Green Seaweed Ulva Lactuca Research Articles

Antifouling activity of Malaysian green seaweed Ulva lactuca and its isolated non-polar compound

Marine natural products especially seaweeds have gained much attention to combat biofouling. Ulva lactuca was determined for its antifouling activity and characterized the isolated non-polar metabolite involved. The methanolic crude extract (MCE) of U. lactuca was screened using crystal violet assay against biofilm-forming bacteria Pseudomonas aeruginosa and was further tested on laboratory and field tests. Then, it was fractionated and isolated using Liquid-Liquid Fractionation (LLE) and Column Chromatography (CC). The isolated compound was characterized using Liquid Chromatography-Mass Spectrometry (LC-MS), Nuclear Magnetic Resonance (NMR), and Fourier Transform-Infrared Spectroscopy (FTIR). The current study showed that the growth of biofilm produced by P. aeruginosa was inhibited by MCE at concentrations of 0.0156 mg/mL. The laboratory test indicated UL5% demonstrated a higher bacterial reduction of bacterial colonies with 1.903 × 106 CFU/mL better than blank paint. According to the field test, crude panels of UL5% were successful in reducing the settlement of fouling organisms due to less macrofouler growth compared to blank paint. The isolated compound A4 was identified as hexadecanoic acid (C16H32O2) through NMR with a molecular mass of 256 g/mol detected using LC-MS. The characterization through FTIR obtained functional groups consisting of CH3, CH2, C=O, and OH. Therefore, U. lactuca produced hexadecanoic acid as one of the promising compounds from the seaweed group as an eco-friendly antifouling agent.

Structure, laser scattering, optical properties, adsorption, and dye removal studies by Ulvan extracted from the green seaweed Ulva lactuca

Ulvan, a sulfated polysaccharide polymer derived from green seaweed, was extracted from the marine alga Ulva lactuca and characterized by static laser scattering technique and showed a molecular weight of 43513 g/mol. Using a precipitation method, ulvan film was fabricated with a thickness of 2 µm. X-ray diffraction studies indicated the semicrystalline nature of ulvan. The optical characteristics of the ulvan film were computed and revealed a direct energy gap of 3.39 eV, with the dispersion energy and single oscillator energy being determined to be 13.783 and 7.425 eV, respectively. Subsequently, ulvan was used as an adsorbent to extract methylene blue dye from a water solution. After 30 min, the dye had been removed to an 86.8 % proportion. Moreover, TD-DFT simulation was used for the isolated ulvan molecule to estimate several physio-chemical parameters from HOMO and LUMO energies as well as to examine the electron density and electrostatic potential diagrams. Furthermore, additional significant polymeric physical properties were estimated for the isolated ulvan molecule. Furthermore, TD-DFT/CASTEP simulation was used to theoretically estimate the optical properties, and the results were consistent with the experimental findings. Lastly, research on the molecular adsorption of ulvan and methylene blue revealed a positive interaction between the two.

Immunomodulatory potential of sulfated xylated rhamnoglycan from an edible green seaweed Ulva lactuca: Regulation of cytokine expression in CALU-1 cells and prospects for therapeutic applications

Green seaweeds, particularly Ulva lactuca, commonly known as sea-lettuce, from the Ulvaceae class, constitute a significant proportion of the global edible seaweed population and serve as a rich storehouse of pharmacologically active compounds, including polysaccharides. In this study, a sulfated (1 → 4) linked xylated rhamnoglycan, (ULP-2), was extracted from Ulva lactuca. ULP-2 displayed significant immunomodulatory properties by modulating the expression of inflammatory cytokines in lipopolysaccharide (LPS)-induced CALU-1 non-small-cell lung cancer (NSCLC) cells. Treatment with ULP-2 at a concentration of 125 μg/mL resulted in a notable downregulation (63%, p < 0.05) of interferon (IFN)-γ gene expression compared to LPS-treated cells. Furthermore, the overexpression of tumor necrosis factor (TNF)-α and interleukin (IL)-1β was significantly attenuated (p < 0.05) with ULP-2 treatment (125 μg/mL), showing substantial downregulation to 0.82% and 1.81%, respectively, compared to LPS-induced cells (29.28% and 94.89%, respectively). Additionally, a noteworthy 1.3-fold reduction in IL-33 expression was observed following the administration of ULP-2 at 125 μg/mL. An upregulation of IFN-α expression, was evident in LPS-induced CALU-1 cells following treatment with ULP-2 within the concentration range of 31.25–125 μg/mL. The structure-activity study reinforced the significance of the presence of polyanionic sulfate and the (1 → 4) linkage in ULP-2 for binding to their respective receptors, influencing the orientation and spatial arrangement of the molecule, and subsequently triggering intracellular signaling pathways linked to the activation of innate immunity. Consequently, this study highlights ULP-2 as a promising natural compound derived from edible seaweed with potential applications in the treatment of inflammatory disorders and immune enhancement.

Phytochemical Constituents and Antimicrobial Activity of Marine Green Seaweed Ulva lactuca

The aim of this study is to determine the presence of Ulva seaweeds, possibly by analyzing the quality of seaweed powder extracts and some organic solvents. It belongs to the order Ulvaales. Ulva lactuca is a widespread macro algae growing the Mediterranean coast phylum Chlorophyta, commonly known as “sea lettuce”. Collected from the Gulf of Mannar Tamil Nadu, India. Dry the Ulva seaweeds and grind it in a food processor until it becomes a fine powder. The powder is dried in an oven at 600c for 24 hours. Alkaloids, Flavonoids, Saponins and Tannins. dried seaweed nutrients rich in energy 252.72kcal/mg/gm, carbohydrates 49.63mg/gm, less protein 12.21mg/gm, fat 1.04mg/gm less than gm, high crude fiber, ash15.8mg/g, high moisture 21.74mg/g. in the UV –visible spectrum of seaweed extract TLC analysis 200-800nm found a high value of0.925, to0.477, GC-MS RF value of8.014, and FTIR analysis of seaweeds Ulva found a high value of 618 to 3525cm-1 and HPLC showed retention time 2.213- 3.730. The antimicrobial studies maximum inhibition zone, minimum concentrations, minimum inhibition zone and maximum activity of Klebsiella pneumoniae, are methanol extract 10mm, Staphylococcus aureus, ethyl acetate extract11mm, Candida albicans 10.5mm were respectively.

A Comparative Study on Macronutrients Content and Antioxidant Activity of Four Wild and Cultivated Seaweeds from the Moroccan Atlantic Coast

ABSTRACT: Extensive harvest of seaweeds in Morocco has caused overexploitation of these resources. Four Moroccan common seaweed species, wild and cultivated, were studied to assess the cultivated species' potential in comparison to their wild counterparts. The macronutrient composition, total phenolic compounds (TPC), as well as potential antioxidant activity (DPPH and FRAP), were established. Seaweeds contained a wide range of proteins (5–16% of DW), lipids (1–3% of DW), carbohydrates (20–59% of DW), and total polyphenols (3-6 mg EAG/g of DW). Red seaweeds contained high levels of polysaccharides (47–59% of DW), mainly the cultivated Gigartina pistillata, and important amounts of proteins, especially Gelidium sesquipedale (14–15% of DW), whereas the cultivated type was richer in proteins. Gracilaria gracilis and wild Gelidium sesquipedale are characterized by notable antioxidant activity, particularly the cultivated Gracilaria gracilis (TPC: 6.807 mg GAE/g of DW; low values of IC50 of DPPH: 53.863μg/mL and FRAP: 67.033μg/mL). Besides, the green seaweed Ulva lactuca is poor in carbohydrates but contains essential amounts of proteins and phenolics. This work highlights the significant potential of cultivating Moroccan seaweeds through algaculture to achieve more sustainable production with enhanced nutrient and bioactive molecule content.

In Vitro Assessment of Antiviral, Antimicrobial, Antioxidant and Anticancer Activities of Ulvan Extracted from the Green Seaweed Ulva lactuca

Marine macroalgae contain a variety of bioactive substances that have many different uses. Ulvan, a sulfated hetero-polysaccharide isolated from Ulva lactuca, has been described and investigated for its antioxidant, antiviral, antibacterial, and cytotoxic action against the cell lines PC-3 and A-549. The yield of polysaccharides was 26.53 ± 0.32. (Algal dry weight) with 2.23% uronic acid. According to HPLC estimates, the polysaccharide isolated from Ulva lactuca contains the neutral sugars glucose, rhamnose and galactose, as well as fructose, and melibiose. The FT-IR spectrum of ulvan revealed bands at 1227.73 cm− 1 and 1333.97 cm− 1, respectively, indicative of sulfate esters and uronic acids. This study came to the conclusion that Ulva lactuca ulvan (ULU) contains beneficial elements that can stop the development of cancer cells. The lowest viability of PC-3 cells, which occur at the highest concentration of ULU (500 µg/ml), was 17.58% with an IC50 of 237.02 ± 6.84 µg/ml, while the lowest viability of lung carcinoma cells was 13.91% with an IC50 of 124.38 ± 4.78 µg/ml. This indicates that the cytotoxicity against the prostate cancer cell line was lower than that against the lung carcinoma cell line. Although ULU has no activity against adenovirus, it had antiviral efficacy against HAV-10. ULU has been shown to have antibacterial efficacy against pathogenic bacteria, including Staphylococcus aureus ATCC25923 Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29,212, Pseudomonas aeruginosa ATCC 9027, Klebsiella pneumonia ATCC 13,883, Pseudomonas fluorescent ATCC13525 and Streptococcus agaloctiae ATCC13813. The polysaccharide, meanwhile, has limited antioxidant action.

Impact of thermal cooking processes on organochlorine pesticide residues (OCPs) in the edible green seaweed, Ulva lactuca, and associated human health risk assessment

Seaweeds are emerging natural food items in different countries. However, they are known to be potentially contaminated by chemical residues. This study examined the impact of domestic cooking methods such as boiling, steaming, and microwave cooking on reduction of organochlorine pesticide (OCP) level in edible green seaweed Ulva lactuca. Thermal cooking processes significantly influences the OCP residues in U. lactuca. The OCP residue concentration decreased after boiling, steaming, and microwave cooking compared to raw seaweed. The total OCP residue concentration in raw seaweed was 0.707 mg/kg, which reduced to 0.168, 0.178, and 0.241 mg/kg after boiling, microwaving, and steam cooking, respectively. Aldrin, endrin, DDT, and endosulfan were predominant OCPs in raw and processed seaweeds. Health risk due to OCP residues was evaluated using food consumption data, acceptable daily intake, and safety indices such as hazard quotient (HQ) and hazard index (HI). The HQ and HI values did not exceed the unit value (HQ, HI < 1), indicating that the level of OCP residues in U. lactuca does not pose any appreciable risk to the consumers. Therefore, the research finding establishes that different thermal cooking processes reduce the OCP residue concentration in U. lactuca.

Assessing pharmaceuticals in the green seaweed Ulva lactuca through a multi-residue UHPLC-ToF-MS strategy

Seaweeds have become an important asset in several sectors, including the food and feed industries, cosmetics, and pharmaceuticals, among others. Whether harvested or reared, interest in algae has been growing worldwide due to the resources they offer, including proteins, vitamins, minerals, carbohydrates, essential fatty acids, and dietary fiber, as well as sources of biologically active compounds. However, given their morphology and physiology, as well as their harvest and cultivation environments, algae are prone to the presence of hazards, including pharmaceuticals taken up from the water. Thus, to ensure human and animal safety as well as environmental health, monitoring is essential. Therefore, this work describes the development and validation of a sensitive screening and confirmatory analytical method based on ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (UHPLC-ToF-MS). This multi-residue method enables the determination of 62 pharmaceuticals distributed between 8 therapeutic classes and was fully validated according to Commission Implementing Regulation (EU) 2021/808.

Aqueous homogenate of fresh Ulva lactuca for ameliorating nutrient deficiency—A nutraceutical alternative to using whole seaweeds

The green seaweed Ulva lactuca has food value but owing to the presence of high fibers in the form of soluble polysaccharides, its organic matter has reportedly low digestibility. However, owing to high elemental content and presence of bioactive molecules, a strategy to make chemical-free extract was conceived to partition it into mineral rich fraction by homogenizing the fresh seaweed with water, followed by filtering and lyophilizing the extract into powder form (LPAH), thus also lowering the carbohydrate (12 g 100 g−1) and protein (4.32 g 100 g−1) content in it compared to dried whole seaweed. The LPAH was mineral rich and a modest dose of 7.2 g day−1 is capable of fulfilling about 20, 4, 98 and 33 % of the recommended daily allowances of sodium, potassium, iron and boron, respectively. Using the state-of-the-art analytical techniques, the LPAH was characterized and was found to be rich in several known bioactive compounds. It contained phenols (179.99 mg GAE 100 g−1), flavonoids (47.88 mg QE 100 g−1), carotenoids (7.58 mg g−1), and ascorbate (2046.43 μg g−1). The dosage contemplated could also suffice 18 % of ascorbic acid requirement of the human body. The LPAH also showed antioxidant activities such as, DPPH (601.65 μg TE g−1), ABTS (89.77 μg TE g−1), FRAP (154.31 μg TE g−1), TAC (54.57 μg AA g−1). 26 metabolites and 16 amino acids were identified and quantified using GCMS and HPLC, however, none of the amino acids was sufficient enough to act as a protein substitute or supplement. Using LC-HRMS-MS/MS, 43 known bioactive compounds such as Dioncophylline C, hericenone B, alpha-hydroxy-N-desmethyltamoxifen, kni-102, dibenzo pentapehene, proglumide, capsite were identified in the methanolic extract of LPAH, suggesting its nutraceutical value for functional food applications.

Effect of seasonality on the amino acid and monosaccharide profile from the green seaweed Ulva lactuca cultivated in plastic sleeves onshore (Mikhmoret, Israel)

Effect of seasonality on the amino acid and monosaccharide profile from the green seaweed Ulva lactuca cultivated in plastic sleeves onshore (Mikhmoret, Israel)

The Bioremediation Potential of Ulva lactuca (Chlorophyta) Causing Green Tide in Marchica Lagoon (NE Morocco, Mediterranean Sea): Biomass, Heavy Metals, and Health Risk Assessment

Pollution by heavy metals is one of the most serious issues in the world, and the principal sources are manufacturing, fertilizers, mining, pesticides, transport, and wastewater discharge. In this study, the presence of macroelements (Nitrogen (N) and Phosphorus (P)) and heavy metals (Chromium (Cr), Nickel (Ni), Cadmium (Cd), Iron (Fe), Zinc (Zn), Lead (Pb), Cooper (Cu), and Manganese (Mn)) in the green tide caused by the green seaweed Ulva lactuca from Marchica lagoon (NE-Morocco, Mediterranean) was evaluated. The mean values of P and N in the algae were 1773.33 mg·kg −1 and 44.4 g·kg −1, respectively. The heavy metals have mean values following this descending sequence: Fe &gt; Mn &gt; Zn &gt; Cu &gt; Ni &gt; Pb &gt; Cr &gt; Cd. This finding has suggested that Ulva has the strongest affinity to Fe and Mn among the metals examined. Mn, Fe, and Pb recorded the highest bioconcentration factors (BCFs), which were 1687, 1656, and 1643, respectively. Regarding their contribution to the Recommended Dietary Allowance (RDA), Fe showed a contribution of 13.58% to the RDA, while that of Mn varied between 4.42 % and 28.42%. The health risk assessment did not indicate any hazard related to the ingestion of Ulva lactuca. Therefore, this study suggests that Ulva lactuca in the Nador lagoon has the potential to bioaccumulate heavy metals and mitigate eutrophication.

Ulvan from green seaweed Ulva lactuca: Optimization of ultrasound-assisted extraction, structure, and cytotoxic activity

An ulvan was extracted from green seaweed Ulva lactuca by ultrasound-assisted extraction. The extraction conditions were optimized by response surface methodology. The optimal conditions were extraction temperature at 84.75 °C, extraction time of 30.51 min, solvent to material ratio of 60.51 mL/g to achieve the yield of 22.5%. The ulvan is composed of repeated sequences of three disaccharides: →4)-β-D-Glucuronic acid(1→4)α-L-Rhamnose-3-sulfate(1→, →4)α-L-Iduronic acid(1→4)α-L-Rhamnose-3-sulfate(1→, and →4)α-D-Xylose-2-sulfate(1→4)α-L-Rhamnose-3-sulfate(→. The ulvan showed cytotoxic activities against five human cancer cell lines, including human hepatocellular carcinoma, human breast cancer, human cervical cancer, human colorectal adenocarcinoma and human undifferentiated thyroid carcinomas.

Process and technoeconomic analysis of bioethanol production from residual biomass of marine macroalgae Ulva lactuca

In the present work, the residual biomass of the green seaweed Ulva lactuca was chosen as feedstock to undergo separate hydrolysis and fermentation process to produce bioethanol. The hydrolysis process was optimized for cellulase, biomass, temperature, and time conditions. The maximum yield of fermentable sugars was 13.48 mg/mL. The recovered hydrolysate was subjected to fermentation using Saccharomyces cerevisiae. The bioethanol produced was subjected to gas chromatography coupled mass spectrometry analysis to determine the presence of ethanol. The technical performance and economic feasibility of the bioethanol production from U. lactuca were evaluated using the lab-scale data obtained for optimized conditions. The plant capacity was 10 MT/day of bioethanol production. The plant's capital investment and annual operating cost were 3.18 M$ and 0.86 M$ respectively. The total annual revenue of the plant was 1.41 M$. The minimum selling price of bioethanol was 0.47 $/kg. The ROI, payback period, IRR and NPV of the plant were 16.99 %, 5.89 years, 11.57 % and 291,000 $ respectively. The utilization of residual biomass for biofuels helps to develop an economic and environmentally sustainable plant.

Study On Phytochemical and Antioxidant Properties of Padina gymnospora and Ulva lactuca

Seaweed constitutes one of the commercially important living renewable resources in the marine ecosystem.Seaweeds have antimicrobial, antioxidant, and anti-inflammatory properties. The aim of the study is to estimate the phytochemicals and antioxidant activity of Padina gymnospora and Ulva lactuca. The antioxidant properties and phytochemical analysis of Padina gymnospora and Ulva lactuca collected from the Gulf of Mannar during the low tide period were analyzed.The study's objective is to analyze the phytochemical and antioxidant activities using methanol as solvent. Antioxidant activities were determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay treated with methanol and Phytochemical analysis carried out by Folin-Ciocalteu reagent. Antioxidants and phytochemicals in seaweeds provide healthpromoting effects and protect against non-communicable diseases such as Cancer, arthritis, diabetes, obesity, etc. The results reveal that antioxidant activities were higher in Padina gymnospora in methanol solvent than in Ulva lactuca. The percentage of inhibition of Padina gymnospora and Ulva lactuca was 61.5 and 64.5 respectively. IC50 value of Padina gymnospora and Ulva lactuca was 60.4 and 60.6 respectively.The preliminary phytochemical analysis of Padina gymnospora and Ulva lactuca was found to contain Alkaloids, Steroids, Terpenoids, Glycosides. To know the efficiency of green seaweed Ulva lactuca with brown seaweed Padina gymnospora the study was carried out. The highest flavonoid content was recorded in brown seaweed Padina gymnospora (25.5±0.02mg REg-1) compared toUlva lactuca (19.32±0.5mg REg-1). The total phenolic content was highest in Padina gymnospora (3.7±0.06mg GAEg-1) whereas Ulva lactuca contained (2.81±0.3mg GAEg-1). Therefore, the present work recommends consuming seaweeds in our regular diets to have a healthy lifestyle. From the current study of phytochemical and antioxidant rich seaweeds can be incorporated in the formulation of drugs used for treating various non- communicable diseases prevailing in the present era. Keywords: , , ,

Optimization of Ultrasound-assisted Extraction of Ulvan from Green Seaweed Ulva lactuca

In this paper, the extraction conditions of ulvan from green seaweed Ulva lactuca by using Ultrasound-assisted extraction method were optimised. Experience was designed using Box-behnken model with experient areas as following: extraction temperature (X1: 50–90 °C), extraction time (X2: 20–40, min) and solvent-to-material ratio (X3: 50/1–70/1, v/w), objective function (Y: extraction yield) was required to get maximum value. The result showed that the optimization conditions for extraction were: extraction temperature 84.28 °C, extraction time 30.59 min with 60.21/1 (v/w) as solvent-to-material ratio, and the expected objective function Y is 22.86% based on dried seaweed weight.

Utilization of cellulose and ulvan from the green seaweed Ulva lactuca in the development of composite edible films with natural antioxidant properties

Cellulose and ulvan were extracted sequentially from the green seaweed Ulva lactuca and were used for the development of edible films with natural antioxidant properties. The composite films were composed of cellulose, polyvinyl alcohol, glycerol as plasticizer and different ulvan concentrations. The interaction between ulvan and film components was described by FT-IR analysis, which confirmed the formation of hydrogen bonding interactions between cellulose and ulvan. The incorporation of ulvan into the cellulose films markedly increased film thickness, water solubility and water vapor permeability, whereas the oxygen permeability was decreased. Additionally, the composite films showed good barrier properties against UV and visible light. The moisture sorption of the films was modelized using Peleg's model which exhibited a satisfactory fitting to the experimental data. The thermal stability was markedly enhanced in the presence of high concentration of ulvan. Furthermore, the incorporation of ulvan to cellulose films enhanced its antioxidant properties as measured by total antioxidant assay (TAC), ferric reducing antioxidant power (FRAP), hydroxyl radical scavenging activity (HRSA) and iron chelating activity (ICA). The use of water and 3% acetic acid as food simulants indicated that the FRAP was higher in case of water, while the opposite trend was observed for the HRSA and ICA. Overall, the developed cellulose/ulvan films could have potential uses in food packaging to promote the shelf-life of different food products.

Effect of the Lipid Complex from Green Seaweed Ulva lactuca Linnaeus, 1753 on the Biochemical Parameters of Blood Plasma and the Liver in Experimental Dyslipidemia

Effect of the Lipid Complex from Green Seaweed Ulva lactuca Linnaeus, 1753 on the Biochemical Parameters of Blood Plasma and the Liver in Experimental Dyslipidemia

Green seaweed Ulva lactuca, a potential source of bioactive peptides revealed by in silico analysis

The green seaweed Ulva lactuca is found in coastal areas. Potential bioactive peptides have a wide range of uses, including antibacterial, antifungal, antiviral, alpha-amylase inhibitory, antiamnestic, ACE inhibitory, immunomodulatory, anticancer, antioxidative, contracting, anti-inflammatory, and more. This study's major goal was to use in-silico methods to reveal the availability and abundance of putative bioactive peptides in the RuBisCO protein of Ulva lactuca seaweed. The protein sequence was obtained using the UniProtKB database. The peptide sequences were produced by using the BIOPEP-UWM enzyme activity tool. The peptides were ranked using PeptideRanker, and the toxicity profile was assessed using ToxinPred. For the first time, the current in-silico investigation depicts the pharmacological characteristics of Ulva lactuca by focusing on the RuBisCO protein. Numerous bioactive peptides with inhibitory activity against the angiotensin-I converting enzyme (ACE), dipeptidyl peptidase IV, antioxidant, dipeptidyl peptidase III, renin, antithrombotic, antiamnestic, α-glucosidase, anti-inflammatory, hypolipidemic activity, and ion flow regulation were produced by the in-silico proteolysis of rubisco using 18 proteases. Based on the cutoff score >0.75, pancreatic elastase liberated the most bioactive peptide sequences from the rubisco compared to other proteases. Additionally, PeptideRanker's findings showed that a number of di-, tri-, and/or polypeptides produced from the RuBisCO have a great deal of potential to be used as bioactive peptides. These studies open up new opportunities for these bioactive peptides in the fields of pharmacy, medicine, the food sector, and marine biotechnology.

Pharmacological Applications of the Green Seaweed Ulva lactuca

Pharmacological Applications of the Green Seaweed Ulva lactuca

Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aimed to produce bioactive ulvan oligosaccharides via enzymatic hydrolysis from green seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, were used to produce multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, and xylanase, to degrade the ulvan extract. An ultrafiltration system was used to separate the enzymatic hydrolysate to acquire the ulvan oligosaccharides (UOS). The characteristics of the ulvan extract and the UOS were determined by yield, reducing sugar, uronic acid, sulfate group, and total phenols. The FT-IR spectrum indicated that the ulvan extract and the UOS presented the bands associated with O-H, C=O, C-O, and S=O stretching. Angiotensin I converting enzyme (ACE) inhibition and antioxidant activities in vitro were evaluated in the ulvan extract and the UOS. These results provide a practical approach to producing bioactive UOS by microbial enzymatic hydrolysis that can benefit the development of seaweed-based products at the industrial scale.