Green Character Research Articles

Application of zwitterionic ionic liquid-based capsule phase microextraction for the HPLC-UV determination of doxycycline in human urine samples

Herein, we describe the utilization of an ionic liquid (IL)/Carbowax 20 M−functionalized sol–gel sorbent for the capsule phase microextraction of doxycycline in authentic human urine samples. This green sample preparation method combines stirring and filtration in a single, standalone sample preparation device, streamlining the sample preparation process. Additionally, it provides rapid extraction kinetics and high extraction efficiency. The experimental conditions (i.e. sorbent type, sample pH and volume, extraction time, ionic strength, elution solvent, and volume) affecting the extraction efficiency of the analyte were studied and optimized. The method was linear in the range of 0.1 – 5.0 μg/mL with a coefficient of determination higher than 0.995. The achieved LOD was found to be 0.02 μg/mL while the lower limit of quantitation (LLOQ) was 0.1 μg/mL. The IL/Carbowax 20 M−functionalized microextraction capsules were reusable at least 30 times for urine samples. The relative recoveries (% RR) ranged between 93.4 – 115.9 % while the precision (expressed as % RSD) was better than 8.1 % in all cases. The robustness of the microextraction procedure and the instrumental HPLC method were separately investigated using Plackett-Burman experimental designs. The analytical protocol demonstrated cost-effectiveness, ease of handling, and speed, leading to increased sample throughput. The green character of the developed method was evaluated using the Green Analytical Procedure Index (GAPI) and Blue Applicability Grade Index (BAGI). Finally, the method’s applicability was demonstrated by analyzing authentic human urine samples after oral administration of a doxycycline-containing pharmaceutical formulation.

Resistance of Doubled Haploid Rice Lines with Green Super Rice Characters to Bacterial Leaf Blight

<p>Bacterial leaf blight (BLB), caused by <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (<em>Xoo</em>) is a significant disease attacking rice crops worldwide. This disease attacks at various stages of plant growth and causes significant yield loss. Breeding new varieties resistant to BLB is important to support sustainable agriculture in the future. This study aimed to identify new superior green super rice (GSR) lines resistant to BLB disease. The experiment evaluated the resistance of lowland rice lines obtained from anther culture using a factorial randomized complete block design. The 1<sup>st</sup> factor was genotype, consisting of 20 lines, 2 checks of commercial varieties (Inpari 42 Agritan GSR and Inpari 18), a resistant check (Conde), and a susceptible check (Taichung Native 1). The 2<sup>nd</sup> factor was BLB pathotypes (i.e., III, IV, and VIII). Quantitative data on disease severity and severity index were analyzed using analysis of variance and t-Dunnett test at 5% level. The results showed that the interaction between genotype and pathotype affected the disease severity and severity index in both growth phases. The tested lines exhibited varying resistance, from susceptible to resistant, to BLB. Four lines (SN 11, 13, 57, and 58) showed moderate to resistant criteria for BLB disease of 3 pathotypes in both growth phases. The selected lines can be used as a source of parents for breeders and candidates for new superior varieties with BLB resistance properties to support the reduction of synthetic chemical bactericide inputs and control BLB disease. However, further field evaluations are necessary to assess their performance.</p>

Anidación de aves y mamíferos arborícolas en el campus de la Universidad del Mar, Puerto Escondido, Oaxaca

Some faunistic species require certain characteristics of vegetation for the preparation of their nests and depending on their availabi- lity, nesting will occur more or less frequently. Some sites such as the Puerto Escondido campus of the Universidad del Mar (UMAR) represent an urban space of special interest because it maintains a green island character as surrounded by urban expansion offering potential for nesting fauna species. The aim was to learn about the nesting of birds and arboreal mammals present at the UMAR Puerto Escondido campus. From March to June 2024, we conducted systematic weekly walks in search of evidence of nests or the beginning of its construction. For each nest we recorded its location by using a GPS and the height in which they were observed, as well as the tree where they were built. A total of 104 nests were registered, 55 nests (52.8%) belonged to Sciurus aureogaster and the nests of Columbina inca and Pitangus sulphuratus were registered at a lower frequency. 60.3% (n=58) of the nests were recorded in heights below 10 m, 28% (n=27) were recorded in heights between 11 and 15 m, and the rest (11.7%, n=19) were found in heights higher than 16 m. Acacia cornigera was the tree species that presented the largest number of nests (n=21) recorded. The information resented in this work brings to the knowledge of the use of resources by faunastic species of interest, mainly because of the contribution to nesting behavior in anthropized environments.

Μagnet integrated fabric phase sorptive extraction (MI-FPSE) for the selective isolation of seven sulfonamides from human urine prior to HPLC-DAD analysis

In the present study magnet integrated fabric phase sorptive extraction (MI-FPSE) was employed for the selective extraction of seven sulfonamides (i.e., sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfamethoxazole, sulfisoxazole and sulfadimethoxine) from human urine prior to their determination by high pressure liquid chromatography – diode array detection (HPLC-DAD). The MI-FPSE protocol was optimized after studying the critical parameters that affect extraction, namely: type of sol–gel sorbent, extraction time, desorption time, stirring rate, type and volume of elution solvent, the ionic strength and the pH of the sample matrix using the one-factor-at-a-time method. The developed MI-FPSE-HPLC-DAD method was validated in terms of linearity, sensitivity, selectivity, accuracy, and precision and presented satisfactory results. The limits of detection (LODs) and quantification (LOQs) ranged between 0.02–0.04 ng/μL and 0.06–0.15 ng/μL, respectively. The RSD% values of the intra-day and inter-day assays were found lower than 6.7 % and 9.4 %, respectively, showing good precision. Accuracy was assessed using percentage of relative recovery and varied from 86.3–112.9 % (intra-day study) and 85.5–106.9 % (inter-day study) for all examined analytes. The green character and practicality of the proposed method were investigated using the ComplexGAPI index and Blue Applicability Grade Index (BAGI).

Energy-safety balanced composites of attractive cyclic nitramines with polyaniline

Composite microcrystals of the cyclic nitramines 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (ε-CL-20), 1,3,5-trinitro-1,3,5-triazinane(RDX), 1,3,5,7-tetranitro-1,3,5,7-tetrazocane (β-HMX), and cis-1,3,4,6-tetranitrooctahydroimidazo-[4,5-d]imidazole (BCHMX) with polyaniline (PANi) have been prepared and thoroughly characterized in terms of morphology and phase purity. PANi outperformed other conducting polymers in terms of selectivity towards NAs due to its better interaction with NAs, low production cost, and ease of preparation. The bonding of nitramines with the polymeric PANi chain has been examined by means of Fourier transform infrared (FTIR), Raman spectroscopy methods, and fluorescence quenching; the Raman spectrum has shown the laser sensitivity of these microcrystals. Powder X-ray diffraction results have shown changes in polymorph modifications in CL20 (from ɛ to β) and HMX (from β to α) during the preparation of the composites, which have also been confirmed by spectral and differential thermal analysis techniques. The structural orientations found in these composites significantly stabilize nitramines against impact; their detonation properties have slightly deteriorated, but the PANi electrical conductivity has strongly increased their electric-spark sensitivity. The above properties of the prepared composites determine their potential use mainly as parts of the electric or laser impulse initiators, having “a green character”, for various charges.

Implementation of green-assessed nanotechnology and quality by design approach for development of optical sensor for determination of tobramycin in ophthalmic formulations and spiked human plasma

A fast eco-friendly colorimetric method was developed for the determination of Tobramycin in drug substance, ophthalmic formulations, and spiked human plasma using silver nanoparticles optical sensor. Even though tobramycin is non-UV–visible absorbing, the developed method is based on measuring the absorbance quenching of silver nanoparticles resulting from the interaction with tobramycin. Different factors affecting the absorbance intensity were studied as; silver nanoparticle concentration, pH, buffer type, and reaction time using quality by design approach. Validation of the proposed method was performed according to ICH guidelines and was found to be accurate, precise, and sensitive. The linearity range of tobramycin was 0.35–4.0 μg/mL. The optical sensor was successfully applied for the determination of Tobramycin in ophthalmic formulations and spiked human plasma without pre-treatment. Additionally, the binding between Tobramycin and PVP- capped silver nanoparticles was studied using molecular docking software. The method was assessed and compared to colorimetric reported methods for the green character using Green Analytical Procedure Index (GAPI) and Analytical GREEnness calculator (AGREE) tools and found to be greener.

Efficient Demulsification of Crude Oil Emulsion Using Novel Sugar-based Surfactant.

This study aimed to synthesize ecofriendly and low-cost surfactant-based sugar, HA-ST, under mild conditions and a short route via an opening ring of hexadecylsuccinic anhydride (HA) using starch (ST). HA-ST's chemical structure, thermal behavior, and surface activity were evaluated using Fourier Transform Infrared (FTIR) spectroscopy, thermogravimetric analysis, and a pendant drop technique. The results indicated HA-ST formation, thermal stability, and surface activity. HA-ST's green character, low cost, and surface activity recommended its use as a demulsifier for crude oil emulsions at different affecting parameters such as temperature, seawater ratio (SR), demulsifier concentration, demulsification time (DT), and pH. HA-ST demulsification efficiency (DE) was evaluated and compared with a commercial demulsifier (CD). The results showed improved HA-ST's DE with rising temperature, SR, demulsifier concentration, DT, and pH. The DE of HAST reached 100% at 50% of SR and 250 ppm of demulsifier concentration; the same results were obtained using CD. In contrast, HA-ST gave relatively lower DE at low SR (10%) with a value of 70% than the obtained using CD with a value of 75%. The green character, low cost, and DE of HA-ST make it suitable for demulsifying crude oil emulsions, especially those containing more than 30% seawater, compared with CD, which commonly contains two or more traditional surfactants.

A green liquid phase microextraction using phthalic acid as switchable hydrophilicity solvent for the HPLC determination of sildenafil (Viagra®) in human urine

Herein, a green liquid phase microextraction protocol using phthalic acid as switchable hydrophilicity solvent (SHS) is reported for the quantification of sildenafil in authentic human urine. The analyte was extracted onto phthalic acid solid particles which were produced through acidification of the sample. Its solidification was accomplished at ambient conditions without sample cooling. The determination of the sildenafil was carried out using high performance liquid chromatography-ultraviolet detection (HPLC-UV). The microextraction parameters that affect the extraction efficiency of the drug (i.e. SHS type and its concentration, acid type and concentration, extraction time, filter type) have been studied. The optimized analytical protocol involved the mixing of 300 μL of phthalate solution (0.75 M) with 600 μL of sample, followed by the addition of 50 μL of concentrated H3PO4. The produced solid was collected using membrane syringe filter (0.45 μm) and was finally dissolved in 500 μL of CH3OH. Method validation data showed determination coefficient ≥ 0.99 for the linear range of 50 – 2000 ng/mL. The limit of detection (LOD) and the lower limit of quantitation (LLOQ) were 30 and 100 ng/mL, respectively. The accuracy (expressed as % recovery) of the method ranged between 88.0 – 108.9 % while the precision (expressed as % RSD) was less than 17.8 % in all cases. The robustness of the microextraction procedure and the instrumental method were investigated using Plackett-Burman experimental designs. The applicability of the method was demonstrated by analyzing both spiked and authentic human urine samples after oral administration of drug-containing pharmaceutical formulation. The developed protocol offers cost-efficiency, handling simplicity, and high throughput. Its green character was evaluated using Green Analytical Procedure Index and Blue Applicability Grade Index.

Ionic-liquid/Carbowax 20 M functionalized capsule phase microextraction platform for the extraction of phosphodiesterase-5 inhibitors from human serum and urine prior to their determination by LC–MS

Capsule phase microextraction (CPME) is an efficient bioanalytical technique that streamlines the sample preparation by integrating the filtration and stirring mechanism directly into the device. A novel composite sorbent designed to be selective towards the target analytes consisting of mixed-mode sorbent chemistry synthesized by sol–gel technology is found promising and superior to the conventional C18 sorbents. Herein we describe the encapsulation of an ionic liquid (IL)/Carbowax 20M-functionalized sol–gel sorbent (sol–gel IL/Carbowax 20 M) in the lumen of porous polypropylene tubes for the capsule phase microextraction of three phosphodiesterase-5 inhibitors namely avanafil, sildenafil, and tadalafil in human serum and urine samples. The CPME device was characterized by Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FT-IR). The experimental parameters of CPME procedure (e.g. sample pH and ionic strength, extraction time, stirring rate, elution solvent and volume) were carefully optimized to achieve the highest possible extraction efficiency for the analytes. Method validation was conducted in terms of precision, linearity, accuracy, matrix effect, lower limits of quantification, and limits of detection (LOD). The method linearity was investigated in the range of 50–1000 ng mL−1 for all analytes while the precision was less than 11.8 % in all cases. For all analytes, the LOD values were 17 ng mL−1. The IL/CW 20M-functionalized microextraction capsules could be reused at least 25 times both for urine and serum samples. The green character and the applicability of the proposed method were evaluated using the ComplexGAPI and BAGI indexes. The optimized CPME protocol exhibited reduced consumption of organic solvent and generation of waste, cost-effectiveness, and simplicity. Finally, the proposed method was successfully applied to the analysis of sildenafil in human urine after administration of drug-containing formulation.

Quality by design−aided acid-free synthesis of self P, N, S-doped black seed-derived carbon quantum dots for application as a nanosensor for eltrombopag environmental and bioanalysis and pharmacokinetic assay

Herein, we developed a rapid, one-step, and cost-effective methodology based on the fabrication of water-soluble self-nitrogen, sulfur, and phosphorus co-doped black seed carbon quantum dots (BSQDs) via microwaveirradiation in six minutes. Our synthesis approach is superior to those in the literature as they involved long-time heating (12 h) with sulfuric acid and sodium hydroxide and/or high temperatures (200 °C). A full factorial design was applied to obtain the most efficient synthesis conditions.BSQDs displayed excitation-independent emissions, demonstrating the purity of the synthesized BSQDs, with a maximum fluorescence at 425 nm after excitation at 310 nm. Eltrombopag olamine is an anti-thrombocytopenia drug that is also reported to cause toxicity in river water based on its Persistence, Bioaccumulation, and Toxicity (PBT). The synthesized BSQDs were employed as the first fluorometric sensor for environmental and bioanalysis of eltrombopag. The fluorescence of BSQDs decreased with increasing concentrations of eltrombopag, with excellent selectivity and sensitivity down to 30 ppb. BSQDs were successfully applied as sensing probes for the detection of eltrombopag in medical tablets, spiked and real human plasma samples, and river water samples, with an overall recovery of at least 97 %. The good tolerance to high levels of foreign components and co-administered drugs indicates good selectivity and versatility of the proposed methodology. Plasma pharmacokinetic parameters such as t1/2, Cmax, and t max of eltrombopag were evaluated to be 9.91 h, 16.0 μg mL−1, and 5 h, respectively. Moreover, the green character of the BSQDs as a sensor was proved by various analytical greenness scales.

Gene effect analysis of forage quality traits in barley (Hordeum vulgare) in Bundelkhand region of India

Present study was carried out during winter (rabi) seasons of 2019–20 and 2020–21 at Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh to decipher underlying gene effects for green forage quality characters in barley (Hordeum vulgare L.) in Bundelkhand region of Uttar Pradesh. Eight diverse barley genotypes, viz. BH902, BH946, DWRB160, DWRB180, RD2552, RD2794, RD2899 and RD2907 were evaluated and crossed in half diallel design. A total of 28 F1s along with 8 parents were sown in randomized complete block design (RCBD) with 3 replications. The green fodder was harvested after 55 days of sowing (DAS) and fodder quality analysis was conducted. The crude protein (CP) and nitrogen (N) contents showed mean values of 14.19 and 2.49% ranged from 12.62–15.26% and 2.22–2.68%, respectively. The characters, viz. organic matter (OM), CP, N, lignin (L) and hemicellulose (HCL) showed prepondrance of additive gene effects, whereas acid detergent fibre (ADF), neutral detergent fibre (NDF) and cellulose (CL) showed the prevalnce of dominance gene effects. The genotype DWRB180 and two rowed malt barley variety DWRB160 were estimated with desirable additive gene effects for forage intake and digestibility traits. The cross combinations, DWRB160/RD2899, BH946/RD2552 and RD2794/DWRB180 can be further utilized for CP and N contents, whereas, the progenies, viz. BH946/DWRB180 and RD2552/RD2907 were promising for ADF and NDF. GT biplot corroborated positive correlations of NDF with ADF (0.43**), HCL (0.88**) and CL (0.41*). The improvement in forage intake and digestibility characters like ADF, NDF and CL is suggested through complex crossing followed by selection.

A green flow-batch liquid phase microextraction lab-in-syringe platform based on natural deep eutectic solvents for toxic metal determination

In this work, a novel functional and reliable computer-controlled flow-batch lab-in-syringe (LIS) platform was developed for liquid phase microextraction (LPME) based on the use of natural deep eutectic solvents (NADES) as green alternatives to conventional extractants. The proposed automatic on-line LIS system is reported for the first time and demonstrated for the determination of toxic metals in aqueous samples, as a front-end to flame atomic absorption spectrometry (FAAS). To the best of our knowledge, the determination of metals by atomic spectrometric techniques processing a LIS preconcentration system using NADES has not been previously reported. The analytical process included the in-syringe metal complexation of Cd, Cu, and Pb (model analytes) with ammonium pyrrolidine dithiocarbamate and the subsequent microextraction of the resulted complexes by a mixture of menthol:heptanoic acid at a molar ratio of 1:2. After phase separation, the NADES was transferred towards the flame for direct atomization in an automatic and precise manner. The main attributes that affect the performance of the method were thoroughly studied. Under optimum conditions, the limits of detection ranged between 0.54 and 4.52 μg L−1 corresponding to a sample consumption of 4000 μL. The relative standard deviations were less than 3.8% demonstrating good method precision. As a proof-of-concept, the developed system was used for the analysis of drinking and environmental water samples resulting in good relative recoveries (i.e., 94.0–104.0%). The green character and the applicability of the proposed approach were demonstrated by the Green Analytical Procedure Index (GAPI), and Blue Applicability Grade Index (BAGI), respectively.

Micro-solid phase extraction of pharmaceuticals and illicit drugs from wastewaters using monolith-coated syringe filters

In this study, a porous monolith of poly(methacrylic acid-co-ethylene glycol dimethacrylate) has been prepared on the nylon membrane of a commercial syringe filters (13 mm, 0.22 µm) for the micro solid-phase extraction (µSPE) of pharmaceutical and illicit drugs from wastewater samples prior to liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination. Polymer synthesis conditions were designed and adjusted to provide robust devices with reduced costs and ease of use. The performance of the developed devices was assessed by investigating the most appropriate µSPE conditions. The features of the proposed device allow for the extraction of sample volumes up to 25 mL of wastewater and the use of minimal amounts of organic solvents (200 µL of 0.1 % formic acid in methanol) in the desorption step, providing a 125 enrichment factor. Sample extracts were measured by LC-MS/MS, providing limits of detection from 2 to 22 ng/L, recoveries from 84 to 120 %, and relative standard deviations lower than 16 %. The proposed approach has demonstrated to be suitable for the easy and simple extraction of forty-two psychoactive substances from wastewater samples with a reduced solvent consumption, low cost, minimal environmental impact, and high enrichment capacity. The method greenness and practicality has been evaluated using AGREEprep, complexGAPI, and BAGI tools, demonstrating an adequate green character and practicality of the proposed methodology.

Deep eutectic solvents applications aligned to 2030 United Nations Agenda for Sustainable Development

Sustainable living means understanding how lifestyle choices impact the world and how the livelihoods of current and future generations can be sustained. If the current production and processing patterns are switched to sustainable ones, this will contribute to diminishing adverse effects such as pollution and climate change. Deep eutectic solvents (DESs) are biodegradable, biocompatible, low cost and tunable green solvents extensively analyzed in different areas to replace traditional harsh organic solvents. DESs are rapidly emerging in numerous applications owing to their green character and physicochemical properties, leading to their used, not only as solvent systems, but as catalysts, adsorbent agents, electrolytes or nanocarriers for the encapsulation of anticancer drugs, and in very diverse areas. Therefore, DESs can contribute to developing sustainable processes from different points of view. This review presents the main applications of DESs aligned to the United Nations 2030 Agenda for Sustainable Development, specifically to those related to energy efficiency improvement, sustainable production patterns and action to combat climate change. Giving an overview about the implementation of DESs for process intensification and sustainable production, and in some cases how authors reconfigured processes using DESs to obtain cost effective, less labor-intensive, ecofriendly, safe, and highly effective approaches.

Utilization of a pH-switchable hydrophilicity solvent for the microextraction of clomipramine from human urine samples

Clomipramine (CLP) is a tricyclic antidepressant drug, and its determination in biological samples is of high importance in clinical and forensic evaluations to assure appropriate drug concentrations. In the present study, benzoic acid was employed as a pH-switchable hydrophilicity solvent (SHS) for the microextraction of CLP from authentic human urine samples prior to its determination by high performance liquid chromatography-ultraviolet detection (HPLC-UV). The microextraction protocol was based on the phase transition of the SHS through pH alteration that resulted in its rapid dispersion and simultaneous phase separation. The obtained solid was collected in a syringe filter, dissolved in methanol, and analyzed. The main parameters that affected the efficiency of the microextraction procedure were studied and optimized to ensure high extraction efficiency for CLP and the analytical method was validated. Under optimum conditions, good linearity was observed between 0.05 and 5.0 μg mL−1. The limit of detection and limit of quantification were found to be 0.015 and 0.05 μg mL−1, respectively. The RSD values for intra-day repeatability and inter-day precision were 2.4–8.9 % and 1.7–9.1 %, respectively. The relative recovery values were within 90.0 and 110.0 % in all cases, demonstrating good method accuracy. The proposed SHS microextraction showed cost-efficiency, handling simplicity, and rapidity resulting in enhanced sample throughput. Moreover, the proposed method exhibited a green character and good applicability based on its evaluation by Green Analytical Procedure Index and Blue Applicability Grade Index.

Characterization of Coloured Sorghum Genotypes for Qualitative and Quantitative Characters

The experiment was conducted at College of Agriculture, Raichur, University of Agricultural Sciences, Raichur, Karnataka, India during October 2021–Februrary 2022 to characterize the 100-coloured sorghum genotypes along with four checks viz., M 35-1, AKJ 1, Paiyur 2 and GS-23 for eight qualitative characters and 10 quantitative characters in an augmented block design. The results revealed that, majority of the genotypes exhibited the pigmented stem (93%) and leaves almost green (61%) at harvest, horizontal flag leaf orientation (100%), loose erect primary branches of panicle (38%), black glumes (58%), red grain colour (36%), circular grain shape (75%) and freely threshable (93%) characters. For all the quantitative characters studied, a wide range of variation was observed. For stay green character, genotype IS 21868 showed completely green leaves. Similarly, for other characters also superior genotypes were identified in this study. Such genotypes can be used further in improvement of sorghum for its grain yield. For grain yield, 14 genotypes exhibited higher yields when compared to the best check GS-23, viz., IS 29032 (120.0 g), IS 6508 (119.18 g), IS 16006 (113.80 g), IS 28065 (102.88 g), IS 23890 (94.88 g), IS 28049 (94.28 g), IS 23865 (89.48 g), IS 29031 (87.98), IS 28202 (87.88), IS 31706 (86.48), IS 2582 (85.88 g), IS 28200 (83.58), IS 16398 (81.08 g) and IS 23955 (80.0 g). Eight genotypes were on par with GS-23. These genotypes can be further evaluated for its yield stability within and across locations.

Basic Fuchsin Dye as the First Fluorophore for Optical Sensing of Morpholine in Fruits Crust and Urine Samples.

In this work, basic fuchsin (BF) dyestuff was presented as the first optical sensor used for the spectrofluorimetric assessment of morpholine (MOR) where BF exhibits morpholine-sensing behavior. The developed fluorimetric avenue is sensitive, facile, selective, and validated for assaying the sensitizing influence of MOR on the BF fluorescence in an aprotic dioxane solvent. Parameters like solvents, BF concentration, order, and time of addition that influence the fluorescence intensity of the probing system were addressed. Optimizing the analytical methodology revealed a linear fluorescence sensitization within the addition of MOR in the two concentration ranges of 5 × 10-9 to 1.0 × 10-7 mol L-1 and 2.0 × 10-7 to 3 × 10-6 mol L-1. The limit of detection (LOD) and limit of quantification (LOQ) were estimated to be 2.0 × 10-9 mol L-1 (0.17 ng mL-1) and 6.66 × 10-9 mol L-1 (0.567 ng mL-1), respectively. High levels of accuracy and precision are achieved when assaying spiked MOR either in pure solutions or samples of fruit peel extract and human urine. Moreover, the green character and practicality/applicability of the method were evaluated by AGREE and BAGI metric tools. These merit outcomes provide insights into the development of fluorescent sensors for MOR detection using fluorescent dyes and meet the Food and Drug Administration's requirements for morpholine detection in real-life applications.

A magnetic stir bar sorbent of metal organic frameworks, carbon foam decorated zinc oxide and cryogel to enrich and extract parabens and bisphenols from food samples

A porous composite magnetic stir bar adsorbent was fabricated for the extraction and enrichment of parabens and bisphenols from selected beverage samples. The adsorbent comprised a metal organic framework, carbon foam decorated zinc oxide and magnetic nanoparticles embedded in polyvinyl alcohol cryogel. The porous composite stir bar adsorbent could adsorb parabens and bisphenols via hydrogen bonding, π-π and hydrophobic interactions. In the best conditions, linearity was good from 5.0 to 200.0 µg/L for methyl paraben, ethyl paraben and bisphenol A and from 10.0 to 200.0 µg/L for bisphenol B and butyl paraben. Limits of detection ranged from 1.5 to 3.0 µg/L. The developed composite stir bar was successfully applied to extract and determine parabens and bisphenols in fruit juice, beer and milk. Recoveries ranged from 89.5 to 99.5 % with RSDs lower than 6 %. The developed sorbent and new methodology were evaluated in terms of its green character with satisfactory results.

Controllably Self-Assembled Antibacterial Nanofibrils Based on Insect Cuticle Protein for Infectious Wound Healing.

Developing self-assembled biomedical materials based on insect proteins is highly desirable due to their advantages of green, rich, and sustainable characters as well as excellent biocompatibility, which has been rarely explored. Herein, salt-induced controllable self-assembly, antibacterial performance, and infectious wound healing performance of an insect cuticle protein (OfCPH-2) originating from the Ostrinia furnacalis larva head capsule are investigated. Interestingly, the addition of salts could trigger the formation of beaded nanofibrils with uniform diameter, whose length highly depends on the salt concentration. Surprisingly, the OfCPH-2 nanofibrils not only could form functional films with broad-spectrum antibacterial abilities but also could promote infectious wound healing. More importantly, a possible wound healing mechanism was proposed, and it is the strong abilities of OfCPH-2 nanofibrils in promoting vascular formation and antibacterial activity that facilitate the process of infectious wound healing. Our exciting findings put forward instructive thoughts for developing innovative bioinspired materials based on insect proteins for wound healing and related biomedical fields.

Eco-friendly design of experiment based approach for estimation of azelnidipine and olmesartan medoxomil in RP-HPLC: Greenness appraisal

The analytical chemistry community has prioritized the implementation of green analytical methods in recent years to minimize the adverse effect on the environment and human beings. The combination of Azelnidipine (AZD) and Olmesartan medoxomil (OLM) was used to manage hypertension. Thus, the present study aimed to develop an RP-HPLC technique to determine AZD and OLM simultaneously by the combined framework of design of experiments and green analytical principles. A central composite design (CCD) was employed for method optimization, with ethanol, and flow rate was selected as critical variables. The effective separation was performed by Agilent Eclipse Plus (C18, 250 × 4.6 mm i.d, 5 μm) with a mobile phase comprising ethanol and 1% v/v aqueous acetic acid in the ratio of 49.5:50.5 I flowing at 1 mL/min, the detection wavelength at 250 nm. The retention time for AZD and OLM were 6.362 and 3.323, respectively. Linearity was perceived over the concentration ranges of 6.4–9.6 and 16–24 μg/mL for AZD and OLM, respectively. The green character of the proposed method showed excellent green results. This innovative eco-friendly, design of experiment technology was adequate for regular analysis of AZD and OLM in pharmaceutical dosage form without harming the environment.