Greater Shortening Research Articles

Optimum distribution of additional reinforcement to reduce differential column shortening

SummaryDifferential column shortening should be reduced to mitigate the adverse effects caused by such shortening in a tall building. The axial stiffness of columns with greater shortening than adjacent columns should be increased to reduce the differential column shortening. The axial stiffness of the columns can be effectively increased by utilizing additional reinforcement for them. The optimum distributions of this additional reinforcement to reduce the differential column shortening in a tall building are determined. An optimization problem is formulated to minimize the total amount of reinforcement. Three analysis models, namely a fixed‐section, fixed‐stress and general model, are presented as numerical examples. The contour plots for a two‐variable problem of the three analysis models are presented. The generalized reduced gradient method is applied to obtain the optimum solutions of multi‐variable problems. The differential column shortenings after optimization confirm that the optimization program developed in this study can yield the optimum distributions of additional reinforcement. Copyright © 2015 John Wiley & Sons, Ltd.

Along-strike variability of thrust fault vergence

The 3D kinematic evolution of thrust systems, in which vergence changes along strike, is poorly understood. This study uses 3D seismic data from Big Piney-LaBarge field, Wyoming, to examine the geometry and kinematics of two faults at the leading edge of the Hogsback thrust sheet, the frontal thrust of the Late Cretaceous Sevier fold-thrust belt. These thrusts lie along strike of each another and share an east-vergent detachment within the Cretaceous Baxter Shale. The two thrusts verge in opposite directions: The southern thrust verges eastward forming a frontal ramp consistent with major thrusts of the Sevier belt, whereas the northern thrust verges westward to form a type 1 triangle zone with the Hogsback thrust. The thrusts have strike lengths of 5 km (3.1 mi) and 8 km (5.0 mi), respectively, and they are separated by a transfer zone of less than 0.5 km (0.3 mi) wide. Strata in the transfer zone appear to be relatively undeformed, but reflections are less coherent here, which suggests small offsets unresolved by the seismic survey. Retrodeformable cross sections and a structure contour map on the Cretaceous Mesaverde Group indicate that shortening varies along strike, with a pronounced minimum at the transfer zone and greater shortening across the northern, west-vergent thrust (610 m [2000 ft]) than across the southern, east-vergent thrust (230 m [755 ft]). Mapping of these thrusts suggests that they propagated laterally toward each other to form a type 1 antithetic fault linkage in the transfer zone. Spatial patterns expressed in seismic attributes in and near the detachment horizon, which include waveform classification and spectral decomposition, suggest that stratigraphic variations may have pinned the detachment, thus localizing the transfer zone. Thickness variations in the thrust sheet also may have influenced the thrust geometry. Our study provides an analog for analysis of similar complex contractional belts around the world.

Reduction of differential column shortening by placing additional reinforcement

A method to reduce differential column shortening in reinforced concrete (RC) tall buildings by placing additional reinforcement at the columns is proposed. The axial stiffness of columns with greater shortening than adjacent columns connected through horizontal members should be increased to reduce differential column shortening. The axial stiffness of the columns can be effectively increased by placing additional reinforcement at the columns. Factors that affect the efficiency of reinforcement at reducing column shortening were investigated through single-column analysis, sensitivity analysis of a multi-storey column line and a comparison of three distribution schemes. A step-by-step method for the long-term analysis of RC structures is applied for the exact column shortening for any elapsed time. Based on the results, differential column shortening can be reduced in the design stage by placing additional reinforcement at the columns.

Development of antheridial filaments of Chara vulgaris L. in isolated antheridia in vitro

The study was undertaken to establish the conditions of culture <em>in vitro</em> of <em>Chara vulgaris</em> L. antheridium isolated from the mother plant and to ascertain the regularities in the development of the antheridial filaments under these conditions. The culture of isolated antheridia was run for 5 days on Forsberg medium. The antheridial filaments were found to preserve their full viability evaluated in terms of <sup>3</sup>H-phenylalanine incorporation With simultaneous depression of mitotic activity. Under these conditions a considerable part of the filaments pass through at least three division cycles. <em>In vitro</em> culture of isolated antheridia causes the greater shortening of the cell length the longer the antheridia are kept Isolated from the thallus.

Microsatellite Instability Affecting the T17 Repeats in Intron 8 of HSP110 , as Well as Five Mononucleotide Repeats in Patients with Colorectal Carcinoma

To investigate mononucleotide markers: BAT-25, BAT-26, NR-21, NR-22 and NR-24 in patients with colorectal cancer (CRC), and the status of HSP110T17, KRAS, BRAF and the MLH1 promoter mutations in microsatellite unstable CRC. Genetic assessments were performed on samples obtained following resection of CRC in 200 patients. Allelic variations of HSP110T17 were found in all 18 patients with microsatellite instabilities (MSIs) in at least three markers (high-frequency MSI). By contrast, mutations of HSP110T17 were absent in all 20 patients with no MSI frequency. Eight out of 182 patients with low (instability in one marker) or no frequency MSI had allelic shifts due to polymorphisms of BAT-25 (1.5%), NR-21 (1.75%) and NR-24 (1.5%). BRAF mutations were associated with >5 bp shortening of HSP110T17. Patients with high-frequency MSI CRC had allelic variations of HSP110T17. BRAF mutations occur along with greater shortening in HSP110T17 during oncogenesis via the MSI pathway.

Effect of arm swing on effective energy during vertical jumping: Experimental and simulation study

Arm swing helps to increase vertical jump height (VJH), in part by a greater hip joint muscle work. The force-velocity relationship has been put forward to explain the increase in hip joint work. Nevertheless, the efficacy ratio, muscle shortening length, and active state might be parameters that affect the effective energy and then VJH. The purpose of this study was to evaluate the influence of arm swing on effective energy in vertical jumping. Eight subjects performed maximal squat jumps with (SJ arm ) and without arm swing (SJ). A simulation model of the musculo-skeletal system was applied. For subjects and simulation, VJH was about 20% higher during SJ arm. In subjects, this was explained by the shoulder joint work (34%) and an increase of L5-S1 joint work (66%). In simulated jump, effective energy increase during SJ arm (+80.74 J) was related to an improvement of the total muscle work and not to the efficacy ratio. The increase in total muscle work was due to anterior deltoid work and to greater erector spinae, biceps femoris, and gluteus work. The greater muscle works were explained by a slower shortening velocity for all the muscles and by a greater shortening length and active state for the biceps femoris.

Multivariate statistical analysis of deformation tensors: independent vs. correlated tensor observations

In the presence of errors in measuring a random displacement field (under the normal distribution assumption of displacement field), stochastic behaviors of principal components of deformation tensors (strain tensor and tensor of change of curvature (TCC)), based on the intrinsic assumption of geometrical modeling of surface deformation analysis, are discussed. We divided the contents into two parts: In the first, we considered independent random vectors of repeated tensor measurements. In the second step, we considered correlations among repeated measurements. Then, covariance components between tensor elements by Helmert estimator, based on prior information of variance components, are estimated. As a case study, both assumptions are applied to the estimation of principal components of deformation rate tensor observations in Zagros region (Western Iran). The results of numerical analysis showed that greatest shortening is accommodated in oblique orientation (NS) with respect to the Main Recent Fault (MRF), northwest part of North Zagros, Central Iran block and MRF, respectively. Most of the extensions occurred in the east part of the belt. The pattern of eigenspace components of TCC shows highest positive values across the NW region, nearly in orthogonal direction to the MRF and Main Zagros Fault (MZF). The pattern has insignificant values in the Central Zagros. It takes the significant negative values across the SW part, especially along the SPF and Persian Gulf shore. The effect of non-independent observations on the estimation of eigenspace components of deformation tensors (strain tensor and TCC) shows that the estimation of covariance components has influence on the confidence intervals of eigenspace components, especially in seismically active regions of the belt (along the Persian Gulf shore, NW of the belt and region between the Central Iran block and MRF). The results demonstrate the importance of considering the correlation structure among the observations on statistical behavior of principal components of deformation tensors in seismically active regions.

Maize leaf development under climate change scenarios

The objective of this work was to simulate maize leaf development in climate change scenarios at Santa Maria, RS, Brazil, considering symmetric and asymmetric increases in air temperature. The model of Wang & Engel for leaf appearance rate (LAR), with genotype-specific coefficients for the maize variety BRS Missões, was used to simulate tip and expanded leaf accumulated number from emergence to flag leaf appearance and expansion, for nine emergence dates from August 15 to April 15. LAR model was run for each emergence date in 100-year climate scenarios: current climate, and +1, +2, +3, +4 and +5°C increase in mean air temperature, with symmetric and asymmetric increase in daily minimum and maximum air temperature. Maize crop failure due to frost decreased in elevated temperature scenarios, in the very early and very late emergence dates, indicating a lengthening in the maize growing season in warmer climates. The leaf development period in maize was shorter in elevated temperature scenarios, with greater shortening in asymmetric temperature increases, indicating that warmer nights accelerate vegetative development in maize.

Cardiomyocyte Overexpression of Neuronal Nitric Oxide Synthase Delays Transition Toward Heart Failure in Response to Pressure Overload by Preserving Calcium Cycling

Defects in cardiomyocyte Ca(2+) cycling are a signature feature of heart failure (HF) that occurs in response to sustained hemodynamic overload, and they largely account for contractile dysfunction. Neuronal nitric oxide synthase (NOS1) influences myocyte excitation-contraction coupling through modulation of Ca(2+) cycling, but the potential relevance of this in HF is unknown. We generated a transgenic mouse with conditional, cardiomyocyte-specific NOS1 overexpression (double-transgenic [DT]) and studied cardiac remodeling, myocardial Ca(2+) handling, and contractility in DT and control mice subjected to transverse aortic constriction (TAC). After TAC, control mice developed eccentric hypertrophy with evolution toward HF as revealed by a significantly reduced fractional shortening. In contrast, DT mice developed a greater increase in wall thickness (P<0.0001 versus control+TAC) and less left ventricular dilatation than control+TAC mice (P<0.0001 for both end-systolic and end-diastolic dimensions). Thus, DT mice displayed concentric hypertrophy with fully preserved fractional shortening (43.7+/-0.6% versus 30.3+/-2.6% in control+TAC mice, P<0.05). Isolated cardiomyocytes from DT+TAC mice had greater shortening, intracellular Ca(2+) transients, and sarcoplasmic reticulum Ca(2+) load (P<0.05 versus control+TAC for all parameters). These effects could be explained, at least in part, through modulation of phospholamban phosphorylation status. Cardiomyocyte NOS1 may be a useful target against cardiac deterioration during chronic pressure-overload-induced HF through modulation of calcium cycling.

Effects of concurrent cognitive task on pre-landing muscle response latency during stepping down activity in older adults with and without a history of falls

Purpose. To investigate the co-contraction of ankle muscles in older subjects with and without a history of falls during a stepping down activity, and whether the co-contraction was disrupted by a concurrent cognitive task.Method. Ten fallers and 9 non-fallers (mean age = 72.0 ± 5.0 and 72.1 ± 7.3 years, respectively) were recruited. Electromyography (EMG) of the tibialis anterior (TA) and medial gastrocnemius (MG) was recorded during stepping down with and without a concurrent cognitive task. Co-contraction was investigated using the time between the EMG onset and the foot touching a force-platform, termed the pre-landing muscle response latency.Results. The fallers showed longer pre-landing muscle response latencies in the TA compared with non-fallers (141.1 ± 58.1 ms and 110.9 ± 68.2 ms, respectively). With a concurrent cognitive task, the pre-landing muscle response latencies in the TA were shortened in fallers significantly more than in non-fallers (44.4% and 15.5%, respectively). No significant difference in MG activation was found between 2 groups in the stepping down activity with and without cognitive task.Conclusions. Subjects with history of falls exhibit a greater shortening in the pre-landing muscle response latency than non-fallers when distracted during stepping down. Disruption of their co-contraction in ankle joint might precipitate such older adults to fall.

Comparison of CDR3 length among thymocyte subpopulations: Impacts of MHC and BV segment on the CDR3 shortening

Thymocytes are thought to be selected on the basis of antigen specificity between TCR and peptide-MHC (pMHC) ligands. The specificity depends primarily on extensive diversities of complementarity determining region 3 (CDR3), whose specificity is considered to be determined through thymocyte selection. We examined the CDR3 length profiles with 20 BV segments in thymocyte subpopulations from C57BL/6 (H-2(b)), C.B10 (Balb/c congenic, H-2(b)) and Balb/c (H-2(d)) mice. The CDR3 length was shorter in both CD4 single positive (SP) and CD8SP than in double positive (DP), but not altered among DP, double negative (DN) 4 and DN3 subpopulations. The CDR3 shortened more prominently in CD4SP than in CD8SP for C57BL/6 and C.B10, but the shortening was only slight for Balb/c. Although the shortening varied considerably among different BV segments, the greater shortening was observed in most BV segments for CD4SP and in several for CD8SP, in particular, the extent was the greatest in BV1, BV2, BV15, BV16, BV23 and BV26 for CD4SP, and in BV13-1 and BV29 for CD8SP. Moreover, the extent and the pattern of CDR3 shortening were basically the same among highly homologous BV segments (e.g. BV12-1 and 12-2; BV13-1, 13-2 and 13-3). These results taken together indicate that (1) the CDR3 shortening occurred between the DP to the SP stages but never earlier, that (2) there would be the MHC class preference for the CDR3 shortening, that (3) it was in part influenced by MHC haplotype, and finally that (4) the primary structure of particular BV segments would possibly affect the CDR3 length in selected thymocytes. It could be deduced from these results that the CDR3 shortening might play roles in ensuring geometrical disposition of CDRs unique to each BV segment and consequently allow CDRs to intimately interact with pMHC ligands.

Electrical and hemodynamic function produced by stimulation of atropine sensitive right ventricular nerves in humans

In mammalian ventricles including humans, it is recognized that parasympathetic ganglia innervate the heart. Little is known about the location and function of right ventricular parasympathetic nerves in humans. We hypothesized that in humans: (1) there are parasympathetic ganglia that supply the right ventricle that can be stimulated via an endocardial catheter and (2) stimulation of these fibers will alter the electrical and hemodynamic function of the right ventricle. Parasympathetic nerve stimulation was performed via an endocardial catheter placed along several sites of the right ventricle, superior vena cava, and right internal jugular area in humans. The spatial extent of parasympathetic innervation was mapped in 1-cm zones across the right ventricle. Cardiac output, heart rate, and atrioventricular conduction were monitored to provide independent assessment of parasympathetic innervation. In all 22 patients, ventricular refractoriness shortened from 12 +/- 3 to 3 +/- 1 ms during parasympathetic nerve stimulation, and the greatest shortening of refractoriness was observed at the base of the right ventricle (p = 0.01). No significant shortening in ventricular refractoriness occurred in areas beyond 2 cm from the right ventricular base. These results were compared by using T table test. The parasympathetic nerve stimulation protocol decreased cardiac output, reaffirming the principle effect of parasympathetic ganglia. Atropine was administered in seven patients. All effects from nerve stimulation were abolished after atropine administration. These results were also compared by using T table test. These data provide the first demonstration of the electrical and hemodynamic function by stimulation of atropine sensitive nerves of the human right ventricle. Greater understanding of parasympathetic innervation may lead to novel therapies for arrhythmias.

Muscle Shortening Along the Normal Esophagus During Swallowing

Longitudinal shortening of the esophagus during peristaltic contraction has been previously analyzed globally using spaced mucosal clips. This method gives a relatively crude measurement. In this study, local longitudinal shortening (LLS) was evaluated using simultaneous high-resolution endoluminal ultrasound (HREUS) and manometry based on basic principles of muscle mechanics. We sought to determine if there are regional differences in LLS of the esophageal muscle during swallow-induced peristaltic contraction and evaluate shortening of the circular smooth muscle (CSM) and longitudinal smooth muscle (LSM) of the esophagus. Twenty normal subjects underwent simultaneous HREUS/manometry at 4 levels (5, 10, 15, and 20 cm above the upper border of the lower esophageal sphincter [LES] high-pressure zone) in the esophagus with 5-mL swallows of water. Ultrasound images were recorded with synchronized manometric pressure data. The images were digitized and the cross-sectional surface area (CSA) of the LSM, CSM, and total muscle (TM) were measured at baseline (at rest) and at peak intraluminal pressure (implying peak CSM contraction) during swallowing. LLS was calculated for the CSM and LSM using the principle of mass conservation, whereby the change in CSA relative to the resting CSA is quantitatively equal to the relative change in length of a local longitudinal muscle segment.CSM, LSM, and TM all shortened longitudinally, with the circular muscle shortening more than the longitudinal muscle, LLS of the CSM and TM layers at 5 cm above the LES was significantly greater than at 20 cm (CSM: 30% difference, P < .001; TM: 18% difference, P < .05). The greater shortening of LSM at 5 versus 20 cm was found not to be statistically significant (11% difference, P > .05). Peak intraluminal pressure strongly correlated with peak muscle thickness of all layers at all levels (r = 0.96-0.98).LLS increases from the proximal to the distal esophagus during bolus transport. CSM and LSM both shorten longitudinally, with CSM shortening more than LSM. The increase in LLS increases the efficiency of peristaltic contraction and likely contributes to the axial displacement of the LES preceding hiatal opening and esophageal emptying.

γ-Sarcoglycan deficiency increases cell contractility, apoptosis and MAPK pathway activation but does not affect adhesion

The functions of gamma-sarcoglycan (gammaSG) in normal myotubes are largely unknown, however gammaSG is known to assemble into a key membrane complex with dystroglycan and its deficiency is one known cause of limb-girdle muscular dystrophy. Previous findings of apoptosis from gammaSG-deficient mice are extended here to cell culture where apoptosis is seen to increase more than tenfold in gammaSG-deficient myotubes compared with normal cells. The deficient myotubes also exhibit an increased contractile prestress that results in greater shortening and widening when the cells are either lightly detached or self-detached. However, micropipette-forced peeling of single myotubes revealed no significant difference in cell adhesion. Consistent with a more contractile phenotype, acto-myosin striations were more prominent in gammaSG-deficient myotubes than in normal cells. An initial phosphoscreen of more than 12 signaling proteins revealed a number of differences between normal and gammaSG(-/-) muscle, both before and after stretching. MAPK-pathway proteins displayed the largest changes in activation, although significant phosphorylation also appeared for other proteins linked to hypertension. We conclude that gammaSG normally moderates contractile prestress in skeletal muscle, and we propose a role for gammaSG in membrane-based signaling of the effects of prestress and sarcomerogenesis.

Cervical length at 11–14 weeks' and 22–24 weeks' gestation evaluated by transvaginal sonography, and gestational age at delivery

To compare cervical length measurements obtained at 11 to 14 weeks and 22 to 24 weeks of gestation in an unselected group of pregnant women and to correlate the measurements with time of delivery. This was a prospective study involving 529 pregnant women attending for routine antenatal care who underwent transvaginal scans at 11-14 weeks and 22-24 weeks for evaluation of cervical length. The mean cervical length was calculated at both stages of gestation and lengths were compared between groups which delivered at term or prematurely, this being defined as delivery before 37 completed weeks of gestation. The mean cervical lengths at 11-14 and 22-24 weeks were, respectively, 42.4 mm and 38.6 mm. Cervical length at 11-14 weeks was not significantly different between the groups which delivered at term (42.7 mm) and preterm (40.6 mm). However, at the 22-24-week evaluation, cervical length was significantly shorter in the group which had a preterm delivery than in that which had a term delivery (26.7 mm and 39.3 mm, respectively; P = 0.0001). In the group of women with a previous history of one or more preterm deliveries, there was a greater shortening in cervical length from the first to the second evaluation than there was in the group of women with no previous history of preterm delivery. This shortening was also more pronounced in the group which delivered prematurely (from 40.6 mm to 26.7 mm) than in that which delivered at term (from 42.7 mm to 39.3 mm). There is a spontaneous shortening in the pregnant cervix from the first to the second trimester of pregnancy. The shortening is more rapid in pregnant women who deliver prematurely and who have a history of previous preterm delivery.

Arthroscopic diagnosis of intra-articular soft tissue injuries associated with distal radial fractures

Arthroscopy was used to assess the soft tissue injuries associated with distal radial fractures in 118 acute intra- and extra-articular fractures. The triangular fibrocartilage complex (TFCC) was torn in 46 of 118 patients--in 35% of intra-articular fractures and in 53% of extra-articular fractures. No correlation between ulnar styloid fractures and TFCC injuries could be found. Scapholunate (SL) ligament injuries with instability were present in 21.5% of intra-articular fractures and in 6.7% of extra-articular fractures. Lunotriquetral (LT) ligament injuries with instability were present in 6.7% of intra-articular fractures. and in 13.3% of extra-articular fractures. Combined SL and LT injuries were present in 5.6% of intra-articular fracture. Preoperative radiographs correlated with TFCC injury. Patients with TFCC tears had greater shortening and dorsal angulation on the preoperative radiographs. Preoperative radiographs had no predictive value for interosseous ligament injury. Ligamentous injuries are commonly associated with both intra-articular and extra-articular distal radial fractures.

The role of Na(+)-Ca2+ exchange in activation of excitation-contraction coupling in rat ventricular myocytes.

1. The purpose of this study was to determine whether mechanisms other than Ca2+ influx via L-type Ca2+ current (ICa) might contribute to activation of contraction in rat ventricular myocytes. The whole-cell voltage-clamp technique was used with normal transmembrane K+ and Na+ gradients at 34 degrees C. The sarcoplasmic reticulum (SR) was conditioned with one to three prepulses to +100 mV for 100 ms. 2. Cell shortening (delta L) increased with test voltage up to a plateau level at about +20 mV, beyond which cell shortening remained fairly constant, thus describing a sigmoidal voltage dependence. This relationship was obtained when holding potential (Vh) was either -40 or -70 mV; however, greater shortening was obtained at the more negative Vh. 3. The sigmoidal V-delta L relationship was converted to a bell shape following the magnitude of ICa when internal Cs+ was substituted for K+ and when the temperature was reduced to 22 degrees C. 4. At 34 degrees C, block of ICa with nifedipine (10 microM) decreased shortening by about 50% but did not alter the voltage dependence of delta L when Vh was either -40 or -70 mV. Addition of Ni2+ (4-5 mM) blocked all remaining contractions. 5. When cell shortening was triggered by an action potential voltage clamp, there was again about 50% of the contraction that was insensitive to nifedipine but was blocked by Ni2+. 6. Our results demonstrate that there is a significant contribution of a nifedipine-insensitive mechanism to the activation of contraction. This mechanism is likely to be reverse mode Na(+)-Ca2+ exchange since it appears to be sensitive to both voltage and Ni2+. We conclude that a contribution of reverse Na(+)-Ca2+ exchange to activation of excitation-contraction coupling occurs in rat heart at near-physiological conditions which include warm temperatures, normal transmembrane Na+ and K+ gradients and activation in response to an action potential.

Age-related changes in electrophysiological responses to muscarinic receptor stimulation in rat myocardium.

Recent studies have demonstrated that the negative chronotropic and inotropic responses of the heart to cholinergic muscarinic receptor stimulation are strikingly enhanced with aging in the rat model. The present study investigated the electrophysiological basis of this phenomenon by determining the effects of a muscarinic receptor agonist, carbachol, on transmembrane action potential parameters in right atrial tissue and right ventricular free wall preparations from adult (6-8 months old) and aged (26-28 months old) Fischer 344 rats. In addition, the effect of carbachol on atrioventricular conduction time (AVT) was determined in isolated perfused beating hearts. The results showed the following. The baseline maximum diastolic potential (MDP: adult, -76.4 +/- 1.8 mV; aged, -66.8 +/- 1.5 mV; p < 0.05; n = 5) but not the action potential duration measured at 95% repolarization (APD95: adult, 40.0 +/- 5.0 ms; aged, 47.4 +/- 6.7 ms; n = 5) differed significantly in aged compared with adult atrium. No significant age-related difference was evident in baseline MDP measured in ventricular epicardium (adult, -69.8 +/- 0.5 mV; aged, -69.0 +/- 1.1 mV; n = 6) or endocardium (adult, -72.5 +/- 1.4 mV; aged, -73.0 +/- 1.2 mV; n =6). The baseline action potential duration measured at 50% repolarization (APD50) differed significantly with age in ventricular endocardium (adult, 11.6 +/- 2.2 ms; aged, 23.0 +/- 4.6 ms; p < 0.05; n =6) but not in epicardium (APD50: adult, 8.1 +/- 0.4 ms; aged, 13.0 +/- 2.3 ms; n = 6). Superfusion with carbachol (0.1 nM - 10 mu M) resulted in concentration-dependent hyperpolarization of MDP in atrium; the magnitude of hyperpolarization differed significantly with age (2.5-fold higher in the aged; p < 0.05; n = 5). Carbachol caused concentration-dependent shortening of APD50; this effect differed significantly with age in the ventricle (2-fold greater in the aged; p < 0.05; n = 6) but not in the atrium. Carbachol prolonged the AVT in atrial-paced (240 beats/min) hearts; the magnitude of carbachol-induced increase in AVT did not differ significantly with age. These results are consistent with the possibility that in the aging heart, greater hyperpolarization at the level of the right atrium (likely involving pacemaker cells) and greater shortening of APD50 at the level of ventricular myocytes may contribute to the enhanced cholinergic-triggered bradycardia and negative inotropic response, respectively.

Effects of ACC (1-aminocyclopropane-1-carboxylic acid) applied through the roots of maize seedlings on vegetative and early reproductive development of the shoots

Maize plants, grown in aerated solution cultures, were exposed, at different growth stages, to ACC (1-aminocyclopropane-1-carboxylic acid) applied through the roots for up to 9 d. Total uptake of ACC increased with seedling size. During ACC treatment, ethylene evolution, by the shoots, proceeded at an almost constant rate per unit fresh weight that was up to 40-fold faster than that of untreated plants. This stimulation extended several days beyond the period of ACC uptake. The effects on growth and development were assessed when plants were 50–52-d old. ACC application shortened certain stem internodes, leaf-sheaths and laminae. The location of these effects depended on the time of application. The greatest shortening was induced by application, at the 4-leaf stage (10 d-old), prior to elongation of the cone of the shoot apex. This is ascribed to effects on meristematic tissue, in addition to those on elongating cells. An unexpected response to ACC treatment, at the 4-leaf stage, was an increase of up to four leaf-bearing stem nodes compared to untreated plants. This resulted in a parallel elevation of the uppermost ear-bearing axillary shoot to higher nodal positions. The length of leaves high in the canopy (nodes 11–16) was promoted by treating seedlings with ACC. The only clear effect of the ACC treatments on emergent axillary shoots per se was a retardation of silk elongation.

Mechanically induced changes in action potential duration and left ventricular segment length in acute regional ischaemia in the in situ porcine heart.

The electrophysiological events accompanying early ischaemia are important. The aim of this study was to investigate mechano-electric feedback in acute regional myocardial ischaemia in the intact heart in situ by measuring the change in action potential duration in response to increased ventricular loading imposed by transient aortic occlusion. 11 landrace pigs were anaesthetised and their hearts exposed. A pneumatically operated blood pressure clamp was placed around the aorta. Monophasic action potentials and an index of segment motion were recorded from the epicardium in and around the ischaemic area produced by a snare placed around a coronary artery. Ventricular and systemic arterial pressures were measured. An initial aortic clamp was performed during which control recordings were taken. The coronary artery was then tied and the aorta clamped for 5-10 s every 5 min for the duration of the 30 min tie. Recordings were taken from the ischaemic area and non-ischaemic areas. Aortic clamp before ischaemia increased intraventricular diastolic and systolic pressure and reduced action potential duration in all the areas studied (p < 0.001). During acute regional myocardial ischaemia aortic clamping resulted in significantly more shortening of the action potential in the ischaemic area after 10 min of ischaemia than in the control area (5 ms v 10 ms, p = 0.008). Over the following 20 min the degree of shortening decreased. The greater shortening at 10 min could not be attributed to changes in the end diastolic segment length or peak ventricular pressure and could thus represent a change in the expression of mechano-electric feedback by ischaemic myocardium rather than a change in loading conditions. During the first 30 min following a coronary artery occlusion mechano-electric feedback in the ischaemic myocardium varies with time.