The decrease in cereal grain zinc (Zn) caused by phosphorus (P) application has attracted wide attention. However, optimizing P fertilization for both satisfactory grain yield and grain Zn concentration is still a problem due to a poor understanding of the relationship between P application rates and available soil P, and that of available soil P and soil Zn availability, relevant soil factors, and plant Zn uptake and utilization. A location-fixed field experiment was initiated in 2004 with winter wheat (Triticum aestivum L.) grown at five P rates of 0, 50, 100, 150, and 200 kg P2O5 ha−1, and soil and plant samples were collected during the three growing seasons of 2013–2016. Winter wheat grain yield increased, and the grain Zn concentration decreased with increasing available soil P in a linear-plus-plateau manner. The grain yield plateau, averaging 6009 ± 155 kg ha−1, was reached at an available soil P concentration of 10.2 ± 2.5 mg kg−1, and the grain Zn plateau, averaging 22.4 ± 0.9 mg kg−1, was reached at an available soil P of 14.2 ± 1.8 mg kg−1. Shoot Zn uptake after flowering was not affected, while Zn remobilization from vegetative parts to grains and the Zn harvest index increased with P application at available soil P levels below 11.6 mg kg−1. The available soil Zn increased, and root mycorrhizal colonization was unaffected at lower available soil P levels. The decrease in wheat grain Zn concentration with increasing P application at lower available soil P levels was primarily explained by yield dilution effects, not the changes in available soil Zn and root mycorrhizal colonization. Under the experimental conditions, the available soil P would have to be as low as 0.7 ± 0.4 mg kg−1 to achieve the target grain Zn concentration of 40 mg kg−1, and at this level, the grain yield would only be 4127 ± 252 kg ha−1.
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