Abstract
：Objective To investigatethe genetic mutation of the norA gene and its promotor from the wild-type drug-resistanceStaphylococeus aureus(S.aureus)strains. Methods A total of 10 antibiotic-resistantS.aureus strains were isolated and screened from the burn wound for the sequencing andanalysis of the nora gene and its promoter. Results There isolated 87 S.aureus strainsfrom the burn wound flora,which were completely sensitive to vacomycin,highly sensitive toQuinupristin and Nitrofurantoin,but highly resistant to the other antibiotics,even upto91.7% of MRSA.There found the same point mutation(G→A) located at 1 349 sites of the norA gene coding regionin all the S.aureus strains,saying that the amino acid was changed from Gly(glycin)toAsp(agpartic acid) in 291 sites.The resetpine reverse test showed that the MICs value ofthree antibiotics was lowered at various degrees in all 10strains.Conclusion NorA genemutation is one of the mechanisms for antibiotic-resistance of S.aureus. Key words: Burns; Staphylococcus aureus; Drug resistance; norAgene; Point mutation
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