Glycin Research Articles

Isolation of the drug-resistant Staphylococcus aureus strains from burn wound flora and analysis of norA genetic mutation

：Objective To investigatethe genetic mutation of the norA gene and its promotor from the wild-type drug-resistanceStaphylococeus aureus(S.aureus)strains. Methods A total of 10 antibiotic-resistantS.aureus strains were isolated and screened from the burn wound for the sequencing andanalysis of the nora gene and its promoter. Results There isolated 87 S.aureus strainsfrom the burn wound flora,which were completely sensitive to vacomycin,highly sensitive toQuinupristin and Nitrofurantoin,but highly resistant to the other antibiotics,even upto91.7% of MRSA.There found the same point mutation(G→A) located at 1 349 sites of the norA gene coding regionin all the S.aureus strains,saying that the amino acid was changed from Gly(glycin)toAsp(agpartic acid) in 291 sites.The resetpine reverse test showed that the MICs value ofthree antibiotics was lowered at various degrees in all 10strains.Conclusion NorA genemutation is one of the mechanisms for antibiotic-resistance of S.aureus. Key words: Burns; Staphylococcus aureus; Drug resistance; norAgene; Point mutation

SEQUENCIAMENTO E ANÁLISE DOS GENES DAS SUBUNIDADES ALFA E BETA DO HORMÔNIO FOLÍCULO ESTIMULANTE DE BOVINO (Bos taurus indicus)

The aim this work was to promove the cloning and analysis sequencing of the subunits alpha and beta of the Bos taurus indicus follicle stimulated hormone (FSH). It to compare the results of sequencing these subunits between subunits aplha and beta from swine and Bos taurus taurus previouly published in GenBank. There was a high similarity between nucleotides and predicted amino acids in the ?FSH chain of Bos taurus indicus and those of swine and buffalo. In the compare the sequence of the subunit of ?FSH of Bos taurus indicus with swine showed differences in three aminoacid residues with sFSH there was a modification in the first base of the codon, which had to an alteration in the 83 amino acid residue which in Bos taurus indicus and a glycin, this was serine in Bos taurus taurus. This modification, as well as those indentyfied in cDNA of the ?FSH and sFSH chains were confirmed by cloning. The modification of serine for glycine in position 83 was the only substitute that altered the residue in the comparson between sFSH subunit of Bos taurus indicus and Bos taurus taurus . Nevertheles this modification showld not significantly alter the physiological properties of FSH as the glycine residue was also found in the swine sFSH, it is therefore a specific modification which distinguishes between the sFSH of Bos taurus taurus and Bos taurus indicus. KEY WORDS: Bovine, cloning, FSH, hormone.

Simultaneous determination of paraquat and diquat in urine by capillary electrophoresis.

Qualitative and quantitative detection of paraquat and diquat in urine was performed by capillary zone electrophoresis (CZE). In non-extracted urine, these two closely related herbicides were hydrodynamically or electrokinetically injected into the capillary and well separated in a acidic (pH 3.0) buffer (10 mM glycin, 50 mM NaCl and 20% methanol) within 8 min. The analytes can be identified by their respective migration time with no interfering endogeous compounds around. The peaks were further confirmed by their respective multi-wavelength scanning spectra. Limits of detection (LOD) were 0.2 and 0.1 mg/ml for PQ and DQ, respectively, in nonextracted urine using hydrodynamic injection. Detection sensitivity was greatly improved by solid phase extraction of the compounds followed by CZE using electro-injection under which LODs were 15 and 8 ng/ml for paraquat and diquat respectively. We conclude CZE is a useful method for laboratory diagnosis of herbicide intoxication.

Amino Acids and Peptides; XLIII1. Dehydroamino Acids; XVIII2. Synthesis of Dehydroamino Acids and Amino Acids fromN-Acyl-2-(dialkyloxyphosphinyl)-glycin Esters; II

Synthese de deshydroaminoacides (I) et deshydropeptides (II) par condensation d'aldehydes avec des N-acyl- et N-alcoxycarbonyl-dialcoxyphosphoryl glycinates de methyle. Hydrogenation enantioselective de (I) et (II)