Gravimetric vapor sorption experiments were performed on beech wood samples to determine the directional permeability, diffusion and sorption coefficients in the three orthotropic wood directions. Dynamic Vapor Sorption (DVS) experiments allowed for the direct evaluation of the diffusion coefficient from the analysis of the kinetic sorption profile using a double stretched exponential model with values ranging from 0.10 × 10−10 to 1.52 × 10−10 m2/s and depending on the wood direction of the sample and the RH-values. Moisture sorption isotherms (MSIs) were constructed and fitted to a modified Guggenheim-Anderson-de Boer and a Sorption Site Occupancy model, which allowed for the calculation of the sorption coefficient which was found to be between 2.4 and 3.0 mol/(m3 Pa). Dynamic Vapor Transport (DVT) experiments were performed to calculate the permeability coefficient from the vapor flow rate and it ranges between 0.56 × 10−10 and 4.38 × 10−10 mol/(m s Pa) as a function of the flow direction and RH conditions. These results indicate that such an experimental approach is suitable for determining wood–moisture interactions.