A microdialysis technique was used in male rats to directly assess the postulate that pubertal maturation is associated with accelerated GnRH pulsatility. Juvenile male rats, postnatal d 43 or 45 (n = 4) were stereotaxically fitted with guide cannulas directed toward the lateral median eminence, and repeated microdialysis experiments were conducted over 4-6 d. In each session, samples were collected continuously over 12 h (0900-2100 h) at 5-min intervals Results from individual peripubertal animals were pooled into two time bins for postnatal d 45-47 and 48-50, respectively, and GnRH characteristics were compared between the two epochs. The GnRH pulse frequency and mean GnRH concentration were significantly elevated at 48-50 d compared with 45-47 d. The GnRH pulsatility characteristics for 45-47 d vs. 48-50 d were as follows: pulse frequency, 0.74 +/- 0.16 vs. 1.79 +/- 0.19 pulses/h (P < 0.05); pulse amplitude, 254.1 +/- 22.3 vs. 347.2 +/- 15.8 deltapg/ml (difference in value from trough to peak); and mean release, 0.55 +/- 0.03 vs. 2.04 +/- 0.04 pg/5 min (P < 0.05). An additional two rats were dialyzed only once on postnatal d 50 to assess the effects of repeated sampling; the GnRH pulse characteristics in these animals were similar to those in rats sampled for a third or fourth time on postnatal d 48-50. To further assess the possible effects of repeated sampling on GnRH release profiles, a group of adult male rats (postnatal d 95-105; n = 3) was also dialyzed on four consecutive days. In these rats no significant alteration in GnRH pulse generator activity was observed over the four sessions. Moreover, the increase in GnRH pulse frequency observed in the peripubertal rats was found to be sustained in adult animals. To better understand the temporal relationship of GnRH pulse generator activity to reproductive maturation, groups of male rats were killed from postnatal d 45-56 along with an adult group at 95-105 d (n = 5/group) and examined for physiological signs of reproductive development. Gradual increases in serum levels of LH and testosterone and decreases in FSH and inhibin B were seen from postnatal d 45-56 to adulthood. Mature spermatozoa were found in the vas deferens by postnatal d 53. Our results demonstrate that in the late juvenile stage of male rat development, GnRH pulse generator activity is gradually accelerated over the course of consecutive days. This acceleration occurs over a period during which serum LH and testosterone are rising to adult levels, and it precedes the presence of mature spermatozoa in the vas deferens by 3 d. Our observations provide direct support for the hypothesis that an acceleration of GnRH pulsatility is the critical neural stimulus for the initiation of pubertal maturation in males. The peripheral and central cues that prompt the pubertal activation of the GnRH pulse generator remain to be characterized.