In order to obtain the mixture or separation of the biological or chemical fluids with viscoelastic characteristics, the rotating electro-osmotic flow of fractional third grade fluids in a microchannel is investigated. The momentum equation is solved by Laplace and Fourier Transforms after linearization by small parameter perturbation method. Furthermore, the numerical solutions also are obtained by finite difference method. The velocity distribution influenced by different physical parameters is discussed. Two interesting phenomena can be observed. One of them is that reverse flow appears at the center of the channel for some physical parameters. Another one is that the velocity in the microchannel will arrive at a steady state from oscillation at last. However, with the increase of fractional derivative parameters, the amplitude of the oscillation becomes bigger at the center and the time arriving at the steady state is delayed.
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