In this communication, an amperometric glucose biosensor based on MnO 2/MWNTs electrode was reported. MnO 2 was homogeneously coated on vertically aligned MWNTs by electrodeposition. The MnO 2/MWNTs electrode displayed high electrocatalytic activity towards the oxidation of glucose in alkaline solution, showing about 0.30 V negative shift in peak potential with oxidation starting at ca. −0.20 V (vs. 3 M KCl–Ag/AgCl) as compared with bare MWNTs electrode. At an applied potential of +0.30 V, the MnO 2/MWNTs electrode gives a linear dependence ( R = 0.995) in the glucose concentration up to 28 mM with a sensitivity of 33.19 μA mM −1. Meanwhile, the MnO 2/MWNTs electrode is also highly resistant toward poisoning by chloride ions. In addition, interference from the oxidation of common interfering species such as ascorbic acid, dopamine, and uric acid is effectively avoided. The MnO 2/MWNTs electrode allows highly sensitive, low-potential, stable, and fast amperometric sensing of glucose, which is promising for the development of nonenzymatic glucose sensor.