Double-layer hybrid nanofiber membranes by electrospinning with sustained antibacterial activity for wound healing

Abstract

Bacterial infection is a general problem that increases the burden of wound treatment. It has been intensely expected to fabricate bioactive wound dressings with good antibacterial effect. In this work, double-layer hybrid nanofiber membranes (DHNM) of PVA/COL@ZnONPs and PCL/Gt@TiO2NPs were built by continuous electrospinning. The matrix membranes with acceptable tensile strength and moisture permeability property were obtained by optimizing the formulation and spinning conditions. The matrix membranes exhibited Young’s modulus of 91.93 MPa and tensile stress of 2.61 MPa. The water vapor transmittance rate (WVTR) of matrix membranes was 1140.45 g/m2·day. ZnONPs in the inner layer were released quickly in 2 hours to destroy the bacteria in the wound. TiO2NPs in the outer layer were sustainably released within 7 days to resist the bacterial attack outsides. The double-layer membranes showed good biocompatibility and enhanced the migration of cells. The cell migration rate of double-layer hybrid membranes (DHNM) was higher than that of single layer membranes. The double-layer membrane showed 93% elimination of the wound specific P. aeruginosa, over 99% inhibition to E. coli and over 97% to S. aureus. This study provides a potential approach for wound dressings to improve antibacterial effect.