4) in the brain and in abdominal tissues of gregarious and solitarious desert locusts ( Schistocerca gregaria). Our data reveal different temporal changes of these transcripts in the fat body during the adult stage of both phases. We, hereby, present novel scientific evidence for a phase-dependent regulation of these particular peptide hormone encoding transcripts and assign them as possible molecular markers in the process of locust phase transition.