Introduction : 3D anthropomorphic models of human tissues have become a requirement for conducting realistic virtual studies. One of the current directions in the research of X-ray imaging is the development of physical models with 3D printing techniques using specific materials aiming to obtain replica of the human body tissues with similar radiological characteristics. Aim : The aim of this study is to create a calibration phantom for establishing the X-ray properties of different cartridge infills and their suitability to represent the X-ray properties of different breast types. Materials and Methods : A physical calibration model consisting of 22 objects was designed and printed by using an inkjet printer. A mixture was obtained from 5 mL printer ink and 3 g of potassium iodide (KI), which was used to fill the printer’s cartridge and to print the model on a set of plain office paper. Experimental X-ray images of the physical model were acquired on radiographic system SEDECAL X PLUS LP+. The obtained attenuation coefficient of the printing mixture was evaluated and compared to the breast tissue coefficients corresponding to the used X-ray energy. Results and Discussion : The physical model was printed on ten office sheets and stacked above one another. The obtained attenuation coefficient of the printing mixture was found very similar to that of the glandular tissue of the breast for the used X-ray energy. Conclusion: The obtained printer ink-KI mixture is suitable for representing the glandular part of breast tissue. The method has the potential to be used for creation of a realistic physical breast model.