Global Response Research Articles

Book review: A. S. Bhalla, National and Global Responses to the COVID-19 Pandemic: Do Leaders Matter?

Book review: A. S. Bhalla, <i>National and Global Responses to the COVID-19 Pandemic: Do Leaders Matter?</i>

Climate change impacts on worldwide ecological niche and invasive potential of Sternochetus mangiferae.

Present climate studies on invasive species imply that climate change will alter the habitat suitability of invasive pests, especially given the projected rise in average global temperatures by the end of 2100. However, globally, limited information exists on the habitat suitability of the mango stone weevil, Sternochetus mangiferae Fabricius, which impedes the development of early detection and preventive measures. Herein, we used the MaxEnt model to estimate the potential global geographical distribution of S. mangiferae. Our results revealed that thermal conditions played a significant role in explaining the invasion risk of S. mangiferae. Habitat suitability was found in all continents, except Antarctica. Under the present condition, habitat suitability covered 5.67 × 107 km2. For ssp126, habitat suitability will decrease from the 2060s (5.58 × 107 km2) and 2080s (5.57 × 107 km2). Similarly, under ssp585, suitable areas will decrease from 5.62 × 107 to 5.51 × 107 km2 for the 2060s and 2080s, respectively. Our study has estimatedvariability in the habitat suitability of S. mangiferae which establishes a foundation for determining global riskassessment and response plans for the pest. This study also identifies areas where the pest is inherently more vulnerable to the impacts of changing climates and enables forecasting of its potential distribution in a dynamic world. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Brain Abnormalities in Young Single- and Double-Heterozygote Mice for Both Nkx2-1- and Pax8-Null Mutations.

In humans and mice, Nkx2-1 and Pax8 are crucial morphogenic transcription factors defining the early development of the thyroid and specific extrathyroidal tissues. By using 3-month-old single or double heterozygotes for Nkx2-1- and Pax8-null mutations (DHTP) mice, we studied brain abnormalities under different human-like dysthyroidisms, focusing on putative alterations of specific neurotransmitter systems, expression of markers of pre- and post-synaptic function and, given the physio-pathological role mitochondria have in controlling the bioenergetic status of neurons, of mitochondrial dynamics and oxidative balance. Compared to Wt controls, DHTP mice, bearing both systemic and brain hypothyroidism, showed altered expression of synaptic markers, generic and cholinergic (corroborated by immunohistochemistry in caudate, putamen, hippocampus, and basal forebrain) and glutamatergic ones, and reduced expression of key proteins of synaptic plasticity potency and several isoforms of glutamate receptors. The brain of DHTP mice was characterized by lower levels of H2O2 and imbalanced mitochondrial dynamics. Nkx2-1 + / - mice showed dopaminergic neuron-specific alterations, morphologically, more evident in the substantia nigra of DHTP mice. Nkx2-1 + / - mice also showed enhanced mitochondrial biogenesis and oxidative capacity likely as a global response of the brain to Nkx2-1 haploinsufficiency and/or to their elevated T3 circulating levels. Reduced transcription of both tyrosine hydroxylase and dopamine transporter was observed in Pax8 + / - euthyroid mice, suggesting a dopaminergic dysfunction, albeit likely at an early stage, but consistent with the deregulated glucose homeostasis observed in such animals. Overall, new information was obtained on the impact of haploinsufficiency of Pax8 and NKx2-1 on several brain neuroanatomical, molecular, and neurochemical aspects, thus opening the way for future targeting brain dysfunctions in the management of both overt and subclinical thyroid dysfunctions.

Impact of Surface Finishing on Ti6Al4V Voronoi Additively Manufactured Structures: Morphology, Dimensional Deviation, and Mechanical Behavior

Additively manufactured medical devices require proper surface finishing before their use to remove partially adhered particles and provide adequate surface roughness. The literature widely investigates regular lattice structures—mainly scaffolds with small pores to enhance osseointegration; however, only a few studies have addressed the impact of surface finishing on the dimensional deviation and the global and local mechanical responses of lattice samples. Therefore, the current research investigates the impact of biomedical surface finishing (i.e., corundum sandblasting and zirconia sandblasting) on Voronoi lattice structures produced by laser powder bed fusion (LPBF) with large pores and different thicknesses on the surface morphology and global and local mechanical behaviors. MicroCT and SEM are performed for the assessment of dimensional mismatch and surface evaluation. The mechanical properties are investigated with 2D digital image correlation (DIC) in quasi-static compression tests to estimate the impact of surface finishes on local maps of strain. In the quasi-static tests, both the global mechanical performances, as expected, and local 2D DIC strain maps were mainly affected by the strut thickness, and the impact of different surface finishings was irrelevant; on the contrary, different surface finishing processes led to differences in the dimensional deviation depending on the strut thickness. These results are relevant for designing lattice structures with thin struts that are integrated into medical prostheses that undergo AM.

Solidarity for the mpox outbreak: A call for a unified global response

No abstract available.

Evaluation of an Affinity-Enhanced Anti-SARS-CoV2 Nanobody Design Workflow Using Machine Learning and Molecular Dynamics.

In silico optimization of protein binding has received a great deal of attention in the recent years. Since in silico prefiltering of strong binders is fast and cheap compared to in vitro library screening methods, the advent of powerful hardware and advanced machine learning algorithms has made this strategy more accessible and preferred. These advances have already impacted the global response to pandemic threats. In this study, we proposed and tested a workflow for designing nanobodies targeting the SARS-CoV-2 spike protein receptor binding domain (S-RBD) using machine learning techniques complemented by molecular dynamics simulations. We evaluated the feasibility of this workflow using a test set of 3 different nanobodies and 2 different S-RBD variants, from in silico design and bacterial expression to binding assays of the designed nanobody mutants. We successfully designed nanobodies that were subsequently tested against both the wild-type (Wuhan type) and the delta variant S-RBD and found 2 of them to be stronger binders compared to the wild-type nanobody. We use this case study to describe both the strengths and weaknesses of this in silico assisted nanobody design strategy.

Engineering Escherichia coli for l-Threonine Hyperproduction Based on Multidimensional Optimization Strategies.

Exploring effective remodeling strategies to further improve the productivity of high-yield strains is the goal of biomanufacturing. However, the lack of insight into host-specific metabolic networks prevents timely identification of useful engineering targets. Here, multidimensional engineering strategies were implemented to optimize the global metabolic network for improving l-threonine production. First, the metabolic bottleneck for l-threonine synthesis was eliminated by synergistic utilization of NADH and an enhanced ATP supply. Carbon fluxes were redistributed into the TCA cycle by rationally regulating the GltA activity. Subsequently, the stress global response regulator UspA was identified to enhance l-threonine production by a transcriptomic analysis. Then, l-threonine productivity was improved by enhancing the host's stress resistance and releasing the inhibitory reaction of glucose utilization. Eventually, the l-threonine yield of THRH16 reached 170.3 g/L and 3.78 g/L/h in a 5 L bioreactor, which is the highest production index reported. This study provides rational guidance for increasing the productivity of other chemicals.

Developing Training in Response to High-Consequence Infectious Diseases and Preparedness Measures for the Future.

In recent history, outbreaks of high-consequence infectious diseases (HCIDs) have raised health security concerns among the global community. As HCIDs continue to emerge, public health systems around the world experience the burden of implementing adequate preparedness and response measures to ensure the safety and security of their populations. HCID outbreak response efforts have highlighted the need for specialized training in safety and infection prevention and control for frontline workers who may encounter ill patients. The COVID-19 Mission Prep program for National Disaster Medical System personnel and the Deployment Safety Academy for Field Experiences (D-SAFE) program for US Public Health Service officers are 2 examples of virtual training programs that successfully provided foundational education on infection prevention and control and safety as well as deployable just-in-time training during HCID outbreak response efforts. The methods used to develop these training programs can be adopted by other countries to enhance the global outbreak response infrastructure for the next HCID event. The global outbreak response infrastructure demands investments in training as a preparedness measure, which will ultimately lead to safer, more coordinated outbreak response efforts with competent responders.

Method of generating potential evapotranspiration with high precision and resolution

Potential evapotranspiration (PET) is a key factor in hydrological cycle and energy balance and plays an important role in drought and global climate change response. Existing observational and modeling methods for PET retrieval have their limitations, such as low precision and poor spatial resolution, which becomes the focus of this study. A hybrid PET fusion (HPF) method is proposed by fusing station- and grid-based PET, in which the PET expression is determined by considering the factors of location, temperature, and zenith total delay (ZTD). In addition, an improved Helmert variance component estimation method is introduced to determine the optimal weights of the HPF model. Corresponding data, which include monthly Thornthwaite (TH)-derived PET data with a spatial resolution of 0.25° × 0.25° and Penman–Monteith (PM)-derived PET data at 704 meteorological stations, over the past 60 years from 1959 to 2018 in China are selected. The 10-fold cross-validation method is introduced to evaluate the internal and external accuracies of the proposed HPF method. Statistical result shows that the average root mean square (RMS) of the proposed HPF method is 13.98 mm, with an average RMS improvement rate (IR) of 46.71 % compared with TH-derived PET, when PM-derived PET is regarded as a reference. Moreover, the performance of the HPF-derived standardized precipitation evapotranspiration index (SPEI) is evaluated at different time scales, and the average RMS is 0.3, with an average RMS IR of 26.33 % compared with TH-derived SPEI. Such results verify the good performance of the proposed HPF model and enrich the methods for obtaining PET with high precision and resolution.

Refugees’ employment: Adapting a structural inequality framework for multinational corporations

Multinational corporations (MNCs), through action or inaction, directly and indirectly shape vertical, horizontal, or internal structural inequalities in labour markets. The refugee employment literature has given scant attention to the role of MNCs, and how these corporations can or do contribute to achieving equality in employment outcomes for diverse social groups such as refugees. In this paper, we explore how MNCs seek to address challenges associated with structural inequalities to achieve greater equality in employment outcomes for refugees. To do so, we draw on the refugee employment literature, industry evidence, and business reports. Our findings reveal that MNCs require global and national-level responses to address structural inequalities associated with vertical, horizontal, or internal inequalities. We synthesise these inequalities into a conceptual framework and illustrate the need for MNCs to balance standardisation vs localisation strategies in human resource and diversity management, sourcing and supply chain management, corporate social responsibility, marketing and branding, and external engagement. Such MNC strategies contribute to refugees’ ability to navigate structural factors that create employment inequalities.

Comparative Transcriptomic Analyses Reveal Differences in the Responses of Diploid and Triploid Eastern Oysters to Environmental Stress.

Triploid oysters are commonly used as the basis for production in the aquaculture of eastern oysters along the USA East and Gulf of Mexico coasts. While they are valued for their rapid growth, incidents of triploid mortality during summer months have been well documented in eastern oysters, especially at low salinity sites. We compared global transcriptomic responses of diploid and triploid oysters bred from the same three maternal source populations at two different hatcheries and outplanted to a high (annual mean salinity = 19.4 ± 6.7) and low (annual mean salinity = 9.3 ± 5.0) salinity site. Oysters were sampled for gene expression at the onset of a mortality event in the summer of 2021 to identify triploid-specific gene expression patterns associated with low salinity sites, which ultimately experienced greater triploid mortality. We also examined chromosome-specific gene expression to test for instances of aneuploidy in experimental triploid oyster lines, another possible contributor to elevated mortality in triploids. We observed a strong effect of hatchery conditions (cohort) on triploid-specific mortality (field data) and a strong interactive effect of hatchery, ploidy, and outplant site on gene expression. At the low salinity site where triploid oysters experienced high mortality, we observed downregulation of transcripts related to calcium signaling, ciliary activity, and cell cycle checkpoints in triploids relative to diploids. These transcripts suggest dampening of the salinity stress response and problems during cell division as key cellular processes associated with elevated mortality risk in triploid oysters. No instances of aneuploidy were detected in our triploid oyster lines. Our results suggest that triploid oysters may be fundamentally less tolerant of rapid decreases in salinity, indicating that oyster farmers may need to limit the use of triploid oysters to sites with more stable salinity conditions.

STOP1 regulates CCX1-mediated Ca2+ homeostasis for plant adaptation to Ca2+ deprivation.

Calcium (Ca) is essential for plant growth and stress adaptation, yet its availability is often limited in acidic soils, posing a major threat to crop production. Understanding the intricate mechanisms orchestrating plant adaptation to Ca deficiency remains elusive. Here, we show that the Ca deficiency-enhanced nuclear accumulation of the transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) in Arabidopsis thaliana confers tolerance to Ca deprivation, with the global transcriptional responses triggered by Ca deprivation largely impaired in the stop1 mutant. Notably, STOP1 activates the Ca deprivation-induced expression of CATION/Ca2+ EXCHANGER 1 (CCX1) by directly binding to its promoter region, which facilitates Ca2+ efflux from endoplasmic reticulum to cytosol to maintain Ca homeostasis. Consequently, the constitutive expression of CCX1 in the stop1 mutant partially rescues the Ca deficiency phenotype by increasing Ca content in the shoots. These findings uncover the pivotal role of the STOP1-CCX1 axis in plant adaptation to low Ca, offering alternative manipulating strategies to improve plant Ca nutrition in acidic soils and extending our understanding of the multifaceted role of STOP1.

Global Governance under Populism: The Challenge of Information Suppression

abstract: Populists’ ideological opposition to global governance is well recognized, yet whether and how these actors systematically undermine international organizations remain unclear. The authors argue that a key means by which populists warp global governance is by distorting scientific information, which is necessary for global responses to many public health and environmental issues. Populists are motivated to withhold or misreport scientific information due to their anti-elite, prostate sovereignty views. Using new data on the source and quality of information provided to international organizations (ios), the authors find that populist leaders are significantly less likely to provide scientific information to ios than are other types of leaders. When they do offer such data, they are less accurate than the information that other sources supply. The authors’ findings suggest that populism may stymie international institutions’ ability to govern in areas of pressing international concern.

Policy and practice review consensus statements and clinical guidelines on managing pediatric trauma and orthopedics during the COVID-19 pandemic: a systematic review on the global response for future pandemics and public health crises.

The COVID-19 pandemic has been recognized as an unprecedented global health crisis. Over 7 million mortalities have been documented with many paediatric fatalities. Trauma and orthopaedic care, much like other specialities, were marginalized due to resource allocation during the pandemic which affected paediatric care. This is the first systematic review to centralise and compile the recommended published guidelines from professional bodies in principally English speaking countries on managing paediatric trauma and orthopaedic care. These guidelines will be required to be implemented sooner and more effectively in case of future pandemics with similar impact. A search was conducted on PubMed/MedLine, Cochrane Library and Embase using terms including p(a)ediatric or child* and/or COVID* or coronavirus or SARS-CoV-2 and/or trauma and/or orthop(a)edic* with a simplified MeSH heading [mh] in order to make the search as comprehensive as possible. General terminology was utilized to make the search as exhaustive as possible for this systematic review. Another search was conducted on resources available in the public domain from professional bodies publishing on consensus statements and clinical practice guidelines in countries where English is the principal language managing pediatric trauma and orthopedics. The review adhered to PRISMA guidance. The search revealed a total of 62 results from both databases and professional bodies. Duplicates were removed. This was then reviewed to identify a total of 21 results which fit the inclusion criteria and included within the main analysis. The guidelines from professional bodies were outlined and categorized into aspects of clinical care. The impact of COVID-19 pandemic has compelled for changes in clinical practice and pediatric management. The systematic review highlights the relevant guidelines on service provision for pediatric patients including indications for urgent referrals, surgical prioritization, reasons for follow-up and trauma guidelines. The rationale for care during the unpredictable evolution of the COVID-19 pandemic may have the potential to be translated and replicated in future pandemics of similar significance.

Seizing the moments and lessons learned from the global response to COVID-19 pandemic: Creating a platform to shape the scientific and public discourse of research on adolescence.

COVID-19 response offers a model to guide research and preventive interventions targeting adolescents, their families, and communities. My 2022 SRA Presidential Address posed: What if the COVID-19 Response Served as a Guidepost for Future Research on Adolescence? Solution versus Problem-Focused Agenda. Several "pandemics" were already underway, emerging from historic and contemporary events that threaten the safety and survival of human lives. The Multi-Transgenerational Life Course Theoretical model was selected to demonstrate pathways through which the transmission of generational exposure to crisis and trauma impact adolescents' developmental trajectories. Recommendations to inform and guide an adolescent research rapid response agenda are proposed minds to advance equity and social justice can become realities.

Influence of budesonide and fluticasone propionate in the anti-osteoporotic potential in human bone marrow-derived mesenchymal stem cells via stimulation of osteogenic differentiation

Osteoporosis is a prevalent bone condition with adverse effects observed in patients undergoing long-term glucocorticoid therapy, resulting in bone demineralization and tissue loss. There has been limited studies on the global response to dexamethasone in terms of comparing its expression profile to other common glucocorticoids during osteogenic differentiation. This study focused on the downregulated gene expression profile of glucocorticoid compounds; dexamethasone, budesonide, and fluticasone propionate, during osteogenic differentiation to elucidate the related target genes and pathways associated with the anti-osteoporotic potential of telomerase-immortalized human bone marrow-derived mesenchymal stem cells using a bioinformatics approach. Based on gene expression microarrays experiments and bioinformatics analysis, several key genes involved in the regulation of osteogenic differentiation and osteoporosis development in mesenchymal stem cells that were targeted by these specific glucocorticoids were determined. Network analysis using GeneCards, OMIM, and CTD databases were performed and osteoporosis-related genes were identified. LIMMA and moderated Welch test R packages were performed to determine significant downregulated differentially expressed genes for each glucocorticoid treatment. A total of 479 (dexamethasone), 84 (budesonide), and 889 (fluticasone propionate) differentially expressed genes were identified for each glucocorticoid, of which 35 common genes overlapped. Enrichment pathway analysis was conducted using Metascape, and protein-protein interaction networks were constructed using the STRING database and Cytoscape software to determine potential target genes involved with osteoporosis. Enrichment pathway analysis revealed genes involved in 3 Reactome pathways namely cytokine signaling in immune system, immune system and the interferon alpha/beta signaling pathways and identified 10 hub genes based on the PPI network to determine potential target pathways associated with osteoporosis. These findings provide preliminary insights into the relationship between the key target genes of dexamethasone, budesonide, and fluticasone propionate, and the pathways associated with regulated osteoporosis metabolism during osteogenic differentiation.

Reorganizing public value for city life in the Anthropocene

Public value and city governance are fundamental notions in contemporary settings, but, currently conceived, they are not fit for the challenges presented by the proposed new epoch of geological time—the Anthropocene. Walking through the locked-down streets or calle of Venice, we face the sudden emptiness that starkly reveals the impact of human activity on the city and its waterways. Reflecting on the walk, our starting point is to problematize how a city organizes and manages public value and what actually constitutes public value. In this, we develop a new definition, “New Public Value for the Anthropocene Epoch” (NPVA), which expands the notion of public value through the questions: “who” is it valuable to do things for, beyond humans and economic actors, building on a relational epistemology to incorporate the planet and its biosphere; and “what” is valuable to do, in order to ensure the inclusion of social, environmental, and cultural values alongside economic values. We conclude by arguing that NPVA is organized across scales in a manner that embeds global attentiveness toward local ecosystems solutions to drive the global response to the environmental crisis we all face.

Comparative Analysis for Bioremediation of Plastic and Dye Degradation

The leading environmental risk factor for disease and premature death is pollution, with plastic and dye pollutants being the most common in developing countries. The global textile industry contributes to pollution by releasing contaminated wastewater into water bodies, leading to a decline in water quality. Plastic pollution is a widespread issue affecting various environments, emphasizing the urgent need for a global response to combat the adverse effects of pollution on human health and the environment. Anoxybacillus sp. PDR2 is a soil bacterium possessing natural competence. By nature, it is a thermophile, capable of biodegrading industrial dyes. Pseudomonas sp. B10, Gram-negative bacteria, is a strain capable of degrading Polyethylene Terephthalate (PET) plastic. To detect the genome-level mutations, comparative genomic analysis was performed using a free and open source software, Galaxy. Using five different variant callers (Samtools, Varscan, Freebayes, Sniffles, Ivar), mutations were detected at various loci resulting in the modifications of the genes. The primary goal of this investigation was to perform a comparative analysis of the whole genome sequencing of two bacterial species, along with their reference strains. The purpose was to identify potential solutions for the degradation of plastics and industrial dyes. By examining the genetic composition of these bacteria, this analysis had provided valuable insights into the genetic makeup of these bacteria and their ability to break down PETs and dyes.

It Takes Two to Make a Thing Go Right: Epistasis, Two-Component Response Systems, and Bacterial Adaptation

Understanding the interplay between genotype and fitness is a core question in evolutionary biology. Here, we address this challenge in the context of microbial adaptation to environmental stressors. This study explores the role of epistasis in bacterial adaptation by examining genetic and phenotypic changes in silver-adapted Escherichia coli populations, focusing on the role of beneficial mutations in two-component response systems (TCRS). To do this, we measured 24-hour growth assays and conducted whole-genome DNA and RNA sequencing on E. coli mutants that confer resistance to ionic silver. We showed recently that the R15L cusS mutation is central to silver resistance, primarily through upregulation of the cus efflux system. However, here we show that this mutation’s effectiveness is significantly enhanced by epistatic interactions with additional mutations in regulatory genes such as ompR, rho, and fur. These interactions reconfigure global stress response networks, resulting in robust and varied resistance strategies across different populations. This study underscores the critical role of epistasis in bacterial adaptation, illustrating how interactions between multiple mutations and how genetic backgrounds shape the resistance phenotypes of E. coli populations. This work also allowed for refinement of our model describing the role TCRS genes play in bacterial adaptation by now emphasizing that adaptation to environmental stressors is a complex, context-dependent process, driven by the dynamic interplay between genetic and environmental factors. These findings have broader implications for understanding microbial evolution and developing strategies to combat antimicrobial resistance.

Money Laundering: Impact on Financial Institutions and International Law-A Systematic Literature Review

This study investigates the impact of money laundering on financial institutions and the effectiveness of international legal frameworks in combating this pervasive issue. Utilizing a systematic literature review methodology, the research analyzes key themes, including vulnerabilities of financial institutions, the efficacy of regulatory frameworks, the consequences of money laundering, and the evolving techniques employed by criminals. The findings reveal significant weaknesses in compliance measures within financial institutions, leading to reputational damage and substantial financial penalties. Additionally, while international frameworks like the FATF’s recommendations provide essential guidelines, inconsistent compliance and outdated regulations hinder their effectiveness. The consequences of money laundering extend beyond individual institutions, distorting market dynamics and exacerbating inequality, particularly in jurisdictions with weak regulatory systems. The study highlights the urgent need for enhanced collaboration among stakeholders, proactive regulatory adaptations, and a comprehensive approach that integrates technology and intelligence sharing. Ultimately, the research underscores the importance of a coordinated global response to effectively address the challenges posed by money laundering, contributing to the integrity and stability of the financial system.