Spilanthol presents anesthetic, antiseptic and anti-inflammatory capacities, as well as anti-microbial and insecticidal activities. In this work, the supercritical carbon dioxide (scCO2) extraction kinetics of spilanthol from jambu flowers was studied by changing the solvent density (700–900kgm−3) at different temperatures (323–343K). Spilanthol was isolated from the obtained extracts by TLC and identified/quantified by GC-FID/MS. The highest global extraction yield (∼9%) was obtained at 900kgm−3 and 343K, while the highest spilanthol yield and selectivity (2.6% and 34.6%, respectively) were obtained at 800kgm−3 and 343K. Indirect values for the solubility of spilanthol in scCO2 were estimated from the spilanthol extraction kinetic curves and ranged between (0.7–2.6)×10−3gspilanthol/LCO2(PTN). The high selectivity of the extraction process for spilanthol achieved in the present work indicates potential advantages of using scCO2 to obtain spilanthol from S. acmella.