Antifouling activity of Malaysian green seaweed Ulva lactuca and its isolated non-polar compound

Abstract

Marine natural products especially seaweeds have gained much attention to combat biofouling. Ulva lactuca was determined for its antifouling activity and characterized the isolated non-polar metabolite involved. The methanolic crude extract (MCE) of U. lactuca was screened using crystal violet assay against biofilm-forming bacteria Pseudomonas aeruginosa and was further tested on laboratory and field tests. Then, it was fractionated and isolated using Liquid-Liquid Fractionation (LLE) and Column Chromatography (CC). The isolated compound was characterized using Liquid Chromatography-Mass Spectrometry (LC-MS), Nuclear Magnetic Resonance (NMR), and Fourier Transform-Infrared Spectroscopy (FTIR). The current study showed that the growth of biofilm produced by P. aeruginosa was inhibited by MCE at concentrations of 0.0156 mg/mL. The laboratory test indicated UL5% demonstrated a higher bacterial reduction of bacterial colonies with 1.903 × 106 CFU/mL better than blank paint. According to the field test, crude panels of UL5% were successful in reducing the settlement of fouling organisms due to less macrofouler growth compared to blank paint. The isolated compound A4 was identified as hexadecenoic acid (C16H32O2) through NMR with a molecular mass of 256 g/mol detected using LC-MS. The characterization through FTIR obtained functional groups consisting of CH3, CH2, C=O, and OH. Therefore, U. lactuca produced hexadecenoic acid as one of the promising compounds from the seaweed group as an eco-friendly antifouling agent.