Green Shell Research Articles

Assessing coconut shell pyrolysis: Biomass characterization, activation energy estimation, and statistical analysis of operating conditions

This study experimentally investigates the conventional pyrolysis of green coconut shell (GCS) in a fixed-bed reactor, analyzing operational conditions and their impact on product yields using statistical methods. Comprehensive characterization of GCS highlighted an extractive content of 36.65 %, significantly influencing the pyrolysis activation energy. Statistical analysis revealed that temperature has a more pronounced effect on yield over particle size. Optimal pyrolysis conditions were identified at 773.15 K with a particle diameter of 1.3 mm, achieving a maximum bio-oil yield of 49.45 %, surpassing the reported in previous fixed-bed reactor studies. Bio-oil analysis indicated aldehydes, phenols, and esters predominance, with temperature as the primary factor influencing their formation. The study demonstrates the effectiveness of GCS pyrolysis in fixed bed reactors for converting waste into valuable products with potential applications as energy sources, as well as in the chemical and pharmaceutical industries, thereby reducing environmental impacts associated with improper disposal.

Review on Seashell Ash as Partial Cement Replacement

The review paper focuses on utilizing various sea shell ashes, including cockle, clam, oyster, mollusc, periwinkle, snail, and green mussel shell ash, as partial replacements for cement. It aims to promote environmental sustainability and mitigate the issues associated with global warming. Cement production is known to have significant environmental impacts across all stages, including air pollution through dust and gases, as well as noise and vibration during quarrying and milling processes. One proposed solution is the adoption of modified cement, which refers to cementitious materials designed to match or surpass the performance of Portland cement while incorporating recycled and waste materials, thereby reducing the demand for raw materials and promoting sustainability in construction. Utilizing sea shell ash as a partial replacement for cement in concrete has the potential to yield substantial energy savings and offer significant environmental advantages. Previous investigations have explored the chemical and mechanical properties of concrete produced with sea shell ash replacements, including specific gravity, chemical composition, compressive strength, tensile strength, and flexural strength. The results indicate that the optimal replacement percentage for sea shell ash in cement lies within the range of 4–5%.

Adsorption of copper (II) from aqueous solutions using adsorbent obtained with sodium hydroxide activation of biochar prepared by microwave pyrolysis

AbstractMicrowave heating is one of the leading methods that save energy, time, and cost for the conversion of bio-waste into biochar in the agricultural sector. In this study, in the first stage, economical biochar production from pistachio green hulls by microwave heating method is in question. In the second step, sodium hydroxide(NaOH) activation was followed to improve the surface properties of biochar. This adsorbent obtained was applied to remove copper ions(Cu(II)) from wastewater. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), thermogravimetry differential thermal analysis (TG/DTG), and nitrogen adsorption/desorption methods were used to characterize the biochar samples. In adsorption studies, kinetic, isotherm, and thermodynamic parameters were investigated. Cu(II) adsorption capacity on modified biochar obtained by Langmuir isotherm was found to be 227.27 mg/g. In addition, the mechanism of Cu(II) adsorption on modified biochar has been proposed. Biochar, which is obtained from the green shells of pistachios, which is widely available and grown in Turkey, by microwave method and then NaOH activation, is a potential solution for both the improvement of water pollution caused by heavy metals and the disposal of related wastes with an appropriate method.

Hydrothermally synthesized-nanoscale carbonated hydroxyapatite with calcium carbonates derived from green mussel shell wastes

A hydrothermal method for exploring the benefits of a low-cost calcium carbonate precursor generated by mineral carbonation of green mussel wastes was in the study for synthesizing carbonated hydroxyapatite at various temperatures. Two typical precipitated calcium carbonates identified include vaterite and calcite particles formed by previous direct mineral carbonation. The hydrothermal assisted solution at an initial pH of 5–6, containing calcium carbonate precursor and diammonium hydrogen phosphate with a Ca/P ratio of 1.67, was autoclaved for carbonated hydroxyapatite (CHA) growth for 24 h at 50, 70, 90, and 110 °C and then allowed to air cool naturally. The hydrothermally obtained powder was subjected to additional analytical evaluation, including XRD, FTIR, and SEM/EDX analysis, providing that the crystal structure, carbonate content, and lattice constants of the A- and B-type CHA formed by varying hydrothermal temperature agreed very well with the A- and B-type carbonated hydroxyapatite as reported in the literature. The hydrothermal process could produce more than 90 wt.% of hydroxyapatite nanoparticles (17–26 nm) with minor monetite and calcite. These experimental findings may aid in synthesizing A- and B-type CHA for medical applications such as bone and enamel.

Transforming seafood waste: Green mussel shell-derived hydroxyapatite as a catalyst for spirooxindole synthesis

Utilizing bioresources and renewable materials represents a sustainable approach in chemical reactions. This study aims to harness seafood waste from green mussel shells as a raw material to produce hydroxyapatite (HAp) and employ it as a heterogeneous catalyst for synthesizing spirooxindole compounds. The green mussel shells underwent varying temperature calcination to yield CaO, subsequently utilized in HAp synthesis. XRD analysis exhibited the characteristic peak pattern of HAp and revealed a reduction in crystal sizes with increased calcination temperatures for CaO formation. Additionally, FTIR analysis identified the constituent functional groups of HAp in the sample. SEM analysis revealed irregularly shaped HAp particles, with average sizes of 268.42 nm (HAp-800), 212.46 nm (HAp-900), and 184.6 nm (HAp-1000). Using HAp as a catalyst in synthesizing spirooxindole yielded a 64 % product yield and demonstrated reusability. Utilizing mussel shell-derived hydroxyapatite as a catalyst offers a cost-effective and environmentally friendly approach to achieving higher product yields.

PcASTA in Procambarus clarkii, a novel astaxanthin gene affecting shell color

The color of crayfish (Procambarus clarkii) shells not only affects their value, but also has a significant impact on individual size. Astaxanthin is a common carotenoid that has been found in many studies to have different functions, including regulating body color formation. In this study, the levels of different carotenoids were detected using high performance liquid chromatography (HPLC) and found a significant correlation between astaxanthin content and shell color. An astaxanthin gene (PcASTA) was identified from P. clarkii, and it was expressed in tissues of crayfish with dark-red shells and green shells. The highest expression levels of PcASTA were found in the hepatopancreas, followed by the muscle. The expression of PcASTA in all tissues of red shell crayfish was higher than that of green shell crayfish, indicating that PcASTA may be involved in shell color formation in crayfish. Moreover, high water temperature and feeding β-carotene can lead to an increase in tissue expression of PcASTA and astaxanthin content in crayfish shells. The findings indicate that PcASTA may affect shell color in P. clarkii by being involved in astaxanthin synthesis and transformation.

Investigation of the antibacterial activity of synthesized hydroxyapatite Sr-doped nanocomposite

Hydroxyapatite is a mineral compound with a composition resembling the components found in human teeth and bones. Hydroxyapatite possesses osteoconductive, bioactive, and biocompatible characteristics. Nevertheless, hydroxyapatite has limitations, particularly its limited antibacterial activity. Therefore, this research uses the sol-gel method to synthesize and characterize strontium hydroxyapatite doped composites (HAp-Sr doped composites). The calcium precursor used in this research comes from the green mussel shells (Perna viridis); the main component is CaCO3, which will later be converted into CaO compounds. HAp Sr-doped composites were synthesized by varying the concentration of Sr metal (5%, 10%, and 20% w/w), which aimed to determine its good antibacterial ability. The X-ray fluorescence analysis reveals that green mussel shells contain 97.261% calcium oxide (CaO). The X-ray diffraction analysis reveals that the pattern observed at an angle of 2θ corresponds to the hydroxyapatite standard from ICDD (No.96-101-1243), which possesses a hexagonal structure. The Fourier transform infrared spectroscopy spectra reveal the presence of PO43− groups in the sample at wave numbers 528 cm−1, 604 cm−1, 945 cm−1, and 1,018 cm−1. The antibacterial activity of the HAp-Sr doped composite was evaluated against Staphylococcus aureus and Escherichia coli bacteria. The results indicated that the 5% HAp-Sr doped composite exhibited antibacterial activity. Therefore, it can potentially be utilized in bone grafts and implants.

Influence of Preheat Temperature Variation on Single Screw Extrusion for Characterizing PLA, PCL, and HA Biocomposite Filaments

Fractures and ligament injuries often require bone fixation, commonly through Open Reduction and Internal Fixation (ORIF) with compression plates and screws. Additive manufacturing (AM) techniques are considered sustainable due to their potential to reduce emissions and have a minimal adverse impact on the environment. Currently, there is extensive research into producing biocomposite filaments, though there remains limited exploration of the impact of preheat temperature on filament properties. This research explores the effects of varying preheat temperatures in a single screw extruder to achieve biocomposite filament as candidate for biodegradable bone implants. Biocomposite filaments made from Polylactic Acid (PLA) and Polycaprolactone (PCL) in an 85:15 ratio, with 5% hydroxyapatite (HA) derived from green mussel shell waste. Biocomposite mixture was cut into sub-5mm fragments and extruded at 175°C with preheat temperatures between 159°C and 168°C. Filaments were used to fabricate specimens via a 3D printer, following ASTM D790 standards. Specimens were analyzed using X-Ray Diffractometer, Fourier Transform Infrared, Scanning Electron Microscope, density measurements, three-point bending tests, and biodegradation assessments. Results show that preheat temperature variations significantly affect the filament’s crystallinity index, impacting density and mechanical properties. Higher preheat temperatures result in a lower crystallinity index, decreasing density and potentially increasing degradation rates.

Potential use of green coconut shell liquid in young dwarf coconut plants

Potential use of green coconut shell liquid in young dwarf coconut plants

Distribution Analysis of Green Mussels (Perna viridis) in Banyuurip Village, Ujung Pangkah District, Gresik Regency

The aim of the research was to determine the Distribution analysis of Perna viridis diversity based on the suitability map for green mussel cultivation, analysis of community perceptions and the condition of water bodies. Perna viridis aquaculture is suitable for the waters of Banyuurip village based on the variables of sea depth, sea surface temperature, chlorophyll a concentration, and substrate radiance data. Heavy metal content of Pb is located and distributed in polluted areas. It was found that the potential for green mussel aquaculture is greater in warm water habitats, i.e. closer to the coast. In addition, the northeast was identified as a suitable habitat for green mussels. The mid-ocean area in the north-west is of low suitability due to the lack of substrate and chlorophyll. Analysis of community attitudes and behaviour showed that people remain enthusiastic about green mussel farming, although green mussel shell waste remains an environmental problem. Heavy metal analysis of green mussel samples showed that green mussels are still safe for consumption. Therefore, the main conclusion is that the habitat of Perna viridis in the waters of Banyuurip Village still has potential for cultivation, but the waters must be free from pollution.

Characterization of β-tricalcium phosphate derived from green mussel shells (Molarity variation)

Characterization of β-tricalcium phosphate derived from green mussel shells (Molarity variation)

A combined experimental and DFT study of metal core/indocyanine green shell hybrid nanoparticles

Indocyanine green (ICG) is the FDA-approved fluorescent dye used for in vivo medical imaging, diagnostics, and photothermal therapy. However, this dye is easily degradable in the human vascular system, and therefore its stabilization is preferable. In this work, ICG molecules were stabilized by their adsorption on the surface of the L-methionine-capped Ag and Au nanoparticles (Ag and Au @LM NPs) in aqueous colloidal dispersions. The result is the formation of hybrid metal core/ICG shell NPs in colloidal dispersions. Additionally, colloidal dispersions were stabilized, indicating a double effect of ICG adsorption. The obtained hybrid NPs were studied experimentally (UV–Vis spectrophotometry, HRTEM, DLS, FTIR) and theoretically (DFT calculations). HRTEM revealed that the interplanar spacing between adjacent planes of NPs decreases after the dye adsorption. The results obtained from the DFT study confirmed the formation of a covalent bond between the oxygen from ICG dye SO3− group and metal NPs. Considering the characteristics of both components of the NPs/ICG hybrid system, the authors assume that this hybrid system can exhibit the synergistic effect that could lead to more successful theranostic treatment of cancer in nanomedicine.

Pengamatan morfologi β-tcp yang disintesis dari cangkang kerang hijau

β-Tricalcium Phosphate (β-TCP) is a biomaterial commonly used in dentistry as a bone substitute. β-TCP is a calcium phosphate compound where β-TCP requires a calcium source to be synthesized. The source of calcium could be obtained from green mussel shells because they have high calcium carbonate (CaCO3). The purpose of this study was to determine the effect of sintering temperature on the morphology of β-TCP synthesized from green mussel shells. The method of this research is an exploratory research conducted by knowing the effect of sintering temperature on the morphology of β-TCP synthesized from green mussel shells. The results of the synthesis were tested for characterized with a Scanning Electron Microscope (SEM). In the SEM characterization results, the higher the sintering temperature used in the sample, the more fused and resulting larger particle size. β-TCP with sintering temperature variations of 950℃, 1.000℃, 1.050℃ and 1.100℃ has a morphology consisting of small particles of irregular shape and bonding to each other to form micropores. The conclusion in this study is that the higher the sintering temperature applied, the more the particles coalesce and form larger particles.

Use of hydroxyapatite powder derived from green mussel shell wastes for coating on AISI 316L

This study aimed to determine the effect of preheating AISI 316L metal substrate on the adhesion strength of the hydroxyapatite (HA) layer derived from green mussel shell waste deposit by the flame spray method. The deposition experiment was carried out in a spray gun with a distance of 10 mm with preheat of 200°C, 400°C, and 600°C. A sample without preheating was also a comparison. An optical microscope was for assessing the thickness and uniformity of the HA surface layer. The adhesion strength of the coatings followed the ASTM D4541 Standard. As a result, the adhesion strength of the HA coating increases with increasing preheat temperature. However, using Image-J software, at an initial heating temperature of 600 °C, the adhesion strength of the coating changed from adhesion- to adhesion-cohesive strength, leading to an increase in coating strength noted. The result can add knowledge to understanding the flame spray method for the HA-coated from green mussel shell waste on AISI 316L. The results obtained can increase knowledge to understand the method of flame spraying on HA-coated green mussel shell waste on AISI 316L. Preheat temperature, spray gun shooting distance, HA powder release, oxygen gas pressure, and LPG gas pressure can be used as references.

Effect of withdrawal speed variations on coating quality of electrophoretically deposited biogenic hydroxyapatite on AISI 316L

The use of biogenic waste for multipurpose materials has started to be encouraged in the current decade to support the world’s environmental sustainability campaign. Biogenic calcium phosphate-derived materials are potential biomaterials because of their similar properties to human bone. This paper used hydroxyapatite (HAp) powder derived from green mussel shells (Perna viridis) to coat the AISI 316L metallic implant material using the electrophoretic deposition coating method. In this study, the post-deposition withdrawal speeds of 5mm/s, 3mm/s, and 1mm/s were varied to find their effect on the quality of the HAp coating layer. Crystallography, micrography, and image processing were used to characterize the coating and calculate the percentage of HAp layer and cracks. The crystallographic data shows the HAp peaks, which conclude that the green mussel shells HAp successfully coat the substrate and can be used to coat the AISI 316L implant material. However, the appearance of the substrate crystal peaks and color difference on high withdrawal speed specimens conclude that the coating layer has cracks. In conclusion, the emergence of cracks can be minimized using 1mm/s post-deposition withdrawal speed and eventually produce high-quality HAp coating.

Ultrasound-assisted extraction of phytochemicals from green coconut shell: Optimization by integrated artificial neural network and particle swarm technique

This study employs artificial neural network (ANN) and particle swarm optimization (PSO) to maximize antioxidant and antimicrobial activity from green coconut shells. Phytochemical analysis was carried out on the extract obtained from ultrasound-assisted extraction performed at different combinations of time (10, 20, and 30 min), temperature (30, 35, and 40 °C), and the ratio of solid-solvent (1:10, 1:20, and 1:30 g/ml). The presence of these bioactive compounds exhibits antimicrobial and antioxidant activities. Quantitative analysis showed that the total phenolic compounds ranged from 7.08 to 33.46 mg GAE/g, flavonoids ranged from 2.09 to 28.46 mg QE/g, tannins ranged from 70.5 to 141.09 mg TAE/g, and antioxidant activity of 49.98–66.1 %. The FTIR analysis detected the presence of CO, O–H, and C–H bonds. The optimized condition of ultrasound-assisted extraction (UAE) was compared with the optimized condition of the microwave. The result of ultrasound-assisted extraction was observed to be better than microwave-assisted extraction.

Potential Use of Natural Zeolite and Green Shells in Biodiesel Production

As energy needs continue to rise in line with the use of fossil energy, which is difficult to renew, other alternative energy is needed to continue to meet current and future energy needs. One alternative energy that is easy to produce is biodiesel. One of the raw materials that is quite widely used and developed in making biodiesel is waste cooking oil. According to statistical data, Indonesia can produce waste cooking oil of 5.06 tons per year. Waste cooking oil contains free fatty acids that can be processed into biodiesel using the help of heterogeneous catalysts. One source of heterogeneous catalysts that can be used is CaO, which can be found in green shells, where the CaO contained can be taken through the calcination process of green shells. To maximize the waste cooking oil processing process, an adsorption pretreatment process can be carried out. The adsorption process is carried out to reduce the FFA levels and acid numbers contained in waste cooking oil so that the transesterification reaction process can run optimally. Natural zeolite can be used as a good adsorbent to purify and lower the acid number of waste cooking oil. This study aims to find the best operating conditions in producing biodiesel made from waste cooking oil with a pretreatment adsorption process using natural zeolite and green shell catalyst through the factorial design method, where from the results of the research carried out, it was found that the density and viscosity met the SNI, the biodiesel yield produced was 45-65% and the cetane number test on samples 3 and 7 was 34,7 and 34,2 which did not meet the SNI. The operating temperature and amount of catalyst used in the biodiesel manufacturing process produce biodiesel with good density, viscosity, and yield, namely at a temperature of 60°C and a catalyst content of 6 grams. The operating temperature of 65°C produces a smaller density because the boiling point of alcohol is 64.7°C, so the reaction process is not perfect.

Almond (Prunus amygdalus L.): A source of revitalizing health and its therapeutic application

Nature is the source of some exquisite food products, having a wondrous amalgam of beneficial bioactive compounds with diverse health effects, often unattainable in synthetic products. Almond is a one of the valuable health benefiting food, from the Rosaceae family, have long been known as a source of essential nutrients; nowadays, they are in demand as a healthy food with increasing popularity for the general population and producers. They are native to the region which extends from India to Persia; the almond tree had spread to east and west of its native region thousands of years before Christ. Bitter and sweet almond are its types. It consists of Kernel or meat, mid shell and outer green shell. They are a rich source of vitamin E, dietary fiber, B-vitamins, proteins, calcium, magnesium, mono-unsaturated fats and phytosterols. This is also one of the classical Unani drugs prescribed by Hakeems in different body ailments. In Unani literature, it is mainly described as Muqawwī-i-dimāgh (Brain tonic), Murattib-i-dimāgh (Brain demulcent), Mulayyin (Laxative), Muqawwī-i-bāh (Aphrodisiac) and Mughadhdhi (Nutritious) etc. This is a review paper which discuss morphology, pharmacological action, ethno-medicinal and therapeutic uses of this medicinal plant in perspective of Unani medicine. This review has been done through online searches of databases such as PubMed, Google Scholar, Embase, science direct and hand search for classical textbook available in libraries. It concluded that Almond is one of the best herbal medicines in treatment of Palpitation, Amnesia, Hyperlipidemic, Sexual Debility, Dysmenorrhea, as it possess the following properties Antidiabetic, Antifungal, Anticancer, Antioxidant, Antihypertensive, Anti-inflammatory, Immunity enhancer, Hepato-protective Neuro-protective.
 Keywords: Unani System of Medicine, Almond, Badam Shireen, Neuroprotective, Immunobooster, Muqawwī-i-dimāgh

Application of green mussel (Perna viridis) shells hydroxyapatite on osteocalcin levels and osteoblast cells in rabbit femur bone defect.

Bovine hydroxyapatite (HA) used for bone grafts is relatively expensive, necessitating the development of alternative sources. Alternative HA materials derived from green mussel shells with smaller molecular sizes are inexpensive and abundantly available throughout Indonesian waters. The purpose of this study is to investigate the effect of green mussel shells HA on bone healing. This post-test-only experimental research used male rabbits with femoral defects divided into three groups randomly: K (no treatment), P1 (bovine HA treatment), and P2 (green mussel shell HA treatment). The osteocalcin level was assessed biochemically while osteoblast cells were histopathologically at the second, fourth, and sixth weeks. Statistic tests were used to assess differences between groups and periods with statistical significance P<0.05. Nine rabbits in each group showed significant differences between groups K, P1, and P2 in term osteocalcin levels at week 2 (2.60, 4.53±0.12, 4.47±0.23; P=0.046), week 4 (5.13±0.12, 8.53±0.12, 7.47±0.12; P=0.025), and week 6 (8.20, 11.93±0.23, 10.93±0.31, P=0.023), while in term osteoblast cells only at week 6 (16.33±3.46, 26.10±3.52, 30.40±3.29; P=0.006). The osteocalcin level and osteoblast increased significantly between groups K and P1/P2 from the initial trial until the last week. Osteoblast cells in the groups P1/P2 increased significantly, especially at week 6. Green mussel shell HA has the biochemical effectiveness of osteocalcin and can increase osteoblast cells comparable to bovine HA, which can enhance bone healing.

The Usability of Extracts of Walnut Green Outer Shell for In vitro Surface Sterilization of Rosehip (Rosa canina L.) Plant

In this study, it was aimed to determine the efficiency ratios of extracts obtained from walnut outer green shell at different concentrations in order to provide surface sterilization in in vitro propagation of rosehip plant. Axillary buds taken in June were used as explants. After surface sterilization of the explants with walnut outer shell extract, they were cultured in MS0 nutrient medium. The lowest contamination rate on the 5th and 7th days was observed in the control and 20K groups. In addition, the highest number of shoots per explant was obtained in the 20K group. Uncontaminated explants were subcultured to MS0 medium containing 1.0 mg /L IBA + 1.0 mg /L BAP and 1.0 mg/L BAP + 1.0 mg/L NAA at the end of the 7th day. Rooting was not observed in the explants due to browning. In the in vitro propagation of plants, extract (20K) obtained from oven-dried walnut shell can be used instead of chemical sterilant.