Powdery mildew disease (Erysiphe necator Schwein) is a significant threat to grape cultivation in vineyards. Severe yield and quality losses could occur in vineyards when this pathogen is not managed correctly. Several commercial grape varieties are highly susceptible to powdery mildew; therefore, large quantities of fungicide are applied throughout the growing season. Besides yields and quality, new grapevine varieties that are genetically resistant to powdery mildew are needed for sustainable viticulture. This study was conducted through molecular screening of powdery mildew resistance genes in nine different Vitis labrusca L. genotypes grown in Black Sea Region of Türkiye. After PCR amplifications using Ren1, Ren3 and Ren9 locus-specific primer, all genotypes Ren1; Ren 9 gene was detected in 3 genotypes. However, Ren3 gene was not detected in any genotypes. It was concluded based on present findings that Vitis labrusca L. genotypes with resistance genes could be used as genetic resources in grapevine breeding programs and significant economic benefits can be provided accordingly.