The grapes are being exported in increasing quantities from Maharashtra to European countries and a lot of pesticide inputs are being used by the growers. A total number of 578 grape samples collected from Nasik district during December, 2013 to April, 2014 and analyzed for 167 numbers of multi-class pesticide (Organophosphate, Triazine, Pyrimidine, Triazole, Imidazole, Benzimidazole, Nicotinoid, Substituted thiourea, Strobiluron, Dinitroaniline, Morpholine) residue levels using Liquid Chromatography-Mass spectrometry/Mass spectrometry (LC-MS/MS) and Gas Chromatography-Mass spectrometry/Mass spectrometry (GC-MS/MS) by using validated methods. Only four samples were free from pesticide residues and rest were contaminated with 1-13 numbers of pesticides residue. During the study different classes of total 41 number of agro-chemicals had been detected and 116 number of samples were failed with residues of 4-Bromo-2-Chlorophenol, Abamectin,Carbendazim, Chlormequat Chloride , Chloropyriphos,Dinocap,Forchlorfenuron, Hexaconazole, Flusilazole, Profenophos, Spinosad, Thiacloprid, Triazophos, Fipronil and Acephate by exceeding their European Union MRLs.