Glycosmis Pentaphylla Research Articles

Antiarthritic activity of a polyherbal formulation against Freund's complete adjuvant induced arthritis in Female Wistar rats.

Objectives:To formulate a polyherbal formulation and evaluate its antiarthritic activity against Freund's complete adjuvant induced arthritis in Female Wistar rats.Materials and Methods:Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including arthritis. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Arthritis was induced in female Wistar rats using Freund's complete adjuvant (FCA), and the antiarthritic effect of polyherbal formulation was studied at doses of 250 and 500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, blood samples were collected for biochemical and hematological analysis. The radiological examination was carried out before terminating the study.Results:Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of indomethacin. The antiarthritic activity of polyherbal formulation is supported by biochemical and hematological analysis.Conclusion:The polyherbal formulation showed signinicant antiarthritic activity against FCA-induced arthritis in female Wistar rats.

Biodiversity and Landscape Use of Sea Buckthorn (Hippophae rhamnoides L.) in the Coruh Valley of Turkey

The Sea buckthorn, one of the most important wild edible fruits widely grown in Northeastern part of Turkey, is characterized by resistance to hard environmental conditions and looks spectacular, especially in autumn and winter when it is decorated with orange berries. Climatic conditions in Northern Anatolia are suitable for the growing of the Sea buckthorn, which can be encountered in various dry areas as well as by riversides. Local people traditionally processed or preserved sea buckthorn fruits (berries). The decorative sea buckthorn shrubs/trees and berries are an important element of natural landscape in Northeastern Anatolia. The aim of the study was to define the biodiversity among trees/shrubs, berries and leaves of the Sea buckthorn accessions based on morphological and biochemical data. Results showed a high diversity among accessions in terms of plant, leaf and berry characteristics. Berry diameter, length and 100 berry weight ranged from 5.48 to 7.18 mm; 6.64 to 9.14 mm and 15 to 26 g, respectively. A wide variability of berry skin colour was observed to be yellow, light yellow, dark yellow, yellow orange, orange and dark orange. The anthocyanin content varied from 7 to 38 mg/l berry juice.

Phenolic glycosides from Glycosmis pentaphylla

Three new phenolic glycosides, named as glycopentosides A–C (1–3), along with nine known compounds were isolated from the n-BuOH extract of stems of Glycosmis pentaphylla. Their structures were determined by using spectroscopic and chemical methods. Bioassay showed that compound 10 (tachioside) could inhibit nitric oxide production in lipopolysaccharides-stimulated RAW 264.7 cells with IC50 value of 12.14 μM.

Freeze Response of Citrus and Citrus-related Genotypes in a Florida Field Planting

A test population consisting of progenies of 92 seed-source genotypes (hereafter called “parent genotypes”) of Citrus and Citrus relatives in the field in east–central Florida was assessed after natural freeze events in the winters of 2010 and 2011. Eight seedlings per parent genotype were planted in a randomized complete block design; however, as a result of mortality, the number of plants assessed in some genotype groups was reduced at some or all sampling dates. The citrus diseases huanglongbing and citrus canker were endemic in the planting and may have influenced tree response to cold temperatures. Unusually low temperatures (near –4 °C each winter) for east–central Florida were experienced during the trial period. Defoliation and dieback were significantly greater in the winter of 2011 than in the winter of 2010. The winter in 2011 was preceded by a period of extraordinarily low temperatures in mid-December with no period of cool temperatures to allow trees to acclimate. In 2010 the average defoliation was 53% ± 28% and less than 13% of the trees exhibited any noticeable dieback, whereas in 2011, the average defoliation and dieback were 93% ± 17% and 51% ± 35%, respectively. Within the genus Citrus, several progenies were identified that had 16% to 24% dieback in 2011 and these were from parent genotypes C. reticulata (CRC 2590) (23%), C. sinensis (CRC 3858) (24%), C. maxima (CRC 3945) (16%), C. hassaku (CRC 3907 and 3942) (16% and 17%), C. aurantium (CRC 628 and 2717) (18% and 7%), C. taiwanica (CRC 2588) (21%), and C. neo-aurantium (C. obovoidea + C. unshiu graft chimera) (CRC 3816) (23%). Within other genera in the Aurantiodeae, Poncirus trifoliata (CRCs 301, 3957, 3549, and 4007), Severinia buxifolia (CRC 1497), Bergera koenigii (CRC 3165), and Glycosmis pentaphylla (CRC 3285) had the least amount of dieback, all at less than 23%. The two species within the Toddalioideae subfamily of the Rutaceae (Casimiroa edulis and Zanthoxylum ailanthoides) had among the least amount of dieback (1% and 8%, respectively). When considered by groups, the Citrons and Australian natives had the greatest amount of dieback in 2011, 68% and 65%, respectively. The trifoliates (Poncirus and hybrids) had the least dieback, ranging from 4% to 40%. The information from this study may be useful in germplasm enhancement and Citrus breeding targeting greater cold tolerance.

Glycoborinine induces apoptosis through mitochondrial pathway in HepG2 cells

Glycoborinine (GB), a natural carbazole alkaloid isolated from Glycosmis pentaphylla, has been shown to be a potential molecule against cancer cells. In this study, the cell-signaling pathway of its anti-tumor activity was investigated. MTT assay result showed that GB inhibited HepG2 cell proliferation in a dose- and time-dependent manner and 50% inhibiting concentration (IC50) of GB-induced cell death was 39.7 μM for a period of 48 h. GB-induced HepG2 apoptosis was confirmed by Hochest 33258 staining and PI staining. The level of reactive oxygen species (ROS) was measured with H2DCF-DA staining and the change of mitochondrial membrane potential (△Ψm) was analyzed with tetrechloro-tetraethylbenzimidazolcarbocyanine iodide (JC-1) probe. Results showed that GB at 12.5, 25, and 50 μM promoted ROS production. GB induced HepG2 apoptosis through a mitochondrial apoptotic pathway, which was demonstrated by GB-induced increase in the ratio of Bax/Bcl-2, cytochrome C release, the ratio of cleaved caspase-3/procaspase-3, and the ratio of cleaved poly ADP-ribose polymerase (cleaved PARP)/poly ADP-ribose polymerase (PARP). To summarize, this study demonstrated that GB could induce HepG2 apoptosis through the mitochondrial-dependent pathway, which might provide a promising approach to cure liver cancer with GB.

Antidiabetic Activity of Polyherbal Formulation in Streptozotocin – Nicotinamide Induced Diabetic Wistar Rats

Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including diabetes mellitus. The antidiabetic activity of the individual plant parts is well known, but the synergistic or combined effects are unclear. The concept of polyherbalism has been highlighted in Sharangdhar Samhita, an Ayurvedic literature dating back to 1300 AD. Polyherbal formulations enhance the therapeutic action and reduce the concentrations of single herbs, thereby reducing adverse events. The aim of the present study is to formulate a polyherbal formulation and evaluate its antidiabetic potential in animals. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. The quality testing parameters of the polyherbal formulation were within the limits. Fingerprint analysis of the polyherbal formulation showed effective separation at 366 nm, and it revealed that the active compound present in the polyherbal formulation and the active compounds present in all the three extracts were the same. The acute toxicity studies of the polyherbal formulation did not show any toxic symptoms in doses up to 2000 mg/kg over 14 days. The oral antidiabetic activity of the polyherbal formulation (250 and 500 mg/kg) was screened against streptozotocin (50 mg/kg; i.p.) + nicotinamide (120 mg/kg; i.p.) induced diabetes mellitus in rats. The investigational drug was administered for 21 consecutive days, and the effect of the polyherbal formulation on blood glucose levels was studied at regular intervals. At the end of the study, the blood samples were collected from all the animals for biochemical estimation, and the animals were sacrificed and the liver and pancreatic tissues were collected for histopathologic analysis. Polyherbal formulation showed significant antidiabetic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of glibenclamide. The antidiabetic activity of polyherbal formulation is supported by biochemical and histopathologic analysis.

Sea buckthorn (Hippophae rhamnoides)- an important fodder for bactrian camel in Ladakh region

Hippophae rhamnoides, the sea-buckthorn or leh berry is a hardy, deciduous shrub with yellow or orange berries in the family Elaeagnaceae. It grows naturally in Ladakh region of Jammu and Kashmir and locally known as a Tsermang or tasru- wonder plant. It is mostly browsed by the bactrian camel in the Nubra valley of Ladakh. The nutritional, biochemical and anti-oxidant attributes of sea-buckthorn have been discussed.

Carbon Stock Assessment in the Vegetation of the Chitteri Reserve Forest of the Eastern Ghats in India Based on Non-Destructive Method Using Tree Inventory Data

Carbon stock assessment in the vegetation of forest ecosystems has attracted worldwide attention following their role in the mitigation of greenhouse gas and climate change. The aim of the present study was to assess the total carbon stock of trees in the Chitteri reserve forest of the Eastern Ghats in India, based on tree inventory. The entire stretch of the forest covering 742 km2 area was divided into twenty grids of 6.25 km × 6.25 km, and from each grid a transect of size 5 m × 1000 m was established. A total of 5022 live trees (≥ 9.5 cm diameter at breast height) representing 143 species were inventoried from the twenty transects (totaling 10 ha). Carbon stock of each tree was determined by a non-destructive method following standard allometric equation using the tree diameter. The total carbon stock of the tree vegetation for the 10 ha sampled in the Chitteri reserve forest was 585.50 tonnes. The average carbon stock for the twenty 0.5 ha transect was 29.28±21.7 tonne. The minimum and maximum carbon stock estimated for the 20 transects was 1.98 t/0.5 ha and 76.89 t/0.5 ha, respectively. The average carbon stock sequestered by a single tree was 0.12±0.24 t/tree. The maximum carbon value was observed for Mangifera indica (1.73 t/tree) and the minimum was for Glycosmis pentaphylla (0.01 t/tree). However, overall contribution to the total carbon stock of tree vegetation in the Chitteri reserve forest was observed greater for Memecylon edule (8.5 %), followed by Canthium dicoccum var. dicoccum (7.4 %), Anogeissus latifolia (6.4 %), Albizia amara (4.9 %) and Mangifera indica (4.4 %). A significant strong positive relationship was observed between carbon stock with tree density (R2=0.689), but not with tree diversity (R2=0.422) and with altitude (R2=0.399) of forest location.

Antidiabetic and Analgesic Effects of Glycosmis pentaphylla (Retz.) in Swiss Albino Mice

Background and purposes: Glycosmis pentaphylla (Retz.) Correa, a medicinal plant is popularly used as herbal remedy for various ailments in Bangladesh. It was also reported that GP has both anti-hyperglycemic and analgesic effects and being widely used to reduce blood glucose and to alleviate pain for many years in this region though published literatures are scarce. The present study was designed to evaluate whether ethanolic extract of Glycosmis pentaphylla (GP) have antihyperglycemic and analgesic effects. A total of 60 Swiss Albino male mice of nine weeks (weight, 20-25g) were used for investigation. Of them, 40 were made diabetic by alloxan. They were investigated in two groups – a) 20 mice by oral glucose tolerance test (4 samples OGTT) – at 0, 30, 90 and 120 min; and b) 20 mice for a week-long antihyperglycemic activity on day 0, 1, 3 & 7. Both the groups were subdivided into four, each having 5 mice – i) the ‘control’ received only 0.5% methyl cellulose as vehicle; ii) ‘Standard’ received vehicle plus metformin; iii & iv) test ‘DGP250’ & ‘DGP500’ received vehicle plus GP extract with 250 & 500 mg /kg, respectively. For the analgesic activity, 20 mice were investigated in four subgroups, each having 5 mice and similar steps were adopted. Here, vehicle was used 1% Tween 80 and intra-peritoneal injection of Acetic acid for eliciting pain in all four subgroups. The ‘standard’ group got diclofenac sodium for comparison with the test groups ‘GP250’ and ‘GP500’. In OGTT, Ethanolic extract of GP250 and GP500 reduced blood glucose at 90 min. But the levels of reduction were more significant at 120 min, 50.7% by GP250 and 66% by GP500 (p<0.001). The reduction is almost comparable with that induced by metformin. Likewise, for a weeklong anti-hyperglycemic activity, the GP extracts were found as equally effective as metfomin, which was also dose dependent. In addition to antihyperglycemic effect, the ethanolic extract of GP showed significant analgesic effect that was also dose dependent. Our results indicate that GP extract has antihyperglycemic effect in both short and in weeklong duration, which is almost comparable to Metformin HCL, a known and widely used antihyperglycemic agent. The GP extract was also found to have an analgesic effect almost comparable to diclofenac sodium, a known analgesic drug. Further study is needed to confirm the anti-hyperglycemic and analgesic effect of GP including its side effects in long term use. DOI: http://dx.doi.org/10.3329/imcj.v6i1.14721 Ibrahim Med. Coll. J. 2012; 6(1): 21-26

Alkaloids from the Stems of Glycosmis pentaphylla

AbstractA new simple carbazole alkaloid, 4‐(7‐hydroxy‐3‐methoxy‐6‐methyl‐9H‐carbazol‐4‐yl)but‐3‐en‐2‐one (1), and two new dimeric carbazole alkaloids, bisglybomine B (2) and biscarbalexine A (3), together with seven known alkaloids, were isolated from the stems of Glycosmis pentaphylla. Their structures were elucidated by spectroscopic methods, especially 2D‐NMR techniques.

Two new flavanols from Glycosmis pentaphylla

Two new flavanols, glycoflavanones A (1) and B (2), which were found to possess α-pyrone moiety, together with five known compounds 4′-O-methylgallocatechine (3), β-sitosterol (4), alphitol (5), 3,4-dimethoxy-5-hydroxy-trans-cinnamyl alcohol (6), and oxyresveratrol (7), were isolated from the stems of Glycosmis pentaphylla by normal-phase and reverse-phase silica gel column chromatography. Their structures were determined on the basis of chemical and spectroscopic analyses.

Antinociceptive activity of the methanol extracts of leaves of Eugenia fruticosa (Roxb.) and Glycosmis pentaphylla (Retz.) in Swiss albino mice

The present study was designed to evaluate analgesic potential of the methanolic extracts of the leaves of the both Glycosmis pentaphylla (Rutaceae) and Eugenia fruticosa (Myrtaceae). The analgesic activity was evaluated using the acetic acid (0.7% i.p.)-induced writhing inhibition method in swiss albino mice. The methanolic extract of leaves of Glycosmis pentaphylla, at the dose of 200 and 400 mg/kg body weight significantly (p<0.001) reduced the number of writhes with 31.97% and 44.21% of inhibition, respectively compared to control group. The methanolic extracts of leaves of Eugenia fruticosa significantly and dose dependently reduced the pain threshold (p<0.001) with 51.02 and 72.1% of writhing inhibition when compared to the control group at the dose of 200 and 400 mg/kg body weight, respectively which were comparable to that of the standard drug Diclofenac Na (65.31% inhibition of writhing, p<0.001). The results of the study demonstrated the potential antinociceptive activity of the methanolic extracts of the leaves of Glycosmis pentaphylla and Eugenia fruticosa which validated the traditional uses of the both plants in painful diseases and further investigations to elucidate the mechanism of action are required.

Glycopentaphyllone: The first isolation of hydroperoxyquinolone from the fruits of Glycosmis pentaphylla

A new hydroperoxyquinolone alkaloid, glycopentaphyllone (1), along with nine compounds (2–10), was isolated from the fruits of Glycosmis pentaphylla. Their structures were elucidated on the basis of spectroscopic methods. The absolute configuration of glycopentaphyllone at C-2′ was established as S-configuration by applying Mosher's method. In addition, the completed assignments of 13C NMR as well as 2D NMR spectral data of compound 5 were reported herein for the first time. Also, all isolates were evaluated for antibacterial activity against Escherichia coli TISTR 780, Salmonella typhimurium TISTR 292, Staphylococcus aureus TISTR 1466, and Methicillin-resistant S. aureus SK1.

The apoptosis inducing effect of Glycosmis pentaphylla (Retz.) Correa and its influence on gene expression in hepatocellular carcinoma cell line, Hep3 B

Ethnopharmacological relevanceGlycosmis pentaphylla (Retz.) Correa is used in Indian traditional medicine against jaundice and other liver disorders. Aim of the studyThe study aims to determine the in vitro anticancer and apoptosis inducing activity of Glycosmis pentaphylla in hepatocellular carcinoma cell line, Hep3 B. Materials and methodsThe cytotoxic and apoptosis inducing activity of the crude extract and active fractions were estimated on Hep3 B and RAW264.7 cell lines by MTT assay, Hoechst staining, DNA fragmentation, morphological studies, reverse transcription polymerase chain reaction and anti-poly-(ADP-ribose)-polymerase assays. The phytochemical profiling of active extract was done by TLC and HPTLC methods. ResultsEthanol extract of Glycosmis pentaphylla was more effective than other extracts in reducing the proliferation of Hep3 B cells. As revealed by the results from DNA fragmentation, Hoechst staining, morphological studies, RT-PCR, PARP cleavage and gene expression studies, active extract induced apoptosis on Hep3 B cell line in concentration and time dependent manner with increase in the Bax/Bcl2 gene expression ratio. Chemo profiling data revealed the presence of flavonoid in the active fraction. ConclusionsThe study showed that major active component in the ethanol extract of Glycosmis pentaphylla is a flavonoid which induces apoptosis on cancer cell line, Hep3 B, by increasing the expression ratio of Bax/Bcl2 genes in a time and dose dependent manner.

Abundance of Citrus Leafminer Larvae on Citrus and Citrus-related Germplasm

The citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), is a key pest in most citrus-growing regions worldwide. Adult citrus leafminers oviposit primarily on young elongating flush of Citrus as well as other Rutaceae and some ornamental plants. Larvae feed on the epidermal cell layer of developing leaves and injury to leaves provides a pathway for infection by the bacterium Xanthomonas citri subsp. citri (Hasse), the causal agent of Asiatic citrus canker. In this study, we quantified abundance of citrus leafminer larvae on progeny of 87 seed parent genotypes of Citrus and Citrus relatives (family Rutaceae) in the field in East–central Florida to identify those that have low abundance of leafminers. Progeny from the 87 parent genotypes varied in abundance of the leafminer. Progeny of 15 parent genotypes had a high mean abundance of more than six leafminers per flush shoot. All but one of these genotypes were in the Citrus genus. Progeny of 16 parent genotypes had zero, or nearly zero, leafminers, but none were from the Citrus genus. However, many of these 16 genotypes were from genera closely related to true citrus (subtribe Citrinae) and are sexually compatible with Citrus. Progeny of two parent genotypes in the subfamily Toddalioideae and Glycosmis pentaphylla (Retz.) Corr. also had a low abundance of leafminer. Glycosmis pentaphylla also is a poor host for the Asian citrus psyllid, Diaphorina citri Kuwayama, and has biochemical resistance to the citrus weevil, Diaprepes abbreviatus (L.), so this genotype as well as others identified as poor hosts for the leafminer may prove useful in breeding programs aimed at reducing the abundance of multiple insect pests on citrus.

Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) organs

Hippophaë rhamnoides L. (Elaeagnaceae), commonly known as sea buckthorn, is a thorny bush with orange berries naturally distributed in Asia and Europe. Fruits of H. rhamnoides have been used by Chinese, Mongolian and Tibetan medicines for decades, and possessed considerable medicinal and nutritional values like antimicrobial, antitumoral, antioxidant and dermatological effects [1,2].

Biotechnological interventions in sea buckthorn (Hippophae L.): current status and future prospects

Sea buckthorn (Hippophae L., Elaeagnaceae) is an economically and ecologically important medicinal plant comprising of species which are winter hardy, dioecious, wind-pollinated multipurpose shrubs bearing yellow or orange berries with nitrogen-fixing ability. It grows widely in cold regions of Indian Himalayas, China, Russia, Europe and many other countries. It is commonly known as ‘cold desert gold’ due to its high potential as a bio-resource for land reclamation, reducing soil erosion and its multifarious uses. The wild populations are being used for harvesting economic benefits with negligible plantation efforts. Although this plant has many excellent traits, it is still in an early phase of domestication. This woody plant is prone to many pests and diseases which destroy the plants and halt its commercial production. Limited progress has been made for improvement of sea buckthorn through breeding programs due to long juvenile period and lack of QTL linkage map, which makes screening of mapping populations a time-consuming and labor-intensive task. Conventional propagation methods, i.e. seeds, softwood and hardwood cuttings, and suckers are in place but are cumbersome and season dependent. Therefore, application of modern tools of biotechnology needs to be standardized for harnessing maximum benefits from this nutraceutical plant. Improvement of this genus through genetic transformation requires an efficient regeneration system, which is yet to be standardized. Taxonomic status of the genus is controversial and requires more inputs. Taxonomic delineation of species and subspecies and also the breeding programs can be more robustly addressed using molecular markers. This review summarizes the progress made and suggests some future directions of research for this important fruit species.

In vitro Comparative Antioxidant Activity of Ethanolic Extracts of Glycosmis pentaphylla , Bauhinia variegata and Leucas aspera

This study was conducted to investigate the antioxidant effect of the ethanolic extracts of the leaves of Glycosmis pentaphylla, Bauhinia variegata and the whole plant of Leucas aspera . The antioxidant activity was evaluated by various antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,22 -azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), nitric oxide and hydrogen peroxide (H 2 O 2 ) scavenging method. The antioxidant activities were compared to standard antioxidant ascorbic acid. Ethanolic crude extract of the plant Leucas aspera showed maximum significant antioxidant activity in DPPH, ABTS, nitric oxide and H 2 O 2 scavenging methods. The findings of the present study suggested that all the three plants could be a potential natural source of antioxidants and could have greater importance as therapeutic agent in preventing or slowing oxidative stress related degenerative diseases.

Wound healing activity of Glycosmis arborea leaf extract in rats

Glycosmis arborea Roxb, syn. Glycosmis pentaphylla Retz, (Rutacea) is a shrub found all over India, have been used for the healing of wounds of livestock in Indian folk medicine. The present study provides a scientific evaluation for the wound healing potential of ethanolic extract of Glycosmis arborea leaves. Excision and incision wounds were inflicted upon four groups containing six rats each. Group-1 was assigned as control (ointment base), Group-2 and Group-3 were treated with 5 and 10% EtOH extract ointment. Group-4 was treated with standard Nitrofurazone (0.2%) ointment. The parameters observed were percentage of wound contraction, epithelization period, tensile strength, hydroxyproline content. Ethanolic extract of Glycosmis arborea leaves was given orally at a dose of 200, 400mg/kg/day in dead space wound model. Topical application of Glycosmis arborea ointment (10%) in excision wound model significantly (P<0.05) increased the percentage of wound contraction (96.91%) compared with control (60.27%) and decreased the epithelization time. The tensile strength significantly (P<0.05) increased in Group-3 at 560.33 ± 6.48g when compared to control at 319.17±6.16 g. Rats treated with Glycosmis arborea extract (400mg/kg) showed significant (P<0.05) increase in hydroxyproline content at 54.94 ± 0.96 mg/g when compared with control at 30.77±1.13 mg/g. The ethanolic extract of Glycosmis arborea facilitated wound healing significantly, corroborating the folk medicinal use of this plant.

Hepatoprotective activity of Glycosmis pentaphylla against paracetamol-induced hepatotoxicity in Swiss albino mice

Context: Glycosmis pentaphylla (Retz.) DC (Rutaceae) is a shrub, traditionally used to treat anemia, rheumatism, as an anthelmintic, febrifuge and vermifuge, for jaundice, and liver complaints.Objective: This study evaluated the hepatoprotective activity of G. pentaphylla against paracetamol-induced hepatotoxicity in Swiss albino mice.Materials and methods: Effect of methanol extract (200 and 400 mg/kg) and petroleum ether extract (200 and 400 mg/kg) were studied on paracetamol-induced (250 mg/kg intraperitoneally) hepatic damage in mice for estimating the serum marker enzymes as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin, and total protein. Then the sections of liver were observed for histopathological changes in liver architecture including necrosis, steatosis, and fatty change of hepatic cells.Results: Mice were protected from the hepatotoxic action of paracetamol as evidenced by significant reduction in the elevated serum level of ALT (P < 0.001), AST (P < 0.001), ALP (P < 0.001), total bilirubin (P < 0.01) and an increased level of total protein (P < 0.01) with a significant reduction in liver weight (P < 0.01) when compared with paracetamol treated group and silymarin (50 mg/kg) was used as a positive control.Discussion and conclusion: These biochemical observations were supplemented by histopathological examination of liver sections from different experimental groups and corroborated the hepatoprotective efficacy of methanol and petroleum ether plant extract. The methanol extract (400 mg/kg) of G. pentaphylla is able to alter the toxic condition of the hepatocytes so as to protect the membrane integrity against paracetamol-induced leakage of marker enzymes.