Grass-clover Leys Research Articles

Similar root and stubble biomass carbon in grass–clover leys irrespective of yield, species composition, sward age, and fertilization

AbstractBackgroundCrop‐ and site‐specific quantification of non‐harvestable aboveground residues and root biomass is essential for predicting management‐induced changes in soil C storage.AimsThe aim of this study was to quantify stubble and root biomass C from productive grass–clover leys used for cutting as affected by fertilization and sward age.MethodsBased on an organic long‐term dairy crop rotations experiment with 4 years of grass–clover in a six‐course rotation, we examined the effects of fertilization (unfertilized and 300 kg total‐N ha−1 in cattle slurry) and sward age (1–4‐year‐old) on herbage yield and composition, stubble biomass, and composition and root biomass of grass–clover ley.ResultsLey duration and fertilization altered plant community composition and aboveground productivity but did not affect stubble and root biomass C.ConclusionsThe results question the use of yield‐dependent allometric functions for grass–clover ley used in simulation models and life cycle assessments for C accounting in agricultural systems. For predictions of soil C changes, we recommend the use of a fixed stubble‐derived C input from grass–clover ley regardless of sward age and fertilization‐induced differences in species composition, and herbage yield. Likewise, a fixed root‐derived C input for 1‐year‐old grass–clover, irrespective of fertilization, may be implemented. However, the contribution of continuous rhizodeposition and fine root turnover to root‐derived C input need to be accounted for.

Increasing cattle slurry application to grass-clover leys of different ages did not affect nitrate leaching but increased legacy effect in mixed organic crop rotations

ContextOrganic dairy farms with grass-clover often have a large N surplus, due to biological N fixation and in addition, manure application, which challenges the efforts to reduce N losses. Potential loss pathways include leaching from grass-clover ley and subsequent crops, but if managed appropriately this N legacy effect may result in higher yields in the subsequent crops. ObjectiveTo investigate the fate of the ley phase N surplus including losses of nitrate in grass-clover leys, contribution to the N supply of the subsequent crop, and the contribution from the long-term slurry N application to grass-clover leys to nitrate leaching and yields in subsequent crops. MethodsWe quantified nitrate leaching across three years in two six-year crop rotations with two or four years of grass-clover ley and examined legacy effect of the grass-clover ley in relation to short- or long-term N fertilization levels. This was done by measuring water nitrate contents below the root zone combined with water balance modelling. ResultsNitrate leaching in 1- to 4-yr-old grass-clover leys were not influenced by cattle slurry application of up to 300 kg total-N ha-1 (equivalent to approximately 170 kg available N). Leaching losses were 2 - 19 kg N ha-1 yr-1 across grassland ages and application rates. Arable crops showed correspondingly low nitrate leaching (4–24 kg N ha-1 yr-1) except for barley/peas (74 kg N ha-1 yr-1), where a catch crop was established after autumn mechanical weed control. The legacy effect of fertilization on N yield was found in the first year following cultivation of 2-yr-old grass-clover swards and in both year one and two following cultivation of 4-yr-old grass-clover swards. The legacy effect was generally better predicted by the long-term cumulated fertilizer N application during the last rotation (six years) as compared to the most recent grass-clover N application rate. Nitrate leaching in the arable phase of the mixed crop rotations was not affected by fertilizer application in the ley phase. However, this might depend on catch crop strategy in the arable crops. ConclusionsNitrate leaching in dairy crop rotations was not influenced by cattle slurry application. It was found that both the short- and long-term slurry application history were able to explain the legacy effect and of those, the long-term was the best predictor. ImplicationsThe study shows that N leaching reduction targets can be met in an organic dairy crop rotation when attention is paid to the full crop rotation and utilization of short- and long-term legacy effects of slurry application.

Marginal increase in nitrate leaching under grass–clover leys by slurry and mineral fertilizer

AbstractOn dairy farms, fertilization of grass-clover swards ensures stable grass yields but may increase the potential for nitrate leaching on light-textured soils. The aim of this study was to quantify the N use efficiency and nitrate leaching under fertilized grass-clover leys. The study was conducted over 2 years at two sites, with increasing applications of mineral fertilizer (0–480 kg available N ha−1) alone or in combination with a basic application of cattle slurry. For plots fertilized with mineral N, the N soil surface balance was independent of the application rate and in the same range as for unfertilized plots (− 11 to 51 kg N ha−1). However, when plots were fertilized with slurry N (+ mineral N), the surplus was substantially increased owing to the fraction of organic N applied in slurry (95–100 kg N ha−1) and higher biological N2 fixation inputs (55–228 kg N ha−1). The type of fertilizer had no effect on nitrate leaching across the full range of application rates. Nitrate leaching increased quadratically as a function of application rate, with a range of 3–117 kg N ha−1 (0.33–17 mg l−1 in soil solution sampled with suction cups) in the first year and less in the second year, when clover proportion was lower due to the self-regulatory nature of grass-clover mixtures. Importantly, the rate of marginal leaching increased with fertilization level: below 150 kg N ha−1 there was no additional leaching from fertilization and at 200 kg N ha−1 around 5% of additional fertilizer-N was leached. This is less than generally found for arable crops and thus even in intensive dairy systems, grass-clover leys are an environmentally favorable crop.

Epiphytic microbiota in Swedish grass-clover herbage and the effect of silage additives on fermentation profiles and bacterial community compositions of the resulting silages.

To investigate the epiphytic microbiota in grass-clover herbage harvested at different sites and occasions; and to explore the effect of different silage additives on the resulting silage microbiota. Herbage was harvested from grass-clover leys at geographically distributed sites in a long-term field experiment in Sweden, in early and late season of two consecutive years. Different silages were made from the herbage using; 1) no additive, 2) acid-treatment, and 3) inoculation by starter culture. Herbages were analyzed for botanical and chemical composition, and the resulting silages for products of fermentation. Bacterial DNA was extracted from herbage and silage samples, followed by sequencing using Illumina 16S rRNA amplicon sequencing. Herbage microbiota showed no clear correlation to site or harvesting time. Silage additives had a major effect on the ensiling process; inoculation resulted in well fermented silages comprising a homogenous microbiota dominated by the genera Lactobacillus and Pediococcus. A minor effect of harvest time was also observed, with generally a more diverse microbiota in second harvest silages. Untreated silages showed a higher relative abundance from non-lactic acid bacteria compared to acid treated silages. In most silages, only a few bacterial amplicon sequence variants contributed to most of the relative abundance. The epiphytic microbiota in grass-clover herbage were found to be random and not dependent on site. From a microbial point of view, the most predictable and preferable silage outcome was obtained by inoculation with a starter culture. Acid-treatment with formic- and propionic acid surprisingly resulted in a less preferable silage. Silage making without additives cannot be recommended based on our results.

Vertical rhizome disking to reduce Elymus repens (quackgrass) abundance in grass-clover leys

Elymus repens is a problematic perennial weed in annual crops, grasslands and leys. Rhizome fragmentation by vertical disking can potentially reduce E. repens abundance with minimal tillage, but data are lacking on its efficiency in forage production. In a two-year study (2017–2018, 2018–2019) conducted in two forage grass-clover leys that were mostly weed-free except for large E. repens populations, this study examined effects on forage yield, botanical composition, and E. repens rhizome biomass of rhizome fragmentation at significant growth initiation in spring (early rhizome fragmentation, ERF) and/or when conditions allowed after the first forage cut (late rhizome fragmentation, LRF). Cold, wet springs and hard, dry soil in summer delayed treatment in both treatment years, to late spring (ERF) and late summer/early autumn (LRF). In the treatment year, ERF reduced first-cut forage yield by 44% compared with no rhizome fragmentation, while LRF decreased second- and third-cut yield by 24% and 53%, respectively. In the year after treatment, ERF increased total forage yield by on average 10%, while LRF had no effect. Over both years, combined forage yield was reduced by 11% by ERF and 4% by LRF. Both treatments reduced E. repens rhizome biomass, but inconsistently (ERF by 25% in one year only, LRF by 24% at one of two sites). ERF reduced E. repens incidence in forage by 10% in the treatment year, but had no effect in the following year. Thus, rhizome fragmentation by vertical disking can reduce E. repens abundance in grass-clover leys, but the effect is inconsistent and forage yield can be impaired, especially in swards with much E. repens. Moreover, disking is hampered by hard, dry soil conditions.

Environmental consequences of pig production scenarios using biomass from rotational grass-clover leys as feed

Production of pork based on monoculture cereal-based cropping systems causes substantial environmental pressures and feed-food competition. This study evaluated the environmental consequences of five different scenarios involving inclusion of rotational grass-clover leys and incorporation of grass-clover biomass in pig diets: (1) a conventional reference scenario without grass-clover biomass; (2) a conventional scenario with replacement of feed with grass-clover silage in a total mixed ration, i.e., with grass-clover biomass replacing other feed; (3) an organic scenario using grass-clover silage for enrichment purposes only; (4) an organic scenario using grass-clover silage for enrichment purposes and additional grass-clover leys for green manuring; and (5) an organic scenario using grass-clover silage and pasture to replace feed. The functional unit was 1 kg of pork slaughter weight and the system boundary was from cradle to farm gate. We used life cycle assessment, the introductory carbon balance method and human edible feed conversion efficiency to assess the performance of the pig production system. Introducing grass-clover biomass as a total mixed ration in conventional pig diets, reduced the climate impact (-17%), eutrophication (-7.1%), marine eutrophication (-15%), energy use (-13%), and feed-food competition (-20%) per kg of pork meat, while acidification (+2.7%) and land use (+1.5%) were slightly increased compared with the reference. The lower climate impact (without considering soil carbon change) was attributable to reduced fertilizer and diesel needs due to pre-crop effects. Overall, feeding grass-clover biomass decreased several environmental impact categories, feed-food competition and improved cereal-based cropping systems by the introduction of grass-clover leys.

Diversified crop rotations and organic amendments as strategies for increasing soil carbon storage and stabilisation in UK arable systems

Adaptations in crop rotation with the inclusion of temporary grass-clover leys and organic amendments, have been promoted as effective ways to improve soil carbon (C) sequestration and mitigate climate change in agricultural systems. However, there are still a lot of uncertainties related to i) the combined effects of different crop rotations and different fertilisation sources, e.g., organic amendments, on soil C stocks; and ii) their potential effect on C stabilisation. The objective of this study was to evaluate the effect of different arable crop rotations with varying degrees of diversity in crop type and lengths of grass-clover ley periods and fertilisation sources on soil C stocks and C stabilisation down to 0.60 m soil depth. This was investigated in a long-term factorial field experiment-combining different crop rotation (cereal-intensive conventional vs. diversified legume-intensive organic) with different lengths of grass-clover ley periods (2 vs. 3 years), fertilisation sources (mineral vs. compost), and years (samples taken at the beginning and at the last year of one complete cycle of rotation; 8 years apart)-to explore their individual and interactive effect on soil C stock and C stabilisation at two soil depths (0–0.30 and 0.30–0.60 m). Soil C stabilisation was assessed using a unique combination of three different techniques: physical fractionation for separation of C associated to organic and mineral fractions, thermal analysis combined with differential scanning calorimetry and a quadrupole mass spectrometry (TG-DSC-QMS) for physical-chemical aspects, and pyrolysis coupled with gas chromatography-mass spectrometry (Py-GC/MS) for molecular structural information. The findings showed higher soil C stocks under the diversified organic rotation with 3 years of grass-clover ley period at both soil depths, regardless of the fertilisation source or sampling year. However, the organic rotation seemed to deliver stable soil C stocks only in the subsoil layer. Compost fertilisation, in turn, increased topsoil C stocks between the two sample dates under both rotations, and it appears to be stable. These results suggested that combining a diversified organic rotation with 3 years grass-clover ley with compost fertilisation could be one way for agricultural systems to deliver stable soil C sequestration.

Grass-clover response to cattle slurry N-rates: Yield, clover proportion, protein concentration and estimated N2-fixation

Optimal nitrogen (N) fertilization for grass-clover leys is important, since the N application affects yields and the clover and crude protein (CP) contents of the forage. Modeling of optimal N application to maximize economic output and minimize environmental consequences depends on grass-clover responses, e.g., on clover proportion, dry matter (DM) and CP yield. Thus, such parameters need to be quantified in field trials. Based on a three-year field trial (2017–2019; Foulumgaard Experimental Farm, Denmark) with four cuts of an organically managed grass-clover ley, the effect of increasing N fertilization from cattle slurry (0, 50, 100, 200 and 300 kg total N ha -1 ) on DM yield, CP yield and concentration as well as on clover proportion was examined. Results were analyzed separately for four sward ages (1–4 years). Dry matter yields ranged from 5.8 to 11.8 Mg ha -1 and the 200 and 300 kg N ha -1 treatments showed the highest DM yield for all sward ages; the 300 kg N ha -1 showed no significant additional yield benefit relative to the 200 kg N ha -1 . The clover proportion generally decreased with increasing N application (36–65 % for 0 N and 8–35 % for 300 kg N ha -1 ). The herbage concentration of CP was either unaffected or decreased with increasing N application. The fourth-year swards showed DM yields comparable to first and third-year sward ages. In unfertilized swards, a linear relationship was found between the first cut in a given year and the fourth cut of the previous year ( r 2 = 0.82 within a range of autumn clover proportions of 33–74 %), indicating the possibility of using last cut for predicting next year’s fertilizer requirements. The comprehensive data and results may form the basis for future modeling of optimal N application in organic grass-clover leys. • Dry matter yield increased up to a fertilizer level of 200 kg N for all sward ages. • Crop N concentration showed decreasing tendency with increasing slurry N application. • Knowledge on optimal N application to organic grass-clover ley provided.

Soil macroaggregation drives sequestration of organic carbon and nitrogen with three-year grass-clover leys in arable rotations

Conventional arable cropping with annual crops established by ploughing and harrowing degrades larger soil aggregates that contribute to storing soil organic carbon (SOC). The urgent need to increase SOC content of arable soils to improve their functioning and sequester atmospheric CO2 has motivated studies into the effects of reintroducing leys into long-term conventional arable fields. However, effects of short-term leys on total SOC accumulation have been equivocal. As soil aggregation may be important for carbon storage, we investigated the effects of arable-to-ley conversion on cambisol soil after three years of ley, on concentrations and stocks of SOC, nitrogen and their distributions in different sized water-stable aggregates. These values were benchmarked against soil from beneath hedgerow margins. SOC stocks (0–7 cm depth) rose from 20.3 to 22.6 Mg ha−1 in the arable-to-ley conversion, compared to 30 Mg ha−1 in hedgerows, but this 2.3 Mg ha−1 difference (or 0.77 Mg C ha−1 yr−1) was not significant). However, the proportion of large macroaggregates (> 2000 μm) increased 5.4-fold in the arable-to-ley conversion, recovering to similar abundance as hedgerow soils, driving near parallel increases in SOC and nitrogen within large macroaggregates (5.1 and 5.7-fold respectively). The total SOC (0–7 cm depth) stored in large macroaggregates increased from 2.0 to 9.6 Mg ha−1 in the arable-to-ley conversion, which no longer differed significantly from the 12.1 Mg ha−1 under hedgerows. The carbon therefore accumulated three times faster, at 2.53 Mg C ha−1 yr−1, in the large macroaggregates compared to the bulk soil. These findings highlight the value of monitoring large macroaggregate-bound SOC as a key early indicator of shifts in soil quality in response to change in field management, and the benefits of leys in soil aggregation, carbon accumulation, and soil functioning, providing justification for fiscal incentives that encourage wider use of leys in arable rotations.

Experimental evaluation of biological regeneration of arable soil: The effects of grass-clover leys and arbuscular mycorrhizal inoculants on wheat growth, yield, and shoot pathology.

Wheat yields have plateaued in the UK over the last 25 years, during which time most arable land has been annually cropped continuously with short rotations dominated by cereals. Arable intensification has depleted soil organic matter and biology, including mycorrhizas, which are affected by tillage, herbicides, and crop genotype. Here, we test whether winter wheat yields, mycorrhization, and shoot health can be improved simply by adopting less intensive tillage and adding commercial mycorrhizal inoculum to long-term arable fields, or if 3-year grass-clover leys followed direct drilling is more effective for biological regeneration of soil with reduced N fertiliser. We report a trial of mycorrhization, ear pathology, and yield performance of the parents and four double haploid lines from the Avalon x Cadenza winter wheat population in a long-term arable field that is divided into replicated treatment plots. These plots comprised wheat lines grown using ploughing or disc cultivation for 3 years, half of which received annual additions of commercial arbuscular mycorrhizal (AM) inoculum, compared to 3-year mown grass-clover ley plots treated with glyphosate and direct-drilled. All plots annually received 35 kg of N ha−1 fertiliser without fungicides. The wheat lines did not differ in mycorrhization, which averaged only 34% and 40% of root length colonised (RLC) in the ploughed and disc-cultivated plots, respectively, and decreased with inoculation. In the ley, RLC increased to 52%. Two wheat lines were very susceptible to a sooty ear mould, which was lowest in the ley, and highest with disc cultivation. AM inoculation reduced ear infections by >50% in the susceptible lines. In the ley, yields ranged from 7.2 to 8.3 t ha−1, achieving 92 to 106% of UK average wheat yield in 2018 (7.8 t ha−1) but using only 25% of average N fertiliser. Yields with ploughing and disc cultivation averaged only 3.9 and 3.4 t ha−1, respectively, with AM inoculum reducing yields from 4.3 to 3.5 t ha−1 in ploughed plots, with no effect of disc cultivation. The findings reveal multiple benefits of reintegrating legume-rich leys into arable rotations as part of a strategy to regenerate soil quality and wheat crop health, reduce dependence on nitrogen fertilisers, enhance mycorrhization, and achieve good yields.

Soil organic C and N stock changes in grass-clover leys: Effect of grassland proportion and organic fertilizer

Grass-clover leys in crop rotations on dairy farms may contribute to mitigation of climate change through soil organic carbon (SOC) sequestration. However, accurately quantifying SOC sequestration potential of grass-clover leys is a challenge as long-term experiments with regular soil sampling and crop rotations with varying grassland proportion are extremely rare.Based on a long-term organic dairy crop rotation experiment at Foulumgaard Experimental Station (Denmark) initiated in 1987 with contrasting crop rotations (2 or 4 years of grass-clover in a six-course rotation) and cattle slurry input from 2006 to 2018, we examined the effect of grassland proportion (31 to 69% grass-clover) and slurry C-input (0 to 1.45 Mg C ha−1 yr−1) on SOC storage in the top soil layer (0–20 cm).At steady-state conditions, the SOC stock based on equivalent soil mass (SOC stockFM) increased by 5.0 Mg C ha−1 when converting soil with a prehistory of cereal dominated cropping to a crop rotation with 1/3 grass-clover. The steady-state condition was established after 20 years, and the rate of change was largest in the initial years. A multiple regression model including grassland proportion, slurry C-input and the initial SOC stockFM explained 47% of the variation in the SOC stock from 2005 to 2018. The SOC stockFM increased 4.2 Mg C ha−1 when increasing the grassland proportion from 1/3 to 2/3 during the 13 years (0.32 Mg C ha−1 yr−1). Of the applied slurry-C, 11% was retained in soil, which means that an annual increase in cattle slurry input of 1.5 Mg C ha−1 (150 kg total-N ha−1) will increase SOC stockFM by 2.2 Mg C ha−1 (0.17 Mg C ha−1 yr−1) during the 13 years. Further, our study showed that soil nitrogen is stored along with SOC in equal proportions.

Integrating Crop-Livestock System Practices in Forage and Grain-Based Rotations in Northern Germany: Potentials for Soil Carbon Sequestration

Integrating leys, cover crops, and animal manures constitute promising avenues to reach annual soil organic carbon changes (ΔSOC) >0.4% in forage and grain-based crop rotations, rates required to offset the increasing C emissions from fossil fuels (“4 per mille” initiative). How these practices and rotations perform in reaching this aim was object of analysis in this paper. Five cropping systems (CS), including three three-year forage and grain-based crop rotations containing annual grass-clover leys (FR and MR) or cover crops (GR), and two contrasting controls (continuous silage maize (CM), and permanent grassland (PG)) were compared for their impact on SOC stocks over eight years (2010–2018). The CS were unfertilized (N0) or fertilized using cattle slurry (N1) at a rate of 240 kg N ha−1 yr−1 applied in the non-leguminous crops. The ΔSOC of the top 30 cm soil layer and the annual carbon inputs (Cin) from slurry applications and plant residues were estimated, their relationship established, and the slurry-induced C retention coefficient was determined. The FR and MR SOC stocks remained stable at N1, while the GR and CM SOC decreased over time by tendency even at N1. Only the PG reached ΔSOC >0.4%. Differences in ΔSOC between CS and N rates were highly associated with the system-specific increase in belowground Cin, induced by slurry applications. Slurry-induced C retention coefficients differed strongly between CS: CM (3%) followed by GR (12%), and by FR and MR (20–15%), and lastly by PG (24%). Promoting belowground carbon inputs was identified as an efficient way to reach significant increases in ΔSOC. We conclude that a ley in only one out of three years is not sufficient to significantly increase SOC stocks in arable crop rotations of the study region.

Field-scale monitoring of nitrate leaching in agriculture: assessment of three methods

Deterioration of groundwater quality due to nitrate loss from intensive agricultural systems can only be mitigated if methods for in-situ monitoring of nitrate leaching under active farmers’ fields are available. In this study, three methods were used in parallel to evaluate their spatial and temporal differences, namely ion-exchange resin-based Self-Integrating Accumulators (SIA), soil coring for extraction of mineral N (Nmin) from 0 to 90 cm in Mid-October (pre-winter) and Mid-February (post-winter), and Suction Cups (SCs) complemented by a HYDRUS 1D model. The monitoring, conducted from 2017 to 2020 in the Gäu Valley in the Swiss Central Plateau, covered four agricultural fields. The crop rotations included grass-clover leys, canola, silage maize and winter cereals. The monthly resolution of SC samples allowed identifying a seasonal pattern, with a nitrate concentration build-up during autumn and peaks in winter, caused by elevated water percolation to deeper soil layers in this period. Using simulated water percolation values, SC concentrations were converted into fluxes. SCs sampled 30% less N-losses on average compared to SIA, which collect also the wide macropore and preferential flows. The difference between Nmin content in autumn and spring was greater than nitrate leaching measured with either SIA or SCs. This observation indicates that autumn Nmin was depleted not only by leaching but also by plant and microbial N uptake and gaseous losses. The positive correlation between autumn Nmin content and leaching fluxes determined by either SCs or SIA suggests autumn Nmin as a useful relative but not absolute indicator for nitrate leaching. In conclusion, all three monitoring techniques are suited to indicate N leaching but represent different transport and cycling processes and vary in spatio-temporal resolution. The choice of monitoring method mainly depends (1) on the project’s goals and financial budget and (2) on the soil conditions. Long-term data, and especially the combination of methods, increase process understanding and generate knowledge beyond a pure methodological comparison.

Scenario analysis using the Daisy model to assess and mitigate nitrate leaching from complex agro-environmental settings in Denmark

Nitrate (N) leaching from intensively managed cropping systems is of environmental concern and it varies at local scale. To evaluate the performance of agricultural practices at this scale, there is a need to develop comprehensive assessments of N leaching and the N leaching reduction potential of mitigation measures. A model-based analysis was performed to (i) estimate N leaching from Danish cropping systems, representing 20 crop rotations, 3 soil types, 2 climates and 3–4 levels of manure (slurry)-to-fertilizer ratios, but with same available N (according to regulatory N fertilization norms), and (ii) appraise mitigation potential of on-farm measures (i.e. catch crops, early sowing of winter cereals) to reduce N leaching. The analysis was performed using a process-based agro-environmental model (Daisy). Simulated average N leaching over 24 years ranged from 16 to 85 kg N/ha/y for different crop rotations. Rotations with a higher proportion of spring crops were more prone to leaching than rotations having a higher proportion of winter cereals and semi-perennial grass-clover leys. N leaching decreased with increasing soil clay content under all conditions. The effect of two climates (different regions, mainly differing in precipitation) on N leaching was generally similar, with slight variation across rotations. Supplying a part of the available N as manure-N resulted in similar N leaching as mineral fertilizer N alone during the simulation period. Among the mitigation measures, both undersown and autumn sown catch crops were effective. Effectiveness of measures also depended on their place and frequency of occurrence in a rotation. Adopting catch crops during the most leaching-prone years and with higher frequency were effective choices. This analysis provided essential data-driven knowledge on N leaching risk, and potential of leaching reduction options. These results can serve as a supplementary guiding-tool for farmers to plan management practices, and for legislators to design farm-specific regulatory measures.

Very Low Nitrogen Leaching in Grazed Ley-Arable-Systems in Northwest Europe

High input dairy farms that are located on sandy soils in northwest Europe are predisposed to substantial nitrate leaching during a surplus of winter precipitation. Leys within integrated crop-livestock systems play an important role in soil fertility, soil C sequestration and soil N mineralization potentials. Therefore, leys are a feasible option that can be utilized to reduce local N losses to the environment, especially following maize grown for silage. We hypothesize that grass-clover leys ensure low nitrate leaching losses even when grazed intensively. The extent to which NO3-leaching occurred across seven different pasture management systems in terms of their sward composition, cutting, grazing, fertilization and combinations thereof was investigated in integrated animal-crop grazing systems over three winter periods (2017/2018, 2018/2019 and 2019/2020). The observed grazed systems were comprised of cut-used- and grazed grass-clover swards (0, 1 and 2 years after establishment following cereals), a catch crop grazed late in the year as well as a cut-used permanent grassland for comparison. Overall, all treatments resulted in nitrate leaching losses that did not exceed the WHO-threshold (25 mg nitrate/L). The highest level of NO3-leaching was observed in the catch crop system and the lowest in cut-used permanent grassland, with NO3-N losses of 19.6 ± 5.3 and 2.1 ± 0.3 kg NO3-N ha−1 year−1. Annual herbage yields were in the range of 0.9 to 12.4 t DM ha−1 and nitrogen yields varied between 181 ± 51 and 228 ± 66 kg N ha−1 during the study period. The highest herbage-N-yields were observed from the 1- and 2-year-old grass-clover leys. The highest N-field-balance was observed for the grazed leys and the lowest for the cut-used permanent grassland. However, no correlation was found between the highly positive field-N-balance and the amount of NO3-leached. This indicates a high N carry-over from grass-clover swards to the subsequent cash crop unit instead of increasing the risk of groundwater contamination from grazed leys in integrated animal crop-systems and underlines the eco-efficiency of dairy farming based on grazed ley systems.

Clover increases N2O emissions in boreal leys during winter

Inclusion of clover in grasslands increases functional diversity, N yield and forage quality and has been advocated for mitigating nitrous oxide (N2O) emissions. However, boreal grass-clover leys often show poor winter survival with considerable aboveground losses of nitrogen (N) and carbon (C). Little is known about how these losses affect off-season N2O emissions. Here we report field experiments over two winters, conducted at two coastal locations in Western and Northern Norway. N2O emissions were measured in plots with 0, 30 and 100% red (T. pretense) and white clover (T. repens) in a timothy - meadow fescue mixture. Overwinter N loss from the sward was quantified by comparing N contents in roots, stubble and herbage in autumn and spring. Additional treatments were removal of above-ground biomass in autumn and soil compaction. Off-season N2O emissions correlated positively with estimated overwinter N loss from herbage, which in turn depended on the fraction of clover in the ley. Pure grass leys emitted less N2O than leys that contained clover. Corrected for background emissions from pure grass, up to 13% of the above-ground N loss was emitted as N2O–N when clover was grown in pure stand. This fraction was much smaller, however, when clover was grown in mixture with grass (1.9 ± 0.9%), suggesting reassimilation of inorganic N. Indeed, we found significant increases in root and stubble N in mixtures throughout winter. Removal of above-ground biomass in autumn appeared to reduce the sward's ability to retain N throughout winter, and hence had no or a stimulating effect on N2O emissions. Soil compaction increased off-season N2O emissions 1.3–1.6-fold. Our results show that boreal grass-clover leys can be a significant source of N2O during winter, intricately controlled by loss and reassimilation of N. This underscores the importance of off-season plant-soil management for reducing the greenhouse gas (GHG) footprint of animal production in high latitude ecosystems.

Toward Specialized or Integrated Systems in Northwest Europe: On-Farm Eco-Efficiency of Dairy Farming in Germany

Intensive confinement (IC) systems for dairying have become widespread during the last decades. However, potential advantages of alternative systems such as full-grazing (FG) or integrated dairy/cash-crop (IFG) systems with regards to better provision of ecosystem services are widely discussed. To investigate performance and environmental impacts, we compared four prevailing dairy systems using an on-farm research study. The farm types differed in their share of pasture access and quantity of resource inputs: (i) an IC with a high import of supplements and mineral fertilizers; (ii) a semi-confinement (SC) with daytime pasture access during summer and moderate import of supplementary feeds representing the base-line scenario; (iii) a FG based on grazed seeded grass-clover swards with no purchased N-fertilizers and low quantities of supplementary feeds; and (iv) an IFG comparable to FG but based on grass-clover leys integrated in a cash-crop rotation. Results revealed highest milk productivity (16 t energy-corrected-milk (ECM) ha−1) and farm-N-balance (230 kg N ha−1) in IC; however, the highest product carbon footprint (PCF; 1.2 CO2eq kg ECM−1) and highest N-footprint (13 g N kg ECM−1) were found in the baseline system SC. The FG and IFG revealed on average similar forage dry matter yields (10 – 11 t DM ha−1) at similar crude protein and net-energy-lactation ratios per kg DM-intake compared to the IC and SC. The PCF in FG were comparable to IC (0.9 vs. 1.1 kg CO2eq kg ECM−1) but at a lower N-footprint (9 vs. 12 g N kg ECM−1). However, despite low measured N-losses in the FG system, the farm-N-surplus was exceeded by 90 kg N ha−1. A further reduction was only possible in the IFG (50 kg N ha−1) by accounting for a potential N-carry-over from N-rich plant residues to the cash-crop unit, leading to the lowest PCF (0.6 kg CO2eq kg ECM−1) for the IFG, with still moderate milk yield levels (~10,500 kg ECM ha−1). According to this bottom-up approach based on field data, improved integrated grazing systems could provide an important opportunity to increase the ecosystem services from dairy farming, operating with land use efficiencies similar to IC.

Arable fields as potential reservoirs of biodiversity: Earthworm populations increase in new leys

Managing soil to support biodiversity is important to sustain the ecosystem services provided by soils upon which society depends. There is increasing evidence that functional diversity of soil biota is important for ecosystem services, and has been degraded by intensive agriculture. Importantly, the spatial distribution of reservoirs of soil biota in and surrounding arable fields is poorly understood. In a field experiment, grass-clover ley strips were introduced into four arable fields which had been under continuous intensive/conventional arable rotation for more than 10 years. Earthworm communities in arable fields and newly established grass-clover leys, as well as field boundary land uses (hedgerows and grassy field margins), were monitored over 2 years after arable-to-ley conversions. Within 2 years, earthworm abundance in new leys was 732 ± 244 earthworms m−2, similar to that in field margin soils (619 ± 355 earthworms m−2 yr−1) and four times higher than in adjacent arable soil (185 ± 132 earthworms m−2). Relative to the arable soils, earthworm abundance under the new leys showed changes in community composition, structure and functional group, which were particularly associated with an increase in anecic earthworms; thus new leys became more similar to grassy field margins. Earthworm abundance was similar in new leys that were either connected to biodiversity reservoirs i.e. field margins and hedgerows, or not (installed earthworm barriers). This suggests that, for earthworm communities in typical arable fields, biodiversity reservoirs in adjacent field margins and hedgerows may not be critical for earthworm populations to increase. We conclude that the increase in earthworm abundance in the new leys observed over 2 years was driven by recruitment from the existing residual population in arable soils. Therefore, arable soils are also potential reservoirs of biodiversity.

Soil quality regeneration by grass-clover leys in arable rotations compared to permanent grassland: Effects on wheat yield and resilience to drought and flooding

Intensive arable cropping depletes soil organic carbon and earthworms, leading to loss of macropores, and impaired hydrological functioning, constraining crop yields and exacerbating impacts of droughts and floods that are increasing with climate change. Grass and legume mixes traditionally grown in arable rotations (leys), are widely considered to regenerate soil functions, but there is surprisingly limited evidence of their effects on soil properties, resilience to rainfall extremes, and crop yields. Using topsoil monoliths taken from four intensively cropped arable fields, 19 month-old grass-clover ley strips in these fields, and from 3 adjacent permanent grasslands, effects on soil properties, and wheat yield in response to four-weeks of flood, drought, or ambient rain, during the stem elongation period were evaluated. Compared to arable soil, leys increased earthworm numbers, infiltration rates, macropore flow and saturated hydraulic conductivity, and reduced compaction (bulk density) resulting in improved wheat yields by 42–95 % under flood and ambient conditions. The leys showed incomplete recovery compared to permanent grassland soil, with modest gains in soil organic carbon, total nitrogen, water-holding capacity, and grain yield under drought, that were not significantly different (P > 0.05) to the arable controls. Overall, grass-clover leys regenerate earthworm populations and reverse structural degradation of intensively cultivated arable soil, facilitating adoption of no-tillage cropping to break out of the cycle of tillage-driven soil degradation. The substantial improvements in hydrological functioning by leys will help to deliver reduced flood and water pollution risks, potentially justifying payments for these ecosystem services, especially as over longer periods, leys increase soil carbon sequestration.

Resource-efficient use of land and animals—Environmental impacts of food systems based on organic cropping and avoided food-feed competition

Current food systems are resource-inefficient, as farm animals consume large quantities of human-edible crops and large amounts of external fossil fuel-based inputs are used for energy and fertilisers. In this study, we assessed the production capacity and environmental performance of an alternative theoretical regional food system based on organic production, avoided food-feed competition and agriculture that is self-sufficient in bioenergy. Livestock in the system are reared solely on feeds that do not compete with food production, i.e. grass from permanent pastures, temporary grass-clover leys and food industry by-products. We modelled the effect of this food system on food production, land use, environmental impacts and nutrient flows, using the Nordic region as a case. As crop rotations under organic farming need leguminous forage crops to supply nitrogen and control weeds, substantial amounts of grass biomass suitable for feeding ruminants are produced in the system. Modelling showed that such a food system could feed 109% of the projected Nordic population in 2030 in a scenario where ruminant production is limited by the availability of semi-natural grasslands, and 130% in a scenario in which all grass biomass produced in organic crop rotations is used as animal feed. However, even when all grass biomass is used for animal feed, the associated reduction in meat production led to diets with 81% less meat compared to current consumption in the Nordic region. Using all ley from the organic crop rotations as livestock feed would result in greater total food output and reduced land use per person, but also a larger climate impact per person due to more livestock production. There is thus a trade-off between optimising the food system for efficient land use or for ‘climate efficiency’. Assessments of nutrient supply showed nitrogen and phosphorus deficits in both scenarios, but particularly in the scenario in which all grass biomass is used for animal feed, due to nutrient losses in animal production. Increased recycling from society and other innovative sources of essential soil nutrients are needed to counterbalance removal and losses. Through utilising leftover streams and hence minimising food-feed competition and reducing livestock production, we show that organic agriculture can maintain high food output, sufficient to feed the future Nordic population and more, despite lower yields.