Myzus Persicae Research Articles

In Vivo Bioassay of the Repellent Activity of Caraway Essential Oil against Green Peach Aphid.

An in vivo dual choice bioassay with white cabbage as a host plant was used to determine the repellent effect of three different accessions of caraway (Carum carvi L.) essential oils (EOs) against the green peach aphid Myzus persicae (Sulzer). The dominant components of the EO were D-Carvone (47.3-74.4%) and D-limonene (25.2-51.9%), which accounted for 99.2-99.5% of the EOs determined by GC/MS. The EO with the highest D-limonene content (51.9%) showed the highest repellence (Repellency Index (RI) = +41%), which was stable up to 330 min. The incorporation of several surfactants with different hydrophilic-lipophilic balance values (from 12.4 to 16.7) with caraway EO caused a general inhibition of the repellent effect during the testing period (RI from +41% to -19%). Overall, the findings indicate that caraway EO could be used as a green peach aphid repellent, but more work is needed to formulate the EO into a ready-to-use product.

Host Records of Lipolexis oregmae Gahan (Hymenoptera: Braconidae: Aphidiinae) with Food Plants in India (A Review Article)

The present review article deals the association of Lipolexis oregmae Gahan with their aphid host and their food plants for the possible use in biological control programme. Aphids are small sap sucking plant bugs and most important groups of phytophagous insect because of their polymorphism, host alternative heterocious behaviour and reproductive habits, they cause serious problems on agricultural and horticultural plants even at low densities so their management is necessary. L.oregmae Gahan is very important aphid parasitoids and widely distributed throughout the Asia and considered as an important parasitoids for the biological control programme. It has been successfully introduced in Florida and other areas. It is recorded on 19 aphid species (Aphis (Toxoptera) aurantii, Aphis (Toxoptera) citricidus, Aphis (Toxoptera) odinae, Aphis craccivora, Aphis fabae, Aphis gossypii, Aphis nasturtii, Aphis nerii, Aphis ruborum longisetosus, Aphis solanella, Aphis spiraecola, Greenidae formosana, Liosomaphis himalayensis, Myzus avenae, Myzus (Nectarosiphon) persicae, Rhopalosiphum maidis, Rhopalosiphum nymphaeae, Sitobion avenae, Tuberolachnus salignu) in India on several specific host plants. Its maximum parasitization was recorded on Aphis gossypii infesting (23 host plants), followed by Aphis craccivora (19 host plants), Aphis nasturtii (16 host plants), Aphis spiraecola (13 host plants) and Myzus persicae (9 host plants).

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

AbstractSix patchouli (Pogostemon cablin Benth.) plants showing symptoms of viral infection were found in Brazil. Transmission electron microscopy revealed flexuous particles and characteristic pinwheel‐shaped cytoplasmic inclusions of potyvirus in sap and cells of symptomatic leaves. Potyvirus infection was confirmed through RT‐PCR, using universal primers followed by nucleotide sequencing. The obtained sequences, comprising part of the CP gene and the 3′ non‐translated region, showed 97.71%–97.87% identity with an isolate of bidens mosaic virus (BiMV). The nucleotide sequences of the CP, HC‐Pro and CI genes of BiMV isolates from patchouli plants were obtained through RT‐PCR with specific primers followed by nucleotide sequencing. The obtained sequences showed 95.78%–97.1% identities with BiMV isolates from Brazil. One BiMV isolate from a patchouli infected plant was sap‐transmitted to different plant species. Chenopodium amaranticolor exhibited local lesions, Bidens pilosa developed leaf mosaic, and Nicotiana benthamiana exhibited chlorosis and stunting. The BiMV isolate was transmitted from infected patchouli plant to B. pilosa using Myzus persicae aphids. This is the first confirmation of patchouli infected with BiMV in Brazil. Further studies are necessary to assess the incidence of this potyvirus in commercial patchouli crops, as well as the damage caused to plant development and the quantity and quality of essential oils produced.

Fatty alcohols, a minor component of the tree tobacco surface wax, are associated with defence against caterpillar herbivory.

Despite decades of research resulting in a comprehensive understanding of epicuticular wax metabolism, the function of these almost ubiquitous metabolites in plant-herbivore interactions remains unresolved. In this study, we examined the effects of CRISPR-induced knockout mutations in four Nicotiana glauca (tree tobacco) wax metabolism genes. These mutations cause a wide range of changes in epicuticular wax composition, leading to altered interactions with insects and snails. Three interaction classes were examined: chewing herbivory by seven caterpillars and one snail species, phloem feeding by Myzus persicae (green peach aphid) and oviposition by Bemisia tabaci (whitefly). Although total wax load and alkane abundance did not affect caterpillar growth, a correlation across species, showed that fatty alcohols, a minor component of N. glauca surface waxes, negatively affected the growth of both a generalist caterpillar (Spodoptera littoralis) and a tobacco-feeding specialist (Manduca sexta). This negative correlation was overshadowed by the stronger effect of anabasine, a nicotine isomer, and was apparent when fatty alcohols were added to an artificial lepidopteran diet. By contrast, snails fed more on waxy leaves. Aphid reproduction and feeding activity were unaffected by wax composition but were potentially affected by altered cutin composition. Wax crystal morphology could explain the preference of B. tabaci to lay eggs on waxy wild-type plants relative to both alkane and fatty alcohol-deficient mutants. Together, our results suggest that the varied responses among herbivore classes and species are likely to be a consequence of the co-evolution that shaped the specific effects of different surface wax components in plant-herbivore interactions.

Direct interaction between micronutrients and bell pepper (Capsicum annum L.), to affect fitness of Myzus persicae (Sulzer)

Direct interaction between micronutrients and bell pepper (Capsicum annum L.), to affect fitness of Myzus persicae (Sulzer)

Evaluating a new aphid biocontrol agent: The role of aphid density in modulating oviposition behaviour in the American hoverfly, Eupeodes americanus, and the aphid midge, Aphidoletes aphidimyza

AbstractHoverfly larvae are voracious predators of aphids but have limited dispersal capacity, so their survival depends critically on female selection of oviposition sites. For aphid control purposes, the ability of females to find small aphid colonies is especially valuable. In this study, the oviposition strategy and minimum aphid density triggering the oviposition of the American hoverfly, Eupeodes americanus (Wiedemann, 1830) (Diptera: Syrphidae), was compared with that of the aphid midge, Aphidoletes aphidimyza (Rondani, 1847) (Diptera: Cecidomyiidae), a predator commercially available in Canada as a reference. Oviposition behaviour was evaluated at densities of 0, 2, 5, 10, and 50 aphids per plant. This was tested on two economically significant plant/aphid systems: pepper plants infested by the green peach aphid, Myzus persicae (Sulzer 1776) (Hemiptera: Aphididae), and cucumber plants infested with the melon aphid, Aphis gossypii Glover 1877. Results showed that both predators lay their eggs in proportion to the size of the colony of aphids. Oviposition by E. americanus required a minimum density of 5 aphids on cucumber but only 2 on pepper, whereas for A. aphidimyza, it was also 5 aphids on cucumber, but 10 on pepper. The hoverfly laid more than twice as many eggs as A. aphidimyza on pepper. It also laid more eggs on pepper than on cucumber and the pattern is the opposite for A. aphidimyza. Therefore, the identity of the aphid‐host plant complex appears to modulate the oviposition of these predators. This study demonstrates the ability of E. americanus to respond to lower densities of aphids than A. aphidimyza early in the infestation on sweet pepper plants and shows that its oviposition rate is at least as effective as A. aphidimyza on cucumber. Consequently, these attributes of its oviposition behaviour indicate that E. americanus has great potential for use as an aphid biocontrol agent in these crops.

Effect of Weed Growth on the Incidence of Sucking Pests in Cucumber

Field experiments were conducted in Fayoum Governorate to evaluate the impact of weeds growing in cucumber fields on the incidence of sucking pests viz., Aphis gossypii (Glover), Bemisia tabaci (Gennadius), Nezara viridula (L.), Phenacoccus solenopsis (Tinsley), Thrips tabaci (Lindeman), Myzus persicae (Sulzer), Empoasca decedens (Paoli) and Empoasca decipiens (Paoli) during 2018 and 2019 Neglected cucumber fields, with a high density of weeds, were observed to harbour more pests 57.7 and 55.9%, respectively. Meanwhile, the cucumber free-weedy field hosted the lowest rate of occurrence for all insect pests represented by 42.3 and 44.1%, respectively. Neglected fields with abundant weeds provide a favourable habitat, leading to increased incidence. Understanding the relationship between weeds and pests is crucial for developing effective IPM strategies in cucumber.

Bazı bitkisel yağların şeftali yeşil yaprak biti Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae)’nin yaşam çizelgesi parameterleri üzerindeki etkileri

In this study, essential oils (EOs) of Citrus limon (L.), Citrus sinensis (L.) (Sapindales: Rutaceae), Allium sativum (L.) (Asparagales: Amaryllidaceae) and Brassica nigra (L.) (Brassicales: Brassicaceae) were evaluated for their insecticidal effects on the green peach aphid Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae). The lethal and sublethal effects of these EOs on M. persicae were studied under laboratory conditions. This study was conducted at Yozgat Bozok University, Faculty of Agriculture, Department of Plant Protection in 2023. The experiments were evaluated at different concentrations for 24 hours after treatment. The lethal concentrations (LC50, LC90) of the EOs were calculated based on the data obtained. The life table parameters of newly born aphids were studied at sublethal concentrations (LC40, LC30) of EOs, and these parameters were calculated using the Euler-Lotka equation. The results show that the mortality rate increases with growing concentration of essential oils. The lethal concentration (LC50) of essential oils were calculated to be 3.47, 4.37, 4.51, and 5.16 µL/L, respectively. The sublethal concentrations (LC40, LC30) of essential oils caused an increase in adult longevity, a decrease in fecundity of surviving aphids and intrinsic rate of increase. From the data obtained, the EOs of C. limon and C. sinensis were more effective than other EOs in the study. It was found that other essential oils (A. sativum and B. nigra) may also be effective against M. persicae, even if their effect is low.

Genome-wide identification of nucleotide-binding domain leucine-rich repeat (NLR) genes and their association with green peach aphid (Myzus persicae) resistance in peach

Resistance genes (R genes) are a class of genes that are immune to a wide range of diseases and pests. In planta, NLR genes are essential components of the innate immune system. Currently, genes belonging to NLR family have been found in a number of plant species, but little is known in peach. Here, 286 NLR genes were identified on peach genome by using their homologous genes in Arabidopsis thaliana as queries. These 286 NLR genes contained at least one NBS domain and LRR domain. Phylogenetic and N-terminal domain analysis showed that these NLRs could be separated into four subfamilies (I-IV) and their promoters contained many cis-elements in response to defense and phytohormones. In addition, transcriptome analysis showed that 22 NLR genes were up-regulated after infected by Green Peach Aphid (GPA), and showed different expression patterns. This study clarified the NLR gene family and their potential functions in aphid resistance process. The candidate NLR genes might be useful in illustrating the mechanism of aphid resistance in peach.

Spotlight on Eupeodes americanus: Oviposition and fertility under HPS- and full spectrum LED-extended photoperiod in northern greenhouses

In northern temperate regions, high-pressure sodium vapor (HPS) or light-emitting diode (LED) lights for supplemental lighting in winter greenhouses are used to extend the photoperiod and increase plant production. These different greenhouse light treatments influence crop pests and their natural enemies. In this study, different greenhouse light treatments were evaluated for their effect on the green peach aphid, Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae) and its predator the American hoverfly, Eupeodes americanus (Wiedemann, 1830) (Diptera: Syrphidae). In particular, the development of M. persicae and the oviposition and egg hatchability of E. americanus were studied under three light treatments in winter greenhouse: 1) short photoperiod of natural sunlight, and extended photoperiod using either 2) full spectrum LED or 3) HPS lights. The results showed that both extended photoperiod treatments significantly increased M. persicae population growth by 103–130 %, after 12 days compared to short photoperiod of sunlight. Compared to the winter short photoperiod of sunlight, extending the photoperiod did not affect the oviposition of female hoverflies in terms of fecundity, fertility, and niche use (i.e., position of eggs on the plant). Nevertheless, extended photoperiod significantly increased the egg hatchability of E. americanus by 9 % (HPS) and 16 % (LED) compared to short photoperiod of sunlight. This treatment had the highest proportion of unhatched eggs (13 %). Finally, LED lights decreased the proportion of unhatched eggs by 71 % compared to HPS lights. This study, therefore, confirms the potential of the American hoverfly as a biological control agent in winter greenhouses, both with or without artificial light.

MpADC, an l-aspartate-α-decarboxylase, from Myzus persicae, that enables production of β-alanine with high yield by whole-cell enzymatic catalysis

Backgroundβ-Alanine is a precursor of many important pharmaceutical products and food additives, its market demand is continuously increasing nowadays. Whole-cell catalysis relying on the recombinant expression of key β-alanine synthesizing enzymes is an important method to produce β-alanine. Nevertheless, β-alanine synthesizing enzymes found so far have problems including easy inactivation, low expression or poor catalytic activity, and it remains necessary to develop new enzymes.ResultsHerein, we characterized an l-aspartate-α-decarboxylase, MpADC, from an aphid, Myzus persicae. It showed excellent catalytic activity at pH 6.0–7.5 and 37 °C. With the help of chaperone co-expression and N-terminal engineering guided by AlphaFold2 structure prediction, the expression and catalytic ability of MpADC in Escherichia coli were significantly improved. Using 50 g/L of E. coli cells expressing the MpADC-∆39 variant cultured in a 15-L fermenter, 232.36 g/L of β-alanine was synthesized in 13.5 h, with the average β-alanine yield of 17.22 g/L/h, which is best known so far.ConclusionsOur research should facilitate the production of β-alanine in an environment-friendly manner.

Footprints of predatory lady beetles stimulate increased dispersal of aphid prey, but do not alter feeding behavior or spread of a non-persistently transmitted plant virus

Predators impact prey directly, through consumption, and indirectly, through non-consumptive effects that modify prey physiology and behavior in ways that affect survival and reproduction. Non-consumptive effects (NCEs) are well documented across prey taxa in response to different predator cues. However, for most prey species, both the mechanisms underlying NCEs and the impacts of NCEs on broader ecological processes are poorly understood. We addressed these knowledge gaps for an aphid prey species (Myzus persicae) by studying dispersal, in-leaf feeding behavior, and plant virus transmission in response to chemical cues deposited by a walking predator (Hippodamia convergens), a non-predator, non-competitor (Drosophila melanogaster), and an artificial feeding deterrent (2% mineral oil). We used this approach to better understand the specificity of M. persicae responses to chemical footprints of threatening and non-threatening organisms, as well as the magnitude of behavioral responses relative to a known deterrent. We found that chemical footprints deposited by H. convergens stimulated M. persicae to disperse from a suitable host (Brassica napus) at a rate equivalent to the 2% mineral oil positive control, while footprints of D. melanogaster did not modify M. persicae dispersal. Through electrical penetration graphing (EPG) recordings, we found that mineral oil stimulated aphids to make more probes, but footprint treatments did not significantly modify feeding behavior. In mesocosm-based virus transmission assays, H. convergens footprints and 2% mineral oil also stimulated increased dispersal, but this did not translate into increased transmission of turnip mosaic virus. We identified components of H. convergens and D. melanogaster footprints using gas chromatography and mass spectrometry and found that H. convergens footprints are chemically similar to footprints of related Coccinellidae, but distinct from D. melanogaster footprints. Our results provide evidence of specificity in M. persicae responses to predator footprints, which may help this prey species avoid costly dispersal behavior in response to cues from non-predators. We also provide ecological context by demonstrating that increased dispersal in response to predator cues does not always lead to increased virus transmission, as previously assumed, particularly when in-leaf feeding behaviors underlying virus acquisition do not change in response to predator cues.

Temporal and species-specific resistance of sugar beet to green peach aphid and black bean aphid: mechanisms and implications for breeding.

Sugar beet (Beta vulgaris ssp. vulgaris), a key crop for sugar production, faces significant yield losses caused by the black bean aphid Aphis fabae (Scop.) and the green peach aphid Myzus persicae (Sulzer), which also transmits viruses. The restriction on neonicotinoid usage in Europe has intensified this problem, emphasizing the urgent need for breeding resistant crop varieties. This study evaluated 26 sugar beet germplasms for resistance against both aphid species by using performance and feeding behavior assays. Additionally, whole plant bioassays and semi-field experiments were carried out with Myzus persicae. Our findings demonstrate the presence of temporal resistance against both aphid species in the primary sugar beet gene pool. Beet yellows virus (BYV) carrying aphids showed enhanced performance. Different levels of plant defense mechanisms were involved including resistance against Myzus persicae before reaching the phloem, particularly in sugar beet line G3. In contrast, resistance against Aphis fabae turned out to be predominately phloem-located. Furthermore, a high incidence of black inclusion bodies inside the stomach of Myzus persicae was observed for approximately 85% of the plant genotypes tested, indicating a general and strong incompatibility between sugar beet and Myzus persicae in an initial phase of interaction. Sugar beet resistance against aphids involved different mechanisms and is species-specific. The identification of these mechanisms and interactions represents a crucial milestone in advancing the breeding of sugar beet varieties with improved resistance. © 2023 Julius Kühn-Institut and The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Bio-Insecticidal Efficacy of Aegle Marmelos Against Myzus Persicae

Myzus persicae (Green peach aphids) has wide range of distribution in different plant hosts. As a pest it causes adverse effect to crops both directly and indirectly by transmitting different viruses and also by producing honey dew. Myzus persicae transmits almost 100 different viral diseases of plants on thirty different plant families which include some major crops like, sugar cane, tobacco, paddy, citrus, sugar beet etc. Myzus persicae is cosmopolitan in distribution. This insect pest causes damage to wide range of economically important crops and their control is usually dependent upon chemicals, intensive use of which may develop resistance in them. During this experiment botanical pesticide is used as natural control measure and tested its efficacy against Green peach aphids at different concentrations. The plant used is Aegle marmelos. Plant parts such as stem and leaves were used to prepare Boiling and Soxhlet extract. Each extract of plant was individually tested against the aphids’ nymph which gave maximum mortality of about 97.7% results at the concentration of 500 ppm. The use of this botanical pesticide helps to eradicate the crop pests by an eco-friendly manner.

Effect of Rickettsiella viridis endosymbionts introduced into Myzus persicae aphids on parasitism by Diaeretiella rapae: A combined strategy for aphid control?

Aphids are major crop pests in southeastern regions of Australia. Some aphid species harbor heritable facultative endosymbionts that may induce beneficial or detrimental impacts on aphids under certain ecological conditions. Aphid-parasitoid interactions can be greatly affected by facultative endosymbionts but there is still limited research on many species of economic significance. Here we assessed the effects of a facultative endosymbiont, Ricketsiella viridis, on parasitism of the major aphid pest, Myzus persicae, by Diaeretiella rapae. We found that R. viridis does not provide M. persicae with significant protection against D. rapae, with parasitoids showing a preference for probing aphids infected with R. viridis. The fecundity of M. persicae is reduced due to infection with R. viridis regardless of the presence of parasitoids. Moreover, we show that parasitoids may facilitate horizontal and subsequent vertical transmission of facultative endosymbionts in aphids which could increase the spread of deleterious effects associated with R. viridis. Based on these findings, simultaneous release of D. rapae and M. persicae infected with R. viridis in the early cropping season (lower population densities and cooler conditions) may contribute to an effective strategy for efficient management of this pest.

Optimizing ethyl formate fumigation in greenhouse cucurbit crops for efficient control of major agricultural pests, Myzus persicae and Thrips palmi

BackgroundEthyl formate (EF) is naturally occurring volatile compound used as quarantine fumigant for pest control. Recently, conversion of uses of EF was tried from quarantine to agricultural field due to its promising efficacy. However, there is a lack of studies on the residue pattern on crops and soil and the phytotoxic mechanism of EF in greenhouse environment. This study aimed to evaluate the efficacy, residue analysis, and phytotoxicity of EF fumigation in controlling Myzus persicae and Thrips palmi, on cucurbit crops and establish an optimized fumigation strategy for use in greenhouses.ResultsThe results showed that EF was more effective against M. persicae than against T. palmi. Residue analysis indicated that EF rapidly decomposed and was not retained after 30 min in leaves and 2 h in soil after fumigation, suggesting the potential for residue-free pest control. Phytotoxicity test revealed that watermelon was the most sensitive crop to EF, and H2O2 accumulation was observed above a concentration of 7.5 g/m3. A strategy to reduce phytotoxicity with sodium bicarbonate during fumigation showed promising results in reducing phytotoxic effects on the crops. The optimized EF fumigation with 6 g/m3 was applied in a greenhouse, resulting in 100% and 40% mortality of M. persicae and T. palmi, respectively, with no notable phytotoxicity and EF residue in the treated crops and soil.ConclusionThis study demonstrates that optimized EF fumigation can be an environmentally sustainable method for controlling pests in greenhouses, paving the way for improved pest management practices and sustainable agriculture. Further research is needed to validate these findings and explore the potential of EF fumigation for other crops and pests.Graphical

Viruliferous and non‐viruliferous aphids behave differently to enhance the spread of the virus

AbstractPlant viruses can modulate insect vector behaviour directly and/or indirectly by altering plant biochemistry. In many instances, such behavioural alterations increase the frequency of encounters between host and vector, leading to enhanced virus dispersal in ecosystems. In the current study using Cucumber mosaic virus (CMV), Capsicum annuum L. (Solanaceae), and Myzus persicae (Sulzer) (Hemiptera: Aphididae) as a pathosystem, we evaluated the direct and indirect effects of CMV on M. persicae biology and behavioural responses. Data showed that CMV indirectly modulates the aphid vector by providing fitness benefits to aphids, leading to enhanced aphid reproduction on CMV‐infected pepper plants in comparison with non‐infected C. annuum plants. Furthermore, CMV can also alter aphid behaviour directly by modulating the behavioural response of viruliferous aphids towards non‐infected plants. We demonstrate that CMV directly and indirectly modulates the insect vector thereby enhancing its transmission in agroecosystems.

Fusion dsRNA in targeting salivary protein genes enhance the RNAi-based aphid control

RNA interference (RNAi) is a promising tool for pest control and relies on sequence-specific gene silencing. Salivary proteins are cooperatively secreted into plants to guarantee the feeding of aphids; thus they have potential to develop as selective targets for RNAi-based pest control strategy. For this purpose, we firstly analyzed 18 salivary proteomes of various aphid species, and these salivary proteins can be mainly categorized into seven functional groups. Secondly, we created a work-flow for fusion dsRNA design that can target multiple genes but were selectively safe to beneficial insects. Based on this approach, seven fusion dsRNAs were designed to feed the green peach aphid, which induced a significant reduction in aphid fitness. Among them, ingestion of dsperoxidase induced the highest mortality in aphids, which was also significantly higher than that of traditional dsRNAs in targeting three peroxidases separately. In addition, dsperoxidase-fed green peach aphids triggered the highest H2O2 content of host plants as well as the attraction to natural enemies (ladybeetle and parasitic wasp) but repellent to other control aphids. Our results indicate that the fusion dsRNA design approach can improve aphid control capacity, and the fusion dsRNA targeting salivary protein-encoding genes can enhance the direct and indirect defenses of host plants, thus providing a new strategy for RNAi-based aphid control.

Identification of Pests and Diseases in Red Chili Plants (Capsicum Annuum L.) in Nangsri, Manisrenggo, Klaten

Red chilies are one of the important types of vegetables cultivated commercially in tropical countries. Insect pests are one of the obstacles in efforts to increase the production of red chili ornamental plants. There are some farmers who do not understand the types of pests that exist in red chili plantations. This research aims to identify the types of pests that attack red chili plants in Nangsri Manisrenggo Village and observe the symptoms of pest and disease attacks. The methods used are observation and interviews. The data obtained was analyzed descriptively qualitatively. The results of the research show that there are 2 types of disease, namely fungi that cause leaf spots, Pestalotia sp and Cercospora sp, and 6 types of pests that attack red chilies, namely: Thrips spp., peach aphids (Myzus persicae), armyworms (Spodoptera litura), flies fruit (Bactrocera spp.), mites (Tetranychus sp.; Polyphagotarsonemus sp.) and whitefly (Bemisia tabaci).

Terpene produced by coexpression of the TPS and P450 genes from Lavandula angustifolia protects plants from herbivore attacks during budding stages

To deter herbivore attacks, plants employ a diverse array of volatile compounds, particularly during the early developmental stages. The highly expressed genes LaTPS7, LaTPS8, and LaCYP71D582 were identified during the budding phases of Lavandula angustifolia. In vitro studies revealed that LaTPS7 generated nine distinct compounds, including camphene, myrcene, and limonene. LaTPS8 enzymatically converted eight volatiles by utilizing geranyl diphosphate and nerolidyl diphosphate as substrates. Overexpression of plastid-localized LaTPS7 in Nicotiana benthamiana resulted in the production of limonene. Furthermore, the endoplasmic reticulum-associated enzyme LaCYP71D582 potentially converted limonene into carveol. In N. benthamiana, LaTPS8 is responsible for the synthesis of α-pinene and sylvestrene. Furthermore, leaves transfected with LaTPS7 and leaves cotransfected with LaTPS7 and LaCYP71D582 exhibited a repellent effect on aphids, with an approximate rate of 70%. In comparison, leaves with an empty vector displayed a repellent rate of approximately 20%. Conversely, tobacco leaves expressing LaTPS7 attracted ladybugs at a rate of 48.33%, while leaves coexpressing LaTPS7 and LaCYP71D582 attracted ladybugs at a slightly higher rate of 58.33%. Subsequent authentic standard tests confirmed that limonene and carveol repel Myzus persicae while attracting Harmonia axyridis. The promoter activity of LaTPS7 and LaCYP71D582 was evaluated in Arabidopsis thaliana using GUS staining, and it was observed that wounding stimulated the expression of LaTPS7. The volatile compounds produced by LaTPS7, LaTPS8, and LaCYP71D582 play a crucial role in plant defence mechanisms. In practical applications, employing biological control measures based on plant-based approaches can promote human and environmental health.