AbstractCubeSats have become versatile platforms for various space missions (e.g., on-orbit servicing and debris removal) owing to their low cost and flexibility. Many space tasks involve proximity operations that require precise guidance, navigation, and control (GNC) algorithms. Vision-based navigation is attracting interest for such operations. However, extreme lighting conditions in space challenge optical techniques. The on-ground validation of such navigation systems for orbital GNC becomes crucial to ensure their reliability during space operations. These systems undergo rigorous testing within their anticipated operational parameters, including the exploration of potential edge cases. The ability of GNC algorithms to function effectively under extreme space conditions that exceed anticipated scenarios is crucial, particularly in space missions where the scope of errors is negligible. This paper presents the ground validation of a GNC algorithm designed for autonomous satellite rendezvous by leveraging hardware-in-the-loop experiments. This study focuses on two key areas. First, the rationale underlying the augmentation of the robot workspace (six-degree-of-freedom UR10e robot + linear rail) is investigated to emulate relatively longer trajectories with complete position and orientation states. Second, the control algorithm is assessed in response to uncertain pose observations from a vision-based navigation system. The results indicate increased control costs with uncertain navigation and exemplify the importance of on-ground testing for system validation before launch, particularly in extreme cases that are typically difficult to assess using software-based testing.