Rumen Of Goats Research Articles

The effectiveness of banana tuber and goat rumen as bio activator of liquid biopesticide fertilizers

The purpose of this study was to examine the use of bio-activator from banana tuber and goat rumen to make liquid biopesticide fertilizers. The liquid biopesticide fertilizer (LBF) was formulated from ginger, turmeric, curcuma, papaya and pineapple. The ingredients are blended prior to test. Treatment 1 (Bio) was set with the mixture of LBF added with bio-activator from banana tuber and goat rumen, the positive control mixture was added to EM4 bio activator, while control (C) only added with arcades. All treatments were mixed a concentration of bio-activator of 50 ppm. The results showed that the use of bio-activators from banana tubers and rumen goats to make liquid biopesticide fertilizers has shown effectiveness compared to controls. This process shows an increase in nitrogen content from day 13 to 17. Organic matter and carbon content are highest on day 13, while phosphorus content is highest on day 17.

CILIATE PROTOZOA POLYPLASTRON MULTIVESICULATUM FROM THE RUMEN OF INDIAN GOAT CAPRA HIRCUS (L.)

An investigation has been made to observe the morphology of rumen ciliate protozoa. A total 458 rumen fluid samples of the Indian goat Capra hircus were collected for one year. The study reveals that occurrence of Polyplastron multivesiculatum (Dogiel 1925). The body dimensions and variations of characters recorded from the specimens taken (n=20) at random from different slides and compared with earlier findings and critical comments were made on their specific identity. The presence of P.multivesiculatum in the rumen of goat is the new record in Maharashtra (India).

Maturation of the Goat Rumen Microbiota Involves Three Stages of Microbial Colonization.

Simple SummaryConsiderable attention has recently been focused on the rumen microbiome, which has been implicated in regulating a ruminant’s nutrient metabolism. From birth onwards, the colonization of the rumen microbial community is thus of crucial importance for growth and fiber digestion of goats. In this study, we have provided details of the progression of changes and colonization of ruminal bacteria and fungi before weaning. We have also predicted the molecular functions of the bacterial microbiota using CowPi. Our finding confirmed that maturation of the goat rumen microbiota involves three stages of core microbial colonization. The study of rumen microbial of young ruminants will benefit the optimization of feeding strategies to promote the development and digestion of a healthy rumen microbiota in later life.With increasing age, the rumen microbiota of new-born ruminants become central in the translation of fibrous feed substances into essential nutrients. However, the colonization process of the microbial community (especially fungal community) remains poorly understood in ruminants at pre-weaning stages. In this study, the rumen bacterial and fungal colonization processes were investigated in goats at eight stages using amplicon sequencing. For bacteria, we found 36 common core genera at D0, D3, D14, D28, and D56, including mainly Bacillus, Alloprevotella, Bacteroides, Prevotella_1, Lactococcus, and Ruminococcaceae_NK4A214. Firmicutes was the dominant phylum among the total microbiota in newborn goat kids (prior to nursing), while Bacillus, Lactococcus, and Pseudomonas were predominant genera. Interestingly, the proportion of Bacillus was as high as 55% in newborn animals. After milk nursing, the predominant phylum changed to Bacteroidetes, while the proportion of Bacillus and Lactobacillus was very low. CowPi was used to predict the functional gene pathways and we found increases in the abundance of genes associated with amino acid related enzymes, DNA repair and recombination proteins, aminoacyl tRNA biosynthesis, and peptidases after D3. With regard to fungi, we found that there were 51 common genera at day 0 (D0), D3, D14, D28, and D56, including mainly Cryptococcus, Aspergillus, and Caecomyces. Aspergillus occupied approximately 47% at day 0, but then it decreased from day 3 to day 14. This study indicates that the core microbes of rumen emerged shortly after birth, but the abundance was very different from the core genus of the adult rumen. In addition, we also report a detailed scheme of the bacterial and fungal colonization process in rumens and propose three distinct stages during the rumen colonization process in pre-weaning goats, which will offer a reference for the development of milk substitutes for small ruminants.

Corn oil supplementation enhances hydrogen use for biohydrogenation, inhibits methanogenesis, and alters fermentation pathways and the microbial community in the rumen of goats

Enteric methane (CH4) emissions are not only an important source of greenhouse gases but also a loss of dietary energy in livestock. Corn oil (CO) is rich in unsaturated fatty acid with >50% PUFA, which may enhance ruminal biohydrogenation of unsaturated fatty acids, leading to changes in ruminal H2 metabolism and methanogenesis. The objective of this study was to investigate the effect of CO supplementation of a diet on CH4 emissions, nutrient digestibility, ruminal dissolved gases, fermentation, and microbiota in goats. Six female goats were used in a crossover design with two dietary treatments, which included control and CO supplementation (30 g/kg DM basis). CO supplementation did not alter total-tract organic matter digestibility or populations of predominant ruminal fibrolytic microorganisms (protozoa, fungi, Ruminococcus albus, Ruminococcus flavefaciens, and Fibrobacter succinogenes), but reduced enteric CH4 emissions (g/kg DMI, -15.1%, P = 0.003). CO supplementation decreased ruminal dissolved hydrogen (dH2, P < 0.001) and dissolved CH4 (P < 0.001) concentrations, proportions of total unsaturated fatty acids (P < 0.001) and propionate (P = 0.015), and increased proportions of total SFAs (P < 0.001) and acetate (P < 0.001), and acetate to propionate ratio (P = 0.038) in rumen fluid. CO supplementation decreased relative abundance of family Bacteroidales_BS11_gut_group (P = 0.032), increased relative abundance of family Rikenellaceae (P = 0.021) and Lachnospiraceae (P = 0.025), and tended to increase relative abundance of genus Butyrivibrio_2 (P = 0.06). Relative abundance (P = 0.09) and 16S rRNA gene copies (P = 0.043) of order Methanomicrobiales, and relative abundance of genus Methanomicrobium (P = 0.09) also decreased with CO supplementation, but relative abundance (P = 0.012) and 16S rRNA gene copies (P = 0.08) of genus Methanobrevibacter increased. In summary, CO supplementation increased rumen biohydrogenatation by facilitating growth of biohydrogenating bacteria of family Lachnospiraceae and genus Butyrivibrio_2 and may have enhanced reductive acetogenesis by facilitating growth of family Lachnospiraceae. In conclusion, dietary supplementation of CO led to a shift of fermentation pathways that enhanced acetate production and decreased rumen dH2 concentration and CH4 emissions.

Studies on Digestion Coefficients and Rumen Fermentation of some Improved Rations by Males of Sinai Sheep and Goats

In order to improve the productivity of Sinai sheep and goats, the effects of feeding with mineral mixture additives (experiment 1) and the mixture of mineral with alfalfa (experiment 2) on the digestion coefficients (dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), ether extract (EE) and nitrogen free extract (NFE)), nutritive values (total digestible nutrients (TDN) and digestible crude protein (DCP)) and rumen fermentation were evaluated. Experiment 1 was applied to evaluate two rations (1 and 2). Ration 1 contained concentrate feed mixture (CFM) and wheat straw (ratio 2:1) and ration 2 contained CFM with additive of 0.2% mineral mixture and wheat straw. Experiment 2 was applied to evaluate two rations (A and B). Ration A contained CFM, Alfalfa and wheat straw and ration B contained CFM mixed with 0.2% mineral mixture additive, Alfalfa and wheat straw. Three Sinai rams and three Sinai bucks aged 2-3 years were used. The rumen parameters as pH, ammonia-N, Volatile fatty acids, microbial protein and protozoa were measured in rumen fluid at 0, 2 and 4 hours after feeding. Results of experiment 1 indicated that the differences of digestion coefficients of nutrients between ration 1 and ration 2 were not significant, while the EE digestibility in ration 2 (55.6%) was significantly higher (P<0.05) than ration1 (53.3%) in the sheep. The digestion coefficients of DM (65.1%) and OM (67.1%) in ration 2 were significantly higher (P<0.05) than ration 1 (62.5% and 64.1%, respectively) in the goats. The digestion coefficients of CF (58.6%) and EE (58.5%) of ration B were significantly higher (P<0.05) than ration A (56.5% and 56.2%, respectively) in the sheep. The digestion coefficients of DM (67.4%) and OM (68.6%) in ration B were significantly higher (P<0.05) than ration A (65.7% and 67.1%, respectively) in the goats. In sheep, NFE of ration B was significantly (P<0.05) lower than ration A (70.4% and 72.9%, respectively). In goats, TDN values were significantly (P<0.05) higher in ration 2 than ration 1 (64.3% and 63.1%, respectively) and also in ration B than ration A (64.9% and 62.4%, respectively). The difference of NH3-N at 4h post feeding in rumen of goats was significantly (P<0.05) lower in ration 2 than ration 1 (17.7 mg and 21mg per 100 ml rumen liquid, respectively). The lowest values (P<0.05) of pH were recorded at 4 h after feeding in both breeds. In sheep, microbial protein (MP) in the rumen fluid was significantly (P<0.05) higher in ration B than ration A (0.90 g and 0.84 g per 100 ml rumen liquid, respectively). It could be concluded that using of the mixture of mineral additives (N.Candles© Pharm) in feeding Sinai sheep and goats can improve the digestion coefficients, nutritive values and rumen fermentation. Further studies in this area are recommended to evaluate effect of mineral additives on animal performance and its economical return.

Age-associated changes of the intrinsic nervous system in relation with interstitial cells in the pre-weaning goat rumen.

In this study, we investigated the neural changes and their relationships with interstitial cells (ICs) in the rumen of pre-weaning goats by transmission electron microscopy, western blot and immunofluorescence (antibody: general neuronal marker-Protein Gene Product (PGP9.5)/ IC marker-vimentin). The immunofluorescence results showed that PGP9.5-positive reaction was widely distributed in neuronal soma (NS) and nerve fibre (NF). The NSs were observed in the ganglia of the myenteric plexus (MP) but not in the submucosal plexus. The mean optical density (MOD) of the whole of PGP9.5-positive nerves and the protein expression level of PGP.5 in the rumen wall both decreased significantly with age. However an obvious increase MOD of PGP.5-positive NFs within the rumen epithelium were observed. In the MP, the nerves and ICs were interwoven to form two complex networks that gradually tightened with age. Furthermore, NSs and nerve trunks were surrounded by a ring-boundary layer consisting of several ICs that became physically closer with aging. Moreover, ICs were located nearby NFs within the ML, forming connections between ICs, smooth muscle cells and axons. This study describes the pattern of neural distribution and its association with ICs in the developing rumen which shed light on the postpartum development of ruminants.

Exploration of the rumen microbial diversity and carbohydrate active enzyme profile of black Bengal goat using metagenomic approach

Black Bengal goats possess a rich source of rumen microbiota that helps them to adapt for the better utilization of plant biomaterial into energy and nutrients, a task largely performed by enzymes encoded by the rumen microbiota. Therefore the study was designed in order to explore the taxonomic profile of rumen microbial communities and potential biomass degradation enzymes present in the rumen of back Bengal goat using Illumina Nextseq-500 platform. A total of 83.18 million high-quality reads were generated and bioinformatics analysis was performed using various tools and subsequently, the predicted ORFs along with the rRNA containing contigs were then uploaded to MG-RAST to analyze taxonomic and functional profiling. The results highlighted that Bacteriodetes (41.38–59.74%) were the most abundant phyla followed by Firmicutes (30.59–39.96%), Proteobacteria (5.07–7.61%), Euryarcheaota (0.71–7.41%), Actinobacteria (2.05–2.75%). Genes that encode glycoside hydrolases (GHs) had the highest number of CAZymes, and accounted for (39.73–37.88%) of all CAZymes in goat rumen. The GT families were the second-most abundant in CAZymes (23.73–23.11%) and followed by Carbohydrate Binding module Domain (17.65–15.61%), Carbohydrate Esterase (12.90–11.95%). This study indicated that goat rumen had complex functional microorganisms produce numerous CAZymes, and that can be further effectively utilised for applied ruminant research and industry based applications.

Telocytes as a Novel Structural Component in the Muscle Layers of the Goat Rumen

Telocytes (TCs) have been identified as a distinct type of interstitial cells, but have not yet been reported in the gastrointestinal tract (GIT) of ruminants. In this study, we used transmission electron microscopy (TEM) and double-labelling immunofluorescence (IF) (antibodies: CD34, vimentin and PGP9.5) to seek TCs and investigate their potential functions in the muscle layers of the goat rumen. TCs were distributed widely in the myenteric plexus (TC-MYs) between the circular and longitudinal muscle layers, within circular muscle layers (TC-CMs) as well as in longitudinal muscle layers (TC-LMs). Ultrastructurally, TCs displayed small cell bodies with several long prolongations—telopodes—harboring alternate thin segments (podomers) and dilated segments (podoms). The podoms contained mitochondria, rough endoplasmic reticulum, and caveolae. Telopodes frequently established close physical interactions with near telopodes, collagen fibers (CFs), nerve fibers (NFs), smooth muscle cells (SMCs), nerve tracts, and smooth muscle bundles, as well as with blood vessels (BVs). Furthermore, both homo- and heterotypic connections were observed. In addition, telopodes were capable of releasing extracellular vesicles (EVs). IF analyses proved that TCs were reliably labeled as CD34+/vimentin+ cells, displaying spindle- or triangle-shaped bodies with long prolongations, consistent with TEM results. Specifically, podoms were visible as obvious bright spots. These positive cells covered entire muscular layers, surrounding ganglions, intermuscular BVs as well as entire smooth muscle bundles, forming a network. TC-MYs were distributed as clusters in the external ganglion, encompassing the entire ganglion and spreading to the muscle layers where TC-CMs and TC-LMs seemingly surround whole smooth muscle bundles. TC-MYs were also scattered within the interior of the ganglion, surrounding each ganglionic neuron, following the glial cells layer. We speculate that TCs support the muscle layer structure of the goat rumen and facilitate intercellular signaling directly or indirectly via the TC network.

Anaerobic biodegradation of cassava wastewater under different temperatures and inoculums

The production of starch generates, as a by-product, the cassava wastewater (manipueira), which can be treated by anaerobic digestion to provide biogas and minimize its polluting potential. The most commonly utilized biomass in the anaerobic digestion is the anaerobic sludge. The literature presents, as an alternative to sludge, bovine manure and ruminal fluids, being scarce the studies with the cassava wastewater. This research evaluated the influence of temperature on the microbial ability of cattle and goat rumen in anaerobically biodegrading the manipueira in substitution to the anaerobic sludge. The cattle and goat rumen specific methanogenic activities (SMA) were compared with that of the anaerobic sludge. Subsequently, by using the inoculum which had the best SMA results, cassava wastewater biodegradability tests were performed, investigating the kinetics of the organic matter removal and methane production at 32 ° C and 39 ° C. The bovine rumen presented better results in the SMA (0,315 g COD-CH4 g VSS.d-1) and methane production (1,026 mL). The temperature of 32 °C did not influence the activity of bovine ruminal inoculum as the kinetics of the biodegradation of the manipueira did not differ for the evaluated temperatures (0.1799 d-1 at 32°C and 0.1781 d-1 at 39°C). Bovine rumen achieved glucose reduction of 76% and 80% and methane yield of 77% and 79% for the tests at 32°C and 39°C, respectively. It is inferred that this type of inoculum might be used in reactors of anaerobic digestion processes for the treatment of the cassava wastewater at the ambient temperature of the semiarid region.

Temperature and humidity index (THI)-induced rumen bacterial community changes in goats.

The rumen microbiome is thought to play an important role in maintaining normal gastrointestinal metabolism and nutrient absorption in ruminants. The present study was designed to investigate the effect of heat stress on the rumen microbiome of goats using 16S rRNA sequencing technology. Six female goats were randomly allocated into two control metabolic chambers: A and B (in which the temperature and humidity could be precisely controlled with a precision deviation of ± 0.5°C and ± 5%, with three goats/chamber). Dynamic changes in the rumen bacterial community were detected under 16 gradually increasing temperature and humidity indexes (THIs). Heat stress had no significant effect on alpha diversity but affected the main phyla and genera of the goat rumen microbiota. With a deeper level of heat stress, the TH groups formed a distinct cluster that differed from that of the control check (CK) group. The dominant phylum transitioned from Firmicutes to Bacteroidetes, and co-exclusion occurred between these two phyla. With the increase in THI, the content of probiotics in the Lachnospiraceae_ND3007_group (P < 0.05) decreased, and the abundance of pathogenic bacteria, such as Erysipelotrichaceae_UCG-004 and Treponema_2, increased; however, the difference between the groups was not significant (P > 0.05). Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict bacterial function, and we found that the ambient environment significantly affected the balance between carbohydrate and energy metabolism (P < 0.05). In conclusion, heat stress changed the composition of rumen microbes and affected metabolic function. This experiment provides a theoretical basis for exploring the effects of environmental factors on the rumen of goats.

Anthelmintic efficacy of ethanol extract of Nigella Sativa on lactate dehdrogenase of Cotylophoron Cotylophorum

Paramphistomosis is a major disease caused by stomach flukes. The Paramphistome Cotylophoron Cotylophorum lives in rumen and reticulum of sheep, goats and other domestic ruminants. The present study was undertaken to elucidate the anthelmintic effect of Ethanol extract of Nigella sativa on the lactate dehydrogenase (LDH) of C. Cotylophorum in vitro. The parasites were maintained in vitro in Hedon-Fleig solution (pH 7.0) which is the best medium for their survival. Parasites were exposed to five different sub-lethal concentration of ethanol extract of Nigella sativa (NsEE) for 2, 4 and 8h. LDH and Protein content in the sample was estimated following standard procedures. The inhibition of LDH catalyzing oxidation of lactate results in the reduced production of malate, which subsequently results in the reduced production of ATP. Decreased production of ATP results in the death of parasites. The present investigation amply demonstrated that N. sativa could be used as a potential phytotherapeutic drug to combat paramphistome infection in livestock.

Aquatic weed Ipomoea aquatica as feed ingredient for rearing Rohu, Labeo rohita (Hamilton)

Environment friendly management of the aquatic weed Ipomoea aquatica is possible through its utilization as ingredient in fish feed. In this study leaf meal of I. aquatica was fermented by phytase producing bacteria Stenotrophomonas maltophilia KUAKSP1 (GenBank Accession No. KY790423) isolated from the rumen of goat. Four experimental feed were formulated by replacing 0% (T1, Control), 25% (T2), 50% (T3) and 75% (T4) of fishmeal (FM) with the fermented Ipomoea leaf meal (FILM) as source of protein. Fingerlings of rohu, Labeo rohita were reared on these feed for eight weeks. The results showed that growth, daily retention of protein and nutrient deposition in L. rohita were significantly higher in FILM supplemented feed as compared to control. Best growth was obtained in 25% replacement of FM by the FILM supplemented feed (T2). α-amylase activity in T2 was significantly higher than control and other treatments (T3 and T4), while protease activity significantly increased in T3 and T4 as compared to T1 and T2. It was concluded that L. rohita could utilize limited amount of carbohydrate in the feed, there by sparing protein to render better growth of the fish.

Immunohistochemical Study on the Ruminal Wall of Adult Baladi Goats (Capra hircus)

A total number of eight Baladi goats (Capra hircus)of both sexes aged between 11-18 months-old were used to describe the histological structure of the ruminal wallin addition to investigate the immunohistochemical localization and distribution of ki-67, caspase 3 and vimentin. The results revealed no sex differences in the ruminal wall histology or immunohistochemistry.Histologically, the ruminal wall consisted of mucosa-submucosa, muscularis and serosa. The mucosaof the rumen was thrown into ruminal papillae that were coveredby keratinized epithelium. The submucosa was consisted of loose connective tissue lacking glands. Circular and longitudinal smooth muscle layers were the components ofmuscularis however, the rumen was surrounded externally by typical serosa. The immunohistochemical study was done using theavidin-biotin immunoperoxidase method. Ki-67 immunostaining was limited to nuclei of basal cells layer in the ruminal epitheliumwhich explained the importanceof ki-67 in epithelial cells proliferation as well askeratin biosynthesis. Caspase 3 immunostaining was localized in cytoplasm and nuclei of some epithelial cells that were going to apoptosis.The vimentinimmunostaining was widely spread in epithelial cells as well as,fibroblasts in propria and submucosa, and endothelia of blood vessels.This showed theimportance of vimentin asan intermediate filament protein. Detection of vimentin in the glial cells of enteric plexuses indicatedits supportive role in the nervous control of the rumen. Overall, the histological and immunohistochemical findings of this study explainedthe structure-function relationship of the rumen of Baladi goats

Nitrogen degradation of the limestone-urea mixtures in the rumen of goats

This experiment was aimed to study the degradation kinetics of limestone-urea mixtures in the goats rumen using the nylon bag technique. Samples of limestone were obtained from two limestone mountains, Pamotan Subdistrict of Central Java Province and Wonosari Subdistrict of Yogyakarta Province. The mixtures were created by combining urea at levels 25, 50, 75and 100%; respectively with two limestones on the basis of their Ca contents: L0U100, LP25U75, LP50U50; LP75U25, LW25U75; LW50U50; and LW75U25. The soluble fraction, potentially degradable fraction, the degradation rate of potentially degradable fraction, and effective degradation of respective dry matter (DM) and nitrogen (N) ruminal degradation kinetics were measured in each mixture. The mixture of LP75U25 had lowest effective and degradation rate of potentially degradable fraction (P&lt;0.05) respectively for DM and N compared with those of other mixtures. In conclusion, the limestone-urea mixture of LP75U25 could be suggested as a dietary supplement of ruminal N slow release.

Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library

This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens. A novel gene, KG42, was isolated from one of the 17 xylan-degrading metagenomic fosmid clones obtained from black goat rumens. The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a “salad-bowl” shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues. KG42 xylanase protein has at best 40% sequence identity with other homologous GH10 xylanase proteins. The enzyme displayed its optimum activity at pH 5.0 and 50 °C. The enzyme was thermally stable at pH and temperature ranges of 5.0–10.0 and 20–60 °C, respectively. Substrate specificity and hydrolytic patterns implied that the KG42 xylanase functions as an endo-β-1,4-xylanase (EC 3.2.1.8). The KG42 xylanase was also used for the preparation of bifidogenic xylan hydrolysates, demonstrating its potential applications toward preparing prebiotic xylooligosaccharides.

Metagenomic Mining and Functional Characterization of a Novel KG51 Bifunctional Cellulase/Hemicellulase from Black Goat Rumen.

A novel KG51 gene was isolated from a metagenomic library of Korean black goat rumen and its recombinant protein was characterized as a bifunctional enzyme (cellulase/hemicellulase). In silico sequence and domain analyses revealed that the KG51 gene encodes a novel carbohydrate-active enzyme that possesses a salad-bowl-like shaped glycosyl hydrolase family 5 (GH5) catalytic domain but, at best, 41% sequence identity with other homologous GH5 proteins. Enzymatic profiles (optimum pH values and temperatures, as well as pH and thermal stabilities) of the recombinant KG51 bifunctional enzyme were also determined. On the basis of the substrate specificity data, the KG51 enzyme exhibited relatively strong cellulase (endo-β-1,4-glucanase [EC 3.2.1.4]) and hemicellulase (mannan endo-β-1,4-mannosidase [EC 3.2.1.78] and endo-β-1,4-xylanase [EC 3.2.1.8]) activities, but no exo-β-1,4-glucanase (EC 3.2.1.74), exo-β-1,4-glucan cellobiohydrolase (EC 3.2.1.91), and exo-1,4-β-xylosidase (EC 3.2.1.37) activities. Finally, the potential industrial applicability of the KG51 enzyme was tested in the preparation of prebiotic konjac glucomannan hydrolysates.

TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT

This study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39° C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species.

Bioaugmentation of anaerobic digesters treating lignocellulosic feedstock by enriched microbial consortia.

Three different bioaugmentation cultures enriched from natural and engineered cellulolytic environments (cow and goat rumen, a biogas reactor digesting sorghum biomass) were compared for their enhancement potential on the anaerobic digestion of wheat straw. Methane yields were determined in batch tests using the Automatic Methane Potential Test System operated for 30 days under mesophilic conditions. All cultures had positive effects on substrate degradation, and higher methane yields were observed in the bioaugmented reactors compared to control reactors set up with standard inoculum. However, the level of enhancement differed according to the type of the enrichment culture. Methane yield in batch reactors augmented with 2% cow rumen derived enrichment culture was increased by only 6%. In contrast, reactors amended with 2% goat rumen derived enrichment culture or with the bioaugmentation culture obtained from the biogas reactor digesting sorghum biomass produced 27 and 20% more methane, respectively. The highest methane yield was recorded in reactors amended with 6% goat rumen derived enrichment culture, which represented an increase by 36%. The microbial communities were quite similar at the end of the batch tests independently of the bioaugmentation sources, indicating that the introduced microbial communities of the enrichment cultures did not dominate the reactors.

Investigation of methods for extraction of bacterial DNA metagenome from goat rumen

Microorganisms, particularly bacteria, in the ruminant's rumen are valuable genetic resources that many scientists interested in. In recent years, the application of next-generation sequencing technologies allows direct decoding an extracted DNA metagenome in each ecological community without culture, increasing the efficiency of exploiting interested genes. Notably, the quantity and quality of extracted DNA play an important role in getting a reliable metagenome database. In this study, DNA metagenome from goat rumen fluid was extracted by five different methods RBB (repeated bead beating plus column), RBBC (repeated bead beating), PSP1, PSP2 (PSP®Spin Stool DNA Kit, protocol 1, 2, Germany) và QIA (QIAamp® DNA Stool Mini Kit, Germany). The results showed that DNA metagenome obtained by all methods had A260/280 greater than 1.8. DNA extracted by the RBB method had high DNA concentration but low A260/230 values (less than 1.4) and still contained Taq polymerase inhibitor. After purifying by QIA column, A260/230 values of RBB-extracted DNA significantly increased up to 2.0 and Taq polymerase inhibitor in samples were removed. However, the concentrations decreased by 57% that nearly equivalent to concentration of DNA metagenome obtained by QIA. The method using PSP®Spin Stool DNA kit produced the highest DNA concentrations (from 149.7 to 195.5 ng/µl) with A260/280 ratios of 1.9 and A260/230 ratios of 1.8 to 1.9. Morever, this method was able to remove polymerase inhibitor and be performed on short time. Therefore, the PSP®Spin Stool DNA kit is a suitable method for DNA metagenome extraction of bacteria from goat rumen. DNA obtained by this method fulfilled all criteria about quality and concentration for sequencing by next-generation sequencing Illumina.

Profile of Rumen Fermentation and Blood Urea Nitrogen Concentration of Kacang Goat Fed Total Mixed Ration Vs. Roughage

Kacang goat is usually reared traditionally fed natural grass and having inferior performance. Many researches had been done to improve the performance. Total mixed ration (TMR) containing soybean meal (SBM) and fish meal (FM) could increase the performance of Kacang goat, but the profile of rumen fermentation has not been published. Therefore, this study investigated the profile of rumen fermentation and blood urea Nitrogen concentration (BUN) in Kacang goat fed natural grass (roughage) and TMR. Twelve yearling Kacang buck, 15.2-19.6 kg were arranged in completely randomized design. The treatments were NG (natural grass), FM (TMR containing FM), and SBM (TMR containing SBM). The TMR were formulated containing 15% crude protein and 56-58% TDN. Data were analyzed by one way ANOVA. Rumen pH 6 hours after feeding of NG (7.4) was higher (P<0.01) than that of FM (6.2) and SBM (6.4). This lowering pH of TMR was caused by increasing volatile fatty acids (VFA). The VFA total of FM (129.7 mmol/l) and SBM (153.1 mmol/l) were higher than that of NG (86.4 mmol/l). At 3 and 6 hours after feeding, ammonia in the rumen of SBM was higher than that of NG and FM, indicating higher degraded protein. The BUN at 3 hours after feeding of SBM was higher than that of NG. It can be concluded that protein in SBM was degraded higher than others and the lower pH in rumen of TMR goats was caused by higher VFA produced by TMR goats compared to NG goats.