Good Stability Research Articles

Physical, biochemical, and biological characterization of olive-derived lipid nanovesicles for drug delivery applications

AbstractExtracellular vesicles (EVs) have shown great promise as drug delivery system (DDS). However, their complex and costly production limit their development for clinical use. Interestingly, the plant kingdom can also produce EV-like nanovesicles that can easily be isolated and purified from a large quantity of raw material at a high yield. In this study, olive-derived nanovesicles (ODNVs) were isolated from raw fruits using serial centrifugations and their physical and biological features characterized to demonstrate their promising potential to be used as a DDS. Nanotracking particle analysis indicated an average size of 109.5 ± 3.0 nm and yield of 1012 ODNVs/mL for the purest fraction. Microscopy imaging, membrane fluidity assay and lipidomics analysis showed the presence of a rich lipid bilayer that significantly varied between different sources of ODNVs but showed a distinct signature compared to human EVs. Moreover, ODNVs were enriched in PEN1 and TET8 compared to raw fruits, suggesting an extracellular origin. Interestingly, ODNVs size and yield stayed unchanged after exposure to high temperature (70 °C for 1 h), wide pH range (5–10), and 50–100 nm extrusion, demonstrating high resistance to physical and chemical stresses. This high resistance allowed ODNVs to stay stable in water at 4 °C for a month, or with the addition of 25 mM trehalose for long-term freezing storage. Finally, ODNVs were internalized by both 2D and 3D cell culture without triggering significant cytotoxicity and immunogenicity. Importantly, the anticancer drug doxorubicin (dox) could be loaded by passive incubation within ODNVs and dox-loaded ODNVs decreased cell viability by 90% compared to only 70% for free dox at the same concentration, indicating a higher efficiency of drug delivery by ODNVs. In addition, this high cytotoxicity effect of dox-loaded ODNVs was shown to be stable after a 2-week storage at 4 °C. Together, these findings suggested that ODNVs represent a promising candidate as drug nanocarrier for various DDS clinical applications, as demonstrated by their biocompatibility, high resistance to stress, good stability in harsh environment, and improvement of anticancer drug efficacy.

Dynamic Self-Healing of the Reconstructed Phase in Perovskite Oxides for Efficient and Stable Electrocatalytic OER.

Neither electrocatalytic activity nor structural stability is inconsequential in water electrolysis. Unfortunately, they have to be compromised in practice, especially in the anodic redox chemistry of lattice oxygen. Herein, the discovery of a La1- xCexFeO3 perovskite is presented which shows both good stability and high catalytic activity. Using advanced operando characterizations, it is identified that the self-healing evolution of the La1- xCexFeO3 perovskite plays a key role during water oxidation in the lattice oxygen-mediated mechanism (LOM) pathway. Unlike irreversible reconstruction, the formation of reconstructed active-phase α-FeOOH is reversed by re-crystallization of surface La1- xCexFeO3 upon return to noncatalytic conditions. The self-healing transformation of the α-FeOOH termination layer on the stable La1- xCexFeO3 core imparts remarkable long-term stability as well as excellent electrocatalytic performance. As a result, a composition La0.9Ce0.1FeO3@FeOOH is designed that exhibits ultralow overpotentials of 257 and 312mV to achieve 10 and 100mAcm-2, respectively. The findings provide insight into self-healing behavior toward engineering perovskite oxides for efficient and stable oxygen electrocatalysis.

A dual-band broadband absorber using frequency selective surface for satellite antenna.

A dual-band broadband absorber based on frequency selective surface (FSS) is proposed. This double-layer absorber consists of two layers of FSS structure; each layer has a unique construction and plays its wave-absorbing function in different frequency bands. In the low-frequency band, layer I is a transmission channel, and layer II effectively absorbs electromagnetic waves. Furthermore, layer I exhibits significant absorption characteristics in the high-frequency band, whereas layer II provides effective reflection. The combination of the two layers results in a remarkably effective absorption. Its reflectivity below - 10dB covers a bandwidth of 5.5-14.8GHz and 22.9-33.1GHz, and its fractional bandwidth is 128%. The structure prevents external electromagnetic interference from affecting the antenna's performance, ensuring communication quality in critical frequency bands while reducing unwanted absorption in unwanted frequency bands. It is worth mentioning that the structure still has good stability under 45° oblique incidence. The physical mechanism is analyzed in detail by using an equivalent circuit model (ECM). The measurement results are in good agreement with those of simulation and ECM.

Addition of polyphenolic extracts of Myrtus communis and Arbutus unedo fruits to whey: valorization of a common dairy waste product as a functional food.

Whey, a nutrient-rich byproduct of the dairy sector, possesses high potential for creating novel nutraceutical products. The present study investigates a potential new functional food by incorporating polyphenolic extracts from Myrtus communis and Arbutus unedo fruits into whey in both liquid (LA) and powder (PA) addition forms. Chemical, microbiological, physical stability and antioxidant activity were monitored for 60 days (from T0 to T60). Both LA and PA of fruit extracts remained chemically stable for the whole period, except for A. unedo PA, which showed a decline in polyphenols after T45. Enriched whey samples showed higher antioxidant activity than pure whey. Microbiological analysis revealed the presence of lactic acid bacteria, indicating potential prebiotic effects. However, the high tannin concentration of A. unedo extracts partially modified the casein micelle structure. Whey enriched with Mediterranean fruit extracts shows great potential as a functional food, combining the benefits of plant antioxidants, probiotic bacteria and good stability. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Asymmetric Pore Windows in Pillar-Layered Metal-Organic Framework Membranes for H2/CO2 Separation.

In this study, a novel ultramicroporous pillar-layered Ni-LAP-NH2 [Ni2(l-asp)2(Pz-NH2)] (l-asp = l-aspartic acid, Pz-NH2 = aminopyrazine) membranes on porous α-Al2O3 tubes with high performance and good thermal stability was first fabricated using isostructural Ni-LAP[Ni2(l-asp)2(Pz)] (Pz = pyrazine) crystals as seeds. Utilizing the principle of reticular chemistry, here, we introduced the active amino side group into the Ni-LAP frameworks by replacing the pillar-layered ligand Pz with Pz -NH2 while maintaining the original Ni-LAP small pore size, and the amino side group induced a "steric hindrance" effect and the physical adsorption affinity, which synergistically delayed CO2 penetration. It was found that the preferential (111) orientation Ni-LAP-NH2 membrane (Z10) exhibited a high H2/CO2 separation performance with a separation factor of 41.7 and H2 permeance of 9.08 × 10-8 mol·m-2·s-1·Pa-1 under optimal conditions. These MOF materials demonstrated potential for industrial H2 purification due to their tunable pore structure and remarkable stability. Moreover, this strategy offers an effective approach to tailoring pillar-layered MOF membranes with targeted molecular sieving ability.

High-Performance Radiative Cooling Sunscreen.

Radiative cooling is a zero-energy-consumption cooling technology that shows great potential for outdoor human thermal management. To keep human skin comfortable in hot days, we herein develop a radiative cooling (RC) sunscreen that exhibits a low ultraviolet (UV) transmissivity (4.86%), a high solar reflectivity (90.19%), and a high mid-infrared emissivity (92.09%) to effectively provide both UV protection and skin cooling. As a result, the RC sunscreen exhibits a high cooling performance for decreasing the human skin temperature by 2.3-6.1 °C more than commercial sunscreens and 4.2-6.0 °C more than bare skin in a variety of outdoor scenarios in summer (e.g., low-humidity sunny days, high-humidity sunny days, and high-humidity cloudy days). In addition, the RC sunscreen also shows a good UV stability (12 h, 125 W), a high water resistance (106°), a long working life (30 days), and a good biocompatibility, thereby exhibiting promising commercial potentials in the sunscreen market.

Artificial Cascade Transformation Biosystem for One-Pot Biomanufacturing of Odd-Numbered Neoagarooligosaccharides and d-Tagatose through Wiser Agarose Utilization.

The application of agarose oligosaccharides has garnered great attention, with their biological activities varying among different structures. However, it still meets a great bottleneck for the targeted production of odd-numbered neoagarooligosaccharides (NAOSs), such as neoagarotriose (NA3), due to the lack of one-step hydrolases. In this work, the α-agarase AgaA33 and β-galactosidase BgaD were synergistically used to prepare NA3 with agarose as a substrate. Additionally, an l-arabinose isomerase CaLAI from Clostridium acetobutylicum was characterized to valorize low-value byproducts (d-galactose) by forming d-tagatose, which exhibited good thermal stability without the need for additional metal ions. Under the optimal reaction conditions, the production of NA3 and d-galactose catalyzed by these three enzymes was 0.40 and 0.15 g/L, respectively. The artificial three-enzyme-based cascade transformation system not only achieved the highest production of NA3 until now but also allowed for the valorization of d-galactose, providing a wiser application route for agarose utilization.

Recent Advances in Perovskite Nanomaterials for Sensing Applications

Abstract Perovskite-structured materials have been extensively studied for sensing applications on account of their good thermal stability and 3-4 eV bandgap. They have been utilized to detect traces of O2, NO, CO, NO2, CO2, H2O, CH4, H2 and similar smaller gas molecules such as C2H5OH etc. In addition, other sensing applications are optical sensing, I-V fluctuations, strain sensors, electromechanical sensors, sensing of metal ions, etc. Perovskite-structured halides are reported for sensing of metal ions, toxic gases, Volatile Organic Compounds (VOCs), detection of fungicides, pesticides, explosives, cellular imaging, radiation detection and humidity and temperature sensing.

A Ni4O4-cubane-squarate coordination framework for molecular recognition.

Molecular recognition is a fundamental function of natural systems that ensures biological activity. This is achieved through the sieving effect, host-guest interactions, or both in biological environments. Recent advancements in multifunctional proteins reveal a new dimension of functional organization that goes beyond single-function molecular recognition, emphasizing the need for artificial multifunctional materials in industrial applications. Herein, we have designed a porous Ni4O4-cubane squarate coordination polymer as an artificial molecular recognition host, drawing inspiration from the structural and functional features of natural enzymes. A comprehensive assessment of the material's ability to distinguish target species under different operating conditions was carried out. The results confirm its sieving function through hexane isomers separation, host-guest interaction function via xenon/krypton separation, and dual presence of sieving and interaction through carbon dioxide/nitrogen separation. Additionally, the material demonstrates good stability and feasibility for large-scale production, indicating its practical potential. Our findings provide a bio-inspired multifunctional recognition material for chemical separations as proof-of-concept while offering solutions to advance artificial multifunctional materials adaptable to other applications beyond chemical separations.

Formulation and Evaluation of Mucoadhesive Buccal Tablets of Carvedilol

The aim of the study was to formulate and evaluate the carvedilol buccal tablets using HPMC K4M and xanthan gum as polymer. Buccal tablets of carvedilol were prepared by direct compression technique by using polymer in combination at different concentration. Drug excipient compatibility study indicates that there is no interaction between the excipient and the drug. Total seven batches were prepared and subjected to evaluation parameters. Pre-compression parameters for all batches showed excellent flow propertied of powder bled. Prepared buccal tablets were evaluated for various post compression parameters like hardness, thickness, weight variation, drug content, and friability, % swelling index, muco-adhesive strength and in vitro drug release. The harness of all batches was optimum showed good mechanical strength; thickness of tablets was uniform in all formulations the weight variation test of all the formulation was found to be within the limits of pharmacopoeial standard. % swelling index for all the batch formulation was optimum and seen to increase with increase in polymer concentration. Mucoadhesive strength was also showed acceptable results for all batch formulations which satisfy the need of mucoadhesive tablets. The in vitro dissolution profile of all the formulation showed sustained release of drug, for extended periods of time. The optimized formulation of F4 prepared with was consider as the optimized formulation with respect to drug content, % swelling index, Mucoadhesive strength and in vitro drug release pattern for 8 hrs. Formulation F4 showed highest 98.32±1.55 % drug release at the end of 8 hrs. Optimized formulation F4 was found to be stable during the stability studies for 3 month indicating good stability of the formulation. Keywords: carvedilol, Mucoadhesive strength, Optimized formulation, buccal tablets.

Light-Activable Inhibitor Overcomes Antimicrobial Resistance and Regulates Antibacterial Activity.

Overuse of antibiotics and the widespread environmental accumulation of antibiotics drive the evolution and spread of antimicrobial resistance, posing a significant global health threat by reducing the effectiveness of available treatments and increasing the risk of untreatable infections. We designed and synthesized PhoPS, a novel photocaged β-lactamase inhibitor, which incorporates the pharmacophore of sulbactam caged with a photoresponsive moiety of o-nitrobiphenyl derivative. Experimental results demonstrate its rapid photoactivation, good stability in solution, and light-activated β-lactamase inhibition in vitro. PhoPS displays synergy with a cephalosporin antibiotic cefoperazone against both susceptible and resistant strains of Escherichia coli and biofilm formation. Additionally, PhoPS treatment demonstrates the potential to suppress the development of resistance in E. coli. These findings suggest that PhoPS offers a promising approach for restoring the efficacy of existing antibiotics and mitigating the emergence of AMR.

Jammed Pickering Emulsion Gels.

Emulsion gels with specific rheological properties have widespread applications in foods, cosmetics, and biomedicines. However, the constructions of water-in-oil emulsion gels are still challenging, due to the limited interactions available in the continuous oil phase. Here, a versatile strategy is developed to prepare a new type of emulsion gels, called Jammed Pickering emulsion gels (JPEGs). In the JPEG system, SiO2 NPs in the oil phase serve as colloidal surfactants to stabilize water-in-oil Pickering emulsions, while positively-charged NH2-PEG-NH2 molecules in the water phase cross-link negatively-charged SiO2 NPs at the water/oil interface, making NP-stabilized water droplets hard to deform and thus jamming the emulsion system to form emulsion gels. The strategy to prepare JPEGs is versatile and applicable to diverse oil phases. The designed JPEGs possess many advantages, including good biocompatibility for widespread applications, shear-thinning rheological properties for easy processing, good stability Over a wide temperature range and Against centrifugation, good adhesion to wet tissues for tissue engineering, and well-controlled sustained release Under intestinal conditions. The developed JPEGs are demonstrated to be a promising delivery platform and the strategy to achieve JPEGs will trigger more innovations of material design.

Mannose/stearyl chloride doubly functionalized polyethylenimine as a nucleic acid vaccine carrier to promote macrophage uptake

Transmembrane transport remains a significant challenge for nucleic acid vaccine vectors. Promoting the ability of immune cells, such as macrophages, to capture foreign stimuli is also an effective approach to improving cross-presentation. In addition, polyethyleneimine (PEI) has gained attention in the field of nucleic acid vaccine carriers due to its excellent gene transfection efficiency and unique proton buffering effect. However, although high molecular weight PEI exhibits high efficiency, its high-density positive charges make it highly toxic, which limits its application. In this study, mannose/stearyl chloride functionalized polyethylenimine (SA-Man-PEI) was prepared by functionalizing PEI (molecular weight of 25 kDa) with mannose with immunomodulatory and phagocyte targeting effects, and an alkyl hydrophobic chain segment, which could easily promote cell uptake. Moreover, the functionalized-PEI retains a strong proton buffering effect, which helps the carrier escape from the lysosome. The particle sizes of the composite particles formed by SA-Man-PEI and ovalbumin (OVA) were below 200 nm, with good storage stability at both 4 °C and 37 °C. At a drug concentration of 2 μg/mL, the cell survival rate of functionalized-PEI was 19.2% higher than that of unfunctionalized PEI. In vitro macrophage endocytosis experiments showed that SA-Man-PEI could significantly enhance the macrophage uptake of composite particles, compared to unfunctionalized PEI or single-functionalized PEI. This study offers a new approach for developing PEI as a nucleic acid vaccine carrier, which could simultaneously enhance cell targeting and promote cell uptake.

Surface Sulfurization of Cubic Cu2O to Form Cu2S Nanostructure-Decorated Catalysts for Light-enhanced Electrocatalytic H2 Evolution and Photocatalytic CO2 Reduction.

Developing highly efficient bifunctional catalysts for electrocatalysis and photocatalysis suffers from sluggish charge transfer and poor photo-to-electric conversion rate. Herein, micro-sized Cu2O cubes were initially prepared for subsequent transformation into Cu2O@Cu2S core-shell structures by a slight surface sulfurization. The formation of nanostructured Cu2S thin layer at Cu2O surface can endow an immediate charge separation and migration under light irradiation, leading to high surface photovoltages (SPV, 18-30 mV). As a result, the as-prepared Cu2O@Cu2S catalysts exhibited reduced overpotentials (η, only 86 mV is required for the optimized sample to achieve a current density of 10 mA cm-2) for H2 evolution with good cycling stability in electrocatalytic water splitting. Meanwhile, a high methane (CH4) production rate of 30.3 μmol h-1 g-1 can be delivered from the optimized Cu2O@Cu2S sample when used for photocatalytic CO2 reduction. This work has provided a strategy to prepare bifunctional catalysts with improved photoelectric properties for photo/electrocatalytic applications.

414.9 W in-band pumped Er/Yb co-doped fiber amplifier seeded by a random fiber laser.

We demonstrated a 414.9 W large-mode-area Er/Yb co-doped fiber amplifier with a good time-domain stability, seeded by a 1568 nm random fiber laser (RFL) seed with a half-open cavity. We believe this to be the highest output power of the RFL in a 1.5 µm band to date. At the maximum output power, the optical efficiency was 36.9% and the 3 dB linewidth was 0.69 nm. The 1535 nm fiber lasers were used as the pumping source of the main amplifier to avoid the parasitic lasing of Yb ions. The spectral linewidth broadening was suppressed during the amplification process due to the stable temporal output of the RFL seed. The M2 factor increased from 2.28 at 145.7 W to 2.83 at 280.8 W. Our research provides a robust approach for achieving high-power and high-spectral-purity RFLs in the 1.5 µm band.

Ultrasensitive, Fast-Response, and Stretchable Temperature Microsensor Based on a Stable Encapsulated Organohydrogel Film for Wearable Applications.

Ionic conductive hydrogel-based temperature sensors have emerged as promising candidates due to their good stretchability and biocompatibility. However, the unsatisfactory sensitivity, sluggish response/recovery speed, and poor environmental stability limit their applications for accurate long-term health monitoring and robot perception, especially in extreme environments. To address these concerns, here, the stretchable temperature sensors based on a double-side elastomer-encapsulated thin-film organohydrogel (DETO) architecture are proposed with impressive performance. It is found that the water-polyol binary solvent, organohydrogel film, and sandwiched device structure play important roles in the temperature sensing performance. By modifying the composition of binary solvent and thicknesses of organohydrogel and elastomer films, the DETO microsensors realize a thickness of only 380 μm, unprecedented temperature sensitivity (37.96%/°C), fast response time (6.01 s) and recovery time (10.53 s), wide detection range (25-95.7 °C), and good stretchability (40% strain), which are superior to those of conventional hydrogel-based sensors. Furthermore, the device displays good environmental stability with negligible dehydration and prolonged operation duration. With these attributes, the wearable sensor is exploited for the real-time monitoring of various physiological signals such as human skin temperature and respiration patterns as well as temperature perception for robots.

Engineering Densely Packed Ion-Cluster Electrolytes for Wide-Temperature Lithium-Sulfurized Polyacrylonitrile Batteries.

The electrolyte plays an essential role in the advancement of lithium-sulfur batteries (LSBs), as it not only transports the charge carriers but also extensively influences sulfur conversion mechanisms and electrode-electrolyte interphases formed on the electrode surface, thereby directly impacting battery performance. However, the majority of existing electrolytes suffer from incompatibility with either the Li anode or the sulfur cathode. Here, we develop a densely packed ion-cluster electrolyte (DPIE) through the strategic combination of a weakly solvating solvent and an inert diluent, resulting in the self-assembly of abundant compact ion-pair aggregates within its structure. This peculiar solvation structure promotes fast Li+ desolvation, the formation of robust electrode-electrolyte interphases, and the suppression of polysulfide dissolution. Leveraging the tailored DPIE, room-temperature Li||sulfurized polyacrylonitrile (SPAN) batteries demonstrate 300 stable cycles with a capacity retention of 97.8% and a steady Coulombic efficiency exceeding 99.9%. Even under a limited negative/positive areal capacity ratio of four, the Li||SPAN cells exhibit good stability over 250 cycles with 97.1% capacity retention. Furthermore, Li||SPAN batteries show impressive stability over a wide temperature range spanning from -20 to 60 °C and exhibit reversibility at -10 °C over 200 cycles. This electrolyte design enables LSBs with prolonged operational lifetimes, rapid charging capabilities, and expanded temperature tolerance.

Hydrovoltaic-Photoelectric Coupling Strategy Triggered a Robust Output Signal for High-Performance Self-Powered Electrochemical Sensing.

Hydrovoltaic self-powered electrochemical sensors hold significant potential for constructing wearable, portable, and real-time detection devices, but the low output signal due to the slow phase transition rate of water molecules and the intricate nature of integration limits their applications. In this work, a hydrovoltaic-photovoltaic coupling effect-enhanced self-powered electrochemical sensor was prepared by combining zinc oxide (ZnO) nanowire arrays with cerium-organometallic framework (Ce-MOF) materials, which greatly improved the electrical output of self-powered electrochemical systems and provided a new detection strategy for an efficient self-powered electrochemical sensing system. The heterojunction constructed by ZnO arrays and Ce-MOF could generate a built-in electric field under the action of light irradiation and promote the separation of the photocarriers. Moreover, the number of charged particles in the film further boosted the water evaporation effect. Notably, the optimal ZnO/Ce-MOF-based self-powered electrochemical device by hydrovoltaic-photoelectric coupling strategy displayed an outstanding output signal, which was 11-fold that of a pure hydrovoltaic-based device. As a proof of concept, the self-powered electrochemical sensing platform was explored for sensitive detection of lincomycin via electrostatic adsorption for the binding of an aptamer. The self-powered sensor showed superior performances, including a wide linear range from 1 fM to 1 nM with a detection limit of 0.2 fM, good stability, and satisfactory recoveries for the determination of lincomycin in real samples, holding great promise in environmental monitoring and food analysis. This study provides a promising avenue to boost the energy conversion efficiency with a high output signal for constructing sensitive self-powered biosensors.

Isolation and Characterization of a Novel Escherichia Bacteriophage with Potential to Control Multidrug-Resistant Avian Pathogenic Escherichia coli and Biofilms

Background/Objectives: Avian pathogenic Escherichia coli (APEC) infection is a significant problem for the global chicken industry, as it decreases animal welfare and is associated with substantial economic losses. Traditionally, APEC infections have been controlled through the use of antibiotics, which has led to an increased prevalence of antibiotic-resistant E. coli. Therefore, developing alternative treatments for APEC infection is crucial. Methods: In this study, an Escherichia phage specific to multidrug-resistant (MDR) APEC, designated as phage vB_EcoP_PW8 (phage vECPW8), was isolated. The morphology, phage adsorption to host cells, one-step growth curve, thermal stability, pH stability, whole-genome sequencing, antibacterial ability, and antibiofilm efficacy of phage vECPW8 were evaluated. Results: The results demonstrated that phage vECPW8 has a Podoviridae morphology and is effective at lysing bacteria. Phage vECPW8 exhibited a high absorption rate to bacterial cells (more than 85% within 10 min) and had a latent period of 20 min, with a burst size of 143 plaque-forming units per cell. Additionally, phage vECPW8 showed good temperature and pH stability. The phage displayed strong antibacterial activity in vitro, and its efficacy in controlling bacteria was confirmed through scanning electron microscopy. Whole-genome sequencing revealed that the phage has a linear genome with 69,579 base pairs. The genome analysis supported the safety of the phage, as no toxin, virulence, or resistance-related genes were detected. Phage vECPW8 was identified as a novel lytic phage in the Gamaleyavirus genus and Schitoviridae family. The phage also demonstrated antibiofilm efficacy by reducing and preventing biofilm formation, as evidenced by biofilm biomass and bacterial cell viability measurements. Conclusions: These results indicate that phage vECPW8 is a promising candidate for the effective treatment of MDR APEC infections in poultry.

Nanotechnology-Driven Delivery of Caffeine Using Ultradeformable Liposomes-Coated Hollow Mesoporous Silica Nanoparticles for Enhanced Follicular Delivery and Treatment of Androgenetic Alopecia

Androgenetic alopecia (AGA) is caused by the impact of dihydrotestosterone (DHT) on hair follicles, leading to progressive hair loss in men and women. In this study, we developed caffeine-loaded hollow mesoporous silica nanoparticles coated with ultradeformable liposomes (ULp-Caf@HMSNs) to enhance caffeine delivery to hair follicles. Caffeine, known to inhibit DHT formation, faces challenges in skin penetration due to its hydrophilic nature. We investigated caffeine encapsulated in liposomes, hollow mesoporous silica nanoparticles (HMSNs), and ultradeformable liposome-coated HMSNs to optimize drug delivery and release. For ultradeformable liposomes (ULs), the amount of polysorbate 20 and polysorbate 80 was varied. TEM images confirmed the mesoporous shell and hollow core structure of HMSNs, with a shell thickness of 25–35 nm and a hollow space of 80–100 nm. SEM and TEM analysis showed particle sizes ranging from 140–160 nm. Thermal stability tests showed that HMSNs coated with ULs exhibited a Td10 value of 325 °C and 70% residue ash, indicating good thermal stability. Caffeine release experiments indicated that the highest release occurred in caffeine-loaded HMSNs without a liposome coating. In contrast, systems incorporating ULp-Caf@HMSNs exhibited slower release rates, attributable to the dual encapsulation mechanism. Confocal laser scanning microscopy revealed that ULs-coated particles penetrated deeper into the skin than non-liposome particles. MTT assays confirmed the non-cytotoxicity of all HMSN concentrations to human follicle dermal papilla cells (HFDPCs). ULp-Caf@HMSNs promoted better cell viability than pure caffeine or caffeine-loaded HMSNs, highlighting enhanced biocompatibility without increased toxicity. Additionally, ULp-Caf@HMSNs effectively reduced ROS levels in DHT-damaged HFDPCs, suggesting they are promising alternatives to minoxidil for promoting hair follicle growth and reducing hair loss without increasing oxidative stress. This system shows promise for treating AGA.