In this paper, we study the newly discovered universal splitting behavior for tree-level scattering amplitudes of particles and strings [1]: when a set of Mandelstam variables (and Lorentz products involving polarizations for gluons/gravitons) vanish, the n-point amplitude factorizes as the product of two lower-point currents with n+3 external legs in total. We refer to any such subspace of the kinematic space of n massless momenta as “2-split kinematics”, where the scattering potential for string amplitudes and the corresponding scattering equations for particle amplitudes nicely split into two parts. Based on these, we provide a systematic and detailed study of the splitting behavior for essentially all ingredients which appear as integrands for open- and closed-string amplitudes as well as Cachazo-He-Yuan (CHY) formulas, including Parke-Taylor factors, correlators in superstring and bosonic string theories, and CHY integrands for a variety of amplitudes of scalars, gluons and gravitons. These results then immediately lead to the splitting behavior of string and particle amplitudes in a wide range of theories, including bi-adjoint ϕ3 (with string extension known as Z and J integrals), non-linear sigma model, Dirac-Born-Infeld, the special Galileon, etc., as well as Yang-Mills and Einstein gravity (with bosonic and superstring extensions). Our results imply and extend some other factorization behavior of tree amplitudes considered recently, including smooth splittings [2] and factorizations near zeros [3], to all these theories. A special case of splitting also yields soft theorems for gluons/gravitons as well as analogous soft behavior for Goldstone particles near their Adler zeros.