The present study was aimed to investigate the genotoxic and apoptotic effects of glyphosate (GLP) in Roundup formulation along with mitigation of two potent antioxidants that is, vitamin C and E in caprine granulosa cells in vitro. The entire work was done in a dose and time dependent manner where different concentrations of GLP (0.1, 2.0, and 4.0mg/ml) in Roundup and antioxidants (0.5 and 1.0 mM) were employed to culture of granulosa cells for exposure durations of 24, 48, and 72 h. Analysis of GLP-induced geno-toxicity was accomplished by using single cell gel electrophoresis (comet assay) assay. Results have shown increased incidences of DNA fragmentation, evidenced by presence of different types of comets (Type 1-Type 4) in Roundup-GLP- exposed groups in contrast to the control group (Type 0 comet). However, mitigation by both vitamin C and E was significant (p < .05) in combating the GLP-induced genotoxicity in granulosa cells in a concentration- and time-dependent manner. The results of our study provide a clear indication of the ameliorative actions of vitamin C and E against Roundup-GLP-induced genotoxicity that instigate apoptosis in ovarian granulosa cells of caprine.