Maconellicoccus Hirsutus Research Articles

Biological effectiveness of organic and inorganic products for the management of Maconellicoccus hirsutus green in soursop crop

The pink mealybug Maconellicoccus hirsutus Green is a pest of economic importance in various crops around the world. The objective was to determine the biological effectiveness of organic and inorganic products for the management of M. hirsutus in the soursop crop. The experiment was carried out in the communities of “Lima de Abajo” and “El Capomo”, in the municipality of Compostela, Nayarit, Mexico, during the period from September to November 2018 and from July to August 2019. The treatments were Ara3, Inter-a-ch-c, Evolution, a complex of plant extracts (Capsicum chinense + Allium sativum + Allium cepa + Artemisia absinthium), Mos Blanc® (Imidacloprid), farmers’ control (Detergent Blanca Nieves® + Sodium Hypochlorite Cloralex®) and the absolute control (Water). The nymphs and adult females of M. hirsutus were counted after each application, in young shoots. The results showed that in “Lima de Abajo”, Ara 3 registered the highest percentage of suppression after 28 days. In “El Capomo”, Imidacloprid recorded the greatest suppression after 28 days of application. In both communities, the treatments Ara 3, Evolution and Imidacloprid registered the least number of living individuals. The results indicated that Ara 3 and Evolution were the most promising alternatives in the management against M. hirsutus in the crop.

Predatory potential of Cryptolaemus montrouzieri Mulsant on mealybugs and aphids

The predatory potential of Cryptolaemus montrouzieri Mulsant on mealybugs and aphids viz , Maconellicoccus hirsutus , Phenacoccus solenopsis and Aphis gossypii was studied at the Biocontrol Agents Laboratory, Department of Agricultural Entomology, College of Agriculture, Latur at the temperature of 25±2 ° C maintained in BOD incubator during year 2013-14. The consumption rate of grubs and beetles of C. montrouzieri was in order of A. gossypii > M. hirsutus > P. solenopsis .

CAN THE INTERACTION BETWEEN CASTOR AND JATROPHA OILS BE EFFICIENT IN THE MANAGEMENT OF PINK MEALYBUG?

The pink mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) is a polyphagous pest species that has been reported in about 350 species of host plants worldwide. Usually, the use of synthetic chemicals is still the most used control method for the control of agricultural pests. The objective of this present work was to evaluate the potential of the interaction of oils extracted from the species Ricinus communis L. (castor) and Jatropha curcas L. (jatropha) (Euphorbiaceae) in the form of direct and indirect application on M. hirsutus. The experiments were conducted in air-conditioned chambers at a temperature of 25 ± 1ºC, relative humidity 70% ± 10 and a photophase of 12h. The 3% concentration was used in the tests, with 11 interaction ratios between the oils. Individuals' mortality was assessed at 24, 48 and 72 hours after spraying. The results of the interactions indicated up to 78% mortality of individuals in the ratio 90% castor + 10% jatropha via indirect application, while in the direct application on individuals they presented 53% mortality in the ratio 40% castor + 60% jatropha. The interactions between castor and jatropha oils have demonstrated potential in the management of pink mealybug by direct or indirect application.

Exploring Momardica Charantia Leaves as Biopesticide Against Mealy Bugs: A Comparative Study

A pesticide is a substance used for controlling pests that damage the crops. Azadirachta indica (Neem) is widely studied and commercially used as a biopesticide against various pests. The present study aims to explore Momardica charantia (Bitter gourd) leaves as a biopesticide and compare its effectiveness with the Azadirachta indica (Neem) leaves and chemical pesticide (Admire). The test plant used for study is Hibiscus rosa-sinensis infected with Red Hibiscus mealy bug. Soil parameters (pH, conductivity, organic matter content, available potassium and available phosphorus) and plant characteristics (height, leaves count, flowering and chlorophyll content) of all test plants under study were estimated and compared. The soil parameter like pH showed no changes in control and treated plants while organic matter, available potassium and available phosphorus comparatively showed slight increase in concentration in plants treated with all pesticides. The plant characteristics viz. height, leaf count and branching showed improvement in growth after treatment with pesticides.All the pesticides under study were capable to kill mealy bugs in one month. Even though the biopesticidal property of Momardicacharantia leaves was found to be less as compared to Azadirachta indica leaves and chemical pesticide (Admire), it can be used effectively to control mealy bugs infecting Hibiscus plant.

Spatial Distribution Patterns of Parthenolecanium corni (Hemiptera, Coccidae) and of the Ampelovirus GLRaV-1 and the Vitivirus GVA in a Commercial Vineyard.

Distribution patterns of the European fruit lecanium Parthenolecanium corni (Bouché) and of grapevine leafroll-associated virus-1 (GLRaV-1) and grapevine virus A (GVA) were monitored from 2003 to 2015 in a Riesling vine plot in the northeast of France. Virus spread was compared between two periods: 2003–2008 and 2009–2014. The percentage of infected vines increased from 54 to 78% for GLRaV-1 and from 14 to 26% for GVA. The spatial distribution of viruses and of P. corni was analysed using permutation tests and revealed an aggregative pattern. Virus distribution was not associated with the density of P. corni population on grapevines. However, GLRaV-1 and GVA spread mainly from initially infected vines. New GLRaV-1 and GVA infections were more frequent on vines near primarily infected vines, first anisotropically along the row, then between neighbouring rows. Virus spread was similar to those described in literature with grapevine mealybug species. This slow vine-to-vine progression suggests that P. corni was responsible for the virus spread, in accordance with the low mobility and low transmission capacities of its local population.

Modelling the current and future potential distribution of Maconellicoccus hirsutus (Green, 1908) a pest of importance for Mexico

Objective: to model the current and future potential distribution of Maconellicoccus hirsutus in order to identify changes in its distribution in Mexico.Design/methodology/approach: to select a final model, 2,154 occurrence points were used and MaxEnt with the help of the Kuenm package implemented in Rstudio. It was projected throughout Mexico, using six possible climate change scenarios with a proposed threshold (0.02).Results: a potential present surface of 1,159,335.5 km2 was found, corresponding to 59 % of the territory, indicating a greater suitability in coastal areas including the Gulf of Mexico, the entire Pacific slope, and the Yucatan peninsula. All scenarios show an increase in the potential area of up to 1,423,890.18 km2 in 2050 and 1,537,591.19 km2 in 2070.Study limitations/implications: predictions for 2050 and 2070 will depend on the climatic conditions that take place at the time, so specific studies in each region should be carried out, considering that the species may have adaptability to new climates which would imply constant monitoring. If an increase is observed in the distribution areas, the speciescould affect not only agricultural areas but also forest ecosystems.Findings/conclusions: the projections made for scenarios 2050 and 2070 show an increase in the area of infestation andenvironmental suitability for pink cochineal, mainly in the states of the Pacific watershed, the Yucatan peninsula, and theGulf of Mexico, with these areas being the most vulnerable.

Scanning Electron Microscopy of Ferrisia Malvastra (Mcdaniel) (Hemiptera: Pseudococcidae)

In the present study Ferrisia malvastra (McDaniel) is recorded for the first time in Sudan, since only four species were recorded there, i.e. Planococcus citri (Risso), Ferrisia virgata (Cockerell), Maconellicoccus hirsutus (Green) and Phenacoccus solenopsis Tinsley. The present study deals with the morphology of the female of this mealybug by using electron microscope. The ultrastructure of antennae, claw and its digitules, anal opening, valva, cerarius, circulus and wax producing pores were examined and illustrated. The result revealed that antennae consists of 8 segments, they were measured and their sensella were described. The claw denticle is absent and its digitules are knobbed. The circulus is present. There is only one pair of stout conical setae cerarii on the anal lobe, all of these features are used to distinguish between genera of Pseudococcidae. Also, the wax producing pores were illustrated; they are two types, the trilocular pores which produce the fine coiled wax filaments covering the body and forming the lateral wax filaments and the multilocular pores which surrounding the valva and produce the ribbon like wax which form ovisac.

Infection parameters of Heterorhabditis amazonensis (Nematoda: Heterorhabditidae) in different stages of Hibiscus pink mealybug.

The pink hibiscus mealybug Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) is an invasive pest of an enormous variety of crops and has become a concern in many parts of the world. Early attempts to control M. hirsutus with chemical insecticides and cultural methods have failed due to the cryptic habit of the insect. We assessed the entomopathogenic nematode Heterorhabditis amazonensis as a biological agent against different insect stages. Comparing different concentrations of the nematode, insect females were very susceptible, with more than 90% of the insects killed. In second and third nymphal stages mortality rates varied from 20 to 60% depending on the nematode concentration. The first nymphal stage as much less susceptible to nematodes due to their small size. The number of nematodes capable of invading the insect host did not vary between the different concentrations. However, the LC50 for females (35.2 IJ/insect), second and third nymphal stages (83.9 IJ/insect) demonstrated that H. amazonensis should be considered as a potential biocontrol agent of the pink hibiscus mealybug.

Inventory and abundance of mealybug species in immature and mature cocoa farms in Côte d'Ivoire

AbstractFrom 2013 to 2018, surveys were conducted in counties not previously surveyed in order to determine species of mealybugs present in the cocoa orchard in Côte d'Ivoire as well as their abundance according to the age of cocoa trees. Immature and mature cocoa trees were inspected to hand‐height in 5 and 29 counties infected with Cacao swollen shoot virus (CSSV). In each cocoa farm, mealybugs were searched for on fruits, leaves, flowers, twigs and trunks. Mealybug species were identified, and colonies were counted. Five mealybug species were identified on immature cocoa trees: Ferrisia virgata, Formicococcus njalensis, Planococcus citri, Planococcus kenyae and Pseudococcus longispinus. In addition to these species, four species, Dysmicoccus brevipes, Maconellicoccus hirsutus, Phenacoccus hargreavesi and Pseudococcus jackbeardsleyi were identified on mature cocoa trees. On immature cocoa trees, Fo. Njalensis, Pl. citri and Ps. longispinus comprised were, respectively, 35%, 33% and 19% of colonies, respectively. On mature cocoa trees, Fo. Njalensis and Pl. citri comprised 63.2% and 21.0%, and others species 15.8%. Nevertheless, the abundance of mealybug species varied according to the age of cocoa trees. The preferred organs of mealybugs were pods (74.1%) followed by twigs (13.4%) and flowers (7.4%). Previously, the mealybug Paracoccus burnerae (Brain) was found on Theobroma cacao, which is the first record for this species in Côte d'Ivoire and on this host‐plant.

On the origin of Anagyrus callidus (Hymenoptera: Encyrtidae), a parasitoid ofpink hibiscus mealybug Maconellicoccus hirsutus (Hemiptera: Pseudococcidae).

Anagyrus callidus Triapitsyn, Andreason Perring (Hymenoptera: Encyrtidae), recently described from southern California, USA and Mexico, is a primary parasitoid of the pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). This wasp species previously was misidentified as Anagyrus kamali Moursi, which was introduced and released as such in the United States (California and Florida) and Mexico. In this paper, the origin of A. callidus is shown to be from Taiwan, based on differences in the morphology and molecular sequences of voucher specimens of an earlier colony of the misidentified A. kamali from Puerto Rico (released in August 2002 in southern Florida), and specimens that originated in southern Taiwan.

Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana(Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green)in grapes

Grape ( Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity for India. Pink mealybug, Maconellicoccus hirsutus (Green) is one of the most important pests infesting grapes. Two entomopathogenic fungi were isolated from the field infected insects and were identified as Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.). The pathogenicity study showed that both the fungi were capable of infecting M. hirsutus . LC 50 values 1.4 × 10 6 and 1.0 × 10 7 conidia per ml was recorded for M. brunneum and B. bassiana , respectively. Evaluation of compatibility of these fungi with insecticides is important to develop bio-intensive management strategy for mealybugs. The compatibility of seven insecticides (emamectin benzoate, tolfenpyrad, imidacloprid, clothianidin, buprofezin, fipronil, spirotetramat) with these entomopathogens was evaluated under laboratory conditions. Compatibility studies based on sporulation, germination and vegetative growth of fungi showed that imidacloprid and emamectin benzoate were most compatible and tolfenpyrad and spirotetramat were highly incompatible with both the entomopathogens.

Bacterial volatiles from mealybug honeydew exhibit kairomonal activity toward solitary endoparasitoid Anagyrus dactylopii

Anagyrus dactylopii (Howard) (Hymenoptera: Encyrtidae) is a solitary koinobiont endoparasitoid of grapevine mealybugs and has been reported to parasitize 70% of mealybug populations naturally. In the present study, we have isolated and identified by employing a 16S rRNA technique, a total of ten cultivable bacteria from the honeydew of two species of grapevine mealybugs, Maconellicoccus hirsutus and Nipaecoccus viridis. In the honeydew of M. hirsutus, seven bacteria were found, which included Micrococcus luteus, Kocuria rosea, Bacillus aquimaris, Exiguobacterium aquaticum, Staphylococcus pasteuri, Bacillus oceanisediminis and Bacillus flexus. From the honeydew of N. viridis, three bacteria, viz. Bacillus firmus, Microbacterium testaceum and Pesudomonas oryzihabitans, could be recovered. Further, we have collected the headspace from 72 h active cultures of these honeydew-associated bacteria by sorption over adsorbent trap made up of 0.2 g porapak using dynamic headspace sampling technique with pull and push system and tested the behavioral responses of mated females of A. dactylopii to these volatiles in a Y-tube olfactometer. The mated females positively responded to volatiles from five bacteria, which included only two bacteria associated with the honeydew of M. hirsutus, i.e., K. rosea, and S. pasteuri and all the three bacteria from honeydew of N. viridis. This indicates an ability of A. dactylopii to exploit cues associated with the mealybug honeydew for host location. The GC–MS analysis of bacterial headspace revealed six volatile organic compounds (VOCs), viz. propanoic acid, 2-methyl-2,2-dimethyl-1-(2-hydroxy-1-methylethyl) propyl ester; tetradecane; dodecane; 10-heptadecen-8-ynoic acid, methyl ester; 10,13-octadecadiynoic acid, methyl ester and limonen-6-ol, pivalate. Interestingly, limonen-6-ol, pivalate has been found as a common constituent of volatiles from all the three bacteria that were isolated from honeydew of N. viridis. The possible exploitation of bacterial VOCs in enhancing populations of A. dactylopii and promoting conservation biological control of mealybugs in grape vineyards is discussed.

Toxicity Aqueous Extract of Castor Bean (Ricinus communis) to Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae)

The objective of this study was to evaluate the insecticidal effect of aqueous extract of castor bean (Ricinus communis) cake for the control of Maconellicoccus hirsutus. The toxicity tests were performed in the laboratory. Subsequently lethal concentrations 50 and 90% (LC50and LC90, respectively) were estimated. Tween80® + distilled water was used as surfactants and solvent respectively. The assay was performed in petri dishes containing a Coffea canephora leaf disc. The spraying was carried out in Torre de Potter spray tower. The extract was toxic to mealybug and the mortality data was adapted to the probit model. Estimates of LC50 and LC90 were 5.32% and 29.30%, respectively. It was concluded that the aqueous extract of R. communis cake is promising for the management of M. hirsutus.

Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) in Brazil: Recent Spread, Natural Enemies, and New Hosts

Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) is an invasive and highly polyphagous pest with a worldwide distribution in tropical and subtropical regions. This study reports the geographical distribution, natural enemies, and host plant species of M. hirsutus in Brazil 8 years after its first report in this country. Maconellicoccus hirsutus is now distributed in 11 Brazilian states, in all major geographic regions of the country. Samples (n = 103) of plants infested by M. hirsutus were collected in the state of Espirito Santo, Brazil, with 96% of them found within the isotherm 23 to 27 °C. Hevea brasiliensis (Euphorbiaceae), Theobroma bicolor (Malvaceae), and T. speciosum (Malvaceae) are reported for the first time as hosts of M. hirsutus, and Coccoloba uvifera (Polygonaceae), Morus alba (Moraceae), Punica granatum (Lythraceae), and Talipariti tiliaceum (Malvaceae) are new hosts of M. hirsutus in Brazil. Thirty-seven host plant species of M. hirsutus are now confirmed in Brazil.

Insects Associated with the Soursop (Annona muricata L.) Crop in Nayarit, Mexico

The objective of the study was to identify the entomofauna associated with soursop cultivated commercially in the municipalities of Compostela and San Blas in the state of Nayarit, Mexico. The entomofauna obtained from samples taken from Nov 2016 to Dec 2017 were morphologically identified using taxonomic keys. A total of 3,674 insects were collected, with 20 species being phytophagous, including the pink hibiscus mealybug Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) and an armored scale Pinnaspis sp. (Hemiptera: Diaspididae). There were 12 species of predators in the families Coccinellidae (Azya orbigera [Mulsant], Chilocorus sp., Cryptolaemus montrouzieri [Mulsant], Cycloneda sanguinea L., Cycloneda sp., Hippodamia convergens Guerin-Meneville, Olla v-nigrum [Mulsant], Paraneda pallidula guticollis [Mulsant], and Stethorus pinachi [Gordon & Chapin]), and Chrysopidae (Ceraeochrysa valida [Banks], Ceraeochrysa sp., and Chrysoperla externa [Hagen]), and 5 species of parasitoids of the genera Aphytis, Coccophagus, Encarsia (all Hymenoptera: Aphelinidae), Anagyrus, and Gyranusoidea (both Hymenoptera: Encyrtidae). The ladybird beetle P. pallidula guticollis was recorded for the first time in the state of Nayarit. Also, 9 phytophagous species are new records for soursop in Mexico.

Influence of Biotic and Abiotic Factors on Mulberry Mealybug, Maconellicoccus hirsutus (Green) during the Summer Season

Objectives: The present study was carried out to investigate the biology of Maconellicoccus hirsutus on Alba variety of mulberry under laboratory conditions and comparative infestation of M. hirsutus on Alba and purple varieties of mulberry under field conditions. Methods/Statistical Analysis: Mean and standard error was used to describe the results. Findings: The results indicated that the fecundity of M. hirsutus varied from 221 to 232 varied eggs per female. The pest completed its life cycle in 29.08 to 32.51 days. The infestation of M. hirsutus on different mulberry variety showed that purple variety was resistant than Alba variety with pest population of 8.43 ± 0.33 mealybugs per 10 cm twig compared with 9.73 ± 0.93 mealybugs on Alba variety. Application/Improvements: M. hirsutus is a serious polyphagous pest insect of mulberry and other plants. Therefore, it is suggested that detailed studies on population dynamics, natural enemies and population management of this mealybug should be carried out under agroecological conditions of Sindh, Pakistan. Keywords: Biotic and Abiotic, Maconellicoccus hirsutus, Mulberry Mealybug

Pest management scenario in underutilized fruit crops

Worldwide, many agricultural and horticultural crops are available as underutilized; of which, the fruit crops such as, jack, fig, annona, ber, tamarind, West Indian cherry, bael, wood apple, jamun, bread fruit, avocado, star gooseberry, karonda and mulberry are being consumed by the local habitants and have potential for value addition in the near future. These underutilized fruit species afford promising export-oriented opportunities for income generation and an increasing endorsement at national and international level is being given to tap the potential of such valuable plant species with high genetic diversity. Such attention stems out from developments over the last decade that have contributed to change the perception of people regarding the importance of such species and raised the issue on how best to pursue the promotion of underutilized species. However, production of these fruit crops is also being hampered by many pest and diseases which are being less focused. Among the insect pests, the shoot and fruit borer, Margaronia caesalis, is a major pest of jackfruit which alone can cause up to 30% yield loss (Agropedia, 2014). Likewise, the other less utilized fruit crops also suffer from pests like pink mealybug, Maconellicoccus hirsutus in annona, fruit fly, Carpomyia vasuviana, fruit borer, Meridarches scyrodes and leaf roller, Psorostichia zizyphi in ber, shoot and capsule borer, Conogethes punctiferalis in wood apple, fruit fly, Daccus correctus in jamun, scales, Aspidiotus tamarindi in tamarind, leaf webber, Diaphinia pulverulentalis and pink mealybug, M. hirsutus in mulberry and tea mosquito bug, Helopeltis antonii in avocado. Hence, before strategies can be developed to promote the use of these underutilized fruit crops, careful analysis is necessary to monitor and document the precise impacts of pests and their management practices. Also, if it were possible to predict likely flare up of these pests before they evolve in the field, it might be feasible to have strategies to circumvent the problems. With this view, an insight into the pest problems of underutilized fruit crops and the possible ways to overcome their impact is discussed.

Biodiversity of mealybugs, their host range and bio-control agents associated in different districts of Chhattisgarh plains

The survey of host range of mealybugs in five districts of Chhattisgarh plain regions namely Rajnandgaon, Balod, Dhamtari, Mahasamund and Raipur districts in which 132 plant species were observed. Among these, 72 plant species belonging to 28 families were identified as hosts out of which two species were of pulses (2.78%), one oilseed (1.39%), ten vegetables (13.89%), twelve fruits (16.67%), one fibre (1.39%), one sugarcane crop (1.39%), nineteen ornamentals (26.39%), fifteen medicinal (20.83%), two forest trees (2.78%) and nine plants under weeds (12.50%). The highest number of host plants of mealybugs were recorded in the family Malvaceae and Asteraceae followed by Fabaceae, Apocynaceae, Solanaceae, Rosaceae, Amaranthaceae and Lamiaceae. Among 72 plant species, 36 species of host plants were found having low infestation, 12 species had medium infestation and 24 plant species were observed to be highly infested by mealybugs. A total of eight mealybug species namely Ferrisia virgata Cockerell, Maconellicoccus hirsutus Green, Nipaecoccus viridis Newstead, Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis Tinsley, Rastrococcus iceryoides Green and seven species of their natural enemies i.e., Cheilomenes sexmaculata, Brumoides suturalis, Scymnus sp., Chrysoperla sp., spiders as a general predator and Aenasius sp. and Pseudleptomastix mexicana as parasitoid were observed during the present studies. Among the Mealybugs species, P. solenopsis Tinsley was noticed as the most dominant species which observed on 14 species of different host plants infested with maximum 46.66 percent.

Mealybug species (Hemiptera: Coccomorpha: Pseudococcidae) on soursop and sugar apple (Annonaceae) in North-East Brazil, with description of a new species of Pseudococcus Westwood.

Several species of Annonaceae are economically important fruit-tree crops in North-East Brazil, including Pernambuco state. However, in several regions within the state, the fruits are commonly infested by mealybugs (Hemiptera: Coccomorpha: Pseudococcidae). There is a lack of information about the mealybug species damaging this produce, so a survey of mealybug species associated with commercial sugar apple (Annona squamosa L.) and soursop (A. muricata L.) was conducted in the main production areas. The species Ferrisia virgata (Cockerell), Maconellicoccus hirsutus (Green), Planococcus minor (Maskell), Phenacoccus solenopsis Tinsley and Pseudococcus jackbeardsleyi Gimpel Miller were found on both Annona species. Dysmicoccus brevipes (Cockerell), Ferrisia dayslirii Kaydan Gullan and Ferrisia malvastra (MacDaniel) were found only on soursop; and Ferrisia cristinae Kaydan Gullan, Planococcus citri (Risso), Pseudococcus annonae sp. n. Pacheco da Silva Kaydan and Pseudococcus sp. were found only on sugar apple. The species F. cristinae, F. dasylirii, F. malvastra and Ph. solenopsis are recorded infesting these hosts for the first time. The most abundant mealybug species found were F. virgata, Pl. minor, Ps. jackbeardsleyi and M. hirsutus, often forming heavy infestations and damaging the fruits. A new species, Pseudococcus annonae sp. n. Pacheco da Silva Kaydan, is described and illustrated, and an identification key to the mealybug genera occurring on annonaceous species in the Neotropical region is also provided.

New Records of Parasitoids Hymenopteran (Hymenoptera: Chalcidoidea) Associated With Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) in the State of São Paulo

Survey of hymenoptera parasitoids of pink-hibiscus-mealybug, Maconellicoccus hirsutus (Green, 1908) infesting Hibiscus rosa-sinensis was conducted in two municipalities of state of São Paulo, Brazil from August 2016 to August 2017. Nine species of chalcidoid wasps were obtained: Anagyrus kamali Moursi (1948); Anagyrus sp. aff. mirtesae Noyes and Menezes (2000); Anagyrus sp.; Bothriocraera bicolor Compere and Zinna (1955); Cheiloneurus sp., Gyranusoidea deionae Noyes (2000); Prochiloneurus sp. (Encyrtidae); Aprostocetus sp. (Eulophidae); and Chartocerus sp. (Signiphoridae). Anagyrus kamali and G. deionae were the most frequent species, corresponding to 67.26% and 22.62% of the parasitoids, respectively. Bothriocraera bicolor, Chartocerus and G. deionae are recorded for the first time associated with M. hirsutus. Gyranusoidea deionae and B. bicolor are recorded for the first time in Brazil. The seasonal study of M. hirsutus and its parasitoids was carried out in January to December 2017on 16 hibiscus plants, aligned in the hedge in the experimental area of the FCAV/UNESP in Jaboticabal, SP. For this purpose, ovisacs, nymphs and adults of mealybug were counting on infested plants and the adult parasitoids collected in Möerick traps. Maconellicoccus hirsutus was observed during the periods of January-March and October-December, with the highest infestation in January and February. In the traps, a total of 92 specimens of pink-mealybug parasitoids were obtained: 65% belonging to A. kamali and 35% to G. deionae.