Great Food Research Articles

Aptamer-based sensitive fluorescence β-lactoglobulin food allergen bioassay via dual and cyclic bidirectional strand displacement amplifications.

β-Lactoglobulin (β-Lg) is a prevalent allergenic protein found in most dairy products, which poses great food safety risks for individuals with allergies, especially for infants. Sensitive and effective detection methods for such allergens are essential to reduce the risk of allergies in daily life. Herein, a fluorescent aptamer bioassay based on a dual and cyclic bidirectional strand displacement means is developed for thesensitive detection of β-Lg in infant rice porridge and milk. The aptamer in the duplex DNA probe binds β-Lg to release the assistance strand to further hybridize with two hairpins, which triggers the initiation of two cyclic amplification cycles through the polymerization, displacement, and nicking of the strands under the action of DNA polymerase and endonuclease restriction enzymes. The amplification cycles lead to the unfolding of many fluorescently quenched signal probes to exhibit substantially enhanced fluorescence recovery for detecting β-Lg. The assay can realize detection of β-Lg in concentrations as low as 4.41pM within the range of 0.01 to 10nM. Furthermore, our sensing method has the capability to discriminate β-Lg from other proteins with high selectivity, resulting in low levels of β-Lg detection in rice porridge and milk samples, demonstrating promising potentials of the developed sensing method for monitoring various food allergens.

Canonical correlations between wheat traits in multienvironment trials

ABSTRACT Wheat is a crop of great food importance for food production in Brazil. However, the improvement of this crop is still concentrated in isolated areas, which hinders advances in new cultivation regions due to the extensive demand for existing information. In this investigation, four wheat genotypes analysed to study associations and interrelationships between wheat traits across nine environments during the agricultural years of 2016, 2017 and 2018. The experiment was conducted using a randomised block design with four repetitions, and the analysis was divided into two groups: morphological and productive traits of wheat, comprising three and eleven characteristics, respectively. Canonical correlations between the morphological and productive groups were found to be dependent in most environments. Taller plants with longer main spikes and longer tiller spikes can be indirectly selected to increase grain yield under different sowing conditions. The information generated in this study indicate that some care must be taken when using the interrelations between trait groups to select or recommend genotypes for broader regions, as these relationships are influenced by the specific conditions of each region and sub-region.

A tribute to Arne Schousboe's contributions to neurochemistry and his innovative and enduring research in GABA, glutamate, and brain energy metabolism.

This is a tribute to Arne Schousboe, Professor Emeritus at the University of Copenhagen, an eminent neurochemist and neuroscientist who was a leader in the fields of GABA, glutamate, and brain energy metabolism. Arne was known for his keen intellect, his wide-ranging expertise in neurochemistry and neuropharmacology of GABA and glutamate and brain energy metabolism. Arne was also known for his strong leadership, his warm and engaging personality and his enjoyment of fine wine and great food shared with friends, family, and colleagues. Sadly, Arne passed away on February 27, 2024, after a short illness. He is survived by his wife Inger Schousboe, his two children, and three wonderful grandchildren. His death is a tremendous loss to the neuroscience community. He will be greatly missed by his friends, family, and colleagues. Some of the highlights of Arne's career are described in this tribute.

The Flavonoid Glycoside from Abrus cantoniensis Hance Alleviates Alcoholic Liver Injury by Inhibiting Ferroptosis in an AMPK-Dependent Manner.

Abrus cantoniensis Hance is a vegetative food and can be used as a folk beverage or soup to clear liver toxins and prevent liver damage. However, the components and effects of A. cantoniensis Hance in alcohol-induced liver injury were unknown. This study aimed to obtain abundant phytochemicals from A. cantoniensis Hance and identify the potency of the isolates in preventing alcohol-induced liver injury. Alcohol-stimulated AML12 cells and Lieber-DeCarli diet-fed mice were used to establish in vitro and in vivo models, respectively. Our findings indicated that flavonoid glycosides, especially AH-15, could significantly alleviate alcohol-induced liver injury by inhibiting oxidative stress. Furthermore, we demonstrated that AH-15 inhibited ferroptosis induced by lipid peroxidation. Mechanically, we found that AH-15 regulated nuclear factor erythroid 2-related factor 2 (NRF2) expression via activation of AMP-activated protein kinase (AMPK) signaling. These results indicate that A. cantoniensis Hance is a great potential functional food for alleviating alcohol-induced liver injury.

Focusing on New Discoveries in Food Technology and Creating a New Future of Nutrition and Health: An Introduction to the 4th International Symposium of Food Science, Nutrition and Health in Dalian, China.

The 4th International Symposium on Food Science, Nutrition and Health (ISFSNH) was held at the Shangri-La Hotel in Dalian, China, on May 29-31, 2023. The symposium explored the connotations and needs of "The Great Food Perspective" under the theme "Focusing on new discoveries in food technology and creating a new future of nutrition and health" to better address the global emerging diverse food needs. The ISFSNH covered four areas: (1) food processing theory and technology, (2) food safety and quality control, (3) precision nutrition and health, and (4) creation of nutritious and healthy foods. More than 1000 scholars and entrepreneurs from more than 100 colleges and universities globally attended the conference. This special issue of the Journal of Agricultural and Food Chemistry highlights the important topics of the 4th ISFSNH and includes more than 20 papers.

Influence of the processing on composition, protein structure and techno-functional properties of mealworm protein concentrates produced by isoelectric precipitation and ultrafiltration/diafiltration

Edible insects represent a great alternative protein source but food neophobia remains the main barrier to consumption. However, the incorporation of insects as protein-rich ingredients, such as protein concentrates, could increase acceptance. In this study, two methods, isoelectric precipitation and ultrafiltration-diafiltration, were applied to produce mealworm protein concentrates, which were compared in terms of composition, protein structure and techno-functional properties. The results showed that the protein content of the isoelectric precipitation concentrate was higher than ultrafiltration-diafiltration (80 versus 72%) but ash (1.91 versus 3.82%) and soluble sugar (1.43 versus 8.22%) contents were lower. Moreover, the protein structure was affected by the processing method, where the ultrafiltration-diafiltration concentrate exhibited a higher surface hydrophobicity (493.5 versus 106.78 a.u) and a lower denaturation temperature (161.32 versus 181.44 °C). Finally, the ultrafiltration-diafiltration concentrate exhibited higher solubility (87 versus 41%) and emulsifying properties at pH 7 compared to the concentrate obtained by isoelectric precipitation.

Phenotypic Diversity of Quinoa Landraces Cultivated in the Ecuadorian Andean Region: In Situ Conservation and Detection of Promising Accessions for Breeding Programs

Quinoa (Chenopodium quinoa Willd.) is an ancestral crop in the Ecuadorian Andean region, and its landraces have always been of great social and food importance for the native population. Currently, there is no updated information about their phenotypic diversity and conservation status nor about the changes that have occurred in the last decades. A total of 268 accessions of quinoa landraces collected at two different times (1978–1988 and 2014–2015) in three representative Ecuadorian Andean provinces (Imbabura, Cotopaxi and Chimborazo) were evaluated for forty agro-morphological (17 quantitative and 23 qualitative) traits. Most of the quantitative traits showed high variability, some of them with great importance for commercialization and germplasm selection for breeding programs (e.g., panicle width, grain width, 1000-grain weight or seed yield per plant). Ten quantitative and eleven qualitative descriptors were significantly different between both collections. Regarding the presence/absence of saponin, all the accessions collected four decades ago had saponin, while it was found in only 18% of accessions collected more recently. The phenotypic relationships in the dendrogram did not show clustered accessions by their geographical origin or by collection. A selection index allowed us to detect a few accessions recently collected in Chimborazo with high promises for future breeding programs, with high seed yields per plant values and a reduced or no saponin content. The agro-morphological information obtained may be very useful for the suitable management and conservation of this ancestral plant genetic resource, both on the farm by indigenous farming communities and ex situ by the Germplasm Bank of the Ecuadorian National Institute for Agricultural Research (INIAP).

Possible Outbreak of Newcastle Virus Infection in the Face of Increasing Trend of Backyard Poultry Farm Management Interface in Nigeria: A Public Health and Food Security Threat

Introduction: Newcastle disease is a highly contagious illness and one of the "notifiable illnesses" on the World Health Organization list for Animal Health (OIE) Outcome. There are constantly new cases being reported as new NDV isolates from around the world. Newcastle disease poses a significant global economic threat to the poultry sector, causing highly contagious and transmissible diseases in birds with huge food security threat among the weak and vulnerable developing and underdeveloped countries Aim: The aim of this article borders on bringing to light the risk of possible Newcastle disease outbreak in Nigeria due to increasing poor management of poultry farms and poultry waste product. Furthermore, it will also underscore and outline various ways which can be adopted to prevent this poultry disease which is of great public health, economic and food security protection importance. Method/Methodology: This is a systematic review of published research articles focusing on Newcastle disease, epidemiology, pathogenicity, transmission and factors that can play important roles in the possible outbreak of the disease in poultries. Studies reviewed comprise of cross-sectional, prospective, longitudinal and observational studies. The study approach follows the systematic review of peer-reviewed published articles as well as online publications and articles derived from various databases with search keywords relevant to the topic of discourse. Findings and Results: The findings in this study highlight the endemicity of Newcastle virus disease in various part of the country, and how the severity of the disease is influenced by host characteristics, viral properties, and environmental factors. The study pointed out critical evidence-based possible reasons that could promote a new round of Newcastle disease epidemic outbreak as a result of poor poultry management practices such as high bird densities, poor poultry infrastructure, lack of quarantine of sick birds, mixed species rearing, free range system of poultry, poor waste disposal, lack of expertise, and insufficient biosecurity outcomes. However, the lack of education and awareness among farmers exacerbates the problem, hindering preventive measures. The complexity is increased by difficulties with vaccination, unrestrained bird movement through trading with neighboring countries and interstate, and a lack of diagnostic facilities. The consequences of an outbreak extends beyond financial losses to potential disruptions in the poultry supply chain, food security risks, and public health concerns. Conclusion and Recommendation: The growing difficulties in poultry management methods increases the serious public health concern posed by a possible Newcastle Virus epidemic outbreak in Nigeria. A paradigm shift in poultry farming practices is necessary to address this problem. A comprehensive strategy that incorporates infrastructural development, regulatory frameworks, and education in order to strengthen the poultry industry is needed, so as to address and prevent the looming public health and food security crises that may break out from the above scenario

Perfil do consumo de tuberosas amiláceas no Estado de São Paulo e seus produtos processados

Starchy tubers are of great food importance in developed and developing countries, whether for food security, cultural habits or industrialization. São Paulo stands out in the production of some of these vegetables, which together move a large volume of products in different channels. Thus, the study aimed to trace the consumption profile of these vegetables by the population. For this, a digital quiz was carried out to 561 people from different locations. Potatoes and cassava are the vegetables consumed by the largest number of people, and potatoes are the most frequently consumed. For most people, these vegetables are present in their diet on a weekly basis, and the main reason for choosing the product is the consumer preference. Supermarkets/greengrocer/retailers are the main purchasing channels. Straw potatoes are the most frequent processed food in the usual purchases, however processed products made from cassava are purchased in a greater variety of items.

Effects Caused by the Ingestion of Microplastics: First Evidence in the Lambari Rosa (Astyanax altiparanae).

Microplastics are a class of contaminants that pose a threat to aquatic biota, as they are easily found in aquatic ecosystems and can be ingested by a wide variety of organisms, such as fish. The lambari rosa (Astyanax altiparanae) is a microphage fish, which feeds on microscopic beings and particles, making it potentially susceptible to ingesting MPs discarded in the environment. In addition, this fish is of great economic and food importance, as it is used for human consumption. This study aimed to evaluate the accumulation and possible toxicological effects caused to lambari rosa (n = 450) by the ingestion of polyethylene (PE) and polyethylene terephthalate (PET) MPs, since the MPs of these polymers in the form of granules, fragments, and fibers are the most commonly reported in the aquatic environment. The parameters investigated here were the quantitative analysis of ingested MPs using microscopic and staining techniques, as well as the mortality rate, malformations/injuries, and impaired weight gain. At the end of the experiment, it was concluded that MPs from both polymers accumulated in the gastrointestinal tract of the lambari rosa, and that dietary exposure, especially to the PET polymer, was responsible for increasing the mortality rate in this species.

“Great food, but the noise?”: Relationship between perceived sound quality survey and non acoustical factors in one hotel restaurant in Italy

The empirical assessment of the quality of the sound landscape in restaurants and how it affects the overall customer experience has only recently begun to be addressed. Noise from recreational activities, such as discos, restaurants, bars, hotels, etc., characterizes the soundscape of tourist centers, where there is a need to ensure the coexistence of noisy commercial activities and the right to rest of citizens customers of accommodation facilities, often already compromised by traffic. Although there is a renewed sensitivity to the issue of noise, restaurants are often characterized by poor acoustics and a poor multi-sensory perception of comfort. This article examines the noise levels of a hotel’s dining room in an Italian tourist resort during lunch and dinner hours. The study included aspects related to the acoustics, lighting, and quality of the location, also for the assessment of the perception of comfort obtained was given to customers a questionnaire of satisfaction during the meal, every day for a week. Considering the correlation between the three components of the soundscape, namely people, sound, and the environment under consideration, this study aims to explore the effects of the soundscape on sound restoration for accommodation facilities in tourist resorts.

Macro and trace element compositions and physicochemical parameters of guajiru fruits (Chrysobalanus icaco l.) from two Brazilian states

Chrysobalanus icaco L. is a tree found in America and Africa. In Brazil it can be found in the biome along the coast. It has great food potential and its fruits, named guajiru, are consumed by fishing communities in natura and processed as jams and jellies. However, the literature on this tree species is limited. This study aims to describe the element contents and physicochemical characteristics of the C. icaco fruits from two regions in Brazil and investigate if they influence its sensory characteristics. The fruits were collected in the Northeastern region of Brazil in Maracajaú, State of Rio Grande do Norte (RN) and in Salvador, Bahia (BA). The morphometric characteristics, color, texture, and sensory analyses were carried out on the fruits in natura, and the physicochemical analyses and mineral profile on the pulp homogenized with the peel. The mineral profile showed K > Na > Mn > Mg > Ca > Fe > Mn > Cu > Zn > Ni > Cr, varying from 206 mg/100g (K) to 0.87 μg/100 g (Cr). The fruits from RN presented higher levels of trace and macronutrients, which affected the sensory characteristics, mainly the flavor, and led to better acceptance of these samples as compared to those from BA. The results suggest that C. icaco fruits present a good nutritional profile and sensory acceptance and hence their commercialization could be stimulated as a table fruit or mixed with other fruits.

Determinants of Consumption Structure of Livestock Products among Rural Chinese Residents: Household Characteristics and Regional Heterogeneity

Livestock products provide essential micro-nutrients and animal protein to rural residents. Upgrading the consumption pattern of livestock products is not only crucial for improving rural residents’ health and quality of life but also for achieving food security and a healthy China under the “Great Food View” development concept. To identify the characteristics and determinants of residents’ livestock products consumption structure in rural China, we used the Logit model to empirically analyze the key factors affecting rural residents’ consumption structure of livestock products from the perspective of household characteristics and regional heterogeneity using a survey data of 4529 rural residents across 10 provinces. The findings reveal that, firstly, rural residents consume a variety of livestock product categories, predominantly meat, meat-egg, meat-milk, and meat-egg-milk. Secondly, the family characteristics of the number of people eating at home, education level, access to food nutrition and health information, ethnicity, and age structure significantly affect the consumption structure of livestock products among rural residents. Thirdly, self-raising of livestock and poultry can support rural residents’ consumption of livestock products. Fourthly, there are notable variations in the consumption structure of livestock products between different regions. Based on this, we suggest strategies to optimize and support the consumption structure of livestock products for rural residents, such as promoting consumption upgrading, advocating market segmentation for livestock products, and improving regional self-sufficiency.

Research on the Construction of China’s Provincial Food Security Evaluation System and Regional Performance—Based on the “Great Food View”

Based on the internationally recognized concept of food security and the scientific connotation of the “Great Food View”. This research constructs China’s provincial food security evaluation system under the “Great Food View” by comprehensively considering the regional food supply logic and dietary habits differences. Combining the improved entropy weight method and AHP to quantitatively evaluate the current situation and trend of food security in China’s provinces (except Hong Kong, Macao, and Taiwan) from 2009 to 2021 by using this system—on the basis of dividing China’s provinces into main food production provinces and non-main food production provinces. The results demonstrate that all provinces have entered the ranks of “security” and above food security, and the mismatch between “high vulnerability and low security” has been alleviated. Yet, the problem of “difficult growth” in the provinces with the lowest score of food security has emerged. The green and sustainability of the food security system in the main food production areas have been at a low level, and the food security in non-main food production areas has seen the “Matthew effect” of uneven development. Finally, policy implications are proposed from the aspects of diversified development of food sources, precision agriculture subsidy guide, optimization of farmland compensation and protection policies, and optimization of food storage and distribution.

Delivery of natural Monascus yellow pigment using zein-lecithin nanoparticles: Fabrication, characterization, and in vitro release properties

Monascus yellow pigment (MYP) is an important secondary metabolite of polyketone produced by Monascus sp. MYP is not only a great food colorant but also possesses beneficial active functions for human body. However, its application is currently limited due to the pH instability and low bioavailability. This can be solved by loading MYP in zein-lecithin nanoparticles. The structure and encapsulation efficiency of nanoparticles varied due to the different proportions of zein-lecithin-MYP components. When the mass ratio was 4:4:1, the encapsulation efficiency was 60.82% and loading capacity was 12.61%, respectively. Scanning electron microscope observation showed that zein-lecithin-MYP nanoparticles (MYP@Z-L NPs) were globular and uniformly distributed with increasing of lecithin content. The results of Fourier infrared spectroscopy confirmed that MYP was successfully embedded in the nanoparticles, primarily through hydrogen bonding, electrostatic and hydrophobic interactions. Stability study of MYP@Z-L NPs showed that it had better resistance to low pH than free MYP and could be stored for up to 4 weeks. Therefore, the zein-lecithin composite nanoparticles with a mass ratio of 4:1 can be an effective carrier for the active protection and long-term utilization of MYP and have a great application potential in the food industry.

Form-stable cold storage phase change materials with durable cold insulation for cold chain logistics of food

Every year, many foods are discarded in the process of transportation because they could not be kept fresh for a long time, resulting in great food and economic loss. Phase change technology for cold energy storage has the advantages of stable temperature and high energy storage density, and it has great application potential in cold chain logistics. However, how to endow it with shape stability before and after phase change and machinability while maintaining high cold-insulation performance is of great importance for expanding its application in the fields of cold chain logistics with higher requirements. Herein, a novel form-stable cold energy storage phase change material (FCPCM) with ice as phase change component and a polyether-based three-dimensional network as skeleton was reported. FCPCMs exhibit excellent shape stability, i.e., no water leakage, owing to the coaction of capillary forces and hydrogen bonding between two phase interfaces. FCPCM demonstrated excellent thermal properties (ΔHm = 285.9 J/g and Tp of melting = 1.9 °C) and durable cold-insulation performance, and it could maintain cold temperature 1.85 times longer than ice. FCPCM could prolong the preservation time of litchi, indicating its excellent potential in cold chain logistics of fruit, vegetables, and other foods to enhance food quality and safety.

Identification of Single-Nucleotide Polymorphisms (SNPs) Associated with Heat Tolerance at the Reproductive Stage in Synthetic Hexaploid Wheats Using GWAS.

The projected rise in global ambient temperature by 3-5 °C by the end of this century, along with unpredicted heat waves during critical crop growth stages, can drastically reduce grain yield and will pose a great food security challenge. It is therefore important to identify wheat genetic resources able to withstand high temperatures, discover genes underpinning resilience to higher temperatures, and deploy such genetic resources in wheat breeding to develop heat-tolerant cultivars. In this study, 180 accessions of synthetic hexaploid wheats (SHWs) were evaluated under normal and late wheat growing seasons (to expose them to higher temperatures) at three locations (Islamabad, Bahawalpur, and Tando Jam), and data were collected on 11 morphological and yield-related traits. The diversity panel was genotyped with a 50 K SNP array to conduct genome-wide association studies (GWASs) for heat tolerance in SHW. A known heat-tolerance locus, TaHST1, was profiled to identify different haplotypes of this locus in SHWs and their association with grain yield and related traits in SHWs. There was a 36% decrease in grain yield (GY), a 23% decrease in thousand-grain weight (TKW), and an 18% decrease in grains per spike (GpS) across three locations in the population due to the heat stress conditions. GWASs identified 143 quantitative trait nucleotides (QTNs) distributed over all 21 chromosomes in the SHWs. Out of these, 52 QTNs were associated with morphological and yield-related traits under heat stress, while 15 of them were pleiotropically associated with multiple traits. The heat shock protein (HSP) framework of the wheat genome was then aligned with the QTNs identified in this study. Seventeen QTNs were in proximity to HSPs on chr2B, chr3D, chr5A, chr5B, chr6D, and chr7D. It is likely that QTNs on the D genome and those in proximity to HSPs may carry novel alleles for heat-tolerance genes. The analysis of TaHST1 indicated that 15 haplotypes were present in the SHWs for this locus, while hap1 showed the highest frequency of 25% (33 SHWs). These haplotypes were significantly associated with yield-related traits in the SHWs. New alleles associated with yield-related traits in SHWs could be an excellent reservoir for breeding deployment.

Evaluation of the Potential of Duckweed as a Human Food, Bioethanol Production Feedstock, and Antileukaemia Drug

This study evaluated the potential of duckweed as a human food, ethanol feedstock, and anticancer drug. First, the nutritional value of wild duckweed was reported for the first time. Its main composition was similar to that of artificially cultivated duckweed, and thus, wild duckweed can serve as a great human food source. In addition, high-starch duckweed induced by nutrient starvation was fermented into bioethanol. A yield of 0.262 g/g, the highest duckweed-ethanol yield reported thus far, was achieved, indicating that duckweed is an excellent feedstock for ethanol production. Finally, the anticancer effects of duckweed flavonoids (DFs) were assessed for the first time using acute myeloid leukaemia (AML) cells as models in vitro and in vivo. The results revealed that DFs possessed antileukaemia activity and were safe and effective for AML therapy. In conclusion, duckweed was demonstrated to be helpful for humans for food security, energy crisis remediation, and tumour treatment.

Fatty Acid Positions in Triacylglycerol of Iridescent Shark Fish Oil (Pangasius sp.), Focusing on Omega-3 and Omega-6 Fatty Acids.

<b>Background and Objective:</b> Considering the many health benefits of fish oil, the potential of Indonesian fisheries needs to be mapped to find local fish oil sources that have the opportunity to be used as a source of omega-3 and 6. This research aimed to ascertain the glyceride profile of iridescent shark fish oil hydrolyzed by immobilized lipase from <i>Thermomyces lanuginosus</i> at the sn-1,3 position and identify the position of omega-3 and omega-6 fatty acids. <b>Materials and Methods:</b> To extract the fish oil from the iridescent shark, the soxhletation method was utilized. The analysis of the fatty acid composition that was carried out using gas chromatography (GC) was previously esterified with BF<sub>3</sub> before it was carried out to position the fatty acid hydrolysis that was carried out using lipase enzymes to position the fatty acid composition. <b>Results:</b> The sample had more unsaturated fatty acids than saturated ones. Omega-3 and omega-6 fatty acids are more concentrated in the fat molecule's sn-2 position than in the sn-1+sn-3 location. Iridescent shark fish oil meets the recommended ratio of omega-3 to omega-6 (1:1) or better (2:1). <b>Conclusion:</b> It has been discovered that iridescent shark fish oil is rich in omega-3 and omega-6 fatty acids, especially those in the sn-2 position. This makes it a great food choice for those trying to get more omega-3 unsaturated fatty acids into their diets.

Incorporation of Poly(ethylene 2,5-furanoate) into Poly(butylene adipate-co-terephthalate) toward Sustainable Food Packaging Films with Enhanced Strength and Barrier Properties

The development of environment-friendly biodegradable materials has aroused widespread attention and interest. Herein, kilogram-scale poly(ethylene 2,5-furanoate) (PEF) was synthesized and incorporated into poly(butylene adipate-co-terephthalate) (PBAT) via melt-compounding. Then, the PBAT/PEF blend films were prepared by the extrusion blowing method. The results showed that PEF could greatly improve the tensile performance and tear resistance of PBAT/PEF blend films. The superior mechanical properties were achieved in PBAT/PEF10 blend films with a tensile strength of 44.3 ± 2.9 MPa and an elongation at break of 900.5 ± 21.9%. Besides, the transmittance of oxygen (O2), carbon dioxide (CO2), and water vapor sharply reduced by 78.8, 74, and 48.3% in comparison with those of a pure PBAT film, respectively. The inherent barrier characteristic of PEF and the enhanced bonding between PBAT and PEF contributed to the excellent gas and water vapor barrier performance. Furthermore, PBAT/PEF blend films were used for the fruit preservation tests. After a 15 day preservation, the freshness of fruits covered with PBAT/PEF films was well kept, showing great potentialin food packing applications.