Flexible conductive hydrogels have great potential for healthcare and human motion sensing. However, it is difficult to simultaneously achieve conductive hydrogel epidermal sensors with reliable adhesion capabilities and excellent sensing properties, as well as accelerated wound healing performance in wearable hydrogels. Here, an epidermal sensor with excellent adhesion (0.6 kPa) and tensile strain (218.0 %) properties was assembled from an easy-to-prepare bilayer antimicrobial hydrogel, which effectively accelerates wound healing, as well as for human motion sensing. The upper hydrogel layer was composed of PVA, which could effectively enhance the mechanical properties of the bilayer hydrogel. The lower hydrogel layer consisted of polyacrylamide (PAm) and chitosan-dopamine (CC-DA). PAm with good adhesion properties adhered effectively to the skin surface. CC-DA not only had adhesion properties, but also has good antibacterial effects. It inhibited the growth of bacteria, which assisted in wound healing and infection prevention. Therefore, the design of the bilayer hydrogel combined the mechanical enhancement of PVA with the adhesion properties and antimicrobial effect of PAm and CC-DA to provide better wound repair. In addition, the double-layer hydrogel with good electrical conductivity (1.65 S·m−1) could sensitively monitor the tiny electrophysiological signals emitted by the human body during exercise rehabilitation training.