Greater Injury Research Articles

Electrographic seizures are associated with brain injury in newborns undergoing therapeutic hypothermia

ObjectiveSeizures are common among newborns with hypoxic-ischaemic encephalopathy (HIE) but the relationship between seizure burden and severity of brain injury among neonates receiving therapeutic hypothermia (TH) for HIE is unclear....

Response of Dry Bean to Sulfentrazone Plus Imazethapyr

Field studies were conducted in 2010 and 2011 at the Huron Research Station, Exeter, Ontario and from 2009 to 2011 at the University of Guelph Ridgetown Campus, Ridgetown, Ontario to evaluate the sensitivity of four market classes of dry bean to sulfentrazone applied preemergence at 105, 140, and 280 g ai/ha alone and in combination with imazethapyr at 37.5 g ai/ha. At 1 week after emergence (WAE), sulfentrazone alone or in combination with imazethapyr at all doses evaluated caused no significant visible injury in dry bean. At 2 WAE, sulfentrazone alone caused 1–11, 1–11, 1–5, and 3–19% visible injury, and sulfentrazone + imazethapyr caused 3–11, 2–10, 2–5, and 4–20% visible injury in black, cranberry, kidney, and white bean, respectively. At 4 WAE, sulfentrazone alone caused 1–7, 1–7, 0–4, and 1–16% visible injury and sulfentrazone + imazethapyr caused 1–8, 1–5, 1–3, and 2–14% visible injury in black, cranberry, kidney, and white bean, respectively. Sulfentrazone PRE caused slightly greater injury in black and white bean compared to cranberry and kidney bean. Generally, crop injury with sulfentrazone at rates up to 140 g ai/ha alone and in combination with imazethapyr at 37.5 g ai/ha was minimal with no adverse effect on plant height, shoot dry weight, seed moisture content, and yield. Based on these results, there is potential for preemergence application of sulfentrazone at rates up to 140 g ai/ha alone or in combination with imazethapyr at 37.5 g ai/ha in black, cranberry, kidney and white bean.

Isoniazid-induced liver injury and immune response in mice

Isoniazid (INH) is associated with one of the highest incidences of idiosyncratic drug-induced liver failure of any commonly prescribed drug. The mechanism of this liver injury remains uncertain, and a valid animal model would greatly facilitate mechanistic studies. Most studies of INH-induced liver toxicity have been acute studies performed in rats with high doses of the drug, and this is very different from the idiosyncratic liver injury that occurs in humans. It has previously been demonstrated that covalent binding of INH in the liver of mice is greater than in rats and more like that in humans. Therefore, mice should be a better species in which to develop an animal model of INH-induced liver injury. Treatment of Cbl-b−/− and PD1−/− mice, which have impaired immune tolerance, resulted in greater injury than their C57BL/6 background, but not liver failure. This suggested that the injury was mediated by the adaptive immune system; however, Rag−/− mice, which do not have competent T- and B-cells, sustained more liver injury than C57BL/6 wild-type mice. This suggested that the adaptive immune system also played a protective role. INH treatment also led to a decrease in the inflammatory cytokines IL-1α and IL-12, which suggests that the drug may have immunosuppressive properties. In short, a mouse model was developed of INH-induced liver injury in which the immune system appears to play a both protective and pathogenic role, but this study was unable to develop a model of INH-induced liver failure.

Response of Processing Tomato to Simulated Bromoxynil Drift Followed by In-Crop Metribuzin Application

Simulated drift rates of bromoxynil followed by an in-crop application of metribuzin were applied to processing tomato in eight field studies conducted from 2008 to 2010 in Ridgetown, Ontario, Canada, to determine if a synergistic interaction occurred due to the cumulative herbicide application. A transient synergistic response was observed 7 d after treatment (DAT) when bromoxynil drift rates of 8.5, 17, and 34 g ai ha−1were followed 3 to 5 d later by metribuzin at 250 g ai ha−1. By 28 DAT, visible injury ratings were additive for 8.5, 17, and 34 g ha−1bromoxynil followed by metribuzin treatments. However, when bromoxynil at 68 g ha−1(20% of field rate) was followed by metribuzin, a synergistic interaction was evident and remained through harvest. Based on Colby's equation there was greater visible injury than expected at 7, 14, and 28 DAT when bromoxynil at 68 g ha−1was followed by metribuzin. A corresponding synergistic reduction of plant dry weight and marketable tomato yield, compared with the nontreated control, was identified. Marketable yields were expected to be 65% of the control according to Colby's equation, but observed yield reductions were 49% when bromoxynil at 68 g ha−1was followed by metribuzin. In general, tomato plants sprayed with metribuzin after bromoxynil drift had greater injury than treatments sprayed with bromoxynil alone.

Tolerance Evaluation of Vegetatively Established Miscanthus × giganteus to Herbicides

Giant miscanthus is under consideration as a biofuel crop in the United States; however, there is little information on weed management for the establishment and survival of this crop. Therefore, greenhouse and field studies using ornamental pots were conducted in summer 2011 at Tifton, GA, with the objective of screening potential PPI, PRE, and POST herbicides and herbicide combinations for giant miscanthus when establishing from vegetative rhizomes. For the POST treatments, giant miscanthus was established from rhizomes in 7.6-L containers in the field and treated with 27 POST herbicides to evaluate efficacy. Thifensulfuron, metsulfuron, tribenuron, chlorimuron, halosulfuron, rimsulfuron, cloransulam, pinoxaden, bentazon, and metribuzin did not significantly lower shoot height, reduce shoot dry weight, or increase injury compared with nontreated control (NTC) when evaluated at 4 wk after treatment. Nicosulfuron, trifloxysulfuron, sulfometuron, clodinafop, fluazifop, and pyrithiobac caused the greatest injury, reduced plant height, and reduced dry weights compared with the NTC. Sethoxydim, diclofop, flumioxazin, imazamox, imazapic, and imazethapyr decreased plant heights or resulted in increased injury. PPI and PRE treatments included 21 herbicides and herbicide combinations applied at two rates. Results indicated that most treatments containing atrazine, metribuzin, pendimethalin, acetochlor, metolachlor, and mesotrione did not injure or stunt growth; however, EPTC at 4.5 kg ai ha−1 significantly reduced height and dry weight and oxadiazon resulted in greater injury compared with NTC at both rates. These results indicate that PPI, PRE, and POST herbicides can be utilized for establishment of giant miscanthus from vegetative rhizomes. Considering the invasive potential of giant miscanthus, several POST herbicides evaluated in this study such as fluazifop, pyrithiobac, and sulfometuron may be viable options to control this species if it becomes invasive.

Injury risk factors in elite Australian football

Introduction: Injury prevention and individual player management are increasingly prioritised as effective strategies tominimise injury time loss in professional team sport. Modifications to training practices are in part dependent on the identification of risk factors associated with injury. Despite suggestions that various components of physical fitness may either protect or predispose a player to injury, the links between physical fitness and injury risk in Australian football are relatively unexplored. The purpose of this study was to assess the relationships between individual player characteristics assessed in the pre-season and injury in elite AFL football. Methods: Player characteristics (height, mass, age, experience, playing position) including pre-season fitness (6min run, 40m sprint, 6m×40m sprint, vertical jump), and in-season injury data were collected over four AFL seasons. Data were analysed for 69 players from a single club, for a total of 3879 player rounds and 174 seasons. Injury risk (odds ratio) and injury impact (matchesmissed; rate ratio)were assessedusing a series ofmulti-level univariate and multivariate hierarchical linear models. Results: A total of 177 injuries were recorded over the four years with 494matchesmissed as a result (2.8±3.3matches/injury). The vast majority (87%) of injuries were lower body in nature, with hamstring (20%) and groin/hip (14%) being most prevalent. Nineteen players (28%) suffered a recurrent injury (34 instances, 19%). Injury risk and impact was significantly increased in players with lower body mass (p<0.01), lower 6min run performance (p<0.05) and playing as a forward (p<0.05). Increased height was associated with greater injury impact (p<0.01). There was a tendency (p<0.1) for slower players over 40m to have an increased risk of injury and impact. Univariate analysis suggested that higher vertical jump contact time reduced injury risk and impact, however this relationship was not supported when other variables were controlled. Age and previous AFL experience were not important predictors of injury in this playing group. Conclusion: The identification of injury risk factors that are intrinsic and modifiable is important in individual player management, while the potential to modify these risk factors is greatest in the pre-season period. Aerobic running fitness and increased bodymass appear to have a protective effect against injury risk and impact in elite AFL football and represent important characteristics for targeted development in susceptible players.

INCIDENCE OF LETTUCE TIPBURN AS RELATED TO HYDROPONIC SYSTEM AND CULTIVAR

The incidence of tipburn was studied by growing late winter and spring lettuce cultivars in two consecutive experiments, in hydroponics, either with Deep Flow Technique (DFT) or on solid growth substrates, in Messinia, NW, Greece. Both experiments showed that the fresh marketable yield of lettuce in DFT was approximately twice as great compared to that of plants grown on solid substrates. Tipburn injury was not observed in the late winter experiment whereas it was severe in the spring one. In particular, lettuce grown with DFT in the spring presented significantly greater level of injury whereas when grown on perlite the least. Their shoot calcium concentration was significantly lowest whereas when grown on perlite the highest. Between the two spring cultivars included, ‘Merlin’, which presented the greatest injury, also had significantly higher shoot fresh weight, lower root/shoot ratio, higher tissue water content, and lower nitrogen, potassium, calcium, and magnesium concentrations compared to ‘Gramsi’.

Response of Rice to Drift Rates of Glyphosate Applied at Low Carrier Volumes

Field studies were conducted near Crowley, LA in 2005 through 2007 to evaluate the effects of simulated herbicide drift on ‘Cocodrie' rice. Each application was made with the spray volume varying proportionally to herbicide dosage based on a constant spray volume of 234 L ha−1 and a glyphosate rate of 863 g ae ha−1. The 6.3%, 54–g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1, and the 12.5%, 108–g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. Compared with the nontreated, glyphosate applied at one tiller, panicle differentiation (PD), and boot resulted in increased crop injury. The greatest injury was observed on rice treated at the one-tiller timing. Applications of glyphosate at one tiller, PD, and boot reduced plant height at harvest and primary and total crop yield. Rice treated at primary crop maturity was not affected by glyphosate applications.

A zebrafish model of conditional targeted podocyte ablation and regeneration

Podocytes are specialized cells that contribute critically to the normal structure and function of the glomerular filtration barrier. Their depletion plays an important role in the pathogenesis of glomerulosclerosis. Here, we report generation of a genetic model of conditional podocyte ablation and regeneration in zebrafish using a bacterial nitroreductase strategy to convert a prodrug, Metronidazole, into a cytotoxic metabolite. A transgenic zebrafish line was generated that expresses a green fluorescence protein (GFP) and the nitroreductase fusion protein under the control of the podocin promoter Tg(podocin:nitroreductase-GFP). Treatment of these transgenic zebrafish with Metronidazole results in podocyte apoptosis, a loss of nephrin and podocin expression, foot process effacement, and a leaky glomerular filtration barrier. Following Metronidazole washout, proliferating cells were detected in the glomeruli of recovering transgenic fish with a restoration of nitroreductase-GFP fluorescence, nephrin and podocin expression, a reestablishment of normal foot process architecture and glomerular barrier function. Thus, our studies show that zebrafish podocytes are capable of regenerating following depletion and establish the Tg(podocin:NTR-GFP) fish as a new model to study podocyte injury and repair.

Access to the parapharyngeal space: An anatomical study comparing the endoscopic and open approaches

A subtemporal preauricular approach to the infratemporal fossa and parapharyngeal space has been the traditional path to tumors of this region. The morbidity associated with this procedure has lead to the pursuit of less invasive techniques. Endoscopic access using a minimally invasive transmaxillary/transpterygoid approach potentially may obviate the drawbacks associated with open surgery. The anatomy of the parapharyngeal space is complex and critical; therefore, a comparison of the anatomy exposed by these different approaches could aid in the decision making toward a minimally invasive surgical corridor. Technical Note. The parapharyngeal space was accessed endonasally by removal of the medial and posterior walls of the maxillary sinus. To allow better visualization and increased triangulation of a bimanual dissection technique, a sublabial canine fossa antrostomy was created. The medial and lateral pterygoid plates were removed. Further lateral dissection exposed the relevant anatomy of the parapharyngeal space. A subtemporal preauricular infratemporal approach was also completed. The endoscopic approach provided sufficient access to the superior portion of the parapharyngeal space. The open approach also provided adequate access; however, it required a larger surgical window, causing greater injury. A significant advantage of the subtemporal approach is the improved access to the petrous portion of the internal carotid artery. Conversely, the endonasal approach provided improved access to the anterior and medial portions of the superior parapharyngeal space. Endoscopic endonasal access utilizing a transmaxillary/transpterygoid approach provides a sufficient surgical window for tumor extirpation. Utilization of this approach obviates some of the morbidity associated with an open procedure. 5.

Eleutheroside B or E enhances learning and memory in experimentally aged rats.

Eleutheroside B or E, the main component of Acanthopanax, can relieve fatigue, enhance memory, and improve human cognition. Numerous studies have confirmed that high doses of acetylcholine significantly attenuate clinical symptoms and delay the progression of Alzheimer's disease. The present study replicated a rat model of aging induced by injecting quinolinic acid into the hippocampal CA1 region. These rats were intraperitoneally injected with low, medium and high doses of eleutheroside B or E (50, 100, 200 mg/kg), and rats injected with Huperzine A or PBS were used as controls. At 4 weeks after administration, behavioral tests showed that the escape latencies and errors in searching for the platform in a Morris water maze were dose-dependently reduced in rats treated with medium and high-dose eleutheroside B or E. Hematoxylin-eosin staining showed that the number of surviving hippocampal neurons was greater and pathological injury was milder in three eleutheroside B or E groups compared with model group. Hippocampal homogenates showed enhanced cholinesterase activity, and dose-dependent increases in acetylcholine content and decreases in choline content following eleutheroside B or E treatment, similar to those seen in the Huperzine A group. These findings indicate that eleutheroside B or E improves learning and memory in aged rats. These effects of eleutheroside B or E may be mediated by activation of cholinesterase or enhanced reuse of choline to accelerate the synthesis of acetylcholine in hippocampal neurons.

Response of cranberry and kidney beans to linuron

Field studies were conducted in 2009 and 2010 at the Huron Research Station, Exeter, Ontario and the University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the tolerance of four cultivars of cranberry bean (“Etna”, “Hooter”, “SVM Taylor”, and “Capri”) and four cultivars of kidney bean (“Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty”) to linuron applied preemergence at 1125 and 2250 g·ai·ha-1. One week after emergence (WAE), linuron applied PRE caused 0.4% to 1.2% injury in “Etna”, “Hooter”, “SVM Tayler”, and “Capri” cranberry bean and 3.1% to 3.6% injury in “Red Hawk”, “Pink Panther”, “Calmont”, and “Majesty” kidney bean. At 2 and 4 WAE, there was no difference in injury among the dry bean cultivars. Contrast comparing injury due to linuron in cranberry vs kidney bean cultivars indicated 2.3%, 1.7%, and 1.2% greater injury in kidney bean compared to cranberry bean at 1, 2, and 4 WAE, respectively. Linuron PRE caused slightly greater injury in kidney bean compared to cranberry bean but crop injury was minimal with no adverse effect on plant height, shoot dry weight, seed moisture content, and yield under the environments evaluated. Based on this research, linuron applied PRE at the proposed rate of 1125 g·ai·ha-1 can be safely used in cranberry and kidney beans in Ontario.

Efficacy of Insecticides against American Serpentine Leaf Miner, Liriomyza trifolii (Burgess) on Tomato Crop in N-W region of Uttar Pradesh, India

The American serpentine leaf miner, Liriomyza trifolii (Burgess) was observed as an important pest of tomato crop. Studies were conducted on the efficacy of selected insecticides against this pest. Larval mining cause greater injury. Among eight insecticides tested for their efficacy against L. trifolii on variety Avinash of tomato, Profenophos 40% + Cypermethrin 4% was found to be the most effective over control, while other insecticides were also showed significant result on the leaf miner infestation.

High Grade Renal Injuries: Application of Parkland Hospital Predictors of Intervention for Renal Hemorrhage

Investigators from Parkland Hospital proposed substratification of the AAST (American Association for the Surgery of Trauma) grading scale based on 3 risk factors, including active vascular extravasation, a medial laceration and a perinephric hematoma of greater than 3.5 cm. We hypothesized that these characteristics would also be associated with intervention for renal hemorrhage in our large trauma series. From January 2005 to January 2011 we retrospectively reviewed the renal trauma records at adult level 1 trauma centers in Utah. AAST grade 3 and 4 injuries were characterized based on the mentioned 3 risk factors. Our primary outcome was intervention to control renal hemorrhage. AAST grade 3 or greater injury was identified in 147 patients, including 115 who had grade 3 and 4 injuries as well as imaging available for review. There were 63 grade 3 (53%) and 52 grade 4 (43%) renal injuries. Eight patients (7%) underwent intervention for renal hemorrhage. Vascular extravasation (OR 16.4, 95% CI 2.6-179.8, p <0.001) and perinephric hematoma greater than 3.5 cm (OR 8.4, 95% CI 1.4-52.5, p = 0.0099) were associated with intervention, while a medial laceration was not (p = 0.454). Patients with 1 or fewer, 2 and 3 risk factors had an intervention rate of less than 2.9%, 18% and 50%, respectively (p <0.001). Vascular extravasation, a perinephric hematoma greater than 3.5 cm and the number of risk factors (0 to 3) were associated with intervention for renal hemorrhage. Our findings are similar to those at Parkland Hospital. These imaging features may serve as useful prognostic indicators for renal trauma.

Comparison of the Herbicidal Activity of Phenyl Isothiocyanate with Methyl Bromide in Polyethylene-Mulched Tomato

Field experiments were conducted in 2006 and 2007 to evaluate the herbicidal activity of phenyl isothiocyanate (ITC) on yellow nutsedge, Palmer amaranth, and large crabgrass in tomato grown on two polyethylene-mulched types. Treatments included two mulch types (low density polyethylene [LDPE] mulch and virtually impermeable film [VIF] mulch) and phenyl ITC at 0, 15, 75, 150, 750, and 1,500 kg ha−1. A standard rate of methyl bromide/chloropicrin (67 : 33%) at 390 kg ha−1under LDPE mulch was included for comparison. Regardless of mulch type, phenyl ITC at 1,452 (±133) and 1,719 (±426) kg ha−1was required for broad-spectrum weed control equivalent to methyl bromide in 2006 and 2007, respectively. Tomato injury was ≥ 44% at the highest phenyl ITC rate of 1,500 kg ha−1at 2 wk after transplanting (WATP) both years, irrespective of mulch type. Greater crop injury was observed from 750 kg ha−1of phenyl ITC in 2006 (≥ 27%) than in 2007 (≤ 10%). The greater injury in 2006 was attributed to a higher phenyl ITC concentration because holes in the plastic mulch for transplanting were punched at the time of transplanting in 2006; whereas, in 2007 holes were punched 2 d before transplanting, allowing 2 d of aeration before transplanting. Tomato marketable yield at all rates of phenyl ITC was lower than with methyl bromide in 2006. However, in 2007, marketable yield in plots treated with phenyl ITC at 750 kg ha−1was equivalent to methyl bromide. Overall, VIF mulch was no more effective than LDPE mulch at increasing weed control or improving the marketable yield of tomato either year.

Intra-parenchymal ferrous iron infusion causes neuronal atrophy, cell death and progressive tissue loss: Implications for intracerebral hemorrhage

Intracerebral hemorrhage (ICH) is a devastating stroke causing considerable tissue destruction from mechanical trauma and secondary degeneration. Free iron, released over days from degrading erythrocytes, causes free radicals that likely contribute to delayed injury. Indeed, an intracerebral injection of iron rapidly kills cells and causes cerebral edema. We expanded upon these observations by: determining a dose–response relationship of iron infusion, examining the structural appearance of surviving striatal neurons, and evaluating injury over months. First, we measured 24-h edema in rats given 3.8, 19.0 or 38.0μg infusions of FeCl2 (i.e., 30μL of a 1, 5 or 10mmol/L solution). Second, rats were given these infusions (vs. saline controls) followed by behavioral assessment and histology at 7days. Third, dendritic structure was measured in Golgi–Cox stained neurons at 7days after a 0.95-μg dose (30μL of a 0.25mmol/L solution). Last, rats survived 7 or 60days post-injection (19.0μg) for histological assessment. Larger doses of iron caused greater injury, but this was generally not reflected in behavior that indicated similar deficits among the 3.8–38.0μg groups. Similarly, edema occurred but was not linearly related to dose. Even after a low iron dose the surviving neurons in the peri-injury zone were considerably atrophied (vs. contralateral side and controls). Finally, continuing tissue loss occurred over weeks with prominent neuronal death and iron-positive cells (e.g., macrophages) at 60days. Iron alone may account for the chronic degeneration found after ICH in rodent models.

Resistência do girassol a herbicidas inibidores da enzima acetolactato sintase

O girassol é bastante sensível a herbicidas aplicados em pós-emergência da cultura, com o objetivo de controlar espécies daninhas de folhas largas. Diante disto, foram desenvolvidos genótipos resistentes a herbicidas do grupo químico das imidazolinonas. Este trabalho objetivou avaliar a seletividade de herbicidas dos grupos químicos das imidazolinonas e sulfonilureias, aplicados sobre plantas de girassol (Tera 8003 e Tera 8011) resistentes aos inibidores da enzima acetolactato sintase (ALS). Experimentos foram conduzidos em área experimental da Embrapa Gado de Leite, nos municípios de Coronel Pacheco (MG) e Valença (RJ). O delineamento experimental foi em blocos casualizados, com quatro repetições. Os tratamentos foram: testemunha capinada, imazapyr 25 g i.a. ha-1 e 50 g i.a. ha-1, imazethapyr 70 g i.a. ha-1 e 100 g i.a. ha-1, nicosulfuron 20 g i.a. ha-1 e 32 g i.a. ha-1 e chlorimuron 7,5 g i.a. ha-1 + 0,05% v/v de óleo mineral. Foi avaliada a percentagem de fitotoxicidade, teor de clorofila (índice SPAD), altura de plantas, produção e percentagem de matéria seca e produtividade. As doses de 70 g i.a. ha-1 e 100 g i.a. ha-1 de imazethapyr foram as mais seletivas, a dose de 20 g i.a. ha-1 do nicosulfuron apresentou tolerância moderada e os tratamentos com imazapyr e chlorimuron foram aqueles que causaram maior injúria, para ambos os híbridos de girassol.

Tillage Affects Imazamox Carryover in Yellow Mustard

Farmers grow crops in the dryland region of the Pacific Northwest (PNW) using tillage practices ranging from moldboard plowing to no-tillage. The objective of this study was to determine the effect of tillage on persistence of imazamox herbicide in intermediate and high precipitation zones of the inland PNW. Along with a nontreated control, imazamox was applied to imidazolinone-tolerant winter wheat in the fall and spring at one, two, and three times the maximum labeled rate at locations near Genesee, ID, Davenport, WA, and Pendleton, OR. Moldboard plow, chisel plow, and no-till tillage treatments were implemented soon after wheat harvest and yellow mustard was planted the following season to determine crop response. Experiments were conducted at each location in 2005 to 2007 and 2006 to 2008. There were significant location by year and year and location interactions. There was no significant tillage by imazamox rate interaction, except at Pendleton in year 2, for all measured yellow mustard responses (crop injury, biomass, and yield). Genesee was colder than Pendleton and had more precipitation than Davenport, resulting in more injury to yellow mustard at Genesee than at Pendleton but less than at Davenport. Davenport had greater injury than the other two locations, likely due to lower soil pH, higher organic matter (OM), and cooler, drier climate, which allowed imazamox to persist longer in the soil. Overall, Pendleton had the least yellow mustard injury, which likely was related to its warmer, wetter climate and the concomitant rapid soil dissipation of imazamox. Tillage did not reduce the persistence of imazamox. Yellow mustard had the lowest injury and had greater mature biomass and seed yield in no-till seeded plots when averaged across imazamox rates compared to moldboard and chisel-plowed plots.

BEARD-PULLING AND FURNITURE-REARRANGING: Conflict Within the Seventeenth-Century Audiencia of Santo Domingo

On April 8,1626, a violent confrontation took place between the president and one of the oidores of the audiencia of Santo Domingo. According to a letter from oidor Alonso de Cereceda, President Diego de Acuña had called three of the magistrates and the fiscal of the audiencia to his residence in order to question them in private about their response to a message from Acuña's wife, doña Ana de Acuña, instructing them to release a prisoner. When Cereceda suggested that doña Ana's involvement in official business was undermining the tribunal's authority, Acuña flew into a rage and physically assaulted the oidor, punching him in the body and face and knocking his chair to the ground. After the other two oidores and the fiscal pulled the two men apart, Acuña called some of the presidio soldiers into the chamber to guard the letrado officials with drawn swords. Cereceda claimed that while these other witnesses were present, Acuña “attacked me two or three more times, throwing punches at me and putting his hand to my beard for greater injury.” He was then escorted home by the sargento mayor of the presidio, while the other officials remained to calm the president. Following this altercation, Cereceda stopped attending the court's audiencia publica and acuerdo sessions in person, sending his votes with the audiencia scribes instead, until Acuña finally had him placed under arrest.

Co-application of herbicides and insecticides in dry bean

Eight field trials were conducted from 2006 to 2008 at various locations in Ontario to evaluate the co-application of postemergence herbicides with cyhalothrin-lambda or dimethoate insecticides in cranberry and white bean. At 2 weeks after treatment, the addition of cyhalothrin-lambda or dimethoate insecticides to sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen did not increase injury at the Exeter and Ridgetown locations except for bentazon plus dimethoate which caused greater injury than bentazon alone (2.9% vs 0.2%) in 2006. However at Harrow, the addition of dimethoate to quizalofop-p-ethyl increased injury (0% vs 4.9%) in 2007 and the addition cyhalothrin-lambda or dimethoate to sethoxydim increased injury in 2008 in dry bean. The addition of cyhalothrin-lambda to quizalofop-p-ethyl also increased injury (0% vs 4.5%) in 2008. There was no adverse effect on dry bean injury with other treatments at Harrow in 2007 or 2008. The addition of cyhalothrin-lambda or dimethoate to the herbicides evaluated did not have any adverse effect on plant height, shoot dry weight or yield of dry bean except for bentazon plus dimethoate which decreased shoot dry weight 20% compared to bentazon alone at Harrow in 2008. Based on these results, cyhalothrin-lambda or dimethoate can be tank-mixed with sethoxydim, quizalofop-p-ethyl, bentazon, fomesafen and bentazon plus fomesafen when the optimum application timing of these herbicides and insecticides coincide.