Good Stability Research Articles

Fluidized spanlastics for intranasal brain delivery of lacosamide aiming to control Status Epilepticus: Design, Characterization, Ex-vivo permeation, Radioiodination and In-vivo biodistribution studies

Status Epilepticus (SE) is a neurological condition characterized by anomalous brain activity, causing repetitive seizures lasting for more than 5 min or periods of unusual behavior without returning to a normal level of consciousness between episodes. Chitosan has shown various pharmaceutical benefits, particularly in intranasal administration. In the current manuscript, novel ultra-deformable vesicles (fluidized spanlastics) loaded with lacosamide (LCM), a model anti-epileptic drug, were formulated and compared with their chitosan containing counterparts. The formed vesicles were assessed for their particle size (PS), zeta potential (ZP), entrapment efficiency (EE%) and storage stability. Surface morphology, pH and viscosity measurements, in-vitro release study and release kinetics, and ex-vivo permeation across nasal sheep mucosa were carried out on the optimized formulations. The bio-distribution and pharmacokinetic studies were performed using the radioiodinated-LCM (131I-LCM) utilizing different routes of administration to assess the in-vivo performance of intravenous solutions (IVs), intranasal solutions (INs) and the intranasal administered optimized formula (INFS). LCM-vesicles showed PS ranging from 109.90 to 418.4 nm, negative ZP values ranging from −28.00 to −48.30 mV in case of regular vesicles and shifted to +16.70 to +18.72 mV in case of chitosan coated vesicles, high EE% values having a range of 65.76–97.25 % and good storage stability properties. The optimized formulation showed spherical shape and enhanced ex-vivo permeation approximately 4-fold higher compared to LCM solution. The results of the in-vivo study demonstrated that the brain/blood ratio (B/B) of INs was 3.3-fold higher than IVs and INs of 131I-LCM in the first 5 min. Accordingly, the prepared system has been shown to possess a promising potential for brain diagnosis and/or therapy.

Enhancing the mechanical properties of polylactic acid (PLA) composite films using Pueraria lobata root microcrystalline cellulose

To enhance the mechanical properties of polylactic acid (PLA) material, the PLA-based composite films are prepared by using Pueraria lobata (Willd.) Ohwi root microcrystalline cellulose (PRMCC) treated with 3-aminopropyl triethoxysilane (KH550) silane coupling agent as the dispersed phase through solvent casting method. The effects of the concentrations of PRMCC and KH550 as well as the KH550 pretreating condition (ethanol concentration) on the tensile properties of PLA-based composite films are investigated. The PLA-based composite film treated with 5 wt% PRMCC and 18 wt% KH550 (pretreated by 90 % EtOH) exhibits the greatest performance. Its elongation at break value is detected to be 4.0 %, 1.6 times as large as that of pure PLA film. The water absorption of the as-prepared PLA-based composite film is reduced from 0.49 % of the unmodified PLA/PRMCC film to 0.12 %. Moreover, the modified PLA-based composite film has a hydrophobic surface and exhibits good thermal stability. Compared with pure PLA film, the modified PLA-based composite film exhibits improved UV shielding performance with acceptable transparency. Furthermore, after adding poly(butylene adipate-co-terephthalate) (PBAT) to the composite system, the elongation at break of the PLA-based composite film is up to 7.2 %. This research can provide theoretical guidance for enhancing the performance of PLA products.

Anionic Solvation Transition at Low Temperatures for Reversible Anodes in Lithium-Oxygen Batteries.

Li-O2 batteries provide a novel technology for electric energy storage due to their high energy density. However, the strong solvent coordination with Li+ at low temperatures impacts their performance and triggers irreversible interfacial reactions on the Li anode. Herein, cyclopentyl methyl ether (CME) is incorporated in a dimethoxyethane (DME)-based electrolyte to realize an anionic solvation transition at low temperatures in Li-O2 batteries. CME featuring a single O coordination site substitutes highly solvating DME in the first solvation sheath, and it induces more anion coordination to Li+ across the room- and low-temperature ranges. The low residence time of CME (66 ps at 25 °C, 382 ps at -40 °C.) in the solvation structures leads to the fast exchange of coordinated CME molecules with Li+ in comparison with DME and facilitates Li+ desolvation at low temperatures. The simultaneously generated inorganic-rich solid electrolyte interphase promotes Li+ transport to improve Li deposition and suppress Li dendrite formation. These enable the Li-O2 battery to present a good cycling stability of 110 cycles with a fixed capacity of 1000 mA h g-1 at -40 °C. This work paves the way for designing novel electrolytes in low-temperature batteries.

Insights into performance and mechanism of CuCo2O4/MXene composite as an efficient peroxymonosulfate activator for p-nitrophenol degradation

The slow redox cycle during oxidation and the toxicity risk due to leaching of metal ions greatly hinder the practical application of transition metal-based catalysts in advanced oxidation processes. In this study, a novel layered-structured CuCo2O4/MXene composite was prepared by self-assembling CuCo2O4 spinel with two-dimensional MXene and used as a heterogeneous catalyst based on peroxymonosulfate (PMS) activation for the oxidative degradation of p-nitrophenol (4-NP). Benefiting from its unique structure, the degradation efficiency of 4-NP by CuCo2O4/MXene-activated PMS was close to 100 % within 10 min at the initial pH 7.0. Quenching experiments and electron paramagnetic resonance demonstrated that both radical (SO4•- and •OH) and non-radical (1O2) pathways were involved in the degradation of 4-NP. X-ray photoelectron spectroscopy analysis showed that the introduction of MXene could significantly promote the efficient redox cycling of Cu2+/Cu+ and Co3+/Co2+ on the catalyst surface, thus providing a continuous driving force for the PMS activation. More importantly, CuCo2O4/MXene exhibited good ionic interference resistance, high stability and low metal ion leaching rate, suggesting its potential practical application. This work not only demonstrates that CuCo2O4/MXene is a promising catalyst for the degradation of organic pollutants by PMS activation, but also provides a new idea for the development of efficient PMS-activated catalysts for pollutant degradation.

A novel formulation of an eco-friendly calcium nitrate-based heavy completion fluid

Calcium-nitrate-based transparent completion fluids are widely used in the oil and gas industry for well completion and stimulation operations in carbonate reservoirs. These fluids have many advantages, such as medium density, low corrosion, good temperature stability, low clay swelling, and high wettability. However, the density of calcium nitrate brine is limited by its solubility, which can be increased by the addition of alcohols. This study investigated the effects of adding different types of alcohols (G1, G2, and G3) to calcium nitrate brine on the properties and performance of the completion fluid for carbonate reservoirs. The fluid properties and performance were evaluated through a series of laboratory tests, including density measurement, corrosion test, viscosity measurement, rheology test, temperature stability test, clay swelling test, wettability test, and compatibility test. The results showed that the addition of alcohols to brine can improve or reduce the fluid density, viscosity, corrosion, temperature stability, clay swelling, wettability, and compatibility, depending on the type and amount of alcohols. The optimum fluids have been selected based on their highest density, lowest viscosity, lowest corrosion, highest temperature stability, lowest clay swelling, highest wettability change, and highest compatibility with formation fluids. The densities of the fluids CN4, CNG14, CNG24, and CNG34 were respectively 96, 101, 101.5, and 100 pounds per cubic foot (pcf). Their rates of corrosion on L80 steel were respectively 0.829, 0.589, 0.720, and 0.599 mils per year (mpy). Their apparent viscosities at 62.4 °F were respectively around 15, 120, 100, and 60 centipoise (cp). Their apparent viscosities at 176 °F were all around 10 to 25 mpy. The fluids remained clear with no evidence of suspended solid particles at 20 °F, indicating their resilience to low-temperature conditions and suitability for use in cold weather operations. The fluid CNG24 becomes cloudy at 285 °F, and the fluid CNG34 becomes cloudy from 212 °F onward, but the CN4 fluid remains clear and transparent at all temperatures. In the tested high temperatures, the effect on their pH was around the same. The fluids CN4, CNG24, and CNG34 had a lower swelling index than 5 ml per 2 g of bentonite clay. The contact angles of oil and carbonate-type thin sections after their wettabilities were affected by the fluids were also 99.5, 36.54, and 46.03°, respectively. Finally, they’re all relatively compatible with formation fluids. The best completion fluid for carbonate reservoirs was CNG24, which contained calcium nitrate and G2 alcohol. This fluid had the best overall performance and safety of the fluids tested.

Dynamic mechanism and structural property analysis of microfluidic-obtained pea protein fiber

Although protein-based fibers have been developed, the preparation of inexpensive, safe and environmentally friendly plant protein fibers is still a hot topic. Herein, we constructed the microfluidic chip to produce protein fiber using pea protein and a small amount of cellulose nanofibers. The results demonstrated that compared to pea protein alone, the pea protein-based fiber produced through microfluidic spinning exhibited a smooth and dense surface structure, good thermal stability, higher Zeta potential and antioxidant activity, and lower surface hydrophobicity and sensitization. Furthermore, we comprehensively revealed the dynamic formation mechanism of pea protein-based fiber. During the fiber preparation process, hydrodynamics led the protein and cellulose nanofibers to align gradually along the flow direction, enhancing their molecular orientation. Additionally, strong hydrodynamic shear force altered the protein structure, transitioning its spatial structure from random coil and α-helix to β-sheet, and promoting a binding of protein to cellulose nanofibers. This research may offer theoretical guidance for the application of pea protein-based fiber in the preparation of hypoallergenic protein-based fibers and the construction of foodborne delivery materials.

Folic acid-assisted in situ solvothermal synthesis of Ni-MOF/MXene composite for high-performance supercapacitors

The synthesis of Ni-based metal-organic framework (MOF) and Ti3C2Tx MXene nanosheets is achieved via a straightforward solvothermal method, resulting in the formation of Ni-MOF/MXene composite material. This study introduces an innovative strategy that employs the biomolecule folic acid for the solvothermal synthesis of Ni-MOF/MXene nanosheets, intending to achieve high-performance supercapacitors. This approach effectively prevents the oxidation and restacking of MXene nanosheets and ensures the uniform dispersion of Ni-MOF on the surface of MXene nanosheets. The Ni-MOF/MXene composite exhibits an outstanding specific capacitance of 716.19 F/g at 1 A/g current density. Furthermore, an asymmetric supercapacitor device was assembled using activated carbon and Ni-MOF/MXene composite as negative and positive electrodes, respectively. The asymmetric device exhibited an impressive energy density of 23.28 Wh/kg at a power density of 2.841 KW/kg, along with good cyclic stability. These results establish an excellent potential of the Ni-MOF/MXene composite material as a candidate for next-generation energy devices.

Persistently boosted TEA sensing performance of In2O3 hollow spheres by sequentially modified with Bi2O3 and Pt nanoparticles

Exploring rational and feasible strategies to boost the gas sensing performance of metal oxide semiconductor (MOS) has attracted a growing concern because it is an inevitable path for advanced gas sensor. Herein, a Bi2O3 and Pt co-modification approach was proposed to upgrade the triethylamine (TEA) sensing performance of In2O3. To expound it, a series of hollow spheres (HSs) with different compositions including In2O3, Bi2O3-modified In2O3 (Bi2O3/In2O3), and Bi2O3 and Pt co-modified In2O3 (Pt/Bi2O3/In2O3) were prepared through a solvothermal-calcination route combined with subsequent chemical reduction. After the evolution from In2O3 to binary Bi2O3/In2O3 and ternary Pt/Bi2O3/In2O3 composites, the sensor exhibited a persistently boosted sensing performance to TEA, particularly of higher sensitivity (increased from 0.0219 to 0.0369 and 1.0319/ppm within 1–30 ppm), better selectivity (STEA/Methane increased from 17.6 to 23.7 and 257.2), and faster response speed (response time decreased from 7 to 3 and 1 s for 100 ppm TEA). Additionally, the best Pt/Bi2O3/In2O3 sensor also presented good repeatability and stability. The sensitization effects of Pt and Bi2O3-In2O3 p-n heterojunction were discussed in detail.

A thermally stable narrow-band green phosphor Na2MgAl10O17: Eu2+, Mn2+ for NUV LED application

Thermally stable narrow-band green phosphor is important for phosphor-converted light emitting diodes (pc-LEDs). Herein, an efficient and stable Na2MgAl10O17: Eu2+, Mn2+ green phosphor is obtained using Eu2+-Mn2+ energy transfer strategy. The Mn2+ activator could be effectively sensitized by the addition of Eu2+ with significant UV absorption enhancement, then generates a bright green emission with peak at 515 nm and FWHM of 40 nm. The internal and external quantum efficiencies of optimal phosphor Na2MgAl10O17: 0.15Eu2+, 0.50Mn2+ are measured as 69.5 and 30.8 %, respectively. It is found that Na2MgAl10O17: Eu2+, Mn2+ phosphor shows good chemical and thermal stability, i.e. maintains no quenching after H2O/HCl soaking and keeps 90 % of RT intensity at 200 °C. By using Na2MgAl10O17: Eu2+, Mn2+ as converted phosphor, single-chromatic and white pc-LEDs were fabricated, which simultaneously achieve green and white emission with high brightness. Our results show that this Na2MgAl10O17: Eu2+, Mn2+ is a potential candidate as a thermally stable green phosphor for white LEDs.

Synthesis of MoS2@MoO3/(Cu+/g-C3N4) ternary composites with double S-scheme heterojunction for peroxymonosulfate activation exposing to visible light

The construction of semiconductor heterojunction is an effective way for charge separation in photocatalytic degradation of pollutants. In this study, a novel MoS2@MoO3/(Cu+/g-C3N4) ternary composites (MMCCN) was prepared via a simple calcination method. The as-prepared composites exhibited exceptional performance in activating peroxymonosulfate (PMS) for the degradation of rhodamine B (RhB). The activity testing results indicated that 99.41 % of RhB (10 mg·L−1, 10 mL) was effectively removed by the synergistic effect of composites photocatalyst (0.1 g·L−1) and PMS (0.1 g·L−1) under visible light irradiation for 40 min. Its reaction rate constant exceeded that of Cu+/g-C3N4, MoO3 and MoS2 by a factor of 3.56, 17.30 and 11.73 times, respectively. The crystal structure, band gap and density of states (DOS) of the semiconductors were calculated according to the density functional theory (DFT). Free radical trapping tests and electron spin resonance spectroscopy validated that 1O2, O2− and h+ are primary reactive species participating in the decomposition of RhB. The ternary composites demonstrated good stability and maintained excellent degradation efficiency even across four reaction cycles. Furthermore, the activation mechanism and the intermediates produced during the decomposition course of RhB by MMCCN/PMS/vis system were analyzed and elucidated. A double S-scheme heterojunctions was responsible for efficient separation of photo-induced electron-hole pairs. This work presents a novel method in the construction of double S-scheme heterojunctions for PMS activation which is expected to find wide applications in wastewater treatment and environmental remediation.

Three-dimensional gel network structure of agarose interlayer dispersed Pd nanoparticles in copper foam electrode for electrocatalytic degradation of doxycycline hydrochloride

In this study, we successfully prepared palladium/agarose/copper foam (Pd/AG/CF) composite electrodes by utilizing the three-dimensional network structure agarose (AG), a green material derived from biomass, and homogeneously immobilizing palladium (Pd) atoms on a copper foam (CF) substrate through a facile route. The electrode showed excellent performance in the electrocatalytic degradation of doxycycline (DOX), with a high DOX degradation rate of 92.19 % in 60 min. In-depth studies revealed that palladium can form metal-metal interactions with the CF substrates, which enhances the electron transfer on the catalyst surface. In addition, the introduction of agarose effectively prevented the agglomeration of palladium nanoparticles. In addition, the hydroxyl functional groups in the molecular structure of agarose facilitate interactions between water molecules and the electrode interface through the formation of hydrogen bonds, thereby further enhancing the efficiency of the electrocatalytic reaction. In addition to good stability and reusability. Microbial toxicity test results show that the degraded wastewater has minimal impact on the environment. Also, possible degradation pathways of DOX were explored in this study. Finally, a novel continuous flow reactor was designed, featuring a unique design that ensures full contact between wastewater and the composite electrodes, thereby achieving continuous and efficient treatment of antibiotic wastewater.

Molecular Modeling Studies of Similar Molecules to Selective Estrogen Receptor Degrader Elacestrant as Inhibitors of SARS-COV-2.

Coronavirus 2019 (COVID-19) is a disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) strain. Many anticancer compounds have been repurposed as effective anti-coronavirus agents and are currently in a clinical trial to be evaluated for treatment. Elacestrant is a novel selective estrogen receptor degrader (SERD). A fingerprint Tanimoto-based 2-dimensional similarity search was performed in the PubChem database using elacestrant as a prototype. The chemical compounds were downloaded, and virtual screening, molecular docking, and molecular dynamics were further used to identify the most active molecules in the binding pocket SARS-COV-2 main protease. Eight compounds with superior docking score, gscore, and glide binding energy were identified. Molecular dynamic simulations (MD) were performed at 100 ns to remove the false interactions between the receptor and the active ligands. The results showed that all the compounds displayed good stability. Further, the ADMET results showed that compounds CID58023104 was observed to be deemed a hit compound; hence, CID58023104 and could be optimize, derivatize, and explore for further development as an anti-coronavirus agent targeting SARS-COV-2 main protease.

Core-shell electrospun polycaprolactone nanofibers, loaded with rifampicin and coated with silver nanoparticles, for tissue engineering applications

In the field of tissue engineering, the use of core-shell fibers represents an advantageous approach to protect and finely tune the release of bioactive compounds with the aim to regulate their efficacy. In this work, core-shell electrospun polycaprolactone nanofiber-based membranes, loaded with rifampicin and coated with silver nanoparticles, were developed and characterized. The membranes are composed by randomly oriented nanofibers with a homogeneous diameter, as demonstrated by scanning electron microscopy (SEM). An air-plasma treatment was applied to increase the hydrophilicity of the membranes as confirmed by contact angle measurements. The rifampicin release from untreated and air-plasma treated membranes, evaluated by UV spectrophotometry, displayed a similar and constant over-time release profile, demonstrating that the air-plasma treatment does not degrade the rifampicin, loaded in the core region of the nanofibers. The presence and the distribution of silver nanoparticles on the nanofiber surface were investigated by SEM and Energy Dispersive Spectroscopy. Moreover, SEM imaging demonstrated that the produced membranes possess a good stability over time, in terms of structure maintenance. The developed membranes showed a good biocompatibility towards murine fibroblasts, human osteosarcoma cells and urotheliocytes, reveling the absence of cytotoxic effects. Moreover, doble-functionalized membranes inhibit the growth of E. coli and S. aureus. Thanks to the possibilities offered by the coaxial electrospinning, the membranes here proposed are promising for several tissue engineering applications.

Regulating Strain and Electronic Structure of Indium Tin Oxide Supported IrOx Electrocatalysts for Highly Efficient Oxygen Evolution Reaction in Acid.

The development of proton exchange membrane water electrolysis is a promising technology for hydrogen production, which has always been restricted by the slow kinetics of the oxygen evolution reaction (OER). Although IrOx is one of the benchmark acidic OER electrocatalysts, there are still challenges in designing highly active and stable Ir-based electrocatalysts for commercial application. Herein, a Ru-doped IrOx electrocatalyst with abundant twin boundaries (TB-Ru0.3Ir0.7Ox@ITO) is reported, employing indium tin oxide with high conductivity as the support material. Combing the TB-Ru0.3Ir0.7Ox nanoparticles with ITO support could expose more active sites and accelerate the electron transfer. The TB-Ru0.3Ir0.7Ox@ITO exhibits a low overpotential of 203 mV to achieve 10 mA cm-2 and a high mass activity of 854.45 A g-1noblemetal at 1.53 V vs RHE toward acidic OER, which exceeds most reported Ir-based OER catalysts. Moreover, improved long-term stability could be obtained, maintaining the reaction for over 110 h at 10 mA cm-2 with negligible deactivation. DFT calculations further reveal the activity enhancement mechanism, demonstrating the synergistic effects of Ru doping and strains on the optimization of the d-band center (εd) position and the adsorption free energy of oxygen intermediates. This work provides ideas to realize the trade-off between high catalytic activity and good stability for acidic OER electrocatalysts.

High-fidelity imaging of drug-induced acute gastritis by using a fluorescent and photoacoustic dual-modal probe with good stability in stomach acid

In consideration of deep tissue imaging and signal fidelity, fluorescent-photoacoustic (PA) dual-modal probes are much more desirable. However, dual-modal imaging of gastritis using molecular probes remains a challenge due to the harsh gastric acid environment in the stomach. Based on the positive correlation between gastritis and cell viscosity, stomach acid-stable and viscosity-activated probes could potentially diagnose gastritis. As a proof of concept, herein, a fluorescent and photoacoustic dual-modal probe (named WSP-1) is revealed for the imaging of drug-induced acute gastritis in vivo. WSP-1 exhibits viscosity-dependent fluorescence emission and photoacoustic signals. A rotatable C–C single bond is incorporated into the D-π-A structure of WSP-1, which could facilitate the formation of the twisted intramolecular charge transfer (TICT) state in a low-viscosity environment (weak fluorescence/PA signal) and the intramolecular charge transfer (ICT) state in a high-viscosity environment (strong fluorescence/PA signal). WSP-1 has demonstrated the capability to target mitochondria and can be utilized to monitor the viscosity enhancement of cells during inflammation. Most importantly, WSP-1 exhibits good optical and structural stability in gastric acid. By leveraging these desirable features of WSP-1, we have achieved fluorescent and 3D photoacoustic in situ imaging of drug-induced acute gastritis following oral administration of WSP-1.

Dual-Purpose Aptamer-Based Sensors for Real-Time, Multiplexable Monitoring of Metabolites in Cell Culture Media.

The availability of high-frequency, real-time measurements of the concentrations of specific metabolites in cell culture systems will enable a deeper understanding of cellular metabolism and facilitate the application of good laboratory practice standards in cell culture protocols. However, currently available approaches to this end either are constrained to single-time-point and single-parameter measurements or are limited in the range of detectable analytes. Electrochemical aptamer-based (EAB) biosensors have demonstrated utility in real-time monitoring of analytes in vivo in blood and tissues. Here, we characterize a pH-sensing capability of EAB sensors that is independent of the specific target analyte of the aptamer sequence. We applied this dual-purpose EAB to the continuous measurement of pH and phenylalanine in several in vitro cell culture settings. The miniature EAB sensor that we developed exhibits rapid response times, good stability, high repeatability, and biologically relevant sensitivity. We also developed and characterized a leak-free reference electrode that mitigates the potential cytotoxic effects of silver ions released from conventional reference electrodes. Using the resulting dual-purpose sensor, we performed hourly measurements of pH and phenylalanine concentrations in the medium superfusing cultured epithelial tumor cell lines (A549, MDA-MB-23) and a human fibroblast cell line (MRC-5) for periods of up to 72 h. Our scalable technology may be multiplexed for high-throughput monitoring of pH and multiple analytes in support of the broad metabolic qualification of microphysiological systems.

Amino‐Arsine and Amino‐Phosphine Based Synthesis of InAs@InP@ZnSe core@shell@shell Quantum Dots

AbstractA colloidal synthesis protocol is demonstrated for InAs@InP core@shell quantum dots (QDs) with a tunable InP shell thickness (ranging from 3 to 8 monolayers), utilizing tris(diethylamino)‐arsine and ‐phosphine. Structural analysis reveals that the InP shell preferentially grows onto the tetrahedral InAs cores along the <‐1‐1‐1> directions, forming tetrapodal‐shaped InAs@InP QDs. Growth of the InP shell causes a red shift in the absorption spectrum of the QDs. This is explained by considering that electrons are delocalized throughout the whole core@shell QDs, while holes preferentially leak along the <‐1‐1‐1> directions, as indicated by the density functional theory calculations. This means such heterostructures cannot be described as type‐I or quasi type‐II, contrary to earlier assumptions. The overlap of carrier wavefunctions throughout the entire InAs@InP QD structure results in no significant reduction of the Auger recombination rate, which remains as fast as in InAs QDs. However, the InP shell enhances photoluminescence (PL) efficiency (up to ≈13%) by passivating surface trap states of the InAs QDs (mainly located close to the top of the valence band). The overgrowth of a ZnSe shell endows the QDs with a high PL efficiency (≈55%) and good stability upon air exposure (≈80% PL intensity retention after 14 days).

Motion Sensing by a Highly Sensitive Nanogold Strain Sensor in a Biomimetic 3D Environment.

Recent advancements in flexible electronics have highlighted their potential in biomedical applications, primarily due to their human-friendly nature. This study introduces a new flexible electronic system designed for motion sensing in a biomimetic three-dimensional (3D) environment. The system features a self-healing gel matrix (chitosan-based hydrogel) that effectively mimics the dynamics of the extracellular matrix (ECM), and is integrated with a highly sensitive thin-film resistive strain sensor, which is fabricated by incorporating a cross-linked gold nanoparticle (GNP) thin film as the active conductive layer onto a biocompatible microphase-separated polyurethane (PU) substrate through a clean, rapid, and high-precision contact printing method. The GNP-PU strain sensor demonstrates high sensitivity (a gauge factor of ∼50), good stability, and waterproofing properties. The feasibility of detecting small motion was evaluated by sensing the beating of human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte spheroids embedded in the gel matrix. The integration of these components exemplifies a proof-of-concept for using flexible electronics comprising self-healing hydrogel and thin-film nanogold in cardiac sensing and offers promising insights into the development of next-generation biomimetic flexible electronic devices.

Vanadium Carbide Quantum Dots Exert Efficient Anti‐Inflammatory Effects in Lipopolysaccharide‐Induced BV2 Microglia and Mice

The regulation of glial cell activation is a critical step for the treatment or prevention of neuroinflammation‐based brain diseases. However, the development of therapeutic drugs that pass the blood–brain barrier (BBB) and inhibit the glia cell activation remains a significant challenge. Herein, an ultrasmall 2D vanadium carbide quantum dots (V2C QDs) that are capable of crossing the BBB are prepared, and the admirable anti‐neuroinflammatory effects are presented. The prepared 2D V2C QDs with an average size of 2.54 nm show good hydrophilicity, physiological stability, and effective BBB‐crossing ability. The biological effect of V2C QDs on inflammatory reactions demonstrates fascinating results in preventing the impairment of learning and memory in BALB/c mice stimulated by lipopolysaccharide. Investigation of molecular mechanism reveals that V2C QDs not only inhibit the toll‐like receptor 4/myeloid differentiation factor 88‐mediated nuclear factor kappa B and mitogen‐activated protein kinase pathways, but also prevent eukaryotic translation initiation factor 2α/activating transcription factor 4/C/EBP homologous protein‐signaling pathway and reduce oxidative stress via activating the NF‐E2‐related factor‐2/heme oxygenase‐1‐signaling pathway, leading to greatly inhibited activation of microglia and astrocytes and weakened production of inflammatory cytokines. In summary, V2C QDs exert potent anti‐inflammatory effects through multiple pathways, thus offer great potential for the treatment of neurodegenerative diseases.

Simultaneously blocking ANGPTL3 and IL-1β for the treatment of atherosclerosis through lipid-lowering and anti-inflammation.

Blood lipid levels play a critical role in the progression of atherosclerosis. However, even with adequate lipid reduction, significant residual cardiovascular risk remains. Therefore, it is necessary to seek novel therapeutic strategies for atherosclerosis that can not only lower lipid levels but also inhibit inflammation simultaneously. The fusion protein FD03-IL-1Ra was designed by linking the Angiopoietin-like 3 (ANGPTL3) nanobody and human interleukin-1 receptor antagonist (IL-1Ra) sequences to a mutated human immunoglobulin gamma 1 (IgG1) Fc. This construct was transfected into HEK293 cells for expression. The purity and thermal stability of the fusion protein were assessed using SDS-PAGE, SEC-HPLC, and differential scanning calorimetry. Binding affinities of the fusion protein to ANGPTL3 and IL-1 receptor were measured using Biacore T200. The biological activity of the fusion protein was validated through in vitro experiments. The therapeutic efficacy of the fusion protein was evaluated in an ApoE-/- mouse model of atherosclerosis, including serum lipid level determination, histological analysis of aorta and aortic sinus sections, and detection of inflammatory and oxidative stress markers. ImageJ software was utilized for quantitative image analysis. Statistical analysis was performed using one-way ANOVA followed by Bonferroni post hoc test. The FD03-IL-1Ra fusion protein was successfully expressed, with no polymer formation detected, and it demonstrated good thermal and conformational stability. High affinity for both murine and human ANGPTL3 was exhibited by FD03-IL-1Ra, and it was able to antagonize hANGPTL3's inhibition of LPL activity. FD03-IL-1Ra also showed high affinity for both murine and human IL-1R, inhibiting IL-6 expression in A549 cells induced by IL-1β stimulation, as well as suppressing IL-1β-induced activity inhibition in A375.S2 cells. Our study revealed that the fusion protein effectively lowered serum lipid levels and alleviated inflammatory responses in mice. Furthermore, the fusion protein enhanced plaque stability by increasing collagen content within atherosclerotic plaques. These findings highlighted the potential of bifunctional interleukin-1 receptor antagonist and ANGPTL3 antibody fusion proteins for ameliorating the progression of atherosclerosis, presenting a promising novel therapeutic approach targeting both inflammation and lipid levels.