A survey was conducted to assess the quality of groundnut seed produced and stored under ambient environment across various agro-ecologies of The Gambia, with a view to understanding regions with comparative advantage for its production and storage. Seed production activities of 60 seed growers in 26 communities were collected using structured questionnaires, interviews and observations. The Global Positioning System (GPS) coordinates of the communities were geo-referenced to identify the geographical positioning of the production sites. Samples of the groundnut pods were taken from the seed stores, threshed and subjected to seed quality analysis. Descriptive analysis was used to categorize the seed sources, hectarage cultivated, while seed quality data were subjected to analysis of variance of Nested Design. Means of significant factors were separated using Duncan Multiple Range Test (DMRT) at 5% significant level. The study showed that groundnut seed production is better concentrated in regions with optimum and sustainable rainfall that will reduce abiotic stress during seed development like the Lower River Region, West Coast Region and Central River Region. Fleur-11 variety was identified as one of the most promising varieties for groundnut cultivation in The Gambia and Tropical Africa.