SEQUENCIAMENTO E ANÁLISE DOS GENES DAS SUBUNIDADES ALFA E BETA DO HORMÔNIO FOLÍCULO ESTIMULANTE DE BOVINO (Bos taurus indicus)

Abstract

The aim this work was to promove the cloning and analysis sequencing of the subunits alpha and beta of the Bos taurus indicus follicle stimulated hormone (FSH). It to compare the results of sequencing these subunits between subunits aplha and beta from swine and Bos taurus taurus previouly published in GenBank. There was a high similarity between nucleotides and predicted amino acids in the ?FSH chain of Bos taurus indicus and those of swine and buffalo. In the compare the sequence of the subunit of ?FSH of Bos taurus indicus with swine showed differences in three aminoacid residues with sFSH there was a modification in the first base of the codon, which had to an alteration in the 83 amino acid residue which in Bos taurus indicus and a glycin, this was serine in Bos taurus taurus. This modification, as well as those indentyfied in cDNA of the ?FSH and sFSH chains were confirmed by cloning. The modification of serine for glycine in position 83 was the only substitute that altered the residue in the comparson between sFSH subunit of Bos taurus indicus and Bos taurus taurus . Nevertheles this modification showld not significantly alter the physiological properties of FSH as the glycine residue was also found in the swine sFSH, it is therefore a specific modification which distinguishes between the sFSH of Bos taurus taurus and Bos taurus indicus. KEY WORDS: Bovine, cloning, FSH, hormone.