Many nurses today are religious and sometimes there exist some religious motivations on becoming a nurse. They are often responsible through their care to assess and facilitate spiritual well-being, identify spiritual distress and to provide religious and spiritual care. Nurses need to understand their own spirituality and religiosity before they can successfully integrate them into their care. Aim: The present study aims to assess Greek nurses’ religiosity and to validate the Centrality of Religiosity Scale (CRS) in the Greek language. Material and Methods: The CRS-15 questionnaire is an anonymous self-administered questionnaire that contains fifteen, five point Likert scale, closed questions (ranging 1-5). The sample of the study was 344 nurses and nurse assistants. Exploratory factor analysis, with principal components analysis, was performed for checking the construct validity of the questionnaire. The test–retest reliability and the internal consistency were also examined. Statistical analysis performed using SPSS 21.0. Statistical significance level was set at p=0.05. Results: The final Greek version of the questionnaire includes all the fifteen questions. The mean age of the participants was 42.9±7.5. Two factors exported from the statistical analysis: the first one corresponded to religious practices and the second one to religious beliefs and experiences. The Cronbach-a coefficient was 0.952 for the total questionnaire and for Religious beliefs and experiences is a=0.923 while for the religious practices is a=0.926. Conclusions: The CRS-15, is a valuable and reliable questionnaire that can be used for assessing religiosity in Greek population.