The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.