Eco-friendly design of experiment based approach for estimation of azelnidipine and olmesartan medoxomil in RP-HPLC: Greenness appraisal

Abstract

The analytical chemistry community has prioritized the implementation of green analytical methods in recent years to minimize the adverse effect on the environment and human beings. The combination of Azelnidipine (AZD) and Olmesartan medoxomil (OLM) was used to manage hypertension. Thus, the present study aimed to develop an RP-HPLC technique to determine AZD and OLM simultaneously by the combined framework of design of experiments and green analytical principles. A central composite design (CCD) was employed for method optimization, with ethanol, and flow rate was selected as critical variables. The effective separation was performed by Agilent Eclipse Plus (C18, 250 × 4.6 mm i.d, 5 μm) with a mobile phase comprising ethanol and 1% v/v aqueous acetic acid in the ratio of 49.5:50.5 I flowing at 1 mL/min, the detection wavelength at 250 nm. The retention time for AZD and OLM were 6.362 and 3.323, respectively. Linearity was perceived over the concentration ranges of 6.4–9.6 and 16–24 μg/mL for AZD and OLM, respectively. The green character of the proposed method showed excellent green results. This innovative eco-friendly, design of experiment technology was adequate for regular analysis of AZD and OLM in pharmaceutical dosage form without harming the environment.