Abstract
In a series of studies on the physiology of corn, considerable attention has been given to the concentration of sugars in the different tissues of the plant, especially in relation to seasonal development. In these studies the total sugar content was differentiated into free reducing sugars and sucrose. It seemed desirable to carry the investigations a step further and determine the proportions of glucose and fructose which make up the free reducing sugars. Due to the similarity in chemical properties of these two sugars, however, procedures have not been standardized for the determination of either one of them in the presence of the other and sucrose in plant tissues. It consequently was necessary to make a preliminary survey of the available methods and to work out procedures which might be satisfactory for use with samples of corn tissues. Three of the general methods which have been used for determining glucose and fructose in mixtures of glucose, fructose, and sucrose are: (1) the oxidation of glucose in alkaline solution with iodine, (2) the oxidation of fructose with Nyns7 solution, a cupro-potassium carbonate solution, and (3) the method of analyzing a mixture of three sugars by determining the combined reducing power, polarization, and the direct determination of sucrose.
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