Green Beans Research Articles

Effect of the Drying Method and Storage Conditions on the Quality and Content of Selected Bioactive Compounds of Green Legume Vegetables.

This study aimed to determine the effect of the drying method (freeze-drying, air-drying), storage period (12 months), and storage conditions (2-4 °C, 18-22 °C) applied to two legume species: green beans and green peas. The raw and dried materials were determined for selected physical parameters typical of dried vegetables, contents of bioactive components (vitamin C and E, total chlorophyll, total carotenoids, β-carotene, and total polyphenols), antioxidative activity against the DPPH radical, and sensory attributes (overall quality and profiles of color, texture, and palatability). Green beans had a significantly higher content of bioactive components compared to peas. Freeze-drying and cold storage conditions facilitated better retention of these compounds, i.e., by 9-39% and 3-11%, respectively. After 12 months of storage, higher retention of bioactive components, except for total chlorophyll, was determined in peas regardless of the drying method, i.e., by 38-75% in the freeze-dried product and 30-77% in the air-dried product, compared to the raw material.

Breeding Strategies for Simultaneous Improvement in Anthracnose Disease Resistance and Economically Important Traits in French Bean

Background: French bean (Phaseolus vulgaris L.) production in the tropics is threatened by heavy incidence of anthracnose disease causing substantial crop loss when infection is at the early growth stages. Breeding strategies for tolerance against anthracnose disease along with enhanced productivity, and high pod protein content are to be formulated. Methods: Genotypes having broad genetic base and phenotypic diversity in 5 bush and 1 pole types were crossed in a 6 × 6 half diallel mating design to estimate combining ability, mode of gene action, and extent of heterosis for 11 quantitative traits. Result: The additive genetic effect was evident for percent disease index (PDI) of anthracnose and 10 pod weight. Rapid genetic gain can be achieved due to predominance of additive gene action in their control and can therefore be selected in early generation through simple breeding methods. Rest of the economic traits controlled by additive and non-additive gene effects could be improved through biparental mating, reciprocal recurrent selection, or diallel selective mating. Anthracnose disease tolerant and high yielding cultivars of might be developed utilizing parents, ‘Laxmi’, ‘Arka Sharath’ and ‘Vaishnavi-264’ with high gca effects. Although two cross combinations ‘Arka Sharath × Lakshmi’ and ‘Arjun × Arka Sharath’ showed significant heterobeltiosis in desired direction for PDI of anthracnose and other desirable horticultural traits but could not be exploited at commercial level due to complexity in hybridization. Identifying pure lines with tolerance against anthracnose disease and favorable horticultural attributes could be accomplished in segregating generations of the prospective hybrids.

The Characteristics of Urease Enzyme of Green Bean Seeds (Vigna radiata L.) and Its Activity as An Antifungal Against Candida albicans

Urease is an enzyme responsible for catalyzing the hydrolysis reaction of urea into CO2 and NH3. The urease isolated in this study came from green bean seeds. The urease enzyme was then tested for its antifungal activity against Candida albicans. The research aims to extract and characterize the urease enzyme from green bean seeds and explore its potential use as an antifungal agent. Green bean seeds were smoothed with a mortar and pestle, followed by homogenization using a stirrer and cold centrifugation. The crude extract of the urease enzyme was assessed for its activity through the Nessler method and measured by employing a UV-Vis spectrophotometer at a wavelength of 500 nm. The well-diffusion method was conducted to determine the antifungal activity of rough enzyme extracts against C. albicans. Positive controls were 100% ketoconazole, and negative controls used a pH buffer of 7. The characterization of the urease enzyme from green bean seeds revealed the optimum urease activity at a concentration of 0.25 M, pH level of 7, and an incubation temperature of 35°C with a value of 32.115 U/mL. Maximum reaction rate (Vmax) and Michaelis-Menten constant value (KM) were obtained at 56.497 U/mL and 0.215 M, respectively. Antifungal tests of C. albicans resulted in strong inhibitory activity at a concentration of 100% crude urease extract of 12 mm inhibition zone. The inhibitory concentration value grows at least 0.5% by 0.25 mm and positive control of 19.802 mm.

Marker assisted transfer of anthracnose resistance gene Co-5 to elite French bean (Phaseolus vulgaris L.) background

Marker assisted transfer of anthracnose resistance gene Co-5 to elite French bean (Phaseolus vulgaris L.) background

Distribution of minerals in the organs of green pea and snap bean plants that could be used in Industry 4.0

Objective: Know the composition of minerals, essential trace elements and toxic trace elements of the organs of pea (Pisum sativum L.) and snap bean (Phaseolus vulgaris) plants for their possible use in industry 4.0.
 Design/methodology/approach: The concentration of mineral elements was determined through inductively coupled plasma mass spectrometry (ICP-MS) and the relationship of the amount of minerals with the flours of the different organs (root, stem, leaves and fruits of peas (P. sativum L.) and green beans (P. vulgaris).
 Results: The leaves represent an important fraction of the dry matter (30-40%) in the plants evaluated and are rich in minerals (calcium, magnesium, phosphorus, potassium), essential trace elements (manganese, iron, selenium, zinc) , toxic trace elements (aluminum, strontium, boron, tin, ba-rium) and rare earth elements (cerium, yttrium, lanthanum and neodymium).
 Limitations on study/implications: The production condition of the crops is unknown, on which the content of mineral elements largely depends.
 Findings/conclusions: The organs of pea and snap bean plants represent an alternative as raw material in the production of foods and biomaterials enriched with minerals, essential trace elements, toxic trace elements and rare earth elements, since these accumulate and biocontain -focus on these organs and these plants can be used as indicators or as phytoextractors to be selected as inputs in industry 4.0 processes.

Metabolite Formation of Pesticide Isoprocarb in Coffee Beans During Short-Term Storage and Condition

Isoprocarb (IPC) is one of the most important carbamate pesticides for white flea control in coffee plants. The prevalence of isoprocarb pesticide residues in coffee, i.e., green coffee beans, is a cause for concern. Green coffee beans were intentionally contaminated with isoprocarb at concentrations >0.01 mg/kg, which was investigated in this research. Quantitative analysis of isoprocarb with the QuEChERS method and qualitative analysis for metabolite formation was characterized using UV-Vis, FTIR, 1H-NMR, GC-MS, and LC-QTOF-MS. Based on the data, the metabolite formed is o-cumenol through the hydrolysis reaction of o-aryl carbamate with the enzyme carboxylesterase. o-cumenol or 2- isopropylphenol as a phenol derivative. To verify the existence of metabolite analytes that are believed to be there in coffee tainted with isoprocarb, more reliable analytical techniques utilizing analytical standards must be developed. Isoprocarb concentrations decreased along the storage time, especially in unsterilized conditions. As a toxic compound, a quantitative structural activity relationship study (QSAR) was initially carried out through a software application for estimating toxicity (TEST) provided by the US Environmental Protection Agency (EPA). For additional information, based on the LC50 and LD50 data confirmed from the TEST application, it was concluded that isoprocarb is more toxic than o-cumenol.

Effect of Gibberellic Acid and Cytokinin on Growth, Quality and Yield of French Bean (Phaseolus vulgaris L.) cv. Ellora No. 11

The experiment was conducted during Rabi season November – March, 2022-2023 at the Vegetable Research Farm, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology & Sciences, and Prayagraj (UP). The experiment was laid out in Randomized Block Design with 10 treatments and 3 replication to study the “Effect of GA3 and Cytokinin on growth, quality and yield of French bean (Phaseolus vulgaris L.) cv. Ellora No 11.” The treatments which areT0: Control, T1:GA3@200ppm, T2: GA3@400ppm, T3:GA3@600ppm, T4:GA3@800ppm, T5:GA3@1000ppm, T6Cytokinin@250ppm, T7Cytokinin@500ppm, T8Cytokinin@750ppm, T9Cytokinin@1000ppm. The growth regulators were sprayed at 10 D.A.S in French bean. from the above experiment finding it is conclude that all the character viz., growth parameter, earliness parameter yield parameter, qualitative parameters varied significantly. Further, The result showed that the minimum days to first flowering(42.33DAS), first picking (63.33DAS) and days to 50% flowering(53.67 DAS) while the maximum plant height(52.07cm), no. of branches(13.57), no. of leaves(214.93), number of pod per plant(21.67),pod length(16.50), pod girth(2.63cm) 1 pod weight(5.70g), yield per ha(12.35t/ha), total soluble solids (6.37°BRIX), protein (1.52%), fiber (3.89%), ascorbic acid (11.77mg)and BC ratio (3.1) was found maximum in T1:GA3@200ppm

Combined sensory, volatilome and transcriptome analyses identify a limonene terpene synthase as a major contributor to the characteristic aroma of a Coffea arabica L. specialty coffee

BackgroundThe fruity aromatic bouquet of coffee has attracted recent interest to differentiate high value market produce as specialty coffee. Although the volatile compounds present in green and roasted coffee beans have been extensively described, no study has yet linked varietal molecular differences to the greater abundance of specific substances and support the aroma specificity of specialty coffees.ResultsThis study compared four Arabica genotypes including one, Geisha Especial, suggested to generate specialty coffee. Formal sensory evaluations of coffee beverages stressed the importance of coffee genotype in aroma perception and that Geisha Especial-made coffee stood out by having fine fruity, and floral, aromas and a more balanced acidity. Comparative SPME–GC–MS analyses of green and roasted bean volatile compounds indicated that those of Geisha Especial differed by having greater amounts of limonene and 3-methylbutanoic acid in agreement with the coffee cup aroma perception. A search for gene ontology differences of ripening beans transcriptomes of the four varieties revealed that they differed by metabolic processes linked to terpene biosynthesis due to the greater gene expression of prenyl-pyrophosphate biosynthetic genes and terpene synthases. Only one terpene synthase (CaTPS10-like) had an expression pattern that paralleled limonene loss during the final stage of berry ripening and limonene content in the studied four varieties beans. Its functional expression in tobacco leaves confirmed its functioning as a limonene synthase.ConclusionsTaken together, these data indicate that coffee variety genotypic specificities may influence ripe berry chemotype and final coffee aroma unicity. For the specialty coffee variety Geisha Especial, greater expression of terpene biosynthetic genes including CaTPS10-like, a limonene synthase, resulted in the greater abundance of limonene in green beans, roasted beans and a unique citrus note of the coffee drink.

Non-toxic orange carbon dots stimulate photosynthesis and CO2 assimilation in hydroponically cultivated green beans (Phaseolus vulgaris).

Continuous increasing leaf photosynthesis may enhance plant yield. As an evolutionary property, plants use less photosynthetic capacity than is theoretically possible. Plant nanobionics is a bioengineering field that improves plant functions using nanoparticles. We applied orange carbon dots (o-CDs) onto the foliage of green beans (Phaseolus vulgaris ) grown in hydroponics to improve their photosynthetic performance and CO2 assimilation. Photosynthesis parameters, photosynthetic pigments content, total phenolic content (TPC) and antioxidative activity (TAA) were measured. Results show that photosynthetic pigments remained unchanged, while photosynthesis was improved. Both o-CDs concentrations decreased TPC and TAA. The light response curve showed higher CO2 assimilation at both o-CDs concentrations, particularly at lower light intensity. Correlation analysis confirmed increased CO2 binding and assimilation at 1mg L-1 . This study demonstrated the potential of using o-CDs as a safe biostimulator through photosynthesis increase and CO2 assimilation without toxic effects on plants. This may stimulate yield increase that paves the way for their agricultural application.

Effect of biostimulants on growth and production parameters of green beans (Phaseolus vulgaris L.) cultivated under North African climate

In crop growing, biostimulants are currently used to improve the advance and yield of crops. These biostimulants showed successful effects in various crops against severe stress factors. Therefore, integrative studies are required to define the usage of such products in agriculture. This study investigated the valuable impacts of biostimulants on the growth parameters of green beans (Phaseolus vulgaris) in Morocco known for its harsh climatic conditions. Equally, we studied the effect on the soil characteristics. The growth parameters were the number, length, and weight of leaves and pods, and were measured after, 21, 29, 35, 42, and 49 days of cultivation. The soil parameters were organic matter, macro-elements, and microelements. The obtained results exposed that the number of leaves was enhanced by 52.95 % in plants treated with biostimulants, while the improvement of foliar weight was estimated at 83.45 %. The treatment improved the weight of pods by 50 % after 75 days and by 63.65 % after 90 days. Similarly, the treatment improved the length of pods by 48 % after 75 days and by 78 % after 90 days. The improvement of growth parameters was in a time-dependent manner. These results are the first to clarify the faculty of biostimulants to enhance the growth performance of green beans under North African climatic and soil circumstances. However, more experiments are desired to evaluate the optimum quantities of biostimulants needed to get the maximum yield. Equally, future research is required to assess the influence of biostimulants on the nutritive quality of fruits.

Simultaneous Extraction and Decaffeination Process Optimization of Green Coffee Bean-Based Beverages Using Response Surface Methodology

Simultaneous Extraction and Decaffeination Process Optimization of Green Coffee Bean-Based Beverages Using Response Surface Methodology

Сортоизучение пиона древовидного Paeonia x suffruticosa Andr. при выращивании в условиях Московской области

The work is devoted to the variety study of tree peonies when grown in open ground conditions in the Moscow region. During the research, varieties with different growing seasons were identified, the duration of the flowering period was established, varieties with maximum values in height, plant diameter, number of flowers and flower size were identified, the most decorative varieties of tree peony were identified. The varieties Green Beans, Snow Fortress and Shima Nishiki bloomed first – 08.06, the varieties Midday Heat, Anastasia Sosnovets and Helen Martin – 09.06, 10.06 and 11.06, respectively. The longest flowering time for the varieties Midday Heat, Snow Fortress and Shima Nishiki is 9 days. The long duration of the growing season was noted in the varieties Snow Fortress, Green Beans, Midday Heat and Shima Nishiki, 118 days. The decorative variety is Shima Nishiki, which scored 64 points due to its originality. Anastasia Sosnovets and Helen Martin varieties are recommended for use in single and group plantings. Early varieties of Snow Fortress and Green Beans can be planted in flower beds, mixborders and in single plantings. When creating rock gardens and English gardens, it is possible to use the Midday Heat. To create rocky gardens, stylized Chinese and Japanese gardens, it is recommended to plant the Shima Nishiki variety. Keywords: TREE PEONY, VARIETIES OF TREE PEONY, CULTIVATION OF TREE PEONY, DECORATIVE PEONY, ASSESSMENT OF DECORATIVE PEONY

Cost and Income Structure of Organic and Conventional French Bean (Phaseolus vulgaris) Cultivation: A Case Study of Himachal Pradesh, India

The objective of this study was to investigate the cost and income structure of organic and conventional French bean cultivation in Solan district, Himachal Pradesh, in the 2018/ 2019 crop year. The sample included 80 farmers selected using a purposive sampling method, consisting of 40 organic and 40 conventional French bean growers. Survey questionnaires were used as the main instrument for data collection. Descriptive statistics and cost and income analysis were used for data analysis. The results indicate that the cost of production was higher, and output was lower under organic bean cultivation. Despite this, organic bean cultivation was more profitable than conventional farming, which was attributed to the higher prevailing market price for organic beans. Organic growers encounter numerous challenges and issues when cultivating and marketing of vegetables. Farmers seek a variety of aid from the government, business sector, and co-operative organizations to solve all of these challenges.

The Sweet Revolution: Sustainable Development of Honey Sector in Rajasthan, India- a Value Chain Based Approach

The primary objective to undertake this current study is to examine the Honey sector, specifically in the Rajasthan region of India. In particular, a value chain based approach is utilized to understand the existing challenges and prospects of the sector. In addition, the study aims to provide recommendations to strengthen the production, distribution, and sustainable development of Honey sector. India, being an agro economy, agriculture and allied activities constitute a substantial chunk of India’s GDP. Rajasthan, the largest state in terms of area in India, is well-known because of its abundant biodiversity and is acknowledged as the top crop producer, accounting for an estimated 50% of the country's supply of seed spices. Drawing from the body of existing literature, this study demonstrates how the Indian honey industry has not been able to live up to its potential and instead has fallen short of expectations. These factors include a lack of technological adoption, access to markets, and value chain development. A lack of governmental support, a lack of systematic marketing and distribution strategies, a lack of knowledge about beekeeping, limited access to sophisticated tools and technology integration, and a lack of chemicals required for efficient beekeeping and honey processing operations are the main obstacles impeding the rapid development of the honey's consumption industry. The primary approaches envisioned for the long-term growth of the honey industry should include effective methods of delivering components, knowledge-based methods of processing honey from bees, integration of processes that add value, and creation of market connections for local farmers, beekeepers, and honey producers. As a result, honey producers make more money while supporting the industry's participants' performance, profitability, and sustainable way of life. The findings of this study provide policymakers and government agencies with ideas on how to create programs for the long-term growth of Rajasthan’s honey sector.

Performance of different Varieties of French Bean (Phaseolus Vulgaris L.) under Prayagraj Agro-Climatic Condition

The present investigation was carried out at the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj (UP) during the rabi season 2022-23 to check the performance of different varieties of French Bean under Paryagraj agro -climate condition and to estimate economics of French Bean using different varieties. The experiment comprised of total 7 varieties V1 (IET/2022/FBBVAR-1), V3 (IET/2022/FBBVAR-3), V4 (IET/2022/FBBVAR-4), V5 (IET/2022/FBBVAR-5), V6 (IET/2022/FBBVAR-6), V7 (Lakshmi), V8 (Ellora No.11) consisting 3 replication which was laid out in randomized block design. From the above experiment finding it is concluded that all the parameter varied significantly. Further, while studying the plant height, no. of leaves and pod length was found maximum in (Lakshmi). Day to germination days, first flowering, 50% flowering and first pod picking were found minimum in (IET/2022/FBBVAR-1). Number of branches, pod girth, pod per plant, average pod weight, ascorbic acid, yield per ha and benefit-cost (B: C) ratio was found maximum in (IET/2022/FBBVAR-3). The maximum TSS, fiber and protein was found in (IET/2022/FBBVAR-1).

Environmental, social and economic sustainability in Lao coffee

Laos has experienced an emergence of coffee cultivation as Loa farmers, many of them women, have been cultivating coffee in the understorey of natural forests. This form of agroforestry produces high-quality specialty coffee, earning farmers a significant income while preserving biodiversity. Farmers’ adoption of agroforestry, in place of traditional shifting agriculture has been supported by initiatives like the Swiss Agency for Development and Cooperation (SDC) project, which establishes agroforestry learning centres and mini-processing centres, in collaboration with companies such as the MX Coffee Company and the Comma Coffee Company. Developments in coffee cultivation have been on the rise in the last three decades in Keoset, a community in Khoun District, where failed development projects were later revived by SDC and improved upon with market-oriented initiatives. The project has assisted in establishing coffee nurseries, providing training, and establishing partnerships with private companies for processing and marketing and as a result, farmers have advanced from selling unprocessed cherries to selling parchment (semi-processed beans), earning them a higher income. Farmers selling green beans are also collecting a premium price from roasting companies and exporters. The ability to generate an income from coffee cultivation is significant, because 90% of the farmers engaged in coffee cultivation are women, who are now able to depend on an independently earned income, granting them a greater voice in household decision-making. Climate change poses potential risk to the future of coffee production, but the protection offered by forests largely shields coffee crops from storms and changes in temperature. The forests' maintenance of biodiversity also protects the predators of pests and disease, ensuring no chemical methods are needed for pest control, including against the coffee cherry borer (Hypothenemus hampei). A variety of challenges remain for which there are recommended responses. Challenges of institutional and legal context require capacity building within the government; world price fluctuations necessitate that farmers practice a mixed farming system to not rely solely on coffee; geographical limitations promote a focus on specialty-grade coffee; and competition from other cash crops requires awareness of the environmental risks associated with other crops and stricter zoning and regulation over different production systems. It is worth heeding these recommendations, as agroforestry coffee systems enhance livelihoods, empower women, promote environmental sustainability and demonstrate resilience to climate change, making it a potential model for countries facing similar challenges in similar agroecological zones.

Pharmacological Activities, Therapeutic Effects, and Mechanistic Actions of Trigonelline.

Trigonelline (TRG) is a natural polar hydrophilic alkaloid that is found in many plants such as green coffee beans and fenugreek seeds. TRG potentially acts on multiple molecular targets, including nuclear factor erythroid 2-related factor 2 (Nrf2), peroxisome proliferator-activated receptor γ, glycogen synthase kinase, tyrosinase, nerve growth factor, estrogen receptor, amyloid-β peptide, and several neurotransmitter receptors. In this review, we systematically summarize the pharmacological activities, medicinal properties, and mechanistic actions of TRG as a potential therapeutic agent. Mechanistically, TRG can facilitate the maintenance and restoration of the metabolic homeostasis of glucose and lipids. It can counteract inflammatory constituents at multiple levels by hampering pro-inflammatory factor release, alleviating inflammatory propagation, and attenuating tissue injury. It concurrently modulates oxidative stress by the blockage of the detrimental Nrf2 pathway when autophagy is impaired. Therefore, it exerts diverse therapeutic effects on a variety of pathological conditions associated with chronic metabolic diseases and age-related disorders. It shows multidimensional effects, including neuroprotection from neurodegenerative disorders and diabetic peripheral neuropathy, neuromodulation, mitigation of cardiovascular disorders, skin diseases, diabetic mellitus, liver and kidney injuries, and anti-pathogen and anti-tumor activities. Further validations are required to define its specific targeting molecules, dissect the underlying mechanistic networks, and corroborate its efficacy in clinical trials.

The Effect of Type and Duration of Digestive Enzyme Treatment on Coffee Bean Composition

During kopi luwak production, coffee fruit is subjected to enzymatic and microbial treatment. While microbial modification of coffee fruit or beans is often investigated, there is little information regarding the influence of the enzymatic part of the process. In this study, green Arabica and Robusta beans were modified using basic animal digestive enzymes (pepsin or trypsin with α-amylase) and various treatment times (3, 6 or 12 h) to determine their effect on bioactive and other quality-affecting compounds. Analyses of coffee composition were performed using spectrophotometric and HPLC methods. Modified and control samples were significantly affected by the treatment. Among enzymes used, only proteases exhibited noticeable impact on target compounds by increasing soluble protein content in green beans. The most advantageous modification time was 3 h. The composition of beans was altered by the roasting step, with the effect not quite corresponding to the previous stage. In conclusion, enzymatic treatment of green coffee beans provides a way to alter coffee composition, which can further influence its quality.

Biochar has positive but distinct impacts on root, shoot, and fruit production in beans, tomatoes, and willows

Positive relationships have been documented between the amount of biochar added to soils and various aspects of plant growth and fertility such as root, shoot, and fruit production. However, these effects depend on biochar source materials, soil characteristics and species of plant examined. This makes it impossible to systematically compare and generalize findings across previous studies that have used different soils and biochar. We conducted a novel investigation to assess the effects of a single source of biochar (hazelnut wood), in a constructed organic soil, on the different plant tissues in three functionally distinct species: tomatoes (Solanum lycopersicon), green beans (Phaseolus vulgaris), and willow (Salix sp.). Five levels of biochar soil amendment were assessed: 0% (control), 3, 9, and 26% by dry weight. We found a highly significant positive relationship between biochar concentration and total plant biomass (roots + shoots + fruits) in all species, with no significant difference in total biomass response among species. Fruit production increased with increased biochar in both beans and tomatoes. However, tomatoes exhibited significant differences in response among plant tissues; fruit production and shoot biomass increased significantly with biochar, but root tissue did not. Bean germination success increased significantly with biochar concentration. Date of first flowering was earlier with increasing soil biochar in beans but not in tomatoes. Control over both sources of biochar and soil composition in this experiment enables us to conclude that biochar addition can have different impacts on different plants and, in some cases, species-specific impacts on different plant tissues and other measures of fertility. Our results are contrary to prior research that found inhibiting effects of biochar at levels comparable to our 26% treatment. Biochar impacts on soil properties such as CEC and percent base cation saturation do not explain our findings, leading us to conclude that microbial interaction with biochar is an important factor that may explain the positive impacts of soil biochar on plant fertility observed. Further research that repeats this experiment in other soil types, with other biochar sources, and with other plant species is necessary to determine the generalizability of these important findings.

Metabolite Profiles of Sukabumi Arabica Green Coffee Beans Evaluated by 1H NMR-Based Metabolomics

Although Sukabumi arabica coffee is one of the famous coffees in Indonesia, however its chemical information is still limited in the literature. In this work, the metabolite profiles of arabica green coffee beans obtained from various plantations in Sukabumi, including Ciayunan, Pondok Halimun, and Selabintana were evaluated by 1H NMR-based metabolomics. In total, 19 metabolites were successfully identified, including the major and minor metabolites of the coffee. The score plot of the OPLS-DA (Orthogonal Partial Least Square Discriminant Analysis) model was successful in classifying the metabolite profiles of the coffees based on their origins. The loading plot analysis showed that the signals belonging to fatty acids, sucrose, trigonelline, chlorogenic acid, lactic acid, and quinic acid, contributed to the classification. S-plot analysis revealed that Selabintana coffee was characterized with higher concentrations of trigonelline and sucrose, whereas Ciayunan sample had higher levels of fatty acids. Meanwhile, the metabolite profile of Pondok Halimun coffee demonstrated an intermediate characteristic between the Ciayunan and Selabintana samples. This work provided valuable scientific information for coffee development especially in West Java and generally in Indonesia.
 
 Keywords: metabolomics, multivariate data analysis, NMR, Sukabumi arabica green coffee beans.