Potential of methyl fernesoate isolate from the Cyprus extract in prospective broodstock of shrimp (Penaeus monodon. Fab)

Abstract

Cyprus rhizoma is reported to have methyl fernesoate compounds that are identical to Juvenil hormone III, some researchers have used it as a natural pesticide to control insect populations, whereas in aquaculture, especially in crustacea Juvenil hormone (methyl fernesoate) can stimulate the activity of other hormones such as ecdysterone and serotonin which play a role in the regulation of metamorphosis and maturation of both male and female gonads. The research activities carried out consisted of two stages including isolation of methyl fernesoate compounds from rhizomes of Cyprus sp through extraction, maceration, and purification of these compounds using appropriate organic solvents, then the application of Cyprus sp extracts to prospective black tiger shrimp through injections at a dose of 0,100, and 150 ug / g BW, and ablation of the eyestalk as a positive control. The study was conducted using a completely randomized design (CRD) method with 5 treatments and 2 replications. Observations made include molting frequency, gonadal maturity level, number and diameter of eggs, and measurement of the rate of development of vitellogenesis through a molecular approach with RT-PCR technique using primary vitellogenin F, R and β-Actin F, R. The observation of molting frequency showed the highest molting frequency in the treatment of 150 ug / g BW that is 15 times during maintenance, while the parent shrimp that had the highest gonad maturity was in the treatment of 100 ug / g BW as much as 75%. The results of the measurement of the rate of development of vitellogenesis through a molecular approach with PCR technique using the vitellogenin primers F, R, and β-Actin F, R showed a positive development in the broodstock shrimp treated with Cyprus sp.